scholarly journals First Record of Trichodorus primitivus and Morphological and Molecular Identification of Longidorus elongatus from Canada

Plant Disease ◽  
2010 ◽  
Vol 94 (6) ◽  
pp. 782-782 ◽  
Author(s):  
M. Pedram ◽  
G. Niknam ◽  
R. T. Robbins ◽  
W. Decraemer ◽  
W. Ye ◽  
...  

During July 2008, sandy loam soil samples were collected near the rhizosphere of Kentucky bluegrass (Poa pratensis L.) and tall fescue (Festuca arundinacea Schreb.) at a depth of 15 to 20 cm from Caledonia Park, Toronto, Canada. Samples were suspended in water and nematodes were collected on sieves with 250- and 74-μm openings. Among the nematodes recovered were one species of Longidorus Micoletzky, 1922 (4) and one species of Trichodorus Cobb, 1913. The Trichodorus species (2), was identified as T. primitivus (de Man, 1880) Micoletzky, 1922, in which females have rod-like sclerotized pieces parallel to the vagina lumen, each with dimensions of 3.1 × 1.2 μm and at a distance of 2 to 3 μm from each other; and males have spicules with a wide nonoffset capitulum and very narrow blade, three ventromedian cervical papillae (CP), and three precloacal supplements with the anterior one at the level of the capitulum of retracted spicules. Males showed unusual variation in the position of CP2 (i.e., posterior to the onchiostyle region, except for one specimen with CP2 located within the posterior onchiostyle region, which is typical for the species). The morphometric data for nine males are: L, 665 to 805 μm; a, 23.0 to 27.5; b, 3.7 to 5.2; c, 46 to 56; onchiostyle, 47 to 52 μm; and spicules, 32.5 to 40.5 μm. Measurements for 13 females are: L, 630 to 775 μm; a, 20.5 to 24.8; b, 3.5 to 5.5; c, 75.5 to 158.5; and V, 54 to 61%. To our knowledge, this is the first report of T. primitivus in Canada. According to Chen et al. (1), the Longidorus species was identified as L. elongatus (de Man, 1876) Micoletzky, 1922 (3). The morphometric data for females (n = 13) are: L, 5.1 to 6.0 mm; a, 78.5 to 106.5; b, 11.5 to 13.5; c, 86.0 to 120.5; V, 45.5 to 53.0%; odontostyle, 79 to 91 μm; odontophore, 57.5 to 66.5 μm; and tail length, 44.5 to 59.0 μm. Males were not found. Four juvenile stages were identified. J1 (n = 2) with a body length of 1.1, 1.2 mm and replacement/functional odontostyle 59, 59/53.5, 54.5 μm, J2 (n = 2) with L: 1.7, 2.0 mm and replacement/functional odontostyle 62.5, 68.0/58.7 μm, 59.0, J3 (n = 2): L: 2.8, 3.0 mm and replacement/functional odontostyle 76.5, 77/66.5, 67.0 μm and J4 (n = 2) with L: 3.6, 3.8 mm and replacement/functional odontostyle 87.0, 90.5/75.0, 77.5 μm. Due to large morphometric overlap in Longidorus species identification, 2,472 bp of the near full-length 18S and the internal transcribed spacer 1 region of rDNA (Accession No. GU199044) were sequenced. The Blastn search of the partial 18S revealed 100% identity with a population of L. elongatus from Scotland (GenBank No. AY687992, 1,707 bp compared), 99% identity (3 bp difference, 1,707 bp compared) with a population of L. elongatus from Iran (EU503141) and 99% identity (4 bp difference, 1,707 bp compared) with a population of L. elongatus (AF036594, sample location unknown). A Blastn search of the 18S and ITS region revealed only 1 to 3 bp differences with two populations of L. elongatus from Switzerland (AJ549986 and AJ549987) and a population of L. elongatus (AF511417) from Scotland. These molecular data further confirmed the identity of the population from Canada to be L. elongatus. References: (1) Q. Chen et al. Fundam. Appl. Nematol. 20:15, 1997. (2) W. Decraemer. Kluwer Academic Publishers, Dordrecht, the Netherlands, 1995. (3) J. G. de Man. Tijdschr. Ned. Dierk. Ver. 2:78, 1876. (4) H. Micoletzky. Archiv. Naturgesch. 87:1, 1922.

1990 ◽  
Vol 115 (4) ◽  
pp. 608-611 ◽  
Author(s):  
Jennifer M. Johnson-Cicalese ◽  
C.R. Funk

Studies were conducted on the host plants of four billbug species (Coleoptera:Curculionidae: Sphenophorus parvulus Gyllenhal, S. venatus Chitt., S. inaequalis Say, and S. minimus Hart) found on New Jersey turfgrasses. A collection of 4803 adults from pure stands of various turfgrasses revealed all four billbugs on Kentucky bluegrass (Poa pratensis L.), tall fescue (Festuca arundinacea Schreb.), and perennial ryegrass (Lolium perenne L.), and S. parvulus, S. venatus, and S. minimus on Chewings fescue (F. rubra L. ssp. commutata Gaud.). Since the presence of larvae, pupae, or teneral adults more accurately indicates the host status of a grass species, immature billbugs were collected from plugs of the various grass species and reared to adults for identification. All four species were reared from immature billbugs found in Kentucky bluegrass turf; immatures of S. venatus, S. inaequalis, and S. minimus were found in tall fescue; S. venatus and S. minimus in perennial ryegrass; and S. inaequalis in strong creeping red fescue (F. rubra L. ssp. rubra). A laboratory experiment was also conducted in which billbug adults were confined in petri dishes with either Kentucky bluegrass, perennial ryegrass, tall fescue, or bermudagrass (Cynodon dactylon Pers.). Only minor differences were found between the four grasses in billbug survival, number of eggs laid, and amount of feeding. In general, bermudagrass was the least favored host and the other grasses were equally adequate hosts. The results of this study indicate a need for updating host-plant lists of these four billbug species.


Plant Disease ◽  
2006 ◽  
Vol 90 (2) ◽  
pp. 246-246
Author(s):  
G. Polizzi ◽  
A. Vitale ◽  
I. Castello

Tall fescue (Festuca arundinacea Schreb.) and Kentucky bluegrass (Poa pratensis L.) are the main turfgrass species cultivated in Sicily (southern Italy) for ready lawn (sod) to ornamental purposes. In July 2004 and May 2005, a widespread disease was noticed in two turf nurseries on the eastern side of Sicily on a ready lawn mixture of F. arundinacea cv. Safari (94%) + P. pratensis cv. Cabaret (6%). Numerous yellow, circular- and crescent-shaped patches as much as 30 to 40 cm in diameter were observed. The turf usually died around the perimeter of the patch, but the grass remained green in the center of the ring with a tuft of green grass in the center (frog eye). Affected turf was initially reddish brown and turned brown as it died. Small, round and off-white or tan seed-like structures were dispersed on mycelial strands at the outer edge of the ring in the mat at the base of grasses. The pathogen was identified as Sclerotium rolfsii Sacc. The fungus was isolated directly as aerial mycelium or sclerotia or following surface disinfection (2 min in 0.5% NaOCl) and plating diseased tissues on potato dextrose agar (PDA). Sclerotia were observed in vitro in 7-day-old cultures. Pathogenicity was tested by inoculating two com-mercial ready lawn strips (80 × 100 cm) of two healthy turfgrass species each with three isolates of the fungus. Thirty sclerotia were placed at the base of stems. Noninoculated ready lawn strips served as control. All plants were covered with plastic bags, exposed to diffused daylight for 5 days, and then maintained in a growth chamber at 25 to 28°C under fluorescent light. Disease symptoms and southern blight signs like the ones observed in the field occurred 2 weeks after inoculation. S. rolfsii was reisolated from affected tissues. Symptoms were not detected on any of the non-inoculated ready lawn strips. The disease was serious enough that chemical treatments were required for its control. Southern blight was previously detected on bermudagrass and other cool-season turfgrass genera (1).To our knowledge, this is the first report of southern blight on tall fescue and bluegrass in Italy. Reference: (1) R. W. Smiley. Common Names of Plant Diseases. Diseases of Turfgrasses. Online publication. The American Phytopathological Society, St. Paul, MN.


2017 ◽  
Vol 2 (3) ◽  
pp. 162-170
Author(s):  
Kenneth Lynn Diesburg ◽  
Ronald F. Krausz

This research was conducted to determine the degree of success, by month, in seeding establishment of tall fescue (Festuca arundinacea Schreb., Kentucky bluegrass (Poa pratensis L.), Bermudagrass (Cynodon dactylon [L.] Pers. var. dactylon), and zoysiagrass (Zoysia japonica Steud.) at two locations in the moist, Midwest, continental transition zone on a prepared seed bed without irrigation or cover. The four species were planted every month of the year starting in September 2005. Starter fertilizer and siduron were applied the same day as seeding with no subsequent management except mowing. Percent cover of living turfgrass was recorded in each of 24 months after seeding. Tall fescue (80%) and Bermudagrass (73%) provided the best percent cover over all planting dates. Kentucky bluegrass provided 65% and zoysiagrass 24% cover. The cool-season grasses performed best in the July-to-March plantings; tall fescue 88% and Kentucky bluegrass 72%. Bermudagrass (94%) established best in the January-to-April plantings, while Zoysiagrass (32%) established best in the November-to-March plantings. Germination and seedling survival after germination of all species were inhibited by limited moisture during summer. The warm-season grasses were further limited by winter kill in the August, September, and October seedings. These results emphasize the risk in spring-seeding as well as the value in dormant-seeding of both warm- and cool-season turfgrasses for low-input, nonirrigated establishment.


2001 ◽  
Vol 11 (1) ◽  
pp. 152a
Author(s):  
Zachary J. Reicher ◽  
Clark S. Throssell ◽  
Daniel V. Weisenberger

Little documentation exists on the success of seeding cool-season turf-grasses in the late fall, winter and spring. The objectives of these two studies were to document the success of seeding Kentucky bluegrass (Poa pratensis L.), perennial ryegrass (Lolium perenne L.), and tall fescue (Festuca arundinacea Schreb.) at less-than-optimum times of the year, and to determine if N and P fertilizer requirements vary with seeding date of Kentucky bluegrass. `Ram I' Kentucky bluegrass, `Fiesta' perennial ryegrass, and `Mustang' tall fescue were seeded on 1 Sept., 1 Oct., 1 Nov., 1 Dec., 1 Mar., 1 Apr., and 1 May ± 2 days beginning in 1989 and 1990. As expected, the September seeding date produced the best establishment, regardless of species. Dormant-seeding Kentucky bluegrass and tall fescue in November, December, or March reduced the establishment time compared with seeding in April or May. Seeding perennial ryegrass in November, December, or March may not be justified because of winterkill potential. To determine the effect of starter fertilizer on seedings made at different times of the year, `Ram 1' Kentucky bluegrass was seeded 1 Sept., 1 Nov., 1 Mar., and 1 May ± 2 days in 1989 and 1990, and the seedbed was fertilized with all combinations of rates of N (0, 24, and 48 kg·ha-1) and P (0, 21, and 42 kg·ha-1). Fertilizer rate had no effect on establishment regardless of seeding date, possibly because of the fertile soil on the experimental site.


HortScience ◽  
1990 ◽  
Vol 25 (4) ◽  
pp. 449-451
Author(s):  
J.M. Goatley ◽  
A.J. Powell ◽  
W.W. Witt ◽  
M. Barrett

Chlorsulfuron, diclofop, and sulfometuron were evaluated for potential use in selective control of tall fescue (Festuca arundinacea Schreb.) in Kentucky bluegrass (Poa pratensis L.). Polynomial trend analyses indicated highly significant linear and quadratic response curves for percentage of tall fescue reduction for each herbicide. Fall and spring treatments with chlorsulfuron and diclofop provided significant tall fescue control, with slight to moderate initial Kentucky bluegrass phytotoxicity. Fall and spring applications of sulfometuron resulted in excellent tall fescue control, but initial Kentucky bluegrass damage was severe and would be unacceptable for high maintenance turf. Chemical names used: 2-chloro- N -[[(4-methoxy-6-methyl-l,3,5-triazin-2-yl)amino]carbonyl]-benzenesulfonamide (chlorsulfuron); 2-[4-(2,4-dichlorophenoxy)phenoxy]proponoate (diclofop); N -[[(4,6-dimethylpyrimidin-2-yl)amino]carbonyl]-2-methoxycarbonyl-benzenesulfonamide (sulfometuron).


2002 ◽  
Vol 12 (3) ◽  
pp. 465-469 ◽  
Author(s):  
D.S. Gardner ◽  
J.A. Taylor

In 1992, a cultivar trial was initiated in Columbus, Ohio to evaluate differences in establishment and long-term performance of cultivars of tall fescue (Festuca arundinacea), creeping red fescue (F. rubra), chewings fescue (F. rubra ssp. fallax), hard fescue (F. brevipila), kentucky bluegrass (Poa pratensis), rough bluegrass (P. trivialis), and perennial ryegrass (Lolium perenne) under low maintenance conditions in a shaded environment. Fertilizer and supplemental irrigation were applied until 1994 to establish the grasses, after which no supplemental irrigation, or pesticides were applied and fertilizer rates were reduced to 48.8 kg·ha-1 (1 lb/1000 ft2) of N per year. Percentage cover and overall quality data were collected in 2000 and compared with data collected in 1994. Initial establishment success does not appear to be a good predictor of long-term success of a cultivar in a shaded environment. There was some variability in cultivar performance under shade within a given turfgrass species. The tall fescue cultivars, as a group, had the highest overall quality and percentage cover under shade, followed by the fine fescues, kentucky bluegrass, rough bluegrass, and perennial ryegrass cultivars.


HortScience ◽  
2000 ◽  
Vol 35 (6) ◽  
pp. 1166-1169 ◽  
Author(s):  
Zachary J. Reicher ◽  
Clark S. Throssell ◽  
Daniel V. Weisenberger

Little documentation exists on the success of seeding cool-season turfgrasses in the late fall, winter and spring. The objectives of these two studies were to document the success of seeding Kentucky bluegrass (Poa pratensis L.), perennial ryegrass (Lolium perenne L.), and tall fescue (Festuca arundinacea Schreb.) at less-than-optimum times of the year, and to determine if N and P fertilizer requirements vary with seeding date of Kentucky bluegrass. `Ram I' Kentucky bluegrass, `Fiesta' perennial ryegrass, and `Mustang' tall fescue were seeded on 1 Sept., 1 Oct., 1 Nov., 1 Dec., 1 Mar., 1 Apr., and 1 May ± 2 days beginning in 1989 and 1990. As expected, the September seeding date produced the best establishment, regardless of species. Dormant-seeding Kentucky bluegrass and tall fescue in November, December, or March reduced the establishment time compared with seeding in April or May. Seeding perennial ryegrass in November, December, or March may not be justified because of winterkill potential. To determine the effect of starter fertilizer on seedings made at different times of the year, `Ram 1' Kentucky bluegrass was seeded 1 Sept., 1 Nov., 1 Mar., and 1 May ± 2 days in 1989 and 1990, and the seedbed was fertilized with all combinations of rates of N (0, 24, and 48 kg·ha-1) and P (0, 21, and 42 kg·ha-1). Fertilizer rate had no effect on establishment regardless of seeding date, possibly because of the fertile soil on the experimental site.


Author(s):  
Rahayu Rahayu ◽  
Yang Geun Mo ◽  
Choi Joon Soo

This research was column pot experiment with turfgrass was Kentucky bluegrass (<em>Poa pratensis</em>) plant irrigated saline irrigation and the column soaked in saline water. Rootzone profile consisted of 20 cm using saline lake dredged up sand. The sand amendments of the root zone were soil, zeolite, bottom ash, and peat. The mixtures of topsoil were; 90% sand + 10% peat moss, 80% sand + 10% soil + 10 % bottom ash, 80% sand + 20% soil, 90% sand + 5% peat + 5% zeolite, and 80% sand + 20% bottom ash. Interruption layer with coarse sand with diameters over 2 mm of 20 cm and 10 cm loamy soil as the bottom layer of the column. The result showed that Kentucky bluegrass could grow in sand based growing media amended by peat, sandy loam soils, bottom ash and zeolite being irrigated by 2 dS m<sup>-1</sup> saline water. Sand-based growing media amended by peat resulted in the highest clipping weigh but showed the highest salt accumulations. Sand amended by bottom ash and applied gypsum decreased clipping weigh, decreased SAR and increased calcium (Ca) when compared to the soil + peat (SP).  Sand amended by zeolite and gypsum decreased clipping weight, decreased sodium adsorption ratio (SAR) and higher Ca. Higher soil moisture retention of growing media promoted the growth of Kentucky bluegrass in spring, and lower moisture content promoted the growth in summer and fall season.


1973 ◽  
Vol 53 (1) ◽  
pp. 69-73 ◽  
Author(s):  
W. E. CORDUKES ◽  
A. J. MACLEAN

Addition of CaCl2∙2H2O at the rate of 2,000 ppm to give a conductivity of 7.2 mmhos/cm in a saturated paste extract of three soils varying in texture from sand to clay loam had no apparent effects on the quality of turf of Kentucky bluegrass, Poa pratensis L., creeping red fescue, Festuca rubra L., and perennial ryegrass, Lolium perenne L., grown in pot tests. When the rate was increased to 8,000 ppm and the conductivity to about 20 mmhos/cm, the turfgrass deteriorated markedly in the clay loam and the sandy loam and to a lesser degree in the sand. All species reacted similarly to the detrimental effect of salt in the soils, and the deterioration in turf quality was accompanied by a decline in root production. The concentration of chloride in leaf tissue usually increased with increasing amounts of salt in the soils and tended to be highest in perennial ryegrass and lowest in creeping red fescue. At the higher rates of salt addition, the amounts of chloride in the turfgrass tended to be lower on the sand than on the other soils.


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