scholarly journals Occurrence of Potyviruses on Yam (Dioscorea spp.) in Colombia and First Molecular Characterization of Yam mild mosaic virus

Plant Disease ◽  
2001 ◽  
Vol 85 (7) ◽  
pp. 803-803 ◽  
Author(s):  
S. Dallot ◽  
M. Guzmán ◽  
M. Bousalem
Keyword(s):  
Mosaic Virus ◽  
Protein Gene ◽  
Atlantic Coast ◽  
Enzyme Linked Immunosorbent Assay ◽  
Mild Mosaic ◽  
Coated Plate ◽  
Mild Mosaic Virus ◽  
The Caribbean ◽  
Polymerase Chain

A survey to determine the prevalence of potyviruses on yams, Dioscorea alata and D. cayenensis-rotundata, was undertaken in Colombia. Two hundred fifty leaf samples showing mottling symptoms were collected on the Atlantic coast and analyzed by antigen-coated plate enzyme-linked immunosorbent assay with universal potyvirus monoclonal antibodies (Agdia, Elkhart, IN). Potyviruses were detected in 70% (165/235) of the D. alata and in 66% (10/15) of the D. cayenensis-rotundata samples. The presence of Yam mild mosaic virus (YMMV) was indicated in some of these samples by immunocapture reverse-transcriptase polymerase chain reaction performed as previously reported (1). A 600-bp fragment that included the core and C-terminal region of the coat protein gene (CP) and the 3′ untranslated region (3′UTR) was amplified from a D. alata isolate using universal potyvirus primers (1), cloned, and sequenced (EMBL Acc. AJ311725). Comparison with the two previously published YMMV sequences revealed 96.1 and 97.4% identity for the deduced amino acid sequence in the CP region, 74.1 and 83.2% nucleotide identity in the 3′UTR for Papua New Guinea (AB022424 [2]) and Martinique (AJ250336) isolates, respectively. YMMV is known to be widespread on D. alata in Africa and the South Pacific and has been recently identified in the Caribbean (1). To our knowledge, this is the first report of its occurrence in Colombia. A study of its incidence and genetic diversity in South America has been undertaken. References: (1) M. Bousalem and S. Dallot. Plant Disease 84:200, 2000. (2) S. Fuji et al. Arch Virol. 144:1415, 1999.

scholarly journals First Report and Molecular Characterization of Yam mild mosaic virus in Dioscorea alata on the Island of Martinique

Plant Disease ◽  
2000 ◽  
Vol 84 (2) ◽  
pp. 200-200 ◽  
Author(s):  
M. Bousalem ◽  
S. Dallot
Keyword(s):  
Monoclonal Antibodies ◽  
Mosaic Virus ◽  
Papua New Guinea ◽  
New Guinea ◽  
Enzyme Linked Immunosorbent Assay ◽  
Dioscorea Alata ◽  
Mild Mosaic ◽  
First Report ◽  
Mild Mosaic Virus ◽  
The Caribbean

Naturally infected Dioscorea alata plants showing mild mosaic were collected in 1998 on the island of Martinique in the Caribbean. Isolates were first screened by double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) with monoclonal antibodies raised against Yam mosaic virus (YMV) and antigen-coated plate ELISA with universal potyvirus monoclonal antibodies (Agdia, Elkhart, IN). A positive reaction was obtained only with the universal potyvirus antiserum. Immunocapture reverse-transcriptase polymerase chain reaction was performed for specific detection of Yam mild mosaic virus (YMMV [3]) and YMV. A product with the predicted size of 249 bp was obtained with YMMV primers. YMMV is a recently recognized distinct potyvirus infecting D. alata in West Africa and the South Pacific (2–4). It was originally described as Yam virus I and is synonymous with Dioscorea alata virus (4). To characterize the YMMV Martinique isolate, total RNA was extracted, and universal potyvirus degenerate primers (1) were used to amplify a 700-bp fragment that included the core and C-terminal region of the coat protein (CP) and 3′ untranslated region (3′UTR). Sequence information generated (EMBL AJ250336) from the cloned fragment was compared with sequences of other yam potyviruses. Sequence comparisons of the partial CP (453 nt) showed a similarity of 94.6% (amino acids [aa]) with the YMMV isolate from Papua New Guinea (EMBL AB022424 [2]); 72.2% (aa) with the Japanese yam mosaic virus (JYMV) isolate (EMBL AB016500); and 67 to 73% (aa) with 27 YMV isolates. These sequences are most diverse in the 3′UTR, which showed a similarity of 72.8% with the YMMV Papua New Guinea isolate, 30% with the JYMV isolate, and 26% with the YMV isolates. These results confirm, as previously shown by S. Fuji et al. (2), that YMMV should be classified as a new potyvirus of yam. This is the first report of the natural occurrence of YMMV in the Caribbean. References: (1) Colinet et al. Phytopathology 84:65, 1994. (2) S. Fuji et al. Arch Virol. 144:1415, 1999. (3) R. A. Munford and S. E. Seal. J. Virol. Methods 69:73, 1997. (4) B. O. Odu et al. Ann. Appl. Biol. 134:65, 1999.

scholarly journals A New Tymovirus Isolated From Solanum quitoense: Characterization and Prevalence in Two Solanaceous Crops in Ecuador

Plant Disease ◽  
2019 ◽  
Vol 103 (9) ◽  
pp. 2246-2251 ◽  
Author(s):  
Juan F. Cornejo-Franco ◽  
Robert A. Alvarez-Quinto ◽  
Samuel Grinstead ◽  
Dimitre Mollov ◽  
Alexander V. Karasev ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Open Reading Frames ◽  
Yellow Mosaic Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Mild Mosaic ◽  
Fresh Market ◽  
Certification Programs ◽  
Mild Mosaic Virus ◽  
Polymerase Chain ◽  
Production Areas

Naranjilla (Solanum quitoense Lam.) and tamarillo (S. betaceum Cav.) are two important perennial solanaceous crops grown in Ecuador for the fresh market and juice production. Viruses infecting tamarillo and naranjilla are currently poorly studied, and no clean stock program exists in Ecuador. Here, we report a new virus, provisionally named as naranjilla mild mosaic virus (NarMMV) (genus Tymovirus, family Tymoviridae), isolated from naranjilla grown in an orchard in Pichincha Province, Ecuador. The complete genome of the virus consists of 6,348 nucleotides and encodes three open reading frames typical for members of the genus Tymovirus. Phylogenetically, Chiltepin yellow mosaic virus, Eggplant mosaic virus, and the recently characterized naranjilla chlorotic mosaic virus (NarCMV) were found to be the closest relatives of NarMMV. Unlike NarCMV, the new virus induced mild mosaic in naranjilla and more severe symptoms in tamarillo. Similar to NarCMV, NarMMV was unable to systemically infect potato. Virus surveys found NarMMV prevalent in naranjilla production areas of two provinces of Ecuador, especially where hybrid cultivars of naranjilla were cultivated. NarMMV was also found in field-grown tamarillo. The new virus cross-reacted with antibodies developed against NarCMV. Hence, this antibody will be useful for its field diagnosis using enzyme-linked immunosorbent assay or immunocapture reverse transcription polymerase chain reaction in future virus-free certification programs.

scholarly journals First Outbreak of Banana Streak Badnavirus Infection in Commercial Export Bananas in Costa Rica

Plant Disease ◽  
2000 ◽  
Vol 84 (10) ◽  
pp. 1152-1152 ◽  
Author(s):  
C. Pasberg-Gauhl ◽  
B. E. Lockhart ◽  
S. Duran
Keyword(s):  
Costa Rica ◽  
Mosaic Virus ◽  
Disease Incidence ◽  
Atlantic Coast ◽  
Enzyme Linked Immunosorbent Assay ◽  
Mild Mosaic ◽  
Mild Mosaic Virus ◽  
Leaf Necrosis ◽  
Internal Necrosis ◽  
Banana Streak Badnavirus

Banana streak badnavirus (BSV) is the most widely occurring virus in banana and plantain (1) but has not been reported to be a significant problem in commercial export bananas. In early 1999, the first severe outbreak of BSV infection in commercial export bananas (Musa AAA cv. Grand Nain) was recorded at Siquirres on the Atlantic coast of Costa Rica. Disease incidence in the plantation was 60% and symptoms included foliar chlorotic streaks, stunting of plants, splitting and internal necrosis of pseudostems and fruits, cigar leaf necrosis, and bunch emergence through the pseudostem. Diseased plants within a 0.8 ha area were eliminated to prevent possible further spread of the disease. The presence of BSV in diseased plants was confirmed by enzyme-linked immunosorbent assay and immunosorbent electron microscopy (1). Cucumber mosaic virus and Banana mild mosaic virus, which also occur in banana and plantain in Latin America (2), were not detected in the plants tested. Other recent accounts of BSV occurrence in commercial banana plantations in South America (our unpublished results) suggest that BSV occurrence in export bananas may be more significant than previously thought. References: (1) F. Gauhl et al. Int. J. Pest Management 45:167, 1991. (2) D. R. Jones, ed. 1999. Diseases of Banana, Abacá and Enset. CABI Publishing, Wallingford, U.K.

Characterization of Bean Mild Mosaic Virus: Particle Morphology, Composition and RNA Cell-Free Translation

Intervirology ◽  
1989 ◽  
Vol 30 (5) ◽  
pp. 285-293 ◽  
Author(s):  
A.V. Karasev ◽  
S.N. Chirkov ◽  
A.S. Kaftanova ◽  
N.A. Miroshnichenko ◽  
N.A. Surgucheva ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Virus Particle ◽  
Particle Morphology ◽  
Mild Mosaic ◽  
Free Translation ◽  
Mild Mosaic Virus

Cloning and sequence analysis of the coat protein gene of barley mild mosaic virus

1993 ◽  
Vol 27 (1) ◽  
pp. 79-89 ◽  
Author(s):  
I.J. Foulds ◽  
V.J. Lea ◽  
C. Sidebottom ◽  
C.M. James ◽  
R.E. Boulton ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Coat Protein ◽  
Mosaic Virus ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Mild Mosaic ◽  
Mild Mosaic Virus

Molecular Detection and Characterization of Chinese Yam Mild Mosaic Virus Isolates

2014 ◽  
Vol 163 (11-12) ◽  
pp. 1036-1040 ◽  
Author(s):  
Mingqiang Wang ◽  
Fan Li ◽  
Guohui Zhou ◽  
Pingxiu Lan ◽  
Donglin Xu ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Molecular Detection ◽  
Mild Mosaic ◽  
Chinese Yam ◽  
Mild Mosaic Virus ◽  
Virus Isolates

Molecular analysis of the capsid protein gene of a german isolate of barley mild mosaic virus

1993 ◽  
Vol 12 (4) ◽  
Author(s):  
U. Schlichter ◽  
A. Sohn ◽  
E. Peerenboom ◽  
J. Schell ◽  
H.-H. Steinbi�
Keyword(s):  
Mosaic Virus ◽  
Capsid Protein ◽  
Molecular Analysis ◽  
Protein Gene ◽  
Mild Mosaic ◽  
Capsid Protein Gene ◽  
Mild Mosaic Virus ◽  
German Isolate

scholarly journals First Report of Banana mild mosaic virus Isolated from Plantains (Musa AAB) in Colombia

Plant Disease ◽  
2003 ◽  
Vol 87 (9) ◽  
pp. 1150-1150 ◽  
Author(s):  
H. Reichel ◽  
A. K. Martínez ◽  
J. A. Arroyave ◽  
R. Sedano ◽  
F. J. Morales ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Mosaic Virus ◽  
Australian Isolate ◽  
Streak Virus ◽  
Mild Mosaic ◽  
Chain Reaction ◽  
First Report ◽  
Queensland Department ◽  
Mild Mosaic Virus ◽  
Polymerase Chain

Plantains (Musa AAB) are important sources of food and income for millions of people in Colombia and other developing countries. Colombia is the largest producer of plantains (2) and the third largest exporter of bananas in the world. In 2001, plants of ‘Dominico-Hartón’ plantain showing mild chlorotic streak symptoms were observed in northwestern Colombia. Electron microscopy of symptomatic tissue extracts revealed the presence of filamentous virus-like particles approximately 800 nm long. Immunocapture reverse-transcription polymerase chain reaction was performed to test for the presence of Banana mild mosaic virus (BanMMV) as described by J. E. Thomas (unpublished, Queensland Department of Primary Industries, Australia) and Sharman et al. (3). For polymerase chain reaction (PCR), the upstream primer No. 193 (5′-CAC TTA GGT TTG TGT GAT GT-3′) (designed in this study by using the computer Program DNAMAN Version 4.13) and the downstream primer Poty1 (5′-GGA TCC CGG GTT TTT TTT TTT TTT TTT V-3′) (1,3; J. E. Thomas, unpublished, Queensland Department of Primary Industries, Australia) were used. Amplification products of the expected size (approximately 900 bp) were obtained and sequenced after cloning in a pCR2.1 plasmid vector. Analyses of nucleic acid sequences using the international sequence databases and the BLAST program yielded nucleotide and amino acid sequence similarities of 80 to 83% and 90 to 92%, respectively, with an Australian isolate of BanMMV (GenBank Accession No. AF314662). The coat protein (CP) gene of the Colombian BanMMV isolate consists of 717 nucleotides. When the CP of the Colombian BanMMV isolates (GenBank Accession Nos. AY319331, AY319332, and AY319333) was compared with the CP of the Australian isolate, a highly variable region was observed in the N-terminus region. To our knowledge, this is the first report of BanMMV isolated from plantains in Colombia and the presence of molecular variability in the CP of BanMMV isolates. BanMMV has been found in Colombia associated with Banana streak virus and Cucumber mosaic virus in plantain. References: (1) A. Gibbs and A. Mackenzie. J. Virol.Methods 63:9, 1997 (2) N. S. Price. Infomusa 8(2):26, 1999. (3) M. Sharman et al. J. Virol. Methods 89:75, 2000.

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