Internal defect scanning of sweetpotatoes using interactance spectroscopy

While standard visible-light imaging offers a fast and inexpensive means of quality analysis of horticultural products, it is generally limited to measuring superficial (surface) defects. Using light at longer (near-infrared) or shorter (X-ray) wavelengths enables the detection of superficial tissue bruising and density defects, respectively; however, it does not enable the optical absorption and scattering properties of sub-dermal tissue to be quantified. This paper applies visible and near-infrared interactance spectroscopy to detect internal necrosis in sweetpotatoes and develops a Zemax scattering simulation that models the measured optical signatures for both healthy and necrotic tissue. This study demonstrates that interactance spectroscopy can detect the unique near-infrared optical signatures of necrotic tissues in sweetpotatoes down to a depth of approximately 5±0.5 mm. We anticipate that light scattering measurement methods will represent a significant improvement over the current destructive analysis methods used to assay for internal defects in sweetpotatoes.

Vine Removal Prior to Harvest, and Curing Duration and Temperature Affect the Incidence and Severity of Internal Necrosis in ‘Covington’ Sweetpotato

Internal necrosis (IN) is a physiological disorder that affects Covington, the most commonly grown sweetpotato (Ipomoea batatas) cultivar in North Carolina. Because IN affects the quality of sweetpotato storage roots, studies have been conducted since the first report of IN in 2006. Field studies (three in 2016 and two in 2017) were conducted to evaluate preharvest and postharvest treatments on the occurrence of IN in ‘Covington’ storage roots. Four preharvest treatments consisted of combinations of high chlorine or minimal chlorine potash fertilizer and mowing vs. not mowing before harvest. For postharvest treatments, 30 storage roots were obtained at harvest from each preharvest treatment plot and immediately cured in 75 and 85 °F rooms for a duration of 0.5, 1, 2, 3, and 5 weeks in 2016, and 0.5, 1, and 2 weeks in 2017. Shorter curing durations (0.5 and 1 week) coincided with industry recommendations while longer durations mimicked the challenges that some commercial facilities face when cooling down temperatures of rooms after curing is supposed to be concluded. Once curing temperature and curing duration treatments were completed, roots were placed in a 58 °F storage room at 85% relative humidity until cut. A control comparison was included in which harvested roots were placed in a 58 °F storage room (no curing) immediately after harvest. The storage roots from all temperature treatments were then cut 49 to 80 days after harvest, and incidence and severity of IN visually rated. Preharvest potash fertilizer treatments had minimal or no effect on occurrence of IN. However, mowing vines before harvest in several studies reduced IN incidence when roots were cured for more than 0.5 week at temperatures of at least 75 °F. Lower temperature (75 vs. 85 °F) and shorter curing duration (0.5 vs. 1, 2, 3, or 5 weeks) resulted in reduced IN occurrence in ‘Covington’ sweetpotato.

Pheochromocytoma crisis with cyclic fluctuation in blood pressure mimics acute coronary syndrome

Summary Pheochromocytoma crisis results from the sudden release of large quantities of catecholamines and leads to progressive multiple organ dysfunction. Here we report a case of pheochromocytoma crisis with symptoms associated with acute coronary syndrome (ACS) and severe fluctuations in blood pressure (BP). A 43-year-old Japanese man with hypertension (240/120 mmHg) visited a general hospital for chest pain. Echocardiogram showed ST segment depression and blood test demonstrated elevated troponin T. However, emergent coronary angiography revealed normal findings. CT showed a large adrenal mass on the left side, which was suspected as the cause of chest pain and BP elevation. After the patient was transported to our hospital, his BP was found to oscillate between 70 and 240 mmHg, and level of consciousness was decreased. After hospitalization, he had a further decrease in consciousness, a rise in body temperature, and a gradual increase in the interval between the upper and lower systolic BP. His systolic BP varied between 30 mmHg and 300 mmHg at the intervals of 20-30 min. After a multimodality therapy, including α-blocker and high dose fluid replacement, the fluctuation in his BP was gradually decreased and got stabilized after approximately 24 h. Approximately 3 weeks later, he underwent left adrenalectomy. This case showed that pheochromocytoma with internal necrosis might be misdiagnosed as ACS. Furthermore, in cases with a large adrenal tumor and severe elevation or fluctuations of BP, pheochromocytoma should be suspected and treated with α-blockers and fluid replacements as soon as possible prior to surgery. Learning points: High catecholamine levels due to pheochromocytoma crisis might cause symptoms associated with acute coronary syndrome. Adrenal tumor with internal necrosis and the elevation or fluctuations of blood pressure should be suspected to be pheochromocytoma. If pheochromocytoma crisis is suspected, the specialist, such as an endocrinologist or a urologist, should intervene, and an α-blocker treatment with adequate fluid replacement therapy should be initiated as soon as possible. Pheochromocytoma multisystem crisis (PMC) is a fatal condition characterized by multiple organ failure, severe blood pressure variability, high fever, and encephalopathy. This is an extremely rare subtype of a very rare disease such as pheochromocytoma. However, because the fatality rate of PMC is high, clinicians should be aware of the symptoms that mark its onset.

A PCR-Based Method for the Rapid Detection of Chondrostereum purpureum in Apple

Silverleaf caused by the basidiomycete Chondrostereum purpureum affects numerous woody species, including fruit tree crops like apple, resulting in wood necrosis and foliar silvering. There are no curative alternatives for this disease, and its management is by prevention methods. Therefore, the aim of this study was to develop a rapid diagnostic tool for the detection and identification of C. purpureum directly from woody tissues to help distinguish the pathogen from other basidiomycetes that are commonly found on apple. The silverleaf pathogen was isolated from different hosts and locations, and Koch’s postulates were performed by inoculating the isolates on apple cuttings and measuring internal necrosis. A previously described APN 1 pair of primers specificity was also tested against 25 C. purpureum isolates in this study, using other wood rotting species as negative controls. Seven virulent isolates were inoculated on apple cuttings, and DNA was extracted from the cuttings’ sawdust and amplified using APN 1, after 22 days of incubation. To prove the efficiency of the method in the field, DNA from healthy nursery plants inoculated with two virulent isolates, and naturally infected plants showing different levels of foliar symptoms, were tested. Presence of the fungus was verified by reisolation on APDA in all assays. Koch’s postulates indicated that all C. purpureum isolates were pathogenic, showing different virulence levels, and APN 1 primers were able to discriminate them from other basidiomycetes. The method was also able to detect C. purpureum from artificially inoculated plants as well as naturally infected ones, demonstrating that the protocol may become a rapid minimally destructive diagnostic tool to detect the pathogen without the need to isolate it from tissues, and thus taking measures to prevent its dissemination.

Potato Tuber Necrosis Induced by Alfalfa Mosaic Virus Depends on Potato Cultivar Rather Than on Virus Strain

Alfalfa mosaic virus (AMV) was identified as the causal agent of internal tuber necrosis in the potato cultivar Innovator in New Brunswick, Canada. Further pathological characterization of the isolate (designated as isolate CaM) was performed on six potato cultivars and one breeding clone. Upon mechanical inoculation, four cultivars (Innovator, Yukon Gold, Rochdale Gold-Dorée, and Shepody) showed needle-sized necrotic spots and increasing calico symptoms on new leaves, whereas the remaining cultivars only developed calico symptoms on new leaves. All tubers of CaM-infected Innovator and Shepody plants developed sporadic internal necrotic spots, as did ca. 23 and 8% tubers of CaM-infected Yukon Gold and Rochdale Gold-Dorée, respectively. Sequence analysis of the CP gene of CaM with AMV isolates from potato, all presumed belonging to the “non-necrotic” strain and retrieved from GenBank, indicated that CaM shared >97.1% sequence identity with all but four Egyptian isolates. At the complete genome level, phylogenetic analysis of all available sequences demonstrated that RNA 1 and RNA 3 can be grouped into three major clades each, whereas RNA 2 can be clustered into two clades. CaM and Ca175-1, an AMV isolate that was deemed non-necrotic in a previous study, had different phylogenetic clade patterns, indicating different RNA 1-RNA 2-RNA 3 haplotypes: IA-I-IB (CaM) versus Ca175-1 (IB-II-IA). Despite the difference in haplotype composition, CaM and Ca175-1 induced similar levels of internal necrosis in tubers of Innovator and its parent Shepody. The results suggest that the internal necrosis in AMV-infected tubers depends on potato cultivar rather than on AMV strain/haplotype, and CaM is just a “regular” isolate of AMV.

Effect of Cultivar, Ethephon, Flooding, and Storage Duration on Sweetpotato Internal Necrosis

The reason for internal necrosis occurrences in sweetpotato (Ipomoea batatas) storage roots is not well understood. This disorder begins internally in the storage roots as small light brown spots near the proximal end of the root that eventually can become more enlarged as brown/black regions in the cortex. The objective of this study was to determine the effect of ethephon and flooding on the development of internal necrosis in the sweetpotato cultivars Beauregard, Carolina Ruby, and Covington over storage durations from 9 to 150 days after harvest (DAH) when roots had been cured. Soil moisture treatments were no-flooding, and simulated flooding that was created by applying 10 inches of overhead irrigation during 2 weeks before harvest. Ethephon was applied at 0, 0.75, and 0.98 lb/acre 2 weeks before harvest. Overall, ‘Covington’ and ‘Carolina Ruby’ had greater internal necrosis incidence (22% to 65% and 32% to 51%, respectively) followed by ‘Beauregard’ (9% to 22%) during storage duration from 9 to 150 DAH at both soil moistures. No significant change was observed for either internal necrosis incidence or severity for ‘Beauregard’ and ‘Carolina Ruby’ over the storage duration of 9–150 DAH. However, there was an increase of internal necrosis incidence and severity 9–30 DAH in ‘Covington’, with incidence and severity remaining similar 30–150 DAH. Storage roots in treatments sprayed with 0.75 or 0.98 lb/acre ethephon had higher internal necrosis incidence and severity compared with the nontreated, regardless of cultivars at both soil moistures. This research confirms that sweetpotato cultivars differ in their susceptibility to internal necrosis (incidence and severity), ethephon applied to foliage can contribute to internal necrosis development in storage roots, and internal necrosis incidence reaches a maximum by 30 DAH in ‘Covington’ and 9 DAH in ‘Carolina Ruby’ and ‘Beauregard’.

Influence of Herbicides on the Development of Internal Necrosis of Sweetpotato

Field studies were conducted to determine the influence of herbicides on the development of internal necrosis (IN) in sweetpotato storage roots. In a slip propagation study, herbicide treatments included PRE application (immediately after covering seed roots with soil) of clomazone (0.42, 0.84 kg ai ha-1), flumioxazin (0.11, 0.21 kg ai ha-1), fomesafen (0.28, 0.56 kg ai ha-1), linuron (0.56, 1.12 kg ai ha-1),S-metolachlor (0.8, 1.6 kg ai ha-1), flumioxazin plusS-metolachlor (0.11 + 0.8 or 1.6 kg ha-1), and napropamide (1.12, 2.24 kg ai ha-1), and POST application (2 to 4 wk prior to cutting slips) of ethephon (0.84, 1.26 kg ai ha-1) and paraquat (0.14, 0.28 kg ai ha-1). In a field production study, flumioxazin, fomesafen, linuron, and paraquat were applied PREPLANT (one d prior to sweetpotato transplanting), clomazone,S-metolachlor, and napropamide were applied PRE [4 d after transplanting (DAP)], flumioxazin PREPLANT followed by (fb) S-metolachlor PRE, and ethephon applied POST (2 wk prior to harvest). Herbicide rates were similar to those used in the slip propagation study. Yield of sweetpotato in both studies was not affected by herbicide treatment. In both studies, IN incidence and severity increased with time and was greatest at 60 d after curing. No difference was observed between herbicide treatments for IN incidence and severity in the slip production study which indicates herbicide application at time of slip propagation does not impact the development of IN. In the field production study, the only treatment that increased IN incidence compared to the nontreated was ethephon with 53% and 2.3 incidence and severity, respectively. The presence of IN affected roots in nontreated plots indicates that some other pre- or post-curing factors other than herbicides are responsible for the development of IN. However, the ethephon application prior to sweetpotato root harvest escalates the development of IN.

Cryptosphaeria Dieback of Fremont Cottonwood Caused by Cryptosphaeria pullmanensis and C. multicontinentalis in California

Fremont cottonwood is a large and tall tree native to riparian areas in the southwestern United States. It grows along streams, rivers, and wetlands and serves many ecological and socioeconomic functions. In recent years, we observed a severe decline of Fremont cottonwood trees in California. Trees showed branches dying back, with dark-brown internal discoloration and decay of the wood of twigs, branches, or trunks. Eventually, the cambium and the bark were killed, causing a canker. The fungus Cryptosphaeria pullmanensis was isolated consistently from the necrotic wood of branches and twigs. On rare occasion, C. multicontinentalis was also isolated from symptomatic wood. Therefore, we investigated the pathogenicity in Fremont cottonwood of C. pullmanensis and C. multicontinentalis. Koch’s postulates were completed in saplings and both species appeared highly virulent, producing internal necrosis and staining of the wood. This study is the first to report Cryptosphaeria dieback of Populus fremontii caused by C. pullmanensis and C. multicontinentalis. Symptoms and signs of this new disease are described and illustrated.