scholarly journals Virus Coat Protein Transgenic Papaya Provides Practical Control of Papaya ringspot virus in Hawaii

Plant Disease ◽  
2002 ◽  
Vol 86 (2) ◽  
pp. 101-105 ◽  
Author(s):  
S. A. Ferreira ◽  
K. Y. Pitz ◽  
R. Manshardt ◽  
F. Zee ◽  
M. Fitch ◽  
...  
Keyword(s):  
Coat Protein ◽  
Transgenic Line ◽  
Field Trials ◽  
Fruit Production ◽  
Ringspot Virus ◽  
Soluble Solids ◽  
Production Data ◽  
Papaya Ringspot Virus ◽  
The Matrix ◽  
Virus Coat

Since 1992, Papaya ringspot virus (PRSV) destroyed nearly all of the papaya hectarage in the Puna district of Hawaii, where 95% of Hawaii's papayas are grown. Two field trials to evaluate transgenic resistance (TR) were established in Puna in October 1995. One trial included the following: SunUp, a newly named homozygous transformant of Sunset; Rainbow, a hybrid of SunUp, the nontransgenic Kapoho cultivar widely grown in Puna, and 63-1, another segregating transgenic line of Sunset. The second trial was a 0.4-ha block of Rainbow, simulating a near-commercial planting. Both trials were installed within a matrix of Sunrise, a PRSV-susceptible sibling line of Sunset. The matrix served to contain and trace pollen flow from TR plants, and as a secondary inoculum source. Virus infection was first observed 3.5 months after planting. At a year, 100% of the non-TR control and 91% of the matrix plants were infected, while PRSV infection was not observed on any of the TR plants. Fruit production data of SunUp and Rainbow show that yields were at least three times higher than the industry average, while maintaining percent soluble solids above the minimum of 11% required for commercial fruit. These data suggest that transgenic SunUp and Rainbow, homozygous and hemizygous for the coat protein transgene, respectively, offer a good solution to the PRSV problem in Hawaii.

Allergenicity Assessment of the Papaya Ringspot Virus Coat Protein Expressed in Transgenic Rainbow Papaya

2011 ◽  
Vol 59 (18) ◽  
pp. 10006-10012 ◽  
Author(s):  
Gustavo Fermín ◽  
Ronald C. Keith ◽  
Jon Y. Suzuki ◽  
Stephen A. Ferreira ◽  
Douglas A. Gaskill ◽  
...  
Keyword(s):  
Coat Protein ◽  
Ringspot Virus ◽  
Papaya Ringspot Virus ◽  
Virus Coat Protein ◽  
Virus Coat

scholarly journals Transformation of Plum with the Papaya Ringspot Virus Coat Protein Gene and Reaction of Transgenic Plants to Plum Pox Virus

1995 ◽  
Vol 120 (6) ◽  
pp. 943-952 ◽  
Author(s):  
Ralph Scorza ◽  
Laurene Levy ◽  
Vern Damsteegt ◽  
Luz Marcel Yepes ◽  
John Cordts ◽  
...  
Keyword(s):  
Coat Protein ◽  
Kanamycin Resistance ◽  
Ringspot Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Plum Pox Virus ◽  
Papaya Ringspot Virus ◽  
Indolebutyric Acid ◽  
Systemic Spread ◽  
Foreign Genes ◽  
Virus Coat

Transgenic plum plants expressing the papaya ringspot virus (PRV) coat protein (CP) were produced by Agrobacterium-mediated transformation of hypocotyl slices. Hypocotyl slices were cocultivated with Agrobacterium tumefaciens strain C58/Z707 containing the plasmid pGA482GG/CPPRV-4. This plasmid carries the PRVCP gene construct and chimeric NPTII and GUS genes. Shoots were regenerated on Murashige and Skoog salts, vitamins, 2% sucrose, 2.5 μm indolebutyric acid, 7.5 μm thidiazuron, and appropriate antibiotics for selection. Integration of the foreign genes was verified through kanamycin resistance, GUS assays, polymerase chain reaction (PCR), and Southern blot analyses. Four transgenic clones were identified. Three were vegetatively propagated and graft-inoculated with plum pox virus (PPV)-infected budwood in a quarantine, containment greenhouse. PPV infection was evaluated over a 2- to 4-year period through visual symptoms, enzyme-linked immunosorbent assay, and reverse transcriptase PCR assays. While most plants showed signs of infection and systemic spread of PPV within l-6 months, one plant appeared to delay the spread of virus and the appearance of disease symptoms. Virus spread was limited to basal portions of this plant up to 19 months postinoculation, but, after 32 months symptoms were evident and virus was detected throughout the plant. Our results suggest that heterologous protection with PRVCP, while having the potential to delay PPV symptoms and spread throughout plum plants, may not provide an adequate level of long-term resistance.

scholarly journals Field Evaluation of Transgenic Papaya Lines Carrying the Coat Protein Gene of Papaya ringspot virus in Taiwan

Plant Disease ◽  
2004 ◽  
Vol 88 (6) ◽  
pp. 594-599 ◽  
Author(s):  
Huey-Jiunn Bau ◽  
Ying-Huey Cheng ◽  
Tsong-Ann Yu ◽  
Jiu-Sherng Yang ◽  
Pan-Chi Liou ◽  
...  
Keyword(s):  
Coat Protein ◽  
Transgenic Plants ◽  
Rainy Season ◽  
Field Evaluation ◽  
Weather Conditions ◽  
Fruit Production ◽  
Ringspot Virus ◽  
Papaya Ringspot Virus ◽  
Transgenic Papaya ◽  
Transgenic Lines

Four transgenic papaya lines expressing the coat protein (CP) gene of Papaya ringspot virus (PRSV) were evaluated under field conditions for their reaction to PRSV infection and fruit production in 1996 to 1999. Plants were exposed to natural virus inoculation by aphids in two adjacent fields in four different plantings at the same sites. None of the transgenic lines showed severe symptoms of PRSV whereas control nontransgenic plants were 100% severely infected 3 to 5 months after planting. In the first and second trials, 20 to 30% of the transgenic plants showed mild symptoms consisting of confined mottling or chlorotic spots on leaves. The number of transgenic plants with mild symptoms fluctuated according to the season and weather conditions, with a tendency to increase in the winter or rainy season and decrease in the summer. Also, the incidence of the mild symptoms in the third trial increased significantly due to infection by root rot fungi during the rainy season. Interestingly, there was no apparent adverse effect on fruit yield and quality in transgenic plants with mild symptoms. In the first and second experiments, transgenic lines yielded 10.8 to 11.6 and 54.3 to 56.7 times more marketable fruit, respectively, than controls. All transgenic plants produced fruit of marketable quality with no ringspots or distortion.

scholarly journals Papaya Ringspot Virus Coat Protein Gene for Antigen Presentation in Escherichia coli

BMB Reports ◽  
2006 ◽  
Vol 39 (1) ◽  
pp. 16-21 ◽  
Author(s):  
Supawat Chatchen ◽  
Mila Juricek ◽  
Paloma Rueda ◽  
Sunee Kertbundit
Keyword(s):  
Escherichia Coli ◽  
Coat Protein ◽  
Antigen Presentation ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Ringspot Virus ◽  
Papaya Ringspot Virus ◽  
Virus Coat Protein ◽  
Virus Coat

scholarly journals Study of interaction between Papaya ringspot virus coat protein and infected Carica papaya proteins

2021 ◽  
Vol 16 (1) ◽  
pp. 474-480
Author(s):  
Wanwisa Siriwan ◽  
Sittiruk Roytrakul ◽  
Srimek Chowpongpang ◽  
Aroonothai Sawwa
Keyword(s):  
Coat Protein ◽  
Carica Papaya ◽  
Ringspot Virus ◽  
Papaya Ringspot Virus ◽  
Virus Coat Protein ◽  
Virus Coat

scholarly journals Engineered Resistance Against Papaya ringspot virus in Venezuelan Transgenic Papayas

Plant Disease ◽  
2004 ◽  
Vol 88 (5) ◽  
pp. 516-522 ◽  
Author(s):  
Gustavo Fermin ◽  
Valentina Inglessis ◽  
Cesar Garboza ◽  
Sairo Rangel ◽  
Manuel Dagert ◽  
...  
Keyword(s):  
Amino Acid ◽  
Agrobacterium Tumefaciens ◽  
Coat Protein ◽  
Amino Acid Sequence ◽  
Sequence Similarity ◽  
Ringspot Virus ◽  
Amino Acid Sequence Similarity ◽  
Papaya Ringspot Virus ◽  
Local Varieties ◽  
Cp Gene

Local varieties of papaya grown in the Andean foothills of Mérida, Venezuela, were transformed independently with the coat protein (CP) gene from two different geographical Papaya ringspot virus (PRSV) isolates, designated VE and LA, via Agrobacterium tumefaciens. The CP genes of both PRSV isolates show 92 and 96% nucleotide and amino acid sequence similarity, respectively. Four PRSV-resistant R0 plants were intercrossed or selfed, and the progenies were tested for resistance against the homologous isolates VE and LA, and the heterologous isolates HA (Hawaii) and TH (Thailand) in greenhouse conditions. Resistance was affected by sequence similarity between the transgenes and the challenge viruses: resistance values were higher for plants challenged with the homologous isolates (92 to 100% similarity) than with the Hawaiian (94% similarity) and, lastly, Thailand isolates (88 to 89% similarity). Our results show that PRSV CP gene effectively protects local varieties of papaya against homologous and heterologous isolates of PRSV.

The chemical subunit of tobacco ringspot virus coat protein

Virology ◽  
1979 ◽  
Vol 93 (2) ◽  
pp. 398-412 ◽  
Author(s):  
P.W.G. Chu ◽  
R.I.B. Francki
Keyword(s):  
Coat Protein ◽  
Ringspot Virus ◽  
Tobacco Ringspot Virus ◽  
Virus Coat Protein ◽  
Virus Coat
Sign in / Sign up

Export Citation Format

Share Document