A Selective Medium for the Recovery and Enumeration of Fomitopsis meliae, Causing Lemon Canker and Wood Rot

2021 ◽  
Author(s):  
Jiahuai Hu
Keyword(s):  
Pathogenic Fungi ◽  
Selective Medium ◽  
Optimal Concentration ◽  
Plant Tissues ◽  
Plant Pathogenic Fungi ◽  
Malt Extract ◽  
Thiophanate Methyl ◽  
Spore Trapping ◽  
Air Sample ◽  
Trapping Device

A selective medium (FMS medium) was developed for the isolation and quantification of Fomitopsis meliae, the causal agent of lemon canker and brown wood rot, from plants, soil, and air. The optimal concentration and combination of fungicides and antibiotics was evaluated to determine the most selective condition for growing F. meliae. The resultant composition of the medium (FMS) per litre (pH 3.5) was: 16 mg thiophanate-methyl, 8 mg dichloran, 5 mg 2-phenylphenol, 100 mg fluopyram, 0.5 mg fludioxonil, 100 mg chloramphenicol, 100 mg streptomycin, 15 g malt extract, 2.5 g mycological peptone, and 15 g agar. The fungus was successfully isolated and enumerated from air, soil and plant tissues using FMS medium. Furthermore, FMS medium almost completely inhibited the growth of other plant pathogenic fungi, soil and air saprophytes. This selectivity was high enough to estimate spore inoculum of F. meliae in an air sample or as a spore trapping device in commercial lemon orchards. FMS medium will be useful for studying epidemiology and management of F. meliae.

Fungal Phytotoxins in Sustainable Weed Management

2018 ◽  
Vol 25 (2) ◽  
pp. 268-286 ◽  
Author(s):  
Maurizio Vurro ◽  
Angela Boari ◽  
Francesca Casella ◽  
Maria Chiara Zonno
Keyword(s):  
Weed Management ◽  
Pathogenic Fungi ◽  
Mechanisms Of Action ◽  
Limiting Factors ◽  
Integrated Weed Management ◽  
Plant Pathogenic Fungi ◽  
Design Strategies ◽  
Disease Etiology ◽  
Chemical Structures ◽  
Toxic Metabolites

Fungal phytotoxins are natural secondary metabolites produced by plant pathogenic fungi during host–pathogen interactions. They have received considerable particular attention for elucidating disease etiology, and consequently to design strategies for disease control. Due to wide differences in their chemical structures, these toxic metabolites have different ecological and environmental roles and mechanisms of action. This review aims at summarizing the studies on the possible use of these metabolites as tools in biological and integrated weed management, e.g. as: novel and environmentally friendly herbicides; lead for novel compounds; sources of novel mechanisms of action. Moreover, the limiting factors for utilizing those metabolites in practice will also be briefly discussed.

First record of Pestalotiopsis spp. from affected leaves of mastic shrubs (Pestacia lentiscus L.) in northeastern of Libya.

2016 ◽  
Vol 5 (08) ◽  
pp. 4744
Author(s):  
Zahra Ibrahim El-Gali
Keyword(s):  
Signs And Symptoms ◽  
Pathogenic Fungi ◽  
First Record ◽  
Plant Pathogenic Fungi ◽  
Pistacia Lentiscus ◽  
Plant Materials ◽  
Al Jabal Al Akhdar ◽  
First Time

This study was carried out to identify the unknown different symptoms and their causes as plant pathogenic fungi from Al-Jabal Al-Akhdar District. Plant materials with fungal signs and symptoms were collected and examined. The main fungi consistently isolated from symptomatic leaves and twigs were Pestalotiopsis spp. Morphology, colony characteristics, and pathogenicity of the isolates were examined. My report the occurrence of Pestalotiopsis spp. on leaves of mastic (Pistacia lentiscus) for the first time in Libya.

scholarly journals Functional analysis of the conserved transcriptional regulator CfWor1 inCladosporium fulvumreveals diverse roles in the virulence of plant pathogenic fungi

2014 ◽  
Vol 92 (1) ◽  
pp. 10-27 ◽  
Author(s):  
Bilal Ökmen ◽  
Jérôme Collemare ◽  
Scott Griffiths ◽  
Ate van der Burgt ◽  
Russell Cox ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Transcriptional Regulator ◽  
Pathogenic Fungi ◽  
Plant Pathogenic Fungi

Antifungal activity of phthalide lactones on plant pathogenic fungi and yeast

2018 ◽  
Vol 280 ◽  
pp. S27
Author(s):  
Enriqueta Martinez Rojas ◽  
Fatuma Hassan ◽  
Filip Boratynski ◽  
Teresa Olejniczak ◽  
Karl Steffens ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Pathogenic Fungi ◽  
Plant Pathogenic Fungi

Dynamics of fungal pathogens in host plant tissues

1995 ◽  
Vol 73 (S1) ◽  
pp. 1275-1283 ◽  
Author(s):  
Shigehito Takenaka
Keyword(s):  
Host Plant ◽  
Species Interactions ◽  
Fungal Biomass ◽  
Quantitative Methods ◽  
Plant Pathogens ◽  
Pathogenic Fungi ◽  
Molecular Methods ◽  
Plant Colonization ◽  
Fungal Colonization ◽  
Plant Tissues

To develop efficient control measures against fungal plant pathogens, the dynamics of host plant colonization during disease development and the interactions among fungi within host plant tissues need to be clarified. These studies require accurate quantitative estimation of specific fungal biomass in plant tissues. This has been approached by direct-microscopic methods, cultural methods, chemical determinations of fungal components, serological methods, and molecular methods. Among these methods, serological and molecular methods provide rapid, specific, and sensitive quantitative measures of fungal biomass in host plant tissues. Therefore, studies on fungal dynamics of host plant colonization using these two methods are presented. Some examples of species interactions among pathogenic fungi within host plants, such as synergism and competition, are reviewed and the usefulness of serological and molecular methods for studies on these interactions is presented. These quantitative methods will provide helpful information for understanding the ecology of plant pathogenic fungi, such as the dynamics of host plant colonization and species interactions. Key words: quantitative methods, fungal biomass, ELISA, PCR, fungal colonization, species interaction.

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