A Selective Medium for the Recovery and Enumeration of Fomitopsis meliae, Causing Lemon Canker and Wood Rot

A selective medium (FMS medium) was developed for the isolation and quantification of Fomitopsis meliae, the causal agent of lemon canker and brown wood rot, from plants, soil, and air. The optimal concentration and combination of fungicides and antibiotics was evaluated to determine the most selective condition for growing F. meliae. The resultant composition of the medium (FMS) per litre (pH 3.5) was: 16 mg thiophanate-methyl, 8 mg dichloran, 5 mg 2-phenylphenol, 100 mg fluopyram, 0.5 mg fludioxonil, 100 mg chloramphenicol, 100 mg streptomycin, 15 g malt extract, 2.5 g mycological peptone, and 15 g agar. The fungus was successfully isolated and enumerated from air, soil and plant tissues using FMS medium. Furthermore, FMS medium almost completely inhibited the growth of other plant pathogenic fungi, soil and air saprophytes. This selectivity was high enough to estimate spore inoculum of F. meliae in an air sample or as a spore trapping device in commercial lemon orchards. FMS medium will be useful for studying epidemiology and management of F. meliae.

Surface-modified elastomeric nanofluidic devices for single nanoparticle trapping

Our work focuses on the development of simpler and effective production of nanofluidic devices for high-throughput charged single nanoparticle trapping in an aqueous environment. Single nanoparticle confinement using electrostatic trapping has been an effective approach to study the fundamental properties of charged molecules under a controlled aqueous environment. Conventionally, geometry-induced electrostatic trapping devices are fabricated using SiOx-based substrates and comprise nanochannels imbedded with nanoindentations such as nanopockets, nanoslits and nanogrids. These geometry-induced electrostatic trapping devices can only trap negatively charged particles, and therefore, to trap positively charged particles, modification of the device surface is required. However, the surface modification process of a nanofluidic device is cumbersome and time consuming. Therefore, here, we present a novel approach for the development of surface-modified geometry-induced electrostatic trapping devices that reduces the surface modification time from nearly 5 days to just a few hours. We utilized polydimethylsiloxane for the development of a surface-modified geometry-induced electrostatic trapping device. To demonstrate the device efficiency and success of the surface modification procedure, a comparison study between a PDMS-based geometry-induced electrostatic trapping device and the surface-modified polydimethylsiloxane-based device was performed. The device surface was modified with two layers of polyelectrolytes (1: poly(ethyleneimine) and 2: poly(styrenesulfonate)), which led to an overall negatively charged surface. Our experiments revealed the presence of a homogeneous surface charge density inside the fluidic devices and equivalent trapping strengths for the surface-modified and native polydimethylsiloxane-based geometry-induced electrostatic trapping devices. This work paves the way towards broader use of geometry-induced electrostatic trapping devices in the fields of biosensing, disease diagnosis, molecular analysis, fluid quality control and pathogen detection.

Perancangan Sistem Perangkap Hama Tanaman Petani Otomatis Menggunakan Modul Mikrokontroler Arduino

An animal can also be called a pest if it causes damage to natural ecosystems or acts as an agent for spreading disease in human habitats. The pest in question is a mouse-like animal that aims to make a trap using a load cell weight sensor with an Arduino microcontroller module. The scope of the problem in this research is the cultivation of maize and paddy fields in Mardingding District, Karo Regency. For testing purposes, 3 trials are used, respectively, corn and rice fields. The variable is limited to animals the size of a mouse. The purpose of this research is to build a plant pest trapping device using the Arduino uno micro controller module which can make it easier to catch pests efficiently. Farmers no longer need to control within a certain period of time. so it can save time and energy to do other jobs. The application is built using the programming language used is the C language and the Arduino IDE with notification to farmers only via short messages via cellphones.

A multi-location peak parking approach for calculation of second dimensional retention indices for improved volatile compound identification with cryogen-free comprehensive heart-cut two-dimensional gas chromatography

Comprehensive heart-cut multidimensional gas chromatography (CH/C MDGC) without a cryogenic trapping device was developed with an approach for calculation of first and second dimensional retention indices (1I and 2I).

Development and implementation of device for capturing large mechanical particles falling into the space between cylinder and plunger of sucker-rod pump

The deep pump, mainly sucker-rod deep pumping method is widely applied in the onshore oil wells of Azerbaijan. Despite the fact that the deep-well sucker rod pumping method is widespread, its operation in sand-producing wells is accompanied by various complications. Since mechanical particles wearing the pump parts out, falling into the space between the plunger and the cylinder, either wedge the plunger inside the cylinder or increase the gap between them. To prevent the ingress of large particles of sand into the gap between the plunger and the cylinder of the deep sucker rod pump, a device for sand trapping has been developed. The sand-trapping device is installed within the well tubing strings on the rod string and prevents the big particles of the liquid in the pipes falling into the gap between the plunger and cylinder. As the plunger, the device is started via balanced head. Due to the equipment installation, the liquid injected from the pump undergoes pressure decrease and the conditions for deposition and accumulation of large particles are formed.

The Mostela: an adjusted camera trapping device as a promising non-invasive tool to study and monitor small mustelids

In spite of their potential important role in shaping small mammal population dynamics, weasel (Mustela nivalis) and stoat (Mustela erminea) are understudied due to the difficulty of detecting these species. Furthermore, their conservation status in many countries is unknown due to lack of monitoring techniques. There is thus an important need for a method to detect these small mustelids. In this study, we tested the efficiency of a recently developed camera trapping device, the Mostela, as a new technique to detect mustelids in a study area near Dieren, the Netherlands. We placed Mostelas in linear landscape features, and other microhabitats thought to be frequently visited by weasels, from March to October 2017 and February to October 2018. We tested for yearly and monthly differences in site use and detectability, as well as the effect of entrance tube size, using an occupancy modelling framework. We found large seasonal differences in site use and detectability of weasels with the highest site use in June to October and highest detection probability in August and September. Detection probability was approximately two times higher for Mostelas with a 10-cm entrance tube compared with 8-cm. Furthermore, we were able to estimate activity patterns based on the time of detection, identify the sex in most detections (69.5%), and distinguish several individuals. Concluding, the Mostela seems promising as a non-invasive monitoring tool to study the occurrence and ecology of small mustelids. Further development of individual recognition from images would enable using the Mostela for density estimates applying capture-recapture models.