Characterization of a Severe Virus-like Disease in Chardonnay Grapevines in Missouri

2007 ◽  
Vol 8 (1) ◽  
pp. 39 ◽  
Author(s):  
Wenping Qiu ◽  
John D. Avery ◽  
Shaista Lunden
Keyword(s):  
Polymerase Chain Reaction ◽  
Reverse Transcription ◽  
Grapevine Fanleaf Virus ◽  
Chain Reaction ◽  
Cabernet Franc ◽  
Vein Clearing ◽  
Polymerase Chain

A severe virus-like disease emerged on Chardonnay vines in a Missouri vineyard that resulted in significant reduction of vine vigor and yield. The pathogens can be transmitted via cutting and grafting. Chardonnay, Cabernet franc and Baco franc vines that were grafted with the buds of the original diseased Chardonnay vines exhibited distinct vein-clearing symptom. The result of reverse transcription polymerase chain reaction indicated the association of Grapevine fanleaf virus with this disease. Accepted for publication 26 September 2007. Published 19 November 2007.

scholarly journals A Comparison Between Serological and Biological Assays in Detecting Grapevine Leafroll Associated Viruses

Plant Disease ◽  
1997 ◽  
Vol 81 (7) ◽  
pp. 799-801 ◽  
Author(s):  
Adib Rowhani ◽  
Jerry K. Uyemoto ◽  
Deborah A. Golino
Keyword(s):  
Polymerase Chain Reaction ◽  
Reverse Transcription ◽  
Multiple Infections ◽  
Enzyme Linked Immunosorbent Assay ◽  
Vitis Vinifera L ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Biological Assays ◽  
Cabernet Franc ◽  
Polymerase Chain

The efficacy of the serological procedure enzyme-linked immunosorbent assay (ELISA) for detecting grapevine leafroll associated viruses (GLRaV types -1, -2, -3, and -4) was compared with indexing on Vitis vinifera L. cv. Cabernet Franc. Results of the biological assays confirmed the infectious nature of all grapevine sources testing positive by ELISA for GLRaV-1 (9 sources), GLRaV-2 (14 sources), and GLRaV-4 (14 sources), and the noninfectious nature of ELISA-negative grapevines (75 sources). However, among 57 sources testing positive by ELISA for GLRaV-3, or 24 sources with multiple infections, 8 and 1 sources, respectively, were negative by Cabernet Franc assays. Serological assays were repeated on all graft-inoculated indicators and only symptomatic ones reacted positively. Also, the 8 original GLRaV-3 sources that had tested positive by ELISA and negative by bioassay were found positive using immuno-capture/reverse transcription-polymerase chain reaction (IC/RT-PCR). The single multiple-infected source was not available for retesting. The distribution of GLRaV in infected grapevines was tested by assaying 20 to 40 samples per source of 36 plants infected with GLRaV-1, -2, -3, or -4. The incidence of GLRaV-positive canes as determined by ELISA ranged from 0 to 100%, suggesting that GLRaV can be unevenly distributed in chronically infected grapevines.

1504: Molecular Diagnosis and Staging of Prostate Cancer Using Clusterin in Real Time Reverse Transcription Polymerase Chain Reaction (RT-PCR)

2006 ◽  
Vol 175 (4S) ◽  
pp. 485-486
Author(s):  
Sabarinath B. Nair ◽  
Christodoulos Pipinikas ◽  
Roger Kirby ◽  
Nick Carter ◽  
Christiane Fenske
Keyword(s):  
Prostate Cancer ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Molecular Diagnosis ◽  
Reverse Transcription ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Polymerase Chain
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