scholarly journals Dynamic Transcriptional Profiles of Arabidopsis thaliana Infected by Tomato spotted wilt virus

2020 ◽  
Vol 110 (1) ◽  
pp. 153-163 ◽  
Author(s):  
Min Xu ◽  
Jing Chen ◽  
Ying Huang ◽  
Danyu Shen ◽  
Peng Sun ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Regulatory Networks ◽  
Tomato Spotted Wilt Virus ◽  
Rna Virus ◽  
Economic Loss ◽  
Immune Defense ◽  
Tomato Spotted Wilt ◽  
Deep Sequencing Technology ◽  
Photosynthesis Pathway ◽  
Wilt Virus

Tomato spotted wilt virus (TSWV) is a negative-stranded RNA virus that infects hundreds of plant species, causing great economic loss. Infected Arabidopsis thaliana plants develop symptoms including chlorosis and wilt, which can lead to cell death. From 9 to 15 days after TSWV infection, symptoms progress through a three-stage process of appearance, severity, and death. In this study, deep sequencing technology was first used to explore gene expression in response to TSWV infection in model plant A. thaliana at different symptom development stages. We found that plant immune defense and protein degradation are induced by TSWV infection and that both inductions became stronger over time. The photosynthesis pathway was attenuated with TSWV infection. Cell wall metabolism had a large extent of downregulation while some genes were upregulated. These results illustrate the dynamic nature of TSWV infection in A. thaliana at the whole-transcriptome level. The link between biological processes and subpathway metabolism was further analyzed. Our study provides new insight into host regulatory networks and dynamic processes in response to TSWV infection.

Engineered Resistance to Tomato Spotted Wilt Virus, a Negative-Strand RNA Virus

1993 ◽  
pp. 386-386
Author(s):  
Jan J. L. Gielen ◽  
Peter de Haan ◽  
Mart Q. J. M. van Grinsven ◽  
Rob Goldbach ◽  
André W. Schram
Keyword(s):  
Tomato Spotted Wilt Virus ◽  
Rna Virus ◽  
Negative Strand ◽  
Tomato Spotted Wilt ◽  
Engineered Resistance ◽  
Wilt Virus

scholarly journals Developmentally regulated Arabidopsis thaliana susceptibility to tomato spotted wilt virus infection

2020 ◽  
Vol 21 (7) ◽  
pp. 985-998 ◽  
Author(s):  
Ying Huang ◽  
Hao Hong ◽  
Min Xu ◽  
Jiaoling Yan ◽  
Jing Dai ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Virus Infection ◽  
Tomato Spotted Wilt Virus ◽  
Developmentally Regulated ◽  
Tomato Spotted Wilt ◽  
Wilt Virus

scholarly journals The Induction of an Effective dsRNA-Mediated Resistance Against Tomato Spotted Wilt Virus by Exogenous Application of Double-Stranded RNA Largely Depends on the Selection of the Viral RNA Target Region

2020 ◽  
Vol 11 ◽  
Author(s):  
Saeid Tabein ◽  
Marco Jansen ◽  
Emanuela Noris ◽  
Anna Maria Vaira ◽  
Daniele Marian ◽  
...  
Keyword(s):  
Tomato Spotted Wilt Virus ◽  
Rna Viruses ◽  
Rna Virus ◽  
Promising Alternative ◽  
Exogenous Application ◽  
Tomato Spotted Wilt ◽  
Protection Efficacy ◽  
Systemic Symptoms ◽  
Wilt Virus ◽  
Negative Sense

Tomato spotted wilt virus (TSWV) is a devastating plant pathogen, causing huge crop losses worldwide. Unfortunately, due to its wide host range and emergence of resistance breaking strains, its management is challenging. Up to now, resistance to TSWV infection based on RNA interference (RNAi) has been achieved only in transgenic plants expressing parts of the viral genome or artificial microRNAs targeting it. Exogenous application of double-stranded RNAs (dsRNAs) for inducing virus resistance in plants, namely RNAi-based vaccination, represents an attractive and promising alternative, already shown to be effective against different positive-sense RNA viruses and viroids. In the present study, the protection efficacy of exogenous application of dsRNAs targeting the nucleocapsid (N) or the movement protein (NSm) coding genes of the negative-sense RNA virus TSWV was evaluated in Nicotiana benthamiana as model plant and in tomato as economically important crop. Most of the plants treated with N-targeting dsRNAs, but not with NSm-targeting dsRNAs, remained asymptomatic until 40 (N. benthamiana) and 63 (tomato) dpi, while the remaining ones showed a significant delay in systemic symptoms appearance. The different efficacy of N- and NSm-targeting dsRNAs in protecting plants is discussed in the light of their processing, mobility and biological role. These results indicate that the RNAi-based vaccination is effective also against negative-sense RNA viruses but emphasize that the choice of the target viral sequence in designing RNAi-based vaccines is crucial for its success.

scholarly journals Molecular Co-Chaperone SGT1 Is Critical for Cell-to-Cell Movement and Systemic Infection of Tomato Spotted Wilt Virus in Nicotiana benthamiana

Viruses ◽  
2018 ◽  
Vol 10 (11) ◽  
pp. 647 ◽  
Author(s):  
Xin Qian ◽  
Qing Xiang ◽  
Tongqing Yang ◽  
Hongyu Ma ◽  
Xin Ding ◽  
...  
Keyword(s):  
Virus Infection ◽  
Nicotiana Benthamiana ◽  
Tomato Spotted Wilt Virus ◽  
Rna Virus ◽  
Cell Movement ◽  
Systemic Infection ◽  
Plant Viruses ◽  
Host Factors ◽  
Tomato Spotted Wilt ◽  
Wilt Virus

Tospovirus is a tripartite negative stranded RNA virus and is considered as one of the most devastating plant viruses. Successful virus infection in plant requires many host factors. To date, very few host factors have been identified as important in Tospovirus infection in plants. We reported earlier that NSm protein encoded by Tomato spotted wilt virus (TSWV), a type species of the genus Orthotospovirus, plays critical roles in viral cell-to-cell and long-distance movement. In this study, we determined that molecular co-chaperone NbSGT1 interacted with TSWV NSm in Nicotiana benthamiana. TSWV infection significantly upregulated the expression of NbSGT1 gene and transient overexpression of NbSGT1 in N. benthamiana leaves accelerated TSWV infection. In contrast, silencing the NbSGT1 gene expression using a virus-induced gene silencing (VIGS) approach strongly inhibited TSWV NSm cell-to-cell movement, as well as TSWV local and systemic infection in N. benthamiana plants. Furthermore, NbSGT1 was found to regulate the infection of both American and Euro/Asia type tospoviruses in N. benthamiana plant. Collectively, our findings presented in this paper and the results published previously indicated that molecular co-chaperone NbSGT1 plays important roles in modulating both positive stranded and tripartite negative stranded RNA virus infection in plants.

Engineered Resistance to Tomato Spotted Wilt Virus, a Negative–Strand RNA Virus

1991 ◽  
Vol 9 (12) ◽  
pp. 1363-1367 ◽  
Author(s):  
Jan J. L. Gielen ◽  
Peter de Haan ◽  
Ad J. Kool ◽  
Dick Peters ◽  
Mart Q. J. M. van Grinsven ◽  
...  
Keyword(s):  
Tomato Spotted Wilt Virus ◽  
Rna Virus ◽  
Negative Strand ◽  
Tomato Spotted Wilt ◽  
Engineered Resistance ◽  
Wilt Virus

scholarly journals Current Status and Potential of RNA Interference for the Management of Tomato Spotted Wilt Virus and Thrips Vectors

Pathogens ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 320
Author(s):  
Alexander Nilon ◽  
Karl Robinson ◽  
Hanu R. Pappu ◽  
Neena Mitter
Keyword(s):  
Rna Interference ◽  
Tomato Spotted Wilt Virus ◽  
Cultural Practices ◽  
Management Strategies ◽  
Current Status ◽  
Horticultural Crops ◽  
Yield And Quality ◽  
Tomato Spotted Wilt ◽  
Wilt Virus

Tomato spotted wilt virus (TSWV) is the type member of the genus Orthotospovirus in the family Tospoviridae and order Bunyavirales. TSWV, transmitted by several species of thrips, causes significant disease losses to agronomic and horticultural crops worldwide, impacting both the yield and quality of the produce. Management strategies include growing virus-resistant cultivars, cultural practices, and managing thrips vectors through pesticide application. However, numerous studies have reported that TSWV isolates can overcome host-plant resistance, while thrips are developing resistance to pesticides that were once effective. RNA interference (RNAi) offers a means of host defence by using double-stranded (ds) RNA to initiate gene silencing against invading viruses. However, adoption of this approach requires production and use of transgenic plants and thus limits the practical application of RNAi against TSWV and other viruses. To fully utilize the potential of RNAi for virus management at the field level, new and novel approaches are needed. In this review, we summarize RNAi and highlight the potential of topical or exogenous application of RNAi triggers for managing TSWV and thrips vectors.

Lipid in a Purified Preparation of Tomato Spotted Wilt Virus

Nature ◽  
1964 ◽  
Vol 203 (4945) ◽  
pp. 671-672 ◽  
Author(s):  
RUPERT J. BEST ◽  
GERARD F. KATEKAR
Keyword(s):  
Tomato Spotted Wilt Virus ◽  
Tomato Spotted Wilt ◽  
Wilt Virus
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