scholarly journals Human milk exosomes dampen induced inflammatory response in human intestinal epithelial cells

2018 ◽  
Vol 32 (S1) ◽  
Author(s):  
Jamie Dale Kraft ◽  
Emanuela Ferretti ◽  
Eric Tremblay ◽  
Jean‐François Beaulieu ◽  
Illimar Altosaar
Keyword(s):  
Epithelial Cells ◽  
Inflammatory Response ◽  
Human Milk ◽  
Intestinal Epithelial Cells ◽  
Intestinal Epithelial ◽  
Human Intestinal Epithelial Cells

scholarly journals Vibrio cholerae Cytolysin Causes an Inflammatory Response in Human Intestinal Epithelial Cells That Is Modulated by the PrtV Protease

PLoS ONE ◽  
2009 ◽  
Vol 4 (11) ◽  
pp. e7806 ◽  
Author(s):  
Gangwei Ou ◽  
Pramod Kumar Rompikuntal ◽  
Aziz Bitar ◽  
Barbro Lindmark ◽  
Karolis Vaitkevicius ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Inflammatory Response ◽  
Vibrio Cholerae ◽  
Intestinal Epithelial Cells ◽  
Intestinal Epithelial ◽  
Human Intestinal Epithelial Cells

Melatonin reduces inflammatory response in human intestinal epithelial cells stimulated by interleukin‐1β

2019 ◽  
Vol 67 (3) ◽  
Author(s):  
Giuseppe Mannino ◽  
Fabio Caradonna ◽  
Ilenia Cruciata ◽  
Antonino Lauria ◽  
Anna Perrone ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Inflammatory Response ◽  
Intestinal Epithelial Cells ◽  
Interleukin 1Β ◽  
Intestinal Epithelial ◽  
Human Intestinal Epithelial Cells

Bcl10 mediates LPS-induced activation of NF-κB and IL-8 in human intestinal epithelial cells

2007 ◽  
Vol 293 (2) ◽  
pp. G429-G437 ◽  
Author(s):  
Sumit Bhattacharyya ◽  
Alip Borthakur ◽  
Nitika Pant ◽  
Pradeep K. Dudeja ◽  
Joanne K. Tobacman
Keyword(s):  
Epithelial Cells ◽  
Confocal Microscopy ◽  
Inflammatory Response ◽  
Western Blot ◽  
Intestinal Epithelial Cells ◽  
Ex Vivo ◽  
Intestinal Epithelial ◽  
Human Intestinal Epithelial Cells ◽  
Normal Human ◽  
Interfering Rna

Lipopolysaccharide (LPS) is recognized as an inducer of the inflammatory response associated with gram-negative sepsis and systemic inflammatory response syndrome. LPS induction proceeds through Toll-like receptor (TLR) in immune cells and intestinal epithelial cells (IEC). This report presents the first identification of Bcl10 (B-cell CLL/lymphoma 10) as a mediator of the LPS-induced activation of IL-8 in human IEC. Bcl10 is a caspase-recruitment domain-containing protein, associated with constitutive activation of NF-κB in MALT (mucosa-associated lymphoid tissue) lymphomas. The normal human IEC line NCM460, normal primary human colonocytes, and ex vivo human colonic tissue were exposed to 10 ng/ml of LPS for 2–6 h. Effects on Bcl10, phospho-IκBα, NF-κB, and IL-8 were determined by Western blot, ELISA, immunohistochemistry, and confocal microscopy. Effects of Bcl10 silencing by small-interfering RNA (siRNA), TLR4 blocking antibody, TLR4 silencing by siRNA, and an IL-1 receptor-associated kinase (IRAK)-1/4 inhibitor on LPS-induced activation were examined. Following Bcl10 silencing, LPS-induced increases in NF-κB, IκBα, and IL-8 were significantly reduced ( P < 0.001). Increasing concentrations of LPS were associated with higher concentrations of Bcl10 protein when quantified by ELISA, and the association between LPS exposure and increased Bcl10 was also demonstrated by Western blot, immunohistochemistry, and confocal microscopy. Exposure to TLR4 antibody, TLR4 siRNA, or an IRAK-1/4 inhibitor eliminated the LPS-induced increases in Bcl10, NF-κB, and IL-8. Identification of Bcl10 as a mediator of LPS-induced activation of NF-κB and IL-8 in normal human IEC provides new insight into mechanisms of epithelial inflammation and new opportunities for therapeutic intervention.

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