Role of Histone Deacetylases 5 (HDAC5) in Ang II‐Induced the Early Growth Response Protein‐1 (Egr‐1) Expression and Vascular Hypertrophy

2020 ◽  
Vol 34 (S1) ◽  
pp. 1-1 ◽  
Author(s):  
Vanessa Truong ◽  
Ashish Jain ◽  
Madhu Anand-Srivastava ◽  
Ashok Srivastava
Keyword(s):  
Histone Deacetylases ◽  
Growth Response ◽  
Early Growth ◽  
Ang Ii ◽  
Vascular Hypertrophy ◽  
Early Growth Response

The Role of Early Growth Response 1 in the Prognostic Value and Cell Migration of Breast Cancer

2021 ◽  
Author(s):  
Leiyu Hao ◽  
Fengru Huang ◽  
Xinqian Yu ◽  
Bujie Xu ◽  
Yan Liu ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Migration ◽  
Protein Expression ◽  
Prognostic Value ◽  
Growth Response ◽  
Expression Patterns ◽  
Early Growth ◽  
The Cancer Genome Atlas ◽  
Early Growth Response

Abstract Background: Early growth response family members (EGRs), EGR1-4, have increasingly attracted attention in multiple cancers. However, the exact expression patterns and prognostic values of EGRs in the progress of breast cancer (BRCA) remain largely unknown. Methods: The mRNA expression and prognostic characteristics of EGRs were examined by the Cancer Genome Atlas (TCGA), Oncomine and Kaplan-Meier plotter. Enrichment analyses were conducted based on protein-protein interaction (PPI) network. The Tumor Immune Estimation Resource (TIMER) database and MethSurv were further explored. The protein expression level of EGR1 and cell migration were measured by Western blotting, immunohistochemistry, wound-healing assay and Boyden chamber assay in BRCA. Results: The transcriptional levels of EGR1/2/3 displayed significantly low expression in BRCA compared to that in normal tissues, while EGR4 was shown adverse expression pattern. Survival analysis revealed up-regulated EGR1-4 were remarkably associated with favorable relapse-free survival (RFS). A close correlation with specific tumor-infiltrating immune cells (TIICs) and several CpG sites of EGRs were exhibited. Immunohistochemistry assays showed that the protein expression of EGR1 was remarkably downregulated in BRCA compared to that in paracancerous tissues. Cell migration of MCF10A cells was increased after the silence of EGR1 by siRNA transfection.Conclusions: This study provides a novel insight to the role of EGR1 in the prognostic value and cell migration of BRCA.

scholarly journals The transcription factor early growth response factor-1 (EGR-1) promotes apoptosis of neuroblastoma cells

2003 ◽  
Vol 373 (3) ◽  
pp. 739-746 ◽  
Author(s):  
Miguel PIGNATELLI ◽  
Rosario LUNA-MEDINA ◽  
Arturo PÉREZ-RENDÓN ◽  
Angel SANTOS ◽  
Ana PEREZ-CASTILLO
Keyword(s):  
Growth Response ◽  
P53 Protein ◽  
Early Growth ◽  
Early Gene ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Response Factor ◽  
P53 Family ◽  
Early Growth Response ◽  
In Cells

Early growth response factor-1 (EGR-1) is an immediate early gene, which is rapidly activated in quiescent cells by mitogens or in postmitotic neurons after depolarization. EGR-1 has been involved in diverse biological functions such as cell growth, differentiation and apoptosis. Here we report that enforced expression of the EGR-1 gene induces apoptosis, as determined by flow cytometry and terminal deoxynucleotidyl transferase-mediated dUTP-fluorescein nick-end labelling (TUNEL) analysis, in murine Neuro2A cells. In accordance with this role of EGR-1 in cell death, antisense oligonucleotides increase cell viability in cells cultured in the absence of serum. This apoptotic activity of the EGR-1 appears to be mediated by p73, a member of the p53 family of proteins, since an increase in the amount of p73 is observed in clones stably expressing the EGR-1 protein. We also observed an increase in the transcriptional activity of the mdm2 promoter in cells overexpressing EGR-1, which is paralleled by a marked decrease in the levels of p53 protein, therefore excluding a role of this protein in mediating EGR-1-induced apoptosis. Our results suggest that EGR-1 is an important factor involved in neuronal apoptosis.

scholarly journals Regulation and action of early growth response 1 in bovine granulosa cells

Reproduction ◽  
2017 ◽  
Vol 154 (4) ◽  
pp. 547-557 ◽  
Author(s):  
Peng Han ◽  
Hilda Guerrero-Netro ◽  
Anthony Estienne ◽  
Binyun Cao ◽  
Christopher A Price
Keyword(s):  
Granulosa Cells ◽  
Tyrosine Kinases ◽  
Growth Response ◽  
Early Growth ◽  
Mrna Abundance ◽  
Mrna Levels ◽  
Early Growth Response ◽  
Cell Proliferation And Differentiation ◽  
Early Growth Response 1

Fibroblast growth factors (FGF) modify cell proliferation and differentiation through receptor tyrosine kinases, which stimulate the expression of transcription factors including members of the early growth response (EGR) family. In ovarian granulosa cells, most FGFs activate typical response genes, although the role of EGR proteins has not been described. In the present study, we determined the regulation of EGR mRNA by FGFs and explored the role of EGR1 in the regulation of FGF-response genes. Addition of FGF1, FGF2, FGF4 or FGF8b increased EGR1 and EGR3 mRNA levels, whereas FGF18 increased only EGR1 mRNA abundance. No mRNA encoding EGR2 or EGR4 was detected. Overexpression of EGR1 increased EGR3 mRNA levels as well as the FGF-response genes SPRY2, NR4A1 and FOSL1 and also increased the phosphorylation of MAPK3/1. Knockdown of EGR3 did not alter the ability of FGF8b to stimulate SPRY2 mRNA levels. These data demonstrate the regulation of EGR1 and EGR3 mRNA abundance by FGFs in granulosa cells and suggest that EGR1 is likely an upstream component of FGF signaling in granulosa cells.

scholarly journals Role of Early Growth Response-1 (Egr-1) in Interleukin-13-induced Inflammation and Remodeling

2006 ◽  
Vol 281 (12) ◽  
pp. 8161-8168 ◽  
Author(s):  
Soo Jung Cho ◽  
Min Jong Kang ◽  
Robert J. Homer ◽  
Hye Ryun Kang ◽  
Xuchen Zhang ◽  
...  
Keyword(s):  
Growth Response ◽  
Early Growth ◽  
Early Growth Response ◽  
Interleukin 13 ◽  
Early Growth Response 1

scholarly journals p21Waf1/Cip1 Expression by Curcumin in U-87MG Human Glioma Cells: Role of Early Growth Response-1 Expression

2008 ◽  
Vol 68 (5) ◽  
pp. 1369-1377 ◽  
Author(s):  
Byeong Hyeok Choi ◽  
Chang Gun Kim ◽  
Young-Seuk Bae ◽  
Yoongho Lim ◽  
Young Han Lee ◽  
...  
Keyword(s):  
Growth Response ◽  
Glioma Cells ◽  
Early Growth ◽  
Human Glioma ◽  
Human Glioma Cells ◽  
Early Growth Response ◽  
Early Growth Response 1

scholarly journals O-032. The role of early growth response family of transcription factors in male fertility

1999 ◽  
Vol 14 (Suppl_3) ◽  
pp. 18-18
Author(s):  
C. Müller ◽  
A. Auyeung ◽  
C. Dorn ◽  
W. Tourtellotte ◽  
J. Milbrandt
Keyword(s):  
Transcription Factors ◽  
Male Fertility ◽  
Growth Response ◽  
Early Growth ◽  
Early Growth Response
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