scholarly journals Strawberry extract attenuates oxidative stress‐induced impaired insulin signaling in vitro in Human Skeletal Muscle Cells

2010 ◽  
Vol 24 (S1) ◽  
Author(s):  
Krishnankutty Sandhya ◽  
Ravi Tadapaneni ◽  
Katie Banaszewski ◽  
Jack Cappozzo ◽  
Indika Edirisinghe ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Skeletal Muscle ◽  
Insulin Signaling ◽  
Human Skeletal Muscle ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Human Skeletal Muscle Cells ◽  
Impaired Insulin

scholarly journals Silk fibroin scaffolds with muscle-like elasticity support in vitro differentiation of human skeletal muscle cells

2016 ◽  
Vol 11 (11) ◽  
pp. 3178-3192 ◽  
Author(s):  
Vishal Chaturvedi ◽  
Deboki Naskar ◽  
Beverley F. Kinnear ◽  
Elizabeth Grenik ◽  
Danielle E. Dye ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Silk Fibroin ◽  
Human Skeletal Muscle ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
In Vitro Differentiation ◽  
Human Skeletal Muscle Cells

scholarly journals Metabolic switching of human skeletal muscle cells in vitro

2011 ◽  
Vol 85 (5) ◽  
pp. 227-234 ◽  
Author(s):  
G.H. Thoresen ◽  
N.P. Hessvik ◽  
S.S. Bakke ◽  
V. Aas ◽  
A.C. Rustan
Keyword(s):  
Skeletal Muscle ◽  
Human Skeletal Muscle ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Metabolic Switching ◽  
Human Skeletal Muscle Cells

scholarly journals Impairment of Insulin Signaling in Human Skeletal Muscle Cells by Co-Culture With Human Adipocytes

Diabetes ◽  
2002 ◽  
Vol 51 (8) ◽  
pp. 2369-2376 ◽  
Author(s):  
D. Dietze ◽  
M. Koenen ◽  
K. Rohrig ◽  
H. Horikoshi ◽  
H. Hauner ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Insulin Signaling ◽  
Human Skeletal Muscle ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Human Adipocytes ◽  
Human Skeletal Muscle Cells

scholarly journals Strawberry extract attenuates glucose and free fatty acid‐mediated impaired insulin signaling in vitro in skeletal muscle cells

2012 ◽  
Vol 26 (S1) ◽  
Author(s):  
Archana Kangath ◽  
Claire Chang ◽  
Sandhya Krishnankutty ◽  
Ravi Kiran Tadapaneni ◽  
Indika Edirisinghe ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Fatty Acid ◽  
Free Fatty Acid ◽  
Insulin Signaling ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Impaired Insulin

scholarly journals Impact of Nucleoside Reverse Transcriptase Inhibitors on Mitochondrial DNA and RNA in Human Skeletal Muscle Cells

2008 ◽  
Vol 52 (8) ◽  
pp. 2825-2830 ◽  
Author(s):  
Akihiko Saitoh ◽  
Richard H. Haas ◽  
Robert K. Naviaux ◽  
Neurita G. Salva ◽  
Justine K. Wong ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Mitochondrial Dna ◽  
Reverse Transcriptase ◽  
Human Skeletal Muscle ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Reverse Transcriptase Inhibitors ◽  
Human Skeletal Muscle Cells ◽  
Hiv 1

ABSTRACT We previously reported that 2′,3′-dideoxyinosine (didanosine, or ddI) significantly altered mitochondrial DNA (mtDNA) in peripheral blood mononuclear cells in human immunodeficiency virus type 1 (HIV-1)-infected children who had undetectable plasma HIV-1 RNA for more than 2 years while receiving highly active antiretroviral therapy. This research examines the in vitro effects of nucleoside reverse transcriptase inhibitors (NRTIs) on mitochondria of human skeletal muscle cells (HSMCs), including myoblasts and differentiated myotubes. mtDNA, mitochondrial RNA (mtRNA), and mRNA levels for nuclear mitochondrial regulatory factors were quantified in vitro using HSMCs, including myoblasts and differentiated myotubes, treated with NRTIs singly and in combination. After 5 days of treatment, mtDNA was significantly decreased in myoblasts and myotubes treated with ddI (P < 0.001 and P = 0.01, respectively) and ddI-containing regimens (P < 0.001 and P < 0.001, respectively) compared to levels in untreated cells. mtRNA (MTCYB) was also significantly decreased in the myoblasts and myotubes treated with ddI (P = 0.004) and ddI-containing regimens (P < 0.001). Regardless of the NRTI regimens examined, NRTI combinations significantly decreased mtRNA (MTCO3) in myoblasts and myotubes (P = 0.02 and P = 0.01, respectively). No significant differences were observed for nuclear mitochondrial regulatory factor mRNA in myoblasts or myotubes when treated with NRTIs (P > 0.07). ddI and ddI-containing regimens significantly decrease mtDNA and mtRNA in HSMCs, most notably in myoblasts. These findings may be of particular importance in developing countries, where ddI is widely used for first-line treatment of HIV-infected children.

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