Antioxidant and antimicrobial activities of three different solvent extracts of guava leaf (Psidium guajava L.)

Abstract Psidium guajava (guava) is predominantly grown in India and known for its medicinal properties in treating various diseases and disorders. The present work focuses on aqueous extraction of bioactive compounds from the guava leaf and its utilization in the formulation of jelly to improve the public health. The guava leaf extract has been used in the preparation of jelly with pectin (1.5 g), sugar (28 g) and lemon juice (2 mL). The prepared guava leaf extract jelly (GJ) and the control jelly (CJ, without extract) were subjected to proximate, nutritional and textural analyses besides determination of antioxidant and antimicrobial activities. GJ was found to contain carbohydrate (45.78 g/100 g), protein (3.0 g/100 g), vitamin C (6.15 mg/100 g), vitamin B3 (2.90 mg/100 g) and energy (120.6 kcal). Further, the texture analysis of CJ and GJ indicated that both the jellies showed similar properties emphasizing that the addition of guava leaf extract does not bring any change in the texture properties of jelly. GJ exhibited antimicrobial activity against various bacteria ranging from 11.4–13.6 mm. Similarly, GJ showed antioxidant activity of 42.38% against DPPH radical and 33.45% against hydroxyl radical. Mass spectroscopic analysis of aqueous extract confirmed the presence of esculin, quercetin, gallocatechin, 3-Sinapoylquinic acid, gallic acid, citric acid and ellagic acid which are responsible for antioxidant and antimicrobial properties.

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Phytochemical Screening, Antioxidant and Antimicrobial Activities of the Essential Oils and Ethanol Extract of Psidium guajava Leaf

Asian Journal of Physical and Chemical Sciences ◽

10.9734/ajopacs/2019/v7i430102 ◽

2019 ◽

pp. 1-8

Author(s):

Andrew Emmanuel ◽

Dimas Kubmarawa ◽

Galo Yahaya Sara ◽

Akurenda Wahu

Keyword(s):

Essential Oil ◽

Ethanol Extract ◽

Psidium Guajava ◽

Antimicrobial Activities ◽

Herbal Remedies ◽

Diffusion Method ◽

Phytochemical Screening ◽

Standard Drug ◽

Dpph Method ◽

Antioxidant And Antimicrobial Activities

Objective: To investigate the Phytochemical screening, antioxidant and antimicrobial activities of the essential oil and ethanol extract of Psidium guajava. Methods: The leaf of Psidium guajavabelongs to the myrtle family (Myrtacease) which is used as herbal remedies for the cure of many ailments by natives in northern part of Nigeria, was collected in June, 2018 from the Professor’s Quarters of Modibbo Adama University of Technology (MAUTECH) Yola. The leaf was air dried, pulverized and extracted by simple overnight maceration technique and then analyzed. Fresh leaf of the aforementioned was extracted using modified steam distillation. The phytochemical screening of the ethanol extract was carried out using standard method. Results: The result revealed the present of Tannin, Flavanoid, Alkaloid, Volatile oil, Triterpene, Saponin, Glycoside while phenolic compound was absent in the ethanol extract of Psidium guajava. The result of the antioxidant activity of the essential oil was screened using DPPH method and the IC25 values of ascorbic acid (standard drug) was 57.92 µl/m and Psidium guajava of the essential oil was 46.55 µl/ml respectively. Antibacterial activity was carried out using dics diffusion method and the results showed reasonable zone of inhibition against tasted organisms, with Staphylococcus epidemidis being the most inhibited (23 mm) and Proteus vulgari being the least inhibited (2 mm) with the ethanol extract of Psidium guajava. In contrast, Staphylococcus aureus was the most inhibited (13 mm) and Salmonella typhi showed the least inhibition (9mm) in the essential oil of Psidium guajava. Conclusion: The result, thus support the use of the plants traditionally to treat chronic diarrhea, fever, diabetes, malaria and suggest its usage in the formulation of new antioxidant and antibacterial drugs.

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A BIOPROCESS DEVELOPMENT STUDY OF POLYPHENOL PROFILE, ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF KOMBUCHA ENRICHED WITH Psidium guajava L.

Journal of Microbiology Biotechnology and Food Sciences ◽

10.15414/jmbfs.2020.9.6.1204-1210 ◽

2020 ◽

Vol 9 (6) ◽

pp. 1204-1210

Author(s):

Mona M. Khaleil

Keyword(s):

Psidium Guajava ◽

Antimicrobial Activities ◽

Bioprocess Development ◽

Development Study ◽

Polyphenol Profile ◽

Antioxidant And Antimicrobial Activities

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Antioxidant and antimicrobial activities of Psidium guajava L. spray dried extracts

Industrial Crops and Products ◽

10.1016/j.indcrop.2014.05.049 ◽

2014 ◽

Vol 60 ◽

pp. 39-44 ◽

Cited By ~ 22

Author(s):

M.R.V. Fernandes ◽

A.L.T. Dias ◽

R.R. Carvalho ◽

C.R.F. Souza ◽

W.P. Oliveira

Keyword(s):

Psidium Guajava ◽

Antimicrobial Activities ◽

Antioxidant And Antimicrobial Activities ◽

Spray Dried

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Extraction of bioactive compounds from Psidium guajava leaves and its utilization in preparation of jellies

AMB Express ◽

10.1186/s13568-021-01194-9 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

N. S. Sampath Kumar ◽

Norizah Mhd Sarbon ◽

Sandeep Singh Rana ◽

Anjani Devi Chintagunta ◽

S. Prathibha ◽

...

Keyword(s):

Bioactive Compounds ◽

Leaf Extract ◽

Antimicrobial Properties ◽

Psidium Guajava ◽

Antimicrobial Activities ◽

Lemon Juice ◽

Vitamin B3 ◽

The Public ◽

Guava Leaf ◽

Textural Analyses

AbstractPsidium guajava L. (guava) is predominantly grown throughout the world and known for its medicinal properties in treating various diseases and disorders. The present work focuses on aqueous extraction of bioactive compounds from the guava leaf and its utilization in the formulation of jelly to improve the public health. The guava leaf extract has been used in the preparation of jelly with pectin (1.5 g), sugar (28 g) and lemon juice (2 mL). The prepared guava leaf extract jelly (GJ) and the control jelly (CJ, without extract) were subjected to proximate, nutritional and textural analyses besides determination of antioxidant and antimicrobial activities. GJ was found to contain carbohydrate (45.78 g/100 g), protein (3.0 g/100 g), vitamin C (6.15 mg/100 g), vitamin B3 (2.90 mg/100 g) and energy (120.6 kcal). Further, the texture analysis of CJ and GJ indicated that both the jellies showed similar properties emphasizing that the addition of guava leaf extract does not bring any change in the texture properties of jelly. GJ exhibited antimicrobial activity against various bacteria ranging from 11.4 to 13.6 mm. Similarly, GJ showed antioxidant activity of 42.38% against DPPH radical and 33.45% against hydroxyl radical. Mass spectroscopic analysis of aqueous extract confirmed the presence of esculin, quercetin, gallocatechin, 3-sinapoylquinic acid, gallic acid, citric acid and ellagic acid which are responsible for antioxidant and antimicrobial properties.

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