scholarly journals PLASMA SULFURED AMINO ACID RESPONSES TO METHIONINE‐LOADING TEST IN HIV+ PATIENTS

2015 ◽  
Vol 29 (S1) ◽  
Author(s):  
Roberto Burini ◽  
Maria D Borges‐Santos ◽  
Fernando Moreto ◽  
Paulo Pereira ◽  
Yong Ming‐Yu
Keyword(s):  
Amino Acid ◽  
Loading Test ◽  
Hiv Patients ◽  
Methionine Loading Test

Validation of abbreviated oral methionine-loading test

1995 ◽  
Vol 41 (6) ◽  
pp. 948-949 ◽  
Author(s):  
A G Bostom ◽  
R Roubenoff ◽  
P Dellaripa ◽  
M R Nadeau ◽  
P Sutherland ◽  
...  
Keyword(s):  
Loading Test ◽  
Methionine Loading Test

scholarly journals l-Folic Acid Supplementation in Healthy Postmenopausal Women: Effect on Homocysteine and Glycolipid Metabolism

2005 ◽  
Vol 90 (8) ◽  
pp. 4622-4629 ◽  
Author(s):  
Paola Villa ◽  
Concetta Perri ◽  
Rosanna Suriano ◽  
Francesco Cucinelli ◽  
Simona Panunzi ◽  
...  
Keyword(s):  
Folic Acid ◽  
Postmenopausal Women ◽  
Area Under The Curve ◽  
Density Lipoprotein ◽  
Folic Acid Supplementation ◽  
Oral Glucose ◽  
Loading Test ◽  
Acid Supplementation ◽  
Hepatic Insulin Extraction ◽  
Methionine Loading Test

Context: Hyperhomocysteinemia as well as alterations of glycemic and lipidic metabolism are recognized as risk factors for cardiovascular diseases. Objective: The aim of this study was to examine the effect of l-folic acid supplementation on homocysteine (Hcy) and related thiols, such as cysteine (Cys) and Cys-glycine (Cys-Glyc) pathways and their relationship to glucose, insulin, and lipidic metabolism in normoinsulinemic postmenopausal women. Design: This study was a randomized placebo, not double-blind, trial. Setting: The study was performed in an academic research center. Patients or Other Participants: Twenty healthy postmenopausal women were selected. No patient was taking drugs known to affect lipid or glucose metabolism. Intervention(s): Patients underwent two hospitalizations before and after 8 wk of l-acid folic (7.5 mg/d) or placebo administration. The glycemic metabolism was studied by an oral glucose tolerance test and a hyperinsulinemic euglycemic clamp. Hcy metabolism was studied by a standardized oral methionine-loading test. Main Outcome Measure(s): Hcy, Cys, and Cys-Glyc, basally and after a methionine loading test, were measured. Basal insulin, glucose, and peptide C levels as well as area under the curve for insulin, area under the curve for peptide, hepatic insulin extraction, and metabolic index were assayed. The total cholesterol, high-density lipoprotein (HDL) cholesterol, and low-density lipoprotein (LDL) cholesterol levels and the cholesterol/HDL and LDL/HDL ratios were also measured. Results: The total basal Hcy concentration and the plasma postmethionine loading Hcy values were significantly decreased (P < 0.01) in l-folic acid-treated patients, whereas postmethionine loading Cys-Glyc levels were markedly increased (P < 0.02). Furthermore, l-folic acid intake induced a significant improvement in carbohydrate metabolism through an increase in fractional hepatic insulin extraction (P < 0.05) and peripheral insulin sensitivity (P < 0.02) in normoinsulinemic women. HDL levels considerably increased, inducing an improvement in other atherosclerotic indexes, such as cholesterol/HDL and LDL/HDL ratios (P < 0.03). Conclusions: These results show that folic acid supplementation lowers plasma Hcy levels and improves insulin and lipid metabolism, reducing the risk of cardiovascular disease.

Ischemic strokes secondary to vitamin B12 deficiency-induced hyperhomocystinemia

Neurology ◽  
1998 ◽  
Vol 51 (2) ◽  
pp. 622-624 ◽  
Author(s):  
LaRoy P. Penix
Keyword(s):  
Mean Corpuscular Volume ◽  
Serum Vitamin ◽  
Vitamin B12 Deficiency ◽  
Vitamin B ◽  
Loading Test ◽  
Schilling Test ◽  
B12 Deficiency ◽  
Recurrent Strokes ◽  
Methionine Loading Test

A 45-year-old woman sustained two ischemic cerebral infarctions 16 years after ileal resection for Crohn's disease. Her evaluation showed an elevated random serum homocystine level, a low serum vitamin B12 level, and an increased mean corpuscular volume (MCV) without anemia. A methionine-loading test resulted in a marked increase in the homocystine levels 2, 4, and 6 hours after the load. A Schilling test demonstrated a malabsorption of vitamin B12. Vitamin B12 injections normalized her fasting homocystine level and her MCV. She has had no recurrent strokes during a year follow-up.

Genotype-independent in vivo oxidative stress following a methionine loading test: Maximal platelet activation in subjects with early-onset thrombosis

2011 ◽  
Vol 128 (4) ◽  
pp. e43-e48 ◽  
Author(s):  
Matteo Nicola Dario Di Minno ◽  
Salvatore Pezzullo ◽  
Vittorio Palmieri ◽  
Antonio Coppola ◽  
Armando D'Angelo ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Platelet Activation ◽  
Early Onset ◽  
Loading Test ◽  
Methionine Loading Test

Synthesis ofL-[3,3,4,4,S-methyl-2H7]methionine for use as a substrate for the methionine loading test

2001 ◽  
Vol 44 (1) ◽  
pp. 21-30 ◽  
Author(s):  
Hiroshi Hasegawa ◽  
Yoshihiko Shinohara ◽  
Kazunori Tagoku ◽  
Takao Hashimoto
Keyword(s):  
Loading Test ◽  
Methionine Loading Test

Fasting and Post-methionine Homocysteine Levels in Alzheimer’s Disease and Vascular Dementia

2009 ◽  
Vol 79 (3) ◽  
pp. 166-172 ◽  
Author(s):  
Paola Villa ◽  
Paolo Bosco ◽  
Raffaele Ferri ◽  
Concetta Perri ◽  
Rosanna Suriano ◽  
...  
Keyword(s):  
Vascular Dementia ◽  
Neuronal Degeneration ◽  
Cognitive Status ◽  
Control Group ◽  
Loading Test ◽  
Dementia Patients ◽  
Percent Increase ◽  
Normal Controls ◽  
Methionine Loading Test

The aim of the study is to compare the basal homocysteine levels in patients with impairment of cognitive status, and in controls, to evaluate if the methionine loading test is able to identify any differences between patients with Alzheimer’s disease and patients with vascular dementia. We enrolled 56 subjects, 20 with Alzheimer’s disease, 18 with vascular dementia, and 18 normal controls. The data shown that plasma homocysteine levels both basal and post-methionine load were significantly higher in the two groups of demented patients than in the control group. No significant differences were found between Alzheimer’s patients and vascular dementia patients. The homocysteine percent increase after a methionine loading test was significantly higher in the controls with respect to the two groups of demented patients. Only in Alzheimer’s patients were vitamin B12 basal levels negatively correlated with basal homocysteine levels (p<0.05), while positively correlated with the homocysteine percent increase after load (p<0.05). The study confirms the possible role of chronically elevated homocysteinemia in neuronal degeneration in demented patients. Even if the methionine loading test revealed an abnormal homocysteine metabolism in demented patients, it didn’t show any difference among patients with Alzheimer’s disease and vascular dementia.

scholarly journals MACROPHAGE INFLAMMATORY PROTEIN 2-Alpha (MIP-2) IN HIV PATIENTS LEADING TO LUNG INJURY AFTER Pneumocystis jiroveci INFECTION

2015 ◽  
Vol 2 (1) ◽  
pp. 58
Author(s):  
Rizqa Febriliany Putri ◽  
Egtheastraqita Chitivema ◽  
Marwoto Marwoto ◽  
Afiono Agung Prasetyo
Keyword(s):  
Amino Acid ◽  
Lung Injury ◽  
Untranslated Region ◽  
Consensus Sequence ◽  
Pulmonary Injury ◽  
Hiv Patients ◽  
Pneumocystis Jiroveci ◽  
Coding Sequences ◽  
Inflammatory Protein ◽  
Macrophage Inflammatory Protein

Introduction: Exuberant inflammation during Pneumonia caused by Pneumocystis jiroveci strongly promotes pulmonary injury in HIV patients and suggested caused by Macrophage Inflammatory Protein 2- Alpha (MIP-2) as a strong chemoattractant.Aims: To understanding the potential roles of MIP-2 in the pulmonary injury in HIV patients.Methods: All complete coding sequences of human MIP-2 deposited inGenBank were downloadedand subjected for bioinformatics analysis.Results: Human MIP-2 lacks the highly conserved purine at position -3 but possess many features of the consensus sequence. The consensus polyadenylation signal was present in the hu-MIP-2a cDNA at position 1182-1187of the 3' untranslated region followed by a poly(A) beginning at nucleotide 1202. The cysteine alignment and the presence of non conserved amino acid were defining the properties of chemokines in case of what substances that could be attracted. The mechanism of lung injury perhaps does not caused by alteration of MIP-2 directly. Keywords: MIP-2, HIV, lung injury, Pneumocystis jiroveci 

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