Bovine Pericardium Versus Homologous Sciera as Wrapping Materials for Hydroxyapatite Ocular Implants

The present study aimed to estimate the efficiency of both a cellular bovine pericardium and bovine urinary bladder matrix sheets in the reconstruction of large ventro-lateral hernias in Iraqi bucks by using of molecular evaluation depending on real time-polymerase chain reaction technique to investigate the level of basic-fibroblast growth factor and vascular endothelial growth factor genes during the healing process and reconstruction of the abdominal defects. Under sedation and local anesthesia, (6cm X 8cm size) of ventro-lateral hernias were induced in 24 of Iraqi bucks. The animals were divided randomly into two main equal groups. In bovine pericardium-treatment group, the hernias were treated with onlay implantation of bovine pericardium. While, the hernias in UBM-treatment group were treated with onlay implantation of urinary bladder matrix, 30 days post-inducing of hernias. The molecular evaluation along the period of following-up recorded a significant up-regulation of the level of basic-fibroblast growth factor gene specific for presence of fibroblasts, myofibroblasts and collagen deposition in urinary bladder matrix -treatment group in comparison to bovine pericardium -treatment group with significant difference even at the end of the study. While, a significant up regulation of the levels of angiogenesis classic gene vascular endothelial growth factor were recorded in the bucks of bovine pericardium -treatment group compared to urinary bladder matrix -treatment group. In conclusion; molecular detection of the level of growth factors in target tissue can be used as an important criterion.

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Biocompatiblibility analysis of the decellularized bovine pericardium

Cell and Organ Transplantology ◽

10.22494/cot.v8i2.110 ◽

2020 ◽

Vol 8 (2) ◽

Author(s):

A. Sokol ◽

◽

D. Grekov ◽

G. Yemets ◽

O. Galkin ◽

...

Keyword(s):

Tissue Engineering ◽

Sodium Dodecyl ◽

Bovine Pericardium ◽

Surrounding Tissue ◽

Tissue Samples ◽

Group I ◽

Alternative Material ◽

Decellularized Scaffolds ◽

Group 2 ◽

Potential Use

The decellularized bovine pericardium and its potential use as a natural scaffold is a promising approach in the field of tissue engineering and regenerative medicine. The reaction of the host toward decellularized scaffolds depends on their biocompatibility, which should be satisfied being before applied in clinical use. Purpose: to evaluate the biocompatibility of the extracellular matrices, which were decellularized by trypsin enzyme and anionic sodium dodecyl sulfate (SDS) detergent. Material and methods. Pericardial sacs were acquired from 12-18 months’ age bulls. Tissue decellularization was performed by using 0.25 % Trypsin solution and 1 % ionic SDS for group I and 0.1 % SDS for group II samples. The implantation was performed on Wistar rats. The tissue samples were stained with hematoxylin & eosin, Congo red and Masson's Trichrome for histological analysis. Results. In group 1 in 3 months after subcutaneous implantation in rats we noticed the inflammation in surrounding tissue and degradation of the implant. Under the same conditions in animals of group 2 implant replacement with growing immature connective tissue was noted. Bio-implant of this group did not degrade, moreover it's integrated to the tissues of experimental rats. Conclusion. Our results showed that decellularized bovine pericardium by 0.1 % SDS can become an alternative material for tissue engineering and has the potential for further use in human surgery.

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In vitro dissolution testing models of ocular implants for posterior segment drug delivery

Drug Delivery and Translational Research ◽

10.1007/s13346-021-01043-z ◽

2021 ◽

Author(s):

Muhammad Faris Adrianto ◽

Febri Annuryanti ◽

Clive G. Wilson ◽

Ravi Sheshala ◽

Raghu Raj Singh Thakur

Keyword(s):

Drug Release ◽

Posterior Segment ◽

In Vitro Dissolution ◽

Prolonged Treatment ◽

Term Therapy ◽

In Vitro Test ◽

Dissolution Testing ◽

Vitro Test ◽

Ocular Implants

AbstractThe delivery of drugs to the posterior segment of the eye remains a tremendously difficult task. Prolonged treatment in conventional intravitreal therapy requires injections that are administered frequently due to the rapid clearance of the drug molecules. As an alternative, intraocular implants can offer drug release for long-term therapy. However, one of the several challenges in developing intraocular implants is selecting an appropriate in vitro dissolution testing model. In order to determine the efficacy of ocular implants in drug release, multiple in vitro test models were emerging. While these in vitro models may be used to analyse drug release profiles, the findings may not predict in vivo retinal drug exposure as this is influenced by metabolic and physiological factors. This review considers various types of in vitro test methods used to test drug release of ocular implants. Importantly, it discusses the challenges and factors that must be considered in the development and testing of the implants in an in vitro setup. Graphical abstract

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Reply To Invited Discussion On: The Bovine Pericardium Matrix In Immediate Implant-Based Breast Reconstruction

Aesthetic Plastic Surgery ◽

10.1007/s00266-020-02114-1 ◽

2021 ◽

Author(s):

Fabio Castagnetti ◽

Chiara Bertani ◽

Monica Foroni ◽

Giuseppe Falco ◽

Eugenio Cenini ◽

...

Keyword(s):

Breast Reconstruction ◽

Bovine Pericardium ◽

Immediate Implant

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Invited Response on: Reply to Invited Discussion on—The Bovine Pericardium Matrix in Immediate Implant-Based Breast Reconstruction

Aesthetic Plastic Surgery ◽

10.1007/s00266-021-02191-w ◽

2021 ◽

Author(s):

Kamran Khoobehi

Keyword(s):

Breast Reconstruction ◽

Bovine Pericardium ◽

Immediate Implant

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Mapping of bovine pericardium to enable a standardized acquirement of material for medical implants

Journal of the Mechanical Behavior of Biomedical Materials ◽

10.1016/j.jmbbm.2021.104432 ◽

2021 ◽

pp. 104432

Author(s):

Felix Stieglmeier ◽

Maximilian Grab ◽

Fabian König ◽

Joscha Büch ◽

Christian Hagl ◽

...

Keyword(s):

Bovine Pericardium ◽

Medical Implants

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FLIm and Raman Spectroscopy for Investigating Biochemical Changes of Bovine Pericardium upon Genipin Cross-Linking

Molecules ◽

10.3390/molecules25173857 ◽

2020 ◽

Vol 25 (17) ◽

pp. 3857

Author(s):

Tanveer Ahmed Shaik ◽

Alba Alfonso-Garcia ◽

Martin Richter ◽

Florian Korinth ◽

Christoph Krafft ◽

...

Keyword(s):

Raman Spectroscopy ◽

Fluorescence Lifetime ◽

Density Functional ◽

Bovine Pericardium ◽

Biochemical Changes ◽

Blue Pigment ◽

Cross Linking ◽

Fluorescence Lifetime Imaging ◽

Fluorescence Intensity Ratio ◽

Cross Linker

Biomaterials used in tissue engineering and regenerative medicine applications benefit from longitudinal monitoring in a non-destructive manner. Label-free imaging based on fluorescence lifetime imaging (FLIm) and Raman spectroscopy were used to monitor the degree of genipin (GE) cross-linking of antigen-removed bovine pericardium (ARBP) at three incubation time points (0.5, 1.0, and 2.5 h). Fluorescence lifetime decreased and the emission spectrum redshifted compared to that of uncross-linked ARBP. The Raman signature of GE-ARBP was resonance-enhanced due to the GE cross-linker that generated new Raman bands at 1165, 1326, 1350, 1380, 1402, 1470, 1506, 1535, 1574, 1630, 1728, and 1741 cm−1. These were validated through density functional theory calculations as cross-linker-specific bands. A multivariate multiple regression model was developed to enhance the biochemical specificity of FLIm parameters fluorescence intensity ratio (R2 = 0.92) and lifetime (R2 = 0.94)) with Raman spectral results. FLIm and Raman spectroscopy detected biochemical changes occurring in the collagenous tissue during the cross-linking process that were characterized by the formation of a blue pigment which affected the tissue fluorescence and scattering properties. In conclusion, FLIm parameters and Raman spectroscopy were used to monitor the degree of cross-linking non-destructively.

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