Aloe vera Gel: Effective Therapeutic Agent against Multidrug-Resistant Pseudomonas aeruginosa Isolates Recovered from Burn Wound Infections

Objective. Aloe vera is an herbal medicinal plant with biological activities, such as antimicrobial, anticancer, anti-inflammatory, and antidiabetic ones, and immunomodulatory properties. The purpose of this study was investigation of in vitro antimicrobial activity of A. vera gel against multidrug-resistant (MDR) Pseudomonas aeruginosa isolated from patients with burn wound infections. Methods. During a 6-month study, 140 clinical isolates of P. aeruginosa were collected from patients admitted to the burn wards of a hospital in Tehran, Iran. Antimicrobial susceptibility test was carried out against the pathogens using the A. vera gel and antibiotics (imipenem, gentamicin, and ciprofloxacin). Results. The antibiogram revealed that 47 (33.6%) of all isolates were MDR P. aeruginosa. The extract isolated from A. vera has antibacterial activity against all of isolates. Also, 42 (89.4%) isolates were inhibited by A. vera gel extract at minimum inhibitory concentration (MIC) ≤ 200 µg/mL. MIC value of A. vera gel for other isolates (10.6%) was 800 µg/mL. All of MDR P. aeruginosa strains were inhibited by A. vera at similar MIC50 and MIC90 200 µg/mL. Conclusion. Based on our results, A. vera gel at various concentrations can be used as an effective antibacterial agent in order to prevent wound infection caused by P. aeruginosa.

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Enhanced Efficacy of Some Antibiotics in Presence of Silver Nanoparticles Against Multidrug Resistant Pseudomonas aeruginosa Recovered From Burn Wound Infections

Frontiers in Microbiology ◽

10.3389/fmicb.2021.648560 ◽

2021 ◽

Vol 12 ◽

Author(s):

Maha A. Khalil ◽

Gamal M. El Maghraby ◽

Fatma I. Sonbol ◽

Nanis G. Allam ◽

Perihan S. Ateya ◽

...

Keyword(s):

Pseudomonas Aeruginosa ◽

Silver Nanoparticles ◽

Pharmaceutical Formulations ◽

Inhibition Zone ◽

Multidrug Resistant ◽

Wound Infections ◽

Burn Wound ◽

Ag Nps

Burn wound infections with multidrug-resistant (MDR) bacteria are shown in many countries as severe widespread health threats. Consequently, attention has been devoted to new nanoparticle-based materials in the field of antimicrobial chemotherapy for burn wound infections. This study aimed to evaluate both in vitro and in vivo efficacies of nanoparticle–antibiotic combinations as new classes of materials subjected against MDR Pseudomonas aeruginosa. Out of 40 Gram-negative isolates, 23 P. aeruginosa were recovered from patients with burn wound infections attending different hospitals in Tanta, Egypt. The susceptibility test revealed that 95.7% of P. aeruginosa isolates were MDR with a high incidence of resistance against carbenicillin. Antibacterial activities of silver nanoparticles (Ag-NPs) against the isolates examined showed various inhibition zone diameters ranging from 11 to 17 mm. Strong synergistic efficacy of neomycin was reported in combination with Ag-NPs against MDR P. aeruginosa P8 and P14 isolates. The in vivo effectiveness of various pharmaceutical formulations prepared from a combination of neomycin antibiotic with Ag-NPs in the treatment of induced bacterially infected mice burns showed that maximum healing activity along with faster wound contraction reported with the combination of neomycin-Ag-NPs in the spray formulation. Generally, data indicated that incorporating Ag-NPs in combination with certain antibiotics may be a new, promising application for wound treatments, especially burns infected with MDR P. aeruginosa.

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Synergistic Action of Silver Sulfadiazine and Sodium Piperacillin on Resistant Pseudomonas aeruginosa in vitro and in Experimental Burn Wound Infections

Journal of Trauma and Acute Care Surgery ◽

10.1097/00005373-198501000-00005 ◽

1985 ◽

Vol 25 (1) ◽

pp. 27-31 ◽

Cited By ~ 13

Author(s):

SHANTA MODAK ◽

CHARLES L. FOX

Keyword(s):

Pseudomonas Aeruginosa ◽

Silver Sulfadiazine ◽

Synergistic Action ◽

Wound Infections ◽

Burn Wound ◽

Experimental Burn

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Antibacterial Activity of Aloe vera Gel against Multidrug Resistant Staphylococcus aureus and Pseudomonas aeruginosa

UMYU Journal of Microbiology Research (UJMR) ◽

10.47430/ujmr.2052.009 ◽

2020 ◽

pp. 74-80

Author(s):

Usman A. ◽

Keyword(s):

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Antibacterial Activity ◽

Wound Infection ◽

Aloe Vera ◽

Multidrug Resistant ◽

Diffusion Method ◽

Disk Diffusion Method ◽

Diffusion Technique ◽

Aloe Vera Gel

Staphylococcus aureus and Pseudomonas aeruginosa have been implicated as important nosocomial pathogens causing severe infections especially in hospitalized patients. The aim of the study was to assess the antibacterial activity of Aloe vera gel against multidrug resistant S. aureus and P. aeruginosa isolated from wound. Clinical isolates of S. aureus and P. aeruginosa from wound infection were collected from Microbiology laboratory of Barau Dikko Teaching Hospital (BDTH), Kaduna and re-confirmed using standard microbiological procedure. Antibiotic susceptibility pattern of the isolates was determined using Kirby Bauer disk diffusion method. Aloe vera gel was obtained fresh matured leaves of Aloe vera plant and was screened for the presence of phytochemical constituents. Antibacterial activity of the Aloe vera gel against Multidrug Resistant (MDR) Staphylococcus aureus and Pseudomonas aeruginosa isolates was determined by agar well diffusion technique. The result revealed that all the two isolates were resistant to more than three classes of antibiotics. The Staphylococcus aureus isolate was resistant to fluoroquinolone (ciprofloxacin), aminoglycoside (gentamicin), cephalosporin (cefaroline), folate pathway antagonist (trimethoprim-sulfamethoxazole), penicillin(cefoxitin) and macrolide (erythromycin) while the Pseudomonas aeruginosa isolate was resistant to βlactam (ticarcillin-clavulanate), fluoroquinolone (ciprofloxacin and norfloxacin), aminoglycoside (gentamicin) and cephalosporin (ceftazidime)hence regarded as MDR isolates. Phytochemical screening of the gel revealed the presence of saponins, flavonoids, terpenoids and alkaloids. The Aloe vera gel was found to have antibacterial activity against the test isolates with MIC and MBC values of 25µg/mL and 50µg/mL against MDR S. aureus 50µg/mL and 100µg/mL against MDR P. aeruginosa respectively. The study identified that Aloe vera gel possesses antibacterial activity against MDR S. aureus and P. aeruginosa isolated from wound infection. Keywords: Aloe vera gel, Staphylococcus aureus, Pseudomonas aeruginosa, antibacterial, MDR

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Effect of Human Burn Wound Exudate on Pseudomonas aeruginosa Virulence

mSphere ◽

10.1128/msphere.00111-15 ◽

2016 ◽

Vol 1 (2) ◽

Cited By ~ 17

Author(s):

Manuel R. Gonzalez ◽

Betty Fleuchot ◽

Leonardo Lauciello ◽

Paris Jafari ◽

Lee Ann Applegate ◽

...

Keyword(s):

Pseudomonas Aeruginosa ◽

Chemical Composition ◽

Composition Analysis ◽

Wound Infections ◽

Artificial Medium ◽

Burn Wound ◽

Content Type ◽

Burn Wounds ◽

Chemical Composition Analysis

ABSTRACT Microbial infection of severe burn wounds is currently a major medical challenge. Of the infections by bacteria able to colonize such injuries, those by Pseudomonas aeruginosa are among the most severe, causing major delays in burn patient recovery or leading to fatal issues. In this study, we investigated the growth properties of several burn wound pathogens in biological fluids secreted from human burn wounds. We found that P. aeruginosa strains were able to proliferate but not those of the other pathogens tested. In addition, burn wound exudates (BWEs) stimulate the expression of virulence factors in P. aeruginosa. The chemical composition analysis of BWEs enabled us to determine the major components of these fluids. These data are essential for the development of an artificial medium mimicking the burn wound environment and for in vitro analysis of the initial step in the development of burn wound infections. Burn wound sepsis is currently the main cause of morbidity and mortality after burn trauma. Infections by notorious pathogens such as Pseudomonas aeruginosa, Staphylococcus aureus, and Acinetobacter baumannii impair patient recovery and can even lead to fatality. In this study, we investigated the effect of burn wound exudates (BWEs) on the virulence of those pathogens. BWEs were collected within 7 days after burn trauma from 5 burn patients. We first monitored their effect on pathogen growth. In contrast to A. baumannii and S. aureus, P. aeruginosa was the only pathogen able to grow within these human fluids. Expression of typical virulence factors such as pyocyanin and pyoverdine was even enhanced compared the levels seen with standard laboratory medium. A detailed chemical composition analysis of BWE was performed, which enabled us to determine the major components of BWE and underline the metabolic modifications induced by burn trauma. These data are essential for the development of an artificial medium mimicking the burn wound environment and the establishment of an in vitro system to analyze the initial steps of burn wound infections. IMPORTANCE Microbial infection of severe burn wounds is currently a major medical challenge. Of the infections by bacteria able to colonize such injuries, those by Pseudomonas aeruginosa are among the most severe, causing major delays in burn patient recovery or leading to fatal issues. In this study, we investigated the growth properties of several burn wound pathogens in biological fluids secreted from human burn wounds. We found that P. aeruginosa strains were able to proliferate but not those of the other pathogens tested. In addition, burn wound exudates (BWEs) stimulate the expression of virulence factors in P. aeruginosa. The chemical composition analysis of BWEs enabled us to determine the major components of these fluids. These data are essential for the development of an artificial medium mimicking the burn wound environment and for in vitro analysis of the initial step in the development of burn wound infections.

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Study of Biofilm Formation and Antibiotic Resistant Profile in Multidrug Resistant and Extensive Drug Resistance Pseudomonas Aeruginosa Isolated from Burn Wound Infections in Southwest Iran

10.21203/rs.3.rs-795546/v2 ◽

2021 ◽

Keyword(s):

Pseudomonas Aeruginosa ◽

Drug Resistance ◽

Full Text ◽

Biofilm Formation ◽

Multidrug Resistant ◽

Wound Infections ◽

Burn Wound ◽

Antibiotic Resistant ◽

Extensive Drug Resistance ◽

Southwest Iran

Abstract The full text of this preprint has been withdrawn by the authors due to author disagreement with the posting of the preprint. Therefore, the authors do not wish this work to be cited as a reference. Questions should be directed to the corresponding author.

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Inhibitory effects of Cinnamaldehyde, Carvacrol, and honey on the expression of exoS and ampC genes in multidrug-resistant Pseudomonas aeruginosa isolated from burn wound infections

Microbial Pathogenesis ◽

10.1016/j.micpath.2019.103946 ◽

2020 ◽

Vol 140 ◽

pp. 103946 ◽

Cited By ~ 1

Author(s):

Zahra Mohammadzamani ◽

Ahmad Khorshidi ◽

Azad Khaledi ◽

Ali Shakerimoghaddam ◽

Gholam Abbas Moosavi ◽

...

Keyword(s):

Pseudomonas Aeruginosa ◽

Multidrug Resistant ◽

Wound Infections ◽

Inhibitory Effects ◽

Burn Wound

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Study of Biofilm Formation and Antibiotic Resistant Profile in Multidrug Resistant and Extensive Drug Resistance Pseudomonas Aeruginosa Isolated from Burn Wound Infections in Southwest Iran

10.21203/rs.3.rs-795546/v1 ◽

2021 ◽

Author(s):

Aram asareh zadegan dezfuli ◽

Arshid Yousefi Avarvand

Keyword(s):

Pseudomonas Aeruginosa ◽

Drug Resistance ◽

Biofilm Formation ◽

Multidrug Resistant ◽

Resistance Rate ◽

Wound Infections ◽

Burn Wound ◽

Antibiotic Resistant ◽

Extensive Drug Resistance ◽

Southwest Iran

Abstract Background: Pseudomonas aeruginosa is an opportunistic pathogen that has remained on the ‘top 10’ common hospital ‘superbugs’ worldwide for more than a decade. Study of biofilm formation and antibiotic resistant profile in multidrug resistant and extensive drug resistance P. aeruginosa isolated from burn wound infections in southwest IranMethods and Results: This study, which was performed in 110 P. aeruginosa isolates culture-positive reports. Assessment of biofilm formation via microtiteplate and congo red agar. Overall, 110 clinical P. aeruginosa isolates were confirmed from wound burn infections. The maximum resistance rate among P. aeruginosa isolates to antibiotics tested was as follow Piperacillin, ceftazidime, and minimum resistance rate among P. aeruginosa isolates to antibiotics tested such as ticarcillin-clavulanic acid. The isolates were then evaluating the MICs by using the E-test. only 7 isolates were confirmed as colistin-resistant. Colistin reference MICs for the The prevalence of MDR P. aeruginosa was 38% and XDR- P. aeruginosa was 22% respectively. One of P. aeruginosa isolates were PDR. In microtiteplate assay,76% of the isolates have ability for biofilm, formation, 40% were categorized as strong biofilm-formers; 32% were moderate; 21% were weak biofilm formers and 43% could not form any detectable biofilm. Conclusion: in our study development of resistance by P. aeruginosa to many antimicrobial agents is a great challenge in controlling its infections. Therefore, the transmission of these isolates to patients leads to higher resistance. Therefore, the necessary hygiene measurements should be taken for the prevention of transferring the P. aeruginosa isolates to hospitalized patients.

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Photoelimination Potential of Chitosan NanoparticlesIndocyanine Green Complex Against the Biological Activities of Acinetobacter baumannii Strains: A Preliminary In Vitro Study in Burn Wound Infections

Journal of lasers in medical sciences ◽

10.34172/jlms.2020.31 ◽

2020 ◽

Vol 11 (2) ◽

pp. 187-192 ◽

Cited By ~ 1

Author(s):

Maryam Pourhajibagher ◽

Nava Hosseini ◽

Ebrahim Boluki ◽

Nasim Chiniforush ◽

Abbas Bahador

Keyword(s):

Acinetobacter Baumannii ◽

Diode Laser ◽

Biological Activities ◽

Chitosan Nanoparticles ◽

Control Group ◽

Wound Infections ◽

Burn Wound ◽

Sem Images ◽

Burn Wounds

Introduction: Acinetobacter baumannii strains are important agents causing serious nosocomial infections including soft-tissue and skin infections in patients with burn wounds which have become resistant to several classes of antibiotics. Antimicrobial photodynamic therapy (aPDT) as an alternative antimicrobial procedure is suggested for the treatment of these kinds of infections. The aim of the current study is to evaluate the antibacterial and anti-biofilm efficiency of aPDT by the utilization of an improved form of indocyanine green (ICG) which is encapsulated in chitosan nanoparticles (NCs@ICG). Methods: NCs@ICG were synthesized and confirmed by the scanning electron microscope (SEM). aPDT was performed using NCs@ICG with an 810 nm wavelength of the diode laser at the fluency of 31.2 J/cm2 on 50 A. baumannii strains isolated from burn wounds. The antibacterial and antibiofilm potential of NCs@ICG-aPDT was determined via the colony forming unit (CFU)/mL and crystal violet assays, respectively. In addition, microbial biofilm degradation was evaluated by the SEM. Results: According to the results, NCs@ICG-aPDT showed a significant reduction of 93.2% on the CFU/ mL of planktonic A. baumannii strains compared to the control group (untreated group; P < 0.05). In addition, the biofilm formation of A. baumannii strains was significantly reduced by 55.3% when the bacteria were exposed to NCs@ICG-aPDT (P < 0.05). In contrast, NCs@ICG, ICG, and the diode laser alone were not able to inhibit the CFU/mL and biofilm of A. baumannii strains (P > 0.05). Based on the results of SEM images, NCs@ICG-aPDT disrupted the biofilm structure of A. baumannii strains more than other groups. Conclusion: NCs@ICG-aPDT demonstrates a promising treatment candidate for exploitation in wound infections against both planktonic and biofilm forms of A. baumannii strains

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Bacterial Colonization of Burn Wounds

Baghdad Science Journal ◽

10.21123/bsj.9.4.623-631 ◽

2012 ◽

Vol 9 (4) ◽

pp. 623-631 ◽

Cited By ~ 1

Author(s):

Baghdad Science Journal

Keyword(s):

Pseudomonas Aeruginosa ◽

Therapeutic Agent ◽

Bacterial Colonization ◽

Burn Patients ◽

Wound Infections ◽

Antimicrobial Susceptibility Test ◽

Burn Wound ◽

Bacteriological Profile ◽

Microbiological Methods ◽

Burns Unit

The objective behind this work was to find out the bacteriological profile of post burn infections in wound. The study was carried out from December 2010 to February 2011 at the Burns Unit of Al –Kindy Hospital and Al-Yarmook Hospital in Baghdad. Sixty burn patients have been investigated for bacterial profile of burn wound infections. Specimens were collected in the form of wound swabs. The organisms were isolated and identified by standard microbiological methods. Antimicrobial susceptibility test has been done by ATB-PSE5 kit(BioMereiux). Pseudomonas aeruginosa 35(58.3 %) was found to be the most common isolate followed by Klebsiella pneumonae 10(16.6%), Staphylococcus aureus 7(10%). ,E.coli 3(5%), Proteus merabilis 1(1.6 %), others 2(3.3 %).and sterile cultures 2(3.3 %). Antibiotic susceptibility pattren for Pseudomonas aeruginosa isolates have shown high susptibility for Amikacin 30(85.0%), Pipracillin-Tazobactam 27(77.2%) Piperacillin 25(71.4%), Ciprofloxaqcin 25(71.4%) and Colistin 24(68.5%) and low susceptibility for Tobramycin 20(57.2%) Gentamycin 18(51.0%). Psudomonas was found to be resistant to most of the therapeutic agent.

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