LONG-TERM EFFECTS ON HEK-293 CELL LINE AFTER CO-CULTURE WITH PORCINE ENDOGENOUS RETROVIRUS

A major concern in using porcine organs for transplantation is the potential of transmission of porcine endogenous retrovirus (PERV). To investigate the long-term effects of PERV infection on human cells, human embryonic kidney cell line HEK-293 infected with PERV PK-15 was maintained for up to 72 passages and samples were harvested at intervals for use in morphological, growth, and genomic analyses. Morphology, DNA content/cell, and doubling time of uninfected and infected cells were similar. Restriction fragment length polymorphism (RFLP) analysis of PCR-amplified nearly full-length PERV genome showed no alterations in band pattern. RFLP analysis of the long terminal repeats (LTR) showed some changes in band pattern, but not in length. Southern blot analysis of genomic DNA of infected cells indicated random integration of PERV without structural alterations in proviral genome. Semi-quantitative PCR demonstrated a gradual increase of proviral load in the infected cells. Sequence analysis of the LTR region of PERV from infected cells indicated a relatively low rate (6.0 × 104/bp or about 2 × 106/bp/generation) of mutation. There were also indications of recombination of PERV strains A and B. Finally, PERV infection had no effect on transcription of human endogenous retrovirus-K (HERV-K) genes. Together, no significant effect attributable to PERV infection was evident on chronically PERV-infected HEK-293 cells.Key words: porcine endogenous retrovirus (PERV), human endogenous retrovirus-K (HERV-K), xenotransplantation, zoonosis.

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The time course of Akt and ERK activation on XIAP expression in HEK 293 cell line

Molecular Biology Reports ◽

10.1007/s11033-009-9658-4 ◽

2009 ◽

Vol 37 (4) ◽

pp. 2037-2042 ◽

Cited By ~ 16

Author(s):

Mousa Abkhezr ◽

Ali Reza Keramati ◽

Seyed Nasser Ostad ◽

Jamshid Davoodi ◽

Mohammad H. Ghahremani

Keyword(s):

Cell Line ◽

Time Course ◽

Hek 293 ◽

Erk Activation ◽

Xiap Expression ◽

Hek 293 Cell Line

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Enhancing the Butyrylcholinesterase Activity in HEK-293 Cell Line by Dual-Promoter Vector Decorated on Lipofectamine

Drug Design Development and Therapy ◽

10.2147/dddt.s260419 ◽

2020 ◽

Vol Volume 14 ◽

pp. 3589-3599

Author(s):

Vida Mirzaie ◽

Touba Eslaminejad ◽

Homayoon Babaei ◽

Seyed Noureddin Nematollahi-Mahani

Keyword(s):

Cell Line ◽

Hek 293 ◽

Dual Promoter ◽

Butyrylcholinesterase Activity ◽

Hek 293 Cell Line

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Design, synthesis and biological evaluation of 1,2,3-triazole based 2-aminobenzimidazoles as novel inhibitors of LasR dependent quorum sensing in Pseudomonas aeruginosa

RSC Advances ◽

10.1039/c9ra05059k ◽

2019 ◽

Vol 9 (50) ◽

pp. 29273-29292 ◽

Cited By ~ 3

Author(s):

Singireddi Srinivasarao ◽

Adinarayana Nandikolla ◽

Shashidhar Nizalapur ◽

Tsz Tin Yu ◽

Sravani Pulya ◽

...

Keyword(s):

Pseudomonas Aeruginosa ◽

Quorum Sensing ◽

Cell Line ◽

Biological Evaluation ◽

Design Synthesis ◽

Hek 293 ◽

Line P ◽

Synthesis And Biological Evaluation ◽

Hek 293 Cell Line

Out of 40 benzimdazoles, 12 exhibited potent QSI activity against P. aeruginosa6p, most active QSI is docked to LasR and is less toxic against HEK 293 cell line.

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Genome-wide identification of target genes repressed by the zinc finger transcription factor REST/NRSF in the HEK 293 cell line

Acta Biochimica et Biophysica Sinica ◽

10.1093/abbs/gmp095 ◽

2009 ◽

Vol 41 (12) ◽

pp. 1008-1017 ◽

Cited By ~ 17

Author(s):

Z. Liu ◽

M. Liu ◽

G. Niu ◽

Y. Cheng ◽

J. Fei

Keyword(s):

Transcription Factor ◽

Cell Line ◽

Zinc Finger ◽

Target Genes ◽

Hek 293 ◽

Zinc Finger Transcription Factor ◽

Genome Wide ◽

Hek 293 Cell Line

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Extended Analysis of the In Vitro Tropism of Porcine Endogenous Retrovirus

Journal of Virology ◽

10.1128/jvi.74.1.49-56.2000 ◽

2000 ◽

Vol 74 (1) ◽

pp. 49-56 ◽

Cited By ~ 152

Author(s):

Carolyn A. Wilson ◽

Susan Wong ◽

Matthew VanBrocklin ◽

Mark J. Federspiel

Keyword(s):

Cell Line ◽

Cell Lines ◽

Human Cell ◽

Human Cell Line ◽

Endogenous Retrovirus ◽

Human Cells ◽

Productive Infection ◽

Porcine Endogenous Retrovirus ◽

Productive Replication

ABSTRACT We previously reported that mitogenic activation of porcine peripheral blood mononuclear cells resulted in production of porcine endogenous retrovirus(es) (PERV[s]) capable of productively infecting human cells (C. Wilson et al., J. Virol. 72:3082–3087, 1998). We now extend that analysis to show that additional passage of isolated virus, named here PERV-NIH, through a human cell line yielded a viral population with a higher titer of infectious virus on human cells than the initial isolate. We show that in a single additional passage on a human cell line, the increase in infectivity for human cells is accounted for by selection against variants carrying pig-tropic envelope sequences (PERV-C) as well as by enrichment for replication-competent genomes. Sequence analysis of the envelope cDNA present in virions demonstrated that the envelope sequence of PERV-NIH is related to but distinct from previously reported PERV envelopes. The in vitro host range of PERV was studied in human primary cells and cell lines, as well as in cell lines from nonhuman primate and other species. This analysis reveals three patterns of susceptibility to infection among these host cells: (i) cells are resistant to infection in our assay; (ii) cells are infected by virus, as viral RNA is detected in the supernatant by reverse transcription-PCR, but the cells are not permissive to productive replication and spread; and (iii) cells are permissive to low-level productive replication. Certain cell lines were permissive for efficient productive infection and spread. These results may prove useful in designing appropriate animal models to assess the in vivo infectivity properties of PERV.

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