Alteration of Cytokeratin 7 and Cytokeratin 20 Expression Profile Is Uniquely Associated With Tumorigenesis of Primary Adenocarcinoma of the Small Intestine

AimsTubular carcinoid is a rare variant of appendiceal well-differentiated neuroendocrine tumour. Although considered benign lesions, the small infiltrating tubules that characterise the tumour may raise concern for metastatic adenocarcinoma. To our knowledge, the cytokeratin 7 (CK7)/cytokeratin 20 (CK20) expression profile of these neoplasms remains unexplored.MethodsThe authors characterised the CK7/CK20 immunophenotype and Ki-67 expression of the eight available tubular carcinoids seen at their institution from 1991 to 2011.ResultsCK7 and CK20 staining was variable, ranging from none to focal staining for either or both CK7 and CK20, to diffuse expression of CK7 or CK20.ConclusionsThe CK7/CK20 expression profile is of limited value when the differential diagnosis includes primary tubular carcinoid and well-differentiated metastatic adenocarcinoma. In such cases, careful attention to the location of the neoplasm, mitotic count and presence or absence of an associated classic carcinoid component are more useful for arriving at the correct diagnosis.

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Clear Cell Ductal Adenocarcinoma of Pancreas: A Case Report and Review of the Literature

Archives of Pathology & Laboratory Medicine ◽

10.5858/2004-128-693-ccdaop ◽

2004 ◽

Vol 128 (6) ◽

pp. 693-696 ◽

Cited By ~ 2

Author(s):

Sourav Ray ◽

Zhengbin Lu ◽

Swaminathan Rajendiran

Keyword(s):

Clear Cell ◽

Clear Cell Carcinoma ◽

Ductal Adenocarcinoma ◽

Molecular Evidence ◽

Cytokeratin 20 ◽

Ras Oncogene ◽

Cytokeratin 7 ◽

Review Of The Literature ◽

Hyaline Globules ◽

Hmb 45

Abstract We present a unique carcinoma of the pancreas with predominantly clear cell morphology (>95% clear cells). Mucicarmine stain revealed abundant intraluminal and intracytoplasmic mucin. Immunohistochemically, the cells were positive for the epithelial markers cytokeratin 7 and CAM 5.2, and were focally positive for cytokeratin 20. These cells also expressed monoclonal carcinoembryonic antigen. Stains for the neuroendocrine markers synaptophysin and chromogranin were negative, as were stains for vimentin, p53, HMB-45, and CD10. An additional outstanding feature was the presence of dense intraluminal and intracytoplasmic hyaline globules, which were immunohistochemically positive for α1-antitrypsin. Sequencing of the K-ras oncogene revealed a point mutation in codon 12, providing molecular evidence of ductal origin. In the proper morphologic context supported by immunohistochemistry, clear cell carcinoma can be regarded as a rare variant of ductal adenocarcinoma.

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Cytokeratin 7 and Cytokeratin 20 in Primary Urinary Bladder Carcinoma and Matched Lymph Node Metastasis

Archives of Pathology & Laboratory Medicine ◽

10.5858/2001-125-0921-cacipu ◽

2001 ◽

Vol 125 (7) ◽

pp. 921-923 ◽

Cited By ~ 4

Author(s):

Jiazhong Jiang ◽

Thomas M. Ulbright ◽

Cheryl Younger ◽

Katya Sanchez ◽

David G. Bostwick ◽

...

Keyword(s):

Lymph Node ◽

Lymph Node Metastasis ◽

Urinary Bladder ◽

Urothelial Carcinoma ◽

Lymph Node Metastases ◽

Primary Tumor ◽

Cytokeratin 20 ◽

Cytokeratin 7 ◽

Node Metastasis ◽

Node Metastases

Abstract Background.—Cytokeratin 7 (CK7) and cytokeratin 20 (CK20) are 2 types of intermediate filament protein. Expression of CK7 is seen in the majority of primary urinary bladder carcinomas. CK20 is restricted to superficial and occasional intermediate cells of the normal urothelium of the bladder. Aberrant CK20 expression has been documented in urothelial carcinoma and has proved useful as an ancillary diagnostic aid for urinary bladder tumor. Our hypothesis is that the pattern of CK7 and CK20 expression in metastatic urothelial carcinoma duplicates the expression of the same markers in the primary tumors. Therefore, immunohistochemical staining of metastatic tumors for these 2 markers may be helpful for differential diagnosis in ambiguous metastatic tumor deposits. Objective.—To determine the concordance of CK7 and CK20 expression in primary bladder urothelial carcinoma and the matched lymph node metastasis. Design.—We studied 26 patients with lymph node metastases who underwent radical cystectomy and bilateral lymphadenectomy for bladder carcinoma. Immunohistochemical staining for CK7 and CK20 was performed on formalin-fixed paraffin-embedded tissues containing primary cancers and lymph node metastases. Results.—In all cases, there was a concordant expression of CK20 in the primary cancer and its matched lymph node metastasis. Twelve cases (46%) showed positive CK20 immunoreactivity in the primary tumor and its matched lymph node metastases, whereas 14 cases (54%) were negative for CK20 in both the primary tumor and lymph node metastasis. All cases showed positive CK7 immunoreactivity in the primary cancers and matched lymph node metastases. Conclusions.—CK20 immunoreactivity is reliably observed in metastases from bladder cancer when the primary tumor expresses CK20.

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Factors influencing long-term survival in 100 cases of small intestine primary adenocarcinoma

The American Journal of Surgery ◽

10.1016/s0002-9610(97)89599-2 ◽

1997 ◽

Vol 173 (3) ◽

pp. 237-239 ◽

Cited By ~ 40

Author(s):

Michel Veyrières ◽

Patrice Baillet ◽

Jean Marie Hay ◽

Abe Fingerhut ◽

Jean Luc Bouillot ◽

...

Keyword(s):

Small Intestine ◽

Primary Adenocarcinoma ◽

Term Survival ◽

Long Term Survival ◽

Factors Influencing

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Immunoreactivity of cytokeratins 7 and 20 in goblet cells and columnar blue cells in patients with endoscopic evidence of Barrett's esophagus

Arquivos de Gastroenterologia ◽

10.1590/s0004-28032009000200010 ◽

2009 ◽

Vol 46 (2) ◽

pp. 127-131 ◽

Cited By ~ 1

Author(s):

João Carlos Cantarelli Jr. ◽

Renato Borges Fagundes ◽

Luise Meurer ◽

Marta Pires da Rocha ◽

André Nicola ◽

...

Keyword(s):

Intestinal Metaplasia ◽

Barrett’S Esophagus ◽

Alcian Blue ◽

Barrett's Esophagus ◽

Immunohistochemical Staining ◽

Esophagogastric Junction ◽

Cell Types ◽

Goblet Cells ◽

Cytokeratin 20 ◽

Cytokeratin 7

CONTEXT: Barrett's esophagus is characterized by the presence of goblet cells. However, when alcian-blue is utilized, another type of cells, called columnar blue cells, is frequently present in the distal esophagus of patients with endoscopic evidence of Barrett's esophagus. Cytokeratin 7 and 20 immunoreactivity has been previously studied in areas of intestinal metaplasia at the esophagogastric junction. However, the expression of these cytokeratins in columnar blue cells has not been characterized. OBJECTIVE: To compare the expression of cytokeratin 7 and 20 in goblet cells and columnar blue cells in patients with endoscopic evidence of Barrett's esophagus. METHODS: Biopsies from 86 patients with endoscopic evidence of Barrett's esophagus were evaluated. The biopsies were stained for cytokeratin 7 and 20. RESULTS: Goblet cells were present in 75 cases and columnar blue cells in 50 cases. Overall, cytokeratin 7 expression was similar in goblet cells and columnar blue cells (P = 0.25), while cytokeratin 20 was more common in goblet cells (P <0.001). In individuals with both cell types, however, cytokeratin 7 staining was the same in goblet and columnar blue cells in 95% of the cases, and cytokeratin 20 staining was the same in 77%. CONCLUSION: Goblet cells and columnar blue cells have similar immunohistochemical staining patterns for cytokeratins 7 and 20 in patients with endoscopic evidence of Barrett's esophagus.

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A submandibular nodule revealing a relapsed bladder urothelial carcinoma

Our Dermatology Online ◽

10.7241/ourd.2021e.10 ◽

2021 ◽

Vol 12 (e) ◽

pp. e10-e10

Author(s):

Aya Khemir ◽

Nada Mansouri ◽

Faten Gargouri ◽

Fethi Bougrine

Keyword(s):

Bladder Cancer ◽

Physical Examination ◽

Urothelial Carcinoma ◽

Medical History ◽

Tumor Cells ◽

Microscopic Examination ◽

Survival Rates ◽

Cytokeratin 20 ◽

Cytokeratin 7 ◽

Total Recovery

A 69-year-old patient treated for infiltrating bladder transitional carcinoma many years ago presented with a submandibular nodule. The last was fortuitously discovered by the patient a month before he presented to consultation. Physical examination showed a firm subcutaneous nodule of 0.5 cm in diameter in the right submandibular region. At this level skin was inflamed/red and swollen. Otherwise physical examination was within normal. The described nodule above was biopsied. Microscopic examination showed infiltration of the dermis by a carcinomatous proliferation (Fig. 1). Tumor cells were arranged in small nests and clusters surrounded by a fibrous stroma. Tumor cells showed moderate nuclear atypia. Immunohistochemical staining showed positivity of tumor cells for Cytokeratin 7 and P63 (Fig. 2). Therefore, taking into consideration patient’s medical history, microscopic and immunohistochemical findings the diagnosis of CM from urothelial carcinoma was retained. The first case of CM from bladder carcinoma was reported in 1909 [3]. Since then many case have been reported [1,2]. According to cases reported in literature so far, the mean interval of time between the setting of bladder cancer and the appearance of CM is of 18 months approximately. Large tumor size and deep infiltration of the bladder wall are predictive factors of CM. However, cases of CM associated with superficial bladder carcinomas were reported [3]. The certain diagnosis is based on microscopic examination [1,3]. Pathologists should be aware of patient’s medical history to facilitate the diagnosis and choosing appropriate immunostains if necessary especially in front of a poorly differentiated carcinoma[3]. Urothelial carcinomas express Cytokeratin 7 and Cytokeratin 20 antibodies [3]. The occurring of CM in case of bladder cancer darken the prognosis [1,2]. Median survival rates are less than 12 months in published cases so far [1,3]. Treatment consists of chemotherapy if the patient could bare it [1]. Total recovery was detected in 70% of cases of CM treated with chemotherapy. Yet, it does not improve global survival rates [3].

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Gene expression profile data of the developing small intestine of Id2-deficient mice

Data in Brief ◽

10.1016/j.dib.2019.103717 ◽

2019 ◽

Vol 24 ◽

pp. 103717

Author(s):

Kentaro Mori ◽

Kota Tamada ◽

Hisanori Kurooka ◽

Makoto Matsui ◽

Toru Takumi ◽

...

Keyword(s):

Gene Expression ◽

Small Intestine ◽

Gene Expression Profile ◽

Expression Profile ◽

Profile Data ◽

Gene Expression Profile Data ◽

Deficient Mice

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Primary adenocarcinoma of the small intestine

The American Journal of Surgery ◽

10.1016/0002-9610(77)90399-3 ◽

1977 ◽

Vol 134 (3) ◽

pp. 331-333 ◽

Cited By ~ 28

Author(s):

David F. Morgan ◽

Ronald W. Busuttil

Keyword(s):

Small Intestine ◽

Primary Adenocarcinoma

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Immunohistochemical Detection of Uroplakin III, Cytokeratin 7, and Cytokeratin 20 in Canine Urothelial Tumors

Veterinary Pathology ◽

10.1354/vp.40-1-55 ◽

2003 ◽

Vol 40 (1) ◽

pp. 55-62 ◽

Cited By ~ 54

Author(s):

J. A. Ramos-Vara ◽

M. A. Miller ◽

M. Boucher ◽

A. Roudabush ◽

G. C. Johnson

Keyword(s):

Urinary Bladder ◽

Transitional Cell ◽

Cytokeratin 20 ◽

Cytokeratin 7 ◽

Bladder Tumors ◽

Cytoplasmic Staining ◽

Negative Results ◽

Urothelial Tumors ◽

Carcinoma Of The Bladder ◽

Intermediate Cells

Immunohistochemistry for uroplakin III (UP III), cytokeratin 7 (CK 7), and cytokeratin 20 (CK 20) using commercially available antibodies was done in normal canine urinary bladder and 72 canine urinary bladder tumors that had been fixed in formalin and embedded in paraffin. Prolonged fixation (3–28 days) did not significantly alter the immunostaining for UP III. There was moderate reduction in the intensity for CK 7 and CK 20 after 1 week of fixation. UP III was detected in superficial (umbrella) cells and some intermediate cells of the normal urinary bladder, 7 of 7 transitional cell papillomas (TCPs), 50 of 55 transitional cell carcinomas (TCCs), and 4 of 5 metastatic TCCs. Staining was typically outlined in the plasma membrane, but diffuse or focal cytoplasmic staining was also observed. Intracytoplasmic lumina were usually positive for UP III. One squamous cell carcinoma of the bladder, 4 nonepithelial bladder tumors, and 285 nonurothelial tumors from different nonurinary locations were negative for UP III. CK 7 was detected in 7 of 7 TCPs, 53 of 54 TCCs, and 5 of 5 metastatic TCCs. The staining for CK 7 was diffuse cytoplasmic. CK 20 was detected in 1 of 7 TCPs, 37 of 54 TCCs, and 1 of 5 metastatic TCCs. The staining with CK 20 was cytoplasmic and weaker than with antibodies to UP III or CK 7. There was concurrent expression of UP III, CK 7, and CK 20 in 36 of 54 TCCs. UP III is a specific and sensitive marker for canine transitional epithelial (urothelial) neoplasms, detecting 91% of TCCs. Negative results may be observed with anaplastic tumors.

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