LP14: HYPOXIA-INDUCIBLE FACTOR 1 (HIF-1A) EXPRESSION AS AN INDICATOR FOR HYPOXIA IN ENDOTHELIAL PROGENITOR CELLS (EPC) AND THE BIOARTIFICIAL TISSUE WITHIN THE ARTERIO-VENOUS (AV) LOOP RAT ISOLATION CHAMBER

2010 ◽  
Vol 126 (2) ◽  
pp. 719
Author(s):  
O. Bleiziffer ◽  
Q. Yuan ◽  
H. Seuss ◽  
A. Arkudas ◽  
J. Beier ◽  
...  
2016 ◽  
Vol 105 ◽  
pp. 85-92 ◽  
Author(s):  
Christian Kütscher ◽  
Florian M. Lampert ◽  
Mirjam Kunze ◽  
Filiz Markfeld-Erol ◽  
G. Björn Stark ◽  
...  

2022 ◽  
Vol 12 (4) ◽  
pp. 731-738
Author(s):  
Zhitang Chang ◽  
Guotai Sheng ◽  
Yizhong Zhou ◽  
Zhiyong Wu ◽  
Guobo Xie ◽  
...  

Based on the promotion of myocardial activity via endothelial progenitor cells (EPCs) subsequent to acute myocardial infarction (AMI), our research was designed to explore the influence of excessive HIF-1α expression in expanded EPCs (eEPCs) on promotion of the activity of left ventricle subsequent to MI. Isolation of EPCs from cord blood was performed before transduction with the help of retroviral vector with or without HIF-1α expression. Transplantation was performed subsequent to ligation of the left anterior descending coronary artery in mice. Ejection fraction (EF) of left ventricle was promoted via transplantation after 2 weeks. Excessive HIF-1α expression enhanced EF of left ventricle and decreased the extent of MI. It was revealed via functional studies that excessive HIF-1α expression enhanced proliferation of EPCs triggered by low oxygen concentration and suppressed cell death in the region of infarction. Moreover, markers of endothelium CD31, VEGF, and eNOS were upregulated. Transplantation of eEPCs with excessive HIF-1α expression in AMI can promote myocardial activities by increasing differentiation, generation of vessels, proliferation of eEPCs, and suppressing cell death. The above findings propose that regulation of EPCs via HIF-1α enhances the activity as well as mobilization of EPCs, indicating that reinforcement of expression of HIF-1α is beneficial for coronary heart disease.


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