scholarly journals 132 Characterization of 2 Epithelial Cell Air-Liquid Interface (ALI) Culture Models for Human Healthy Nasal Mucosa and Nasal Polyps

2012 ◽  
Vol 5 ◽  
pp. S44
Author(s):  
Fco de Borja Callejas ◽  
Asunción Martínez-Antón ◽  
Jordi Roca-Ferrer ◽  
Julio Cortijo ◽  
César Picado ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Nasal Mucosa ◽  
Nasal Polyps ◽  
Liquid Interface ◽  
Culture Models ◽  
Air Liquid Interface

Characterization of biofilm formation by Salmonella enterica at the air-liquid interface in aquatic environments

2018 ◽  
Vol 190 (4) ◽  
Author(s):  
José Andrés Medrano-Félix ◽  
Cristóbal Chaidez ◽  
Kristina D. Mena ◽  
María del Socorro Soto-Galindo ◽  
Nohelia Castro-del Campo
Keyword(s):  
Salmonella Enterica ◽  
Biofilm Formation ◽  
Liquid Interface ◽  
Aquatic Environments ◽  
Air Liquid Interface

Passive siRNA transfection method for gene knockdown in air-liquid interface airway epithelial cell cultures

2021 ◽  
Author(s):  
Colleen M Bartman ◽  
Kimberly E Stelzig ◽  
David R Linden ◽  
Y. S. Prakash ◽  
Sergio E Chiarella
Keyword(s):  
Epithelial Cell ◽  
Liquid Interface ◽  
Airway Epithelial ◽  
Loss Of Function ◽  
Sirna Transfection ◽  
Simple Method ◽  
Primary Epithelial Cell ◽  
Transfection Protocol ◽  
Air Liquid Interface

Differentiation of human bronchial epithelial cells (HBEs) in air-liquid interface (ALI) cultures recapitulates organotypic modeling of the in vivo environment. Although ALI cultures are invaluable for studying the respiratory epithelial barrier, loss-of-function studies are limited by potentially cytotoxic reagents in classical transfection methods, the length of the differentiation protocol, and the number of primary epithelial cell passages. Here, we present the efficacy and utility of a simple method for siRNA transfection of HBEs in ALI cultures that does not require potentially cytotoxic transfection reagents and does not detrimentally alter the physiology of HBEs during the differentiation process. This transfection protocol introduces a reproducible and efficient method for loss-of-function studies in HBE ALI cultures that can be leveraged for modeling the respiratory system and airway diseases.

ChemInform Abstract: Design and Characterization of Crystalline Thin Film Architectures at the Air-Liquid Interface: Simplicity to Complexity

ChemInform ◽  
2010 ◽  
Vol 32 (33) ◽  
pp. no-no
Author(s):  
Ivan Kuzmenko ◽  
Hanna Rapaport ◽  
Kristian Kjaer ◽  
Jens Als-Nielsen ◽  
Isabelle Weissbuch ◽  
...  
Keyword(s):  
Thin Film ◽  
Liquid Interface ◽  
Air Liquid Interface

scholarly journals Dosing intact birch pollen grains at the air-liquid interface (ALI) to the immortalized human bronchial epithelial cell line BEAS-2B

PLoS ONE ◽  
2021 ◽  
Vol 16 (11) ◽  
pp. e0259914
Author(s):  
Joana Candeias ◽  
Carsten B. Schmidt-Weber ◽  
Jeroen Buters
Keyword(s):  
Epithelial Cell ◽  
Cell Line ◽  
Pollen Grains ◽  
Bronchial Epithelial Cell ◽  
Liquid Interface ◽  
Epithelial Cell Line ◽  
Single Particles ◽  
Bronchial Epithelial ◽  
Pollen Exposure ◽  
Air Liquid Interface

In real life, humans are exposed to whole pollen grains at the air epithelial barrier. We developed a system for in vitro dosing of whole pollen grains at the Air-Liquid Interface (ALI) and studied their effect on the immortalized human bronchial epithelial cell line BEAS-2B. Pollen are sticky and large particles. Dosing pollen needs resuspension of single particles rather than clusters, and subsequent transportation to the cells with little loss to the walls of the instrumentation i.e. in a straight line. To avoid high speed impacting insults to cells we chose sedimentation by gravity as a delivery step. Pollen was resuspended into single particles by pressured air. A pollen dispersion unit including PTFE coating of the walls and reduced air pressure limited impaction loss to the walls. The loss of pollen to the system was still about 40%. A linear dose effect curve resulted in 327-2834 pollen/cm2 (± 6.1%), the latter concentration being calculated as the amount deposited on epithelial cells on high pollen days. After whole pollen exposure, the largest differential gene expression at the transcriptomic level was late, about 7 hours after exposure. Inflammatory and response to stimulus related genes were up-regulated. We developed a whole pollen exposure air-liquid interface system (Pollen-ALI), in which cells can be gently and reliably dosed.

scholarly journals Functional and Structural Characterization of a Lyophilized Pulmonary Surfactant Directly Applied onto the Air-Liquid Interface

2020 ◽  
Vol 118 (3) ◽  
pp. 388a
Author(s):  
Mercedes Echaide ◽  
Sonia Vazquez-Sanchez ◽  
Antonio Cruz ◽  
Jesus Perez-Gil
Keyword(s):  
Structural Characterization ◽  
Pulmonary Surfactant ◽  
Liquid Interface ◽  
Air Liquid Interface
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