Characterization of biofilm formation by Salmonella enterica at the air-liquid interface in aquatic environments

2018 ◽  
Vol 190 (4) ◽  
Author(s):  
José Andrés Medrano-Félix ◽  
Cristóbal Chaidez ◽  
Kristina D. Mena ◽  
María del Socorro Soto-Galindo ◽  
Nohelia Castro-del Campo
Keyword(s):  
Salmonella Enterica ◽  
Biofilm Formation ◽  
Liquid Interface ◽  
Aquatic Environments ◽  
Air Liquid Interface

scholarly journals Role ofCaulobacterCell Surface Structures in Colonization of the Air-Liquid Interface

2019 ◽  
Vol 201 (18) ◽  
Author(s):  
Aretha Fiebig
Keyword(s):  
Cell Surface ◽  
Caulobacter Crescentus ◽  
Three Dimensional ◽  
Liquid Interface ◽  
Type Iv Pili ◽  
Surface Structures ◽  
Aquatic Environments ◽  
Content Type ◽  
Type Iv ◽  
Air Liquid Interface

ABSTRACTIn aquatic environments,Caulobacterspp. can be found at the boundary between liquid and air known as the neuston. I report an approach to study temporal features ofCaulobacter crescentuscolonization and pellicle biofilm development at the air-liquid interface and have defined the role of cell surface structures in this process. At this interface,C. crescentusinitially forms a monolayer of cells bearing a surface adhesin known as the holdfast. When excised from the liquid surface, this monolayer strongly adheres to glass. The monolayer subsequently develops into a three-dimensional structure that is highly enriched in clusters of stalked cells known as rosettes. As this pellicle film matures, it becomes more cohesive and less adherent to a glass surface. A mutant strain lacking a flagellum does not efficiently reach the surface, and strains lacking type IV pili exhibit defects in organization of the three-dimensional pellicle. Strains unable to synthesize the holdfast fail to accumulate at the boundary between air and liquid and do not form a pellicle. Phase-contrast images support a model whereby the holdfast functions to trapC. crescentuscells at the air-liquid boundary. Unlike the holdfast, neither the flagellum nor type IV pili are required forC. crescentusto partition to the air-liquid interface. While it is well established that the holdfast enables adherence to solid surfaces, this study provides evidence that the holdfast has physicochemical properties that allow partitioning of nonmotile mother cells to the air-liquid interface and facilitate colonization of this microenvironment.IMPORTANCEIn aquatic environments, the boundary at the air interface is often highly enriched with nutrients and oxygen. Colonization of this niche likely confers a significant fitness advantage in many cases. This study provides evidence that the cell surface adhesin known as a holdfast enablesCaulobacter crescentusto partition to and colonize the air-liquid interface. Additional surface structures, including the flagellum and type IV pili, are important determinants of colonization and biofilm formation at this boundary. Considering that holdfast-like adhesins are broadly conserved inCaulobacterspp. and other members of the diverse classAlphaproteobacteria, these surface structures may function broadly to facilitate colonization of air-liquid boundaries in a range of ecological contexts, including freshwater, marine, and soil ecosystems.

ChemInform Abstract: Design and Characterization of Crystalline Thin Film Architectures at the Air-Liquid Interface: Simplicity to Complexity

ChemInform ◽  
2010 ◽  
Vol 32 (33) ◽  
pp. no-no
Author(s):  
Ivan Kuzmenko ◽  
Hanna Rapaport ◽  
Kristian Kjaer ◽  
Jens Als-Nielsen ◽  
Isabelle Weissbuch ◽  
...  
Keyword(s):  
Thin Film ◽  
Liquid Interface ◽  
Air Liquid Interface

scholarly journals Functional and Structural Characterization of a Lyophilized Pulmonary Surfactant Directly Applied onto the Air-Liquid Interface

2020 ◽  
Vol 118 (3) ◽  
pp. 388a
Author(s):  
Mercedes Echaide ◽  
Sonia Vazquez-Sanchez ◽  
Antonio Cruz ◽  
Jesus Perez-Gil
Keyword(s):  
Structural Characterization ◽  
Pulmonary Surfactant ◽  
Liquid Interface ◽  
Air Liquid Interface

1708 Characterization of Air-Liquid Interface in Horizontal Duct Based on Visualization of Liquid Film

2015 ◽  
Vol 2015 (0) ◽  
pp. _1708-1_-_1708-3_
Author(s):  
Daichi TANAKA ◽  
Yoichi OGATA ◽  
Keiya NISHIDA ◽  
Ryo YAMAMOTO ◽  
Kazuhiro NAKAMURA ◽  
...  
Keyword(s):  
Liquid Film ◽  
Liquid Interface ◽  
Air Liquid Interface

scholarly journals Disruption of Escherichia coli Amyloid-Integrated Biofilm Formation at the Air–Liquid Interface by a Polysorbate Surfactant

Langmuir ◽  
2013 ◽  
Vol 29 (3) ◽  
pp. 920-926 ◽  
Author(s):  
Cynthia Wu ◽  
Ji Youn Lim ◽  
Gerald G. Fuller ◽  
Lynette Cegelski
Keyword(s):  
Escherichia Coli ◽  
Biofilm Formation ◽  
Liquid Interface ◽  
Air Liquid Interface

scholarly journals 132 Characterization of 2 Epithelial Cell Air-Liquid Interface (ALI) Culture Models for Human Healthy Nasal Mucosa and Nasal Polyps

2012 ◽  
Vol 5 ◽  
pp. S44
Author(s):  
Fco de Borja Callejas ◽  
Asunción Martínez-Antón ◽  
Jordi Roca-Ferrer ◽  
Julio Cortijo ◽  
César Picado ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Nasal Mucosa ◽  
Nasal Polyps ◽  
Liquid Interface ◽  
Culture Models ◽  
Air Liquid Interface

scholarly journals Extending an Eco-Evolutionary Understanding of Biofilm-Formation at the Air-Liquid Interface to Community Biofilms

2020 ◽  
Author(s):  
Robyn Jerdan ◽  
Olga Iungin ◽  
Olena V. Moshynets ◽  
Geert Potters ◽  
Andrew J. Spiers
Keyword(s):  
Biofilm Formation ◽  
Liquid Interface ◽  
Air Liquid Interface

scholarly journals In vitro hazard characterization of simulated aircraft cabin bleed-air contamination in lung models using an air-liquid interface (ALI) exposure system

2021 ◽  
Vol 156 ◽  
pp. 106718
Author(s):  
Rui-Wen He ◽  
Marc M.G. Houtzager ◽  
W.P. Jongeneel ◽  
Remco H.S. Westerink ◽  
Flemming R. Cassee
Keyword(s):  
Liquid Interface ◽  
Exposure System ◽  
Air Contamination ◽  
Aircraft Cabin ◽  
Hazard Characterization ◽  
Air Liquid Interface
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