Flow cytometry analysis of cellular DNA content from formalin fixed paraffin embedded tissues: a useful technique in barrett’s surveillance?

Pathology ◽  
2010 ◽  
Vol 42 ◽  
pp. S61
Author(s):  
Suad Al Jahdami ◽  
Sabine Gruninger ◽  
Rose Miller ◽  
Raylin DeJesus ◽  
Bruce Shadbolt ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Dna Content ◽  
Flow Cytometry Analysis ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Cellular Dna ◽  
Barrett’S Surveillance ◽  
Formalin Fixed

Intratumoral DNA content heterogeneity in breast carcinomas demonstrated by core punch tissue sampling and flow cytometry

2014 ◽  
Vol 67 (9) ◽  
pp. 821-824 ◽  
Author(s):  
Zhouwei Zhang ◽  
Donald L Weaver ◽  
Kavita Munjal ◽  
Mark F Evans
Keyword(s):  
Flow Cytometry ◽  
Dna Content ◽  
Intratumoral Heterogeneity ◽  
Dna Index ◽  
Surgical Specimen ◽  
Flow Cytometric ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Relationship Of ◽  
Formalin Fixed

Further to advancements in instrumentation and fluorescent dye technologies, there has been a resurgence of interest in the flow cytometric assay of formalin-fixed, paraffin-embedded specimens. Here we present a novel, simple and effective alternative to whole block sectioning that allows selective multisampling of tissues within a specimen block and the investigation of intratumoral heterogeneity. Formalin-fixed, paraffin-embedded breast carcinoma specimens were core-punched using 1.0 mm diameter needles and assayed by flow cytometry using a modified Hedley method. Intratumoral heterogeneity for DNA index and per cent S-phase fraction was detected in 10 of 23 (44%) and 11 of 23 (47%) specimens respectively. Macro-level genomic heterogeneity is common in breast cancer even within a single surgical specimen block. Studies investigating the relationship of DNA content heterogeneity to other markers of genomic instability such as mutations, deletions, insertions and translocations are warranted.

Assessment of DNA content in formalin-fixed, paraffin-embedded tissue of lung cancer by laser scanning cytometer

1999 ◽  
Vol 49 (8) ◽  
pp. 695-701 ◽  
Author(s):  
Noriki Kamiya ◽  
Tomoyuki Yokose ◽  
Yoshimasa Kiyomatsu ◽  
Michael T Fahey ◽  
Tetsuro Kodama ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Dna Content ◽  
Laser Scanning ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Laser Scanning Cytometer ◽  
Formalin Fixed

scholarly journals Improved dna content histograms from formalin-fixed, paraffin-embedded liver tissue by proteinase K digestion

Cytometry ◽  
1993 ◽  
Vol 14 (6) ◽  
pp. 673-678 ◽  
Author(s):  
James Albro ◽  
Kenneth D. Bauer ◽  
Charles L. Hitchcock ◽  
Carl T. Wittwer
Keyword(s):  
Dna Content ◽  
Liver Tissue ◽  
Proteinase K ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Formalin Fixed
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