Isobaric Tags for Relative and Absolute Quantitation–Based Proteomic Analysis of Patent and Constricted Ductus Arteriosus Tissues Confirms the Systemic Regulation of Ductus Arteriosus Closure

2015 ◽  
Vol 66 (2) ◽  
pp. 204-213 ◽  
Author(s):  
Haifa Hong ◽  
Lincai Ye ◽  
Huiwen Chen ◽  
Yu Xia ◽  
Yue Liu ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Ductus Arteriosus ◽  
Absolute Quantitation ◽  
Isobaric Tags

scholarly journals Proteomic Analysis of Beef Tenderloin and Flank Assessed Using an Isobaric Tag for Relative and Absolute Quantitation (iTRAQ)

Animals ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 150
Author(s):  
Zhaomin Lei ◽  
Jianping Wu ◽  
Deyin Zhang ◽  
Ting Liu ◽  
Shengguo Zhao ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Expression Patterns ◽  
Tca Cycle ◽  
Absolute Quantification ◽  
Absolute Quantitation ◽  
Oxidation Reduction ◽  
The Tca Cycle ◽  
Isobaric Tag ◽  
Isobaric Tags

Herein, we performed a proteomic analysis of tenderloin and flank steaks from Simmental cattle using the isobaric tags for a relative and absolute quantification (iTRAQ) approach. We identified 17 amino acids in both steaks, and Gly, Cys, Ile, Lys, and Pro differed most in abundance between the steak types (p < 0.05). A comparison of the expression patterns in steaks revealed 128 differentially expressed proteins (DEPs), of which 44 were up-regulated and 84 were down-regulated. Furthermore, 27 DEPs (p < 0.05) were subjected to gene ontology (GO) analysis, and many were found to be related to oxidation-reduction, metabolism, hydrogen ion transmembrane transport, transport, the tricarboxylic acid (TCA) cycle, mitochondrial electron transport, and the conversion of nicotinamide adenine dinucleotide (NADH) to ubiquinone. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis also implicated these DEPs in various signalling pathways, including oxidative phosphorylation, cardiac muscle contraction, the TCA cycle, biosynthesis, and the metabolism. These findings provide a new insight into key proteins involved in the determination of amino acid composition in beef.

iTRAQ-based quantitative proteomic analysis of two transgenic soybean lines and the corresponding non-genetically modified isogenic variety

2019 ◽  
Vol 167 (1) ◽  
pp. 67-78
Author(s):  
Weixiao Liu ◽  
Zhe Zhang ◽  
Xuri Liu ◽  
Wujun Jin
Keyword(s):  
Proteomic Analysis ◽  
Interaction Analysis ◽  
Genetically Modified ◽  
Unintended Effects ◽  
Post Translational Modification ◽  
Absolute Quantitation ◽  
Protein Protein Interaction ◽  
Transduction Mechanisms ◽  
Isobaric Tags ◽  
Gm Soybean

Abstract To investigate the unintended effects of genetically modified (GM) crops, an isobaric tags for relative and absolute quantitation (iTRAQ)-based comparative proteomic analysis was performed with seed cotyledons of two GM soybean lines, MON87705 and MON87701×MON89788, and the corresponding non-transgenic isogenic variety A3525. Thirty-five differentially abundant proteins (DAPs) were identified in MON87705/A3525, 27 of which were upregulated and 8 downregulated. Thirty-eight DAPs were identified from the MON87701×MON89788/A3525 sample, including 29 upregulated proteins and 9 downregulated proteins. Pathway analysis showed that most of these DAPs participate in protein processing in endoplasmic reticulum and in metabolic pathways. Protein–protein interaction analysis of these DAPs demonstrated that the main interacting proteins are associated with post-translational modification, protein turnover, chaperones and signal transduction mechanisms. Nevertheless, these DAPs were not identified as new unintended toxins or allergens and only showed changes in abundance. All these results suggest that the seed cotyledon proteomic profiles of the two GM soybean lines studied were not dramatically altered compared with that of their natural isogenic control.

scholarly journals Isobaric Tags for Relative and Absolute Quantitation (iTRAQ)-Based  proteomic analysis of mRNA splicing relevant proteins in aging HSPCs

2019 ◽  
Author(s):  
Xiaolan Lian ◽  
Lina Zhang
Keyword(s):  
Proteomic Analysis ◽  
Aging Process ◽  
Mrna Splicing ◽  
Absolute Quantitation ◽  
Qrt Pcr ◽  
Isobaric Tags ◽  
Spliceostatin A

Abstract Background: HSPC aging was closely associated with the organism aging, senile diseases and hematopoietic related diseases. Therefore, study on HSPC aging born great significance to further elucidate the mechanisms of aging and to treat hematopoietic disease resulted from HSPC aging. Little attention had been paid to mRNA splicing as a mechanism underlying HSPC senescence. Results: We used our lab’s patented aging model of HSPCs in vitro to analyze mRNA splicing relevant proteins alterations with iTRAQ based proteomic analysis. We found that not only the notable mRNA splicing genes such as SR, hnRNP, WBP11, Sf3b1, Ptbp1 and U2AF1 but also the scarcely reported mRNA splicing relavant genes such as Rbmxl1, Dhx16, Pcbp2, Pabpc1 were significantly down-regulated. We further verified their genes expressions by qRT-PCR. In addition, we reported the effect of Spliceostatin A (SSA), which inhibits mRNA splicing in vivo and in vitro, on HSPC aging. Conclusions: It was concluded that mRNA splicing emerged as an important vulnerability of HSPC aging. This study improved our understanding of the role of mRNA splicing in the HSPC aging process.

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