Implantation of an Artificial Endothelial Layer for Treatment of Chronic Corneal Edema

Cornea ◽  
2021 ◽  
Vol Publish Ahead of Print ◽  
Author(s):  
Gerd U. Auffarth ◽  
Hyeck-Soo Son ◽  
Matthias Koch ◽  
Jan Weindler ◽  
Patrick Merz ◽  
...  
Keyword(s):  
Corneal Edema ◽  
Endothelial Layer

scholarly journals The Effect of Bioregulators Isolated from Blood Serum and Cornea of the Bovin’s Eye on the Condition of Tissue and Cells of the Rabbit Corneas during Cultivation and Storage

2021 ◽  
Vol 18 (3) ◽  
pp. 488-494
Author(s):  
M. S. Krasnov ◽  
V. P. Yamskova
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Blood Serum ◽  
Corneal Edema ◽  
Control Group ◽  
Endothelial Layer ◽  
Epidermal Growth ◽  
Immunohistochemical Studies ◽  
Control Samples

Objective: to study the condition of the cornea, as well as its epithelial and endothelial cells, while maintaining in vitro at various temperature conditions, under the influence of a number of factors, including bioregulators isolated from blood serum and cornea of the bovine, and epidermal growth factor.Methods. The study was carried out on rabbit corneas stored at temperatures of +4, –86 °C, as well as the cultivation of endothelial and epithelial cells isolated from the cornea after storage at these temperatures, followed by histological and immunohistochemical studies.Results. Storage of the cornea at +4 °C for 10 days leads to corneal edema and significantly reduces their transparency, both bioregulators partially prevent a decrease in the transparency of the cornea, while the endothelial layer lyses in groups with the addition of epidermal growth factor and corneal bioregulator; but remains in the cornea with the addition of a serum bioregulator. All three factors contribute to the preservation of the Bowman membrane. In the corneas stored at –86 °C on the 30th day, a preserved endothelial layer was observed, and the epithelium retained its multilayering in all groups with the addition of factors other than the control group. In the control samples, the epithelial layer partially exfoliated, the endothelial layer was almost completely lysed. Both bioregulators stimulated the proliferation of cells isolated from the native cornea and enhanced the action of the epidermal growth factor. Similar results were obtained on cells isolated from stored corneas for 2 weeks at –86 °C. In the case of combined use of the epidermal growth factor and bioregulators on the 30th day, the endothelial layer was mainly preserved, the Descemet’s membrane was not broken. In the control samples, the epithelium was mainly single-layered, partially exfoliated, and the endothelial layer was completely lysed.Conclusion. Storage of cornea during hypothermia (+4 °С) does not provide corneal viability for longer than 10 days. Storage under conditions of cryopreservation (–86 °C) ensures the viability of the cornea for 60 days. Adding bioregulators and an epidermal growth factor to the basic preservation medium allows one to obtain a structurally safe and viable cornea, while all cellular layers of the cornea, including the endothelial layer, are preserved and viable.

STUDY CLINICAL FEATURE AND RESULT OF TREATMENT PHACOMORPHIC GLAUCOMA

2015 ◽  
pp. 71-75
Author(s):  
Van Nam Phan ◽  
Ba Ken Tran
Keyword(s):  
Visual Acuity ◽  
Intraocular Pressure ◽  
Statistical Significance ◽  
Corneal Edema ◽  
Clinical Feature ◽  
Anterior Chamber Angle ◽  
Male Population ◽  
Significant Difference ◽  
Operative Complications ◽  
Before And After

Purpose: Study clinical feature of phacomorphic glaucoma. To evaluate the result of treatment phacomorphic glaucoma. Method: The retrospective, interventional study on 36 cases with phacomorphic glaucoma who underwent treated at Hue Central Hospital from 6/2010 to 6/2011. Standard of research: visual, IOP, before and after surgery, accompanying lesions and post-operative complications. The surgery is considered successful when postoperative IOP less than 21 mmHg. Results: Age 50-59 presented 30.5 percent, ≥ 60 presented 91.7 percent. There was a slight female preponderance (66.7%) compared to the male population (33.3%) which implies a statistically marginally significant difference. However there was no statistical significance difference when compared by the two subgroups. Patient in country presented 61,1% and city presented 38,9%. The duration between the onset of pain and surgery from 0 to < 5 days (77.8%), from 6 to 10 (16.7%) and >10 days presented 5.5%. The preoperative intraocular pressure 35 to 45mmHg (47.2%), 46-55 (30.6%), 56-65 (13.9%) and more than 65 presented 8.3%. The visual acuity preoperation less than 1metre count finger (94.5%), less than 3 metre count finger presented 5.5%. Close anterior chamber angle presented 80.6% and shallow was presented 19.4%. Corneal edema presented 100%, iritis presented 94.4%, dilated pupil larger 5mm presented 83.3%, Synchynea iris and cataract presented 72.2%. ECCE, implantation IOL combined trabeculectomy presented 11.1%, Phaco, implantation IOL combined trabeculectomy presented 69,5%, ECCE implantation IOL presented 5.6%, Phaco, implantation IOL presented 13.8%. Postoperative visual acuity from 1/10 to 5/10 presented 72.2%, no case have VA larger than 5/10. Postoperative 3 months VA 1/10 to 5/10 presented 72.2%, larger VA 5/10 presented 8.3%. Postoperative 3 months intraocular pressure ≤ 21mmHg presented 91.7%, 22 to 24mmHg presented 8.3%, no case have IOP ≥25mmHg. Postoperative edema presented 58.3%, iritis presented 58.3%. Key words: phacomorphic Glaucoma

Engineering interconnected 3D vascular networks in hydrogels using molded sodium alginate lattice as the sacrificial template

Lab on a Chip ◽  
2014 ◽  
Vol 14 (15) ◽  
pp. 2709-2716 ◽  
Author(s):  
Xue-Ying Wang ◽  
Zi-He Jin ◽  
Bo-Wen Gan ◽  
Song-Wei Lv ◽  
Min Xie ◽  
...  
Keyword(s):  
Sodium Alginate ◽  
Vascular Networks ◽  
Endothelial Layer ◽  
Sacrificial Template ◽  
Size And Morphology

We engineer interconnected 3D vascular networks in hydrogels using molded sodium alginate lattice as sacrificial templates. The size and morphology of simulated vascular networks were well controlled and a fully-developed endothelial layer was formed.

scholarly journals Paracellular and Transcellular Leukocytes Diapedesis Are Divergent but Interconnected Evolutionary Events

Genes ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 254
Author(s):  
Michel-Edwar Mickael ◽  
Norwin Kubick ◽  
Pavel Klimovich ◽  
Patrick Henckell Flournoy ◽  
Irmina Bieńkowska ◽  
...  
Keyword(s):  
Immune Cell ◽  
Critical Role ◽  
Cellular Polarity ◽  
Endothelial Layer ◽  
Trichoplax Adhaerens ◽  
Migration Pathway ◽  
Two Systems ◽  
Sequence Reconstruction ◽  
Rhoa Protein ◽  
Migration Pathways

Infiltration of the endothelial layer of the blood-brain barrier by leukocytes plays a critical role in health and disease. When passing through the endothelial layer during the diapedesis process lymphocytes can either follow a paracellular route or a transcellular one. There is a debate whether these two processes constitute one mechanism, or they form two evolutionary distinct migration pathways. We used artificial intelligence, phylogenetic analysis, HH search, ancestor sequence reconstruction to investigate further this intriguing question. We found that the two systems share several ancient components, such as RhoA protein that plays a critical role in controlling actin movement in both mechanisms. However, some of the key components differ between these two transmigration processes. CAV1 genes emerged during Trichoplax adhaerens, and it was only reported in transcellular process. Paracellular process is dependent on PECAM1. PECAM1 emerged from FASL5 during Zebrafish divergence. Lastly, both systems employ late divergent genes such as ICAM1 and VECAM1. Taken together, our results suggest that these two systems constitute two different mechanical sensing mechanisms of immune cell infiltrations of the brain, yet these two systems are connected. We postulate that the mechanical properties of the cellular polarity is the main driving force determining the migration pathway. Our analysis indicates that both systems coevolved with immune cells, evolving to a higher level of complexity in association with the evolution of the immune system.

Influence of Early Descemet Stripping Endothelial Keratoplasty on Visual Outcomes in Pseudophakic Corneal Edema

2016 ◽  
Vol 172 ◽  
pp. 58-63 ◽  
Author(s):  
Sarah B. Weissbart ◽  
Kristin M. Hammersmith ◽  
Brandon D. Ayres ◽  
Christopher J. Rapuano ◽  
Parveen K. Nagra ◽  
...  
Keyword(s):  
Corneal Edema ◽  
Endothelial Keratoplasty ◽  
Descemet Stripping Endothelial Keratoplasty ◽  
Visual Outcomes

scholarly journals Transplantation of iPSC-derived corneal endothelial substitutes in a monkey corneal edema model

2021 ◽  
pp. 102497
Author(s):  
Shin Hatou ◽  
Tomoko Sayano ◽  
Kazunari Higa ◽  
Emi Inagaki ◽  
Yuji Okano ◽  
...  
Keyword(s):  
Corneal Edema ◽  
Edema Model

The Effect of Contact Lens Induced Corneal Edema on Goldmann Applanation Tonometry Measurements

2007 ◽  
Vol 16 (1) ◽  
pp. 153-158 ◽  
Author(s):  
Kirsten E. Hamilton ◽  
David C. Pye ◽  
Amir Hali ◽  
Cassie Lin ◽  
Phillip Kam ◽  
...  
Keyword(s):  
Contact Lens ◽  
Corneal Edema ◽  
Applanation Tonometry ◽  
Goldmann Applanation Tonometry

Amantadine induced reversible corneal edema

2011 ◽  
Vol 18 (2) ◽  
pp. 298-299 ◽  
Author(s):  
Milind Deogaonkar ◽  
Kathy Wilson ◽  
Jerrold Vitek
Keyword(s):  
Corneal Edema
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