scholarly journals Bright-Field Dual-Color Chromogenic In Situ Hybridization for Diagnosing Echinoderm Microtubule-Associated Protein-Like 4-Anaplastic Lymphoma Kinase-Positive Lung Adenocarcinomas

2011 ◽  
Vol 6 (10) ◽  
pp. 1677-1686 ◽  
Author(s):  
Akihiko Yoshida ◽  
Koji Tsuta ◽  
Hiroaki Nitta ◽  
Yutaka Hatanaka ◽  
Hisao Asamura ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Anaplastic Lymphoma Kinase ◽  
Bright Field ◽  
Anaplastic Lymphoma ◽  
Dual Color ◽  
Chromogenic In Situ Hybridization ◽  
Lung Adenocarcinomas

scholarly journals Evaluation of Myc Gene Amplification in Prostate Cancer Using a Dual Color Chromogenic in-Situ Hybridization (Dual CISH) Assay

2013 ◽  
Vol 1 (2) ◽  
pp. 81
Author(s):  
Daniel Lerda ◽  
Marta Cabrera ◽  
Jorge Flores ◽  
Luis Gutierrez ◽  
Armando Chierichetti ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
In Situ Hybridization ◽  
Gene Amplification ◽  
Dual Color ◽  
Myc Gene ◽  
Chromogenic In Situ Hybridization

Evaluation of HER2 gene amplification in invasive breast cancer using a dual-color chromogenic in situ hybridization (dual CISH)

2010 ◽  
Vol 60 (7) ◽  
pp. 510-515 ◽  
Author(s):  
Nobuaki Kato ◽  
Hitoshi Itoh ◽  
Akihiko Serizawa ◽  
Yutaka Hatanaka ◽  
Shinobu Umemura ◽  
...  
Keyword(s):  
Breast Cancer ◽  
In Situ Hybridization ◽  
Gene Amplification ◽  
Invasive Breast Cancer ◽  
Her2 Gene ◽  
Dual Color ◽  
Her2 Gene Amplification ◽  
Chromogenic In Situ Hybridization

Dual-Color Ultrasensitive Bright-Field RNA In Situ Hybridization with RNAscope

2014 ◽  
pp. 139-149 ◽  
Author(s):  
Hongwei Wang ◽  
Nan Su ◽  
Li-Chong Wang ◽  
Xingyong Wu ◽  
Son Bui ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Bright Field ◽  
Dual Color ◽  
Rna In Situ Hybridization

scholarly journals The Use of Three-Dimensional DNA Fluorescent In Situ Hybridization (3D DNA FISH) for the Detection of Anaplastic Lymphoma Kinase (ALK) in Non-Small Cell Lung Cancer (NSCLC) Circulating Tumor Cells

Cells ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 1465 ◽  
Author(s):  
Arutha Kulasinghe ◽  
Yenkai Lim ◽  
Joanna Kapeleris ◽  
Majid Warkiani ◽  
Ken O’Byrne ◽  
...  
Keyword(s):  
Lung Cancer ◽  
In Situ Hybridization ◽  
Tumor Cells ◽  
Anaplastic Lymphoma Kinase ◽  
Fluorescent In Situ Hybridization ◽  
Small Cell ◽  
Small Cell Lung ◽  
Anaplastic Lymphoma ◽  
Nsclc Patients

Tumor tissue biopsy is often limited for non-small cell lung cancer (NSCLC) patients and alternative sources of tumoral information are desirable to determine molecular alterations such as anaplastic lymphoma kinase (ALK) rearrangements. Circulating tumor cells (CTCs) are an appealing component of liquid biopsies, which can be sampled serially over the course of treatment. In this study, we enrolled a cohort of ALK-positive (n = 8) and ALK-negative (n = 12) NSCLC patients, enriched for CTCs using spiral microfluidic technology and performed DNA fluorescent in situ hybridization (FISH) for ALK. CTCs were identified in 12/20 NSCLC patients ranging from 1 to 26 CTCs/7.5 mL blood. Our study revealed that 3D imaging of CTCs for ALK translocations captured a well-defined separation of 3′ and 5′ signals indicative of ALK translocations and overlapping 3′/5′ signal was easily resolved by imaging through the nuclear volume. This study provides proof-of-principle for the use of 3D DNA FISH in the determination of CTC ALK translocations in NSCLC.

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