A comparative analysis of immunohistochemistry and fluorescent in situ hybridization assay to detect anaplastic lymphoma kinase status in lung adenocarcinoma cases: A search for a testing algorithm

2017 ◽  
Vol 54 (1) ◽  
pp. 148 ◽  
Author(s):  
PB Wagle ◽  
NA Jambhekar ◽  
R Kumar ◽  
K Prabhash ◽  
CS Pramesh ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Comparative Analysis ◽  
Lung Adenocarcinoma ◽  
Anaplastic Lymphoma Kinase ◽  
Fluorescent In Situ Hybridization ◽  
Hybridization Assay ◽  
Anaplastic Lymphoma ◽  
Testing Algorithm

scholarly journals The Use of Three-Dimensional DNA Fluorescent In Situ Hybridization (3D DNA FISH) for the Detection of Anaplastic Lymphoma Kinase (ALK) in Non-Small Cell Lung Cancer (NSCLC) Circulating Tumor Cells

Cells ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 1465 ◽  
Author(s):  
Arutha Kulasinghe ◽  
Yenkai Lim ◽  
Joanna Kapeleris ◽  
Majid Warkiani ◽  
Ken O’Byrne ◽  
...  
Keyword(s):  
Lung Cancer ◽  
In Situ Hybridization ◽  
Tumor Cells ◽  
Anaplastic Lymphoma Kinase ◽  
Fluorescent In Situ Hybridization ◽  
Small Cell ◽  
Small Cell Lung ◽  
Anaplastic Lymphoma ◽  
Nsclc Patients

Tumor tissue biopsy is often limited for non-small cell lung cancer (NSCLC) patients and alternative sources of tumoral information are desirable to determine molecular alterations such as anaplastic lymphoma kinase (ALK) rearrangements. Circulating tumor cells (CTCs) are an appealing component of liquid biopsies, which can be sampled serially over the course of treatment. In this study, we enrolled a cohort of ALK-positive (n = 8) and ALK-negative (n = 12) NSCLC patients, enriched for CTCs using spiral microfluidic technology and performed DNA fluorescent in situ hybridization (FISH) for ALK. CTCs were identified in 12/20 NSCLC patients ranging from 1 to 26 CTCs/7.5 mL blood. Our study revealed that 3D imaging of CTCs for ALK translocations captured a well-defined separation of 3′ and 5′ signals indicative of ALK translocations and overlapping 3′/5′ signal was easily resolved by imaging through the nuclear volume. This study provides proof-of-principle for the use of 3D DNA FISH in the determination of CTC ALK translocations in NSCLC.

Clinical Evaluation of a Multi-target Fluorescent in Situ Hybridization Assay for Detection of Bladder Cancer

2002 ◽  
Vol 168 (5) ◽  
pp. 1950-1954 ◽  
Author(s):  
MICHAEL F. SAROSDY ◽  
PAUL SCHELLHAMMER ◽  
GARY BOKINSKY ◽  
PAUL KAHN ◽  
ROBERTO CHAO ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
In Situ Hybridization ◽  
Clinical Evaluation ◽  
Fluorescent In Situ Hybridization ◽  
Hybridization Assay

scholarly journals Direct Visualization of Propionibacterium acnes in Prostate Tissue by Multicolor Fluorescent In Situ Hybridization Assay

2007 ◽  
Vol 45 (11) ◽  
pp. 3721-3728 ◽  
Author(s):  
O. A. Alexeyev ◽  
I. Marklund ◽  
B. Shannon ◽  
I. Golovleva ◽  
J. Olsson ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescent In Situ Hybridization ◽  
Propionibacterium Acnes ◽  
Hybridization Assay ◽  
Prostate Tissue ◽  
Direct Visualization
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