Superficial Angiomyxomas Frequently Demonstrate Loss of Protein Kinase A Regulatory Subunit 1 Alpha Expression

Glioblastoma is the most malignant and most common form of brain tumor, still today associated with a poor 14-months median survival from diagnosis. Protein kinase A, particularly its regulatory subunit R2Alpha, presents a typical intracellular distribution in glioblastoma cells compared to the healthy brain parenchyma and this peculiarity might be exploited in a therapeutic setting. In the present study, a third-generation lentiviral system for delivery of shRNA targeting the regulatory subunit R2Alpha of protein kinase A was developed. Generated lentiviral vectors are able to induce an efficient and stable downregulation of R2Alpha in different cellular models, including non-stem and stem-like glioblastoma cells. In addition, our data suggest a potential correlation between silencing of the regulatory subunit of protein kinase A and reduced viability of tumor cells, apparently due to a reduction in replication rate. Thus, our findings support the role of protein kinase A as a promising target for novel anti-glioma therapies.

Download Full-text

The crystal structure of yeast regulatory subunit reveals key evolutionary insights into Protein Kinase A oligomerization

Journal of Structural Biology ◽

10.1016/j.jsb.2021.107732 ◽

2021 ◽

pp. 107732

Author(s):

Nicolás González Bardeci ◽

Enzo Tofolón ◽

Felipe Trajtenberg ◽

Julio Caramelo ◽

Nicole Larrieux ◽

...

Keyword(s):

Crystal Structure ◽

Protein Kinase ◽

Protein Kinase A ◽

Regulatory Subunit ◽

Kinase A

Download Full-text

Overexpression of the regulatory subunit of protein kinase A increases heterologous protein expression in Pichia pastoris

Biotechnology Letters ◽

10.1007/s10529-020-02977-z ◽

2020 ◽

Vol 42 (12) ◽

pp. 2685-2692 ◽

Cited By ~ 1

Author(s):

Xihao Liao ◽

Wenyang Lin ◽

Nanzhu Chen ◽

Lu Li ◽

Dafu Huang ◽

...

Keyword(s):

Protein Kinase ◽

Pichia Pastoris ◽

Protein Expression ◽

Protein Kinase A ◽

Heterologous Protein ◽

Regulatory Subunit ◽

Heterologous Protein Expression ◽

Kinase A

Download Full-text

The Role of Uncoupling Protein 1 in the Metabolism and Adiposity of RIIβ-Protein Kinase A-Deficient Mice

Molecular Endocrinology ◽

10.1210/me.2004-0194 ◽

2004 ◽

Vol 18 (9) ◽

pp. 2302-2311 ◽

Cited By ~ 21

Author(s):

Michael A. Nolan ◽

Maria A. Sikorski ◽

G. Stanley McKnight

Keyword(s):

Adipose Tissue ◽

Oxygen Consumption ◽

Protein Kinase ◽

Protein Kinase A ◽

Uncoupling Protein ◽

Mrna Level ◽

Regulatory Subunit ◽

Uncoupling Protein 1 ◽

Brown Adipose ◽

Kinase A

Abstract Mice lacking the RIIβ regulatory subunit of protein kinase A exhibit a 50% reduction in white adipose tissue stores compared with wild-type littermates and are resistant to diet-induced obesity. RIIβ−/− mice also have an increase in resting oxygen consumption along with a 4-fold increase in the brown adipose-specific mitochondrial uncoupling protein 1 (UCP1). In this study, we examined the basis for UCP1 induction and tested the hypothesis that the induced levels of UCP1 in RIIβ null mice are essential for the lean phenotype. The induction of UCP1 occurred at the protein but not the mRNA level and correlated with an increase in mitochondria in brown adipose tissue. Mice lacking both RIIβ and UCP1 (RIIβ−/−/Ucp1−/−) were created, and the key parameters of metabolism and body composition were studied. We discovered that RIIβ−/− mice exhibit nocturnal hyperactivity in addition to the increased oxygen consumption at rest. Disruption of UCP1 in RIIβ−/− mice reduced basal oxygen consumption but did not prevent the nocturnal hyperactivity. The double knockout animals also retained the lean phenotype of the RIIβ null mice, demonstrating that induction of UCP1 and increased resting oxygen consumption is not the cause of leanness in the RIIβ mutant mice.

Download Full-text

Complex effects of kinase localization revealed by compartment-specific regulation of protein kinase A activity

10.1101/2021.04.08.439038 ◽

2021 ◽

Author(s):

Rebecca LaCroix ◽

Benjamin Lin ◽

Andre Levchenko

Keyword(s):

Cell Migration ◽

Protein Kinase ◽

Protein Kinase A ◽

Kinase Activity ◽

Single Cells ◽

Regulatory Subunit ◽

Dependent Manner ◽

Concentration Dependent Manner ◽

Specific Regulation ◽

Kinase A

SummaryKinase activity in signaling networks frequently depends on regulatory subunits that can both inhibit activity by interacting with the catalytic subunits and target the kinase to distinct molecular partners and subcellular compartments. Here, using a new synthetic molecular interaction system, we show that translocation of a regulatory subunit of the protein kinase A (PKA-R) to the plasma membrane has a paradoxical effect on the membrane kinase activity. It can both enhance it at lower translocation levels, even in the absence of signaling inputs, and inhibit it at higher translocation levels, suggesting its role as a linker that can both couple and decouple signaling processes in a concentration-dependent manner. We further demonstrate that superposition of gradients of PKA-R abundance across single cells can control the directionality of cell migration, reversing it at high enough input levels. Thus complex in vivo patterns of PKA-R localization can drive complex phenotypes, including cell migration.

Download Full-text