scholarly journals Impact of Red Blood Cells on Function and Metabolism of Porcine Deceased Donor Kidneys During Normothermic Machine Perfusion

2021 ◽  
Vol Publish Ahead of Print ◽  
Author(s):  
Leonie H. Venema ◽  
L. Leonie van Leeuwen ◽  
Rene A. Posma ◽  
Harry van Goor ◽  
Rutger J. Ploeg ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Deceased Donor ◽  
Machine Perfusion ◽  
Normothermic Machine Perfusion

scholarly journals Perfusion settings and additives in liver normothermic machine perfusion with red blood cells as oxygen carrier. A systematic review of human and porcine perfusion protocols

2018 ◽  
Vol 31 (9) ◽  
pp. 956-969 ◽  
Author(s):  
Dilmurodjon Eshmuminov ◽  
Filippo Leoni ◽  
Marcel André Schneider ◽  
Dustin Becker ◽  
Xavier Muller ◽  
...  
Keyword(s):  
Systematic Review ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Oxygen Carrier ◽  
Machine Perfusion ◽  
Normothermic Machine Perfusion

scholarly journals Reply to “Ex situ normothermic machine perfusion of donor livers using a haemoglobin-based oxygen carrier: a viable alternative to red blood cells”

2018 ◽  
Vol 31 (11) ◽  
pp. 1283-1284 ◽  
Author(s):  
Dilmurodjon Eshmuminov ◽  
Filippo Leoni ◽  
Marcel André Schneider ◽  
Dustin Becker ◽  
Xavier Muller ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Oxygen Carrier ◽  
Viable Alternative ◽  
Ex Situ ◽  
Machine Perfusion ◽  
Normothermic Machine Perfusion

scholarly journals Ex situ normothermic machine perfusion of donor livers using a haemoglobin-based oxygen carrier: a viable alternative to red blood cells

2018 ◽  
Vol 31 (11) ◽  
pp. 1281-1282 ◽  
Author(s):  
Yvonne de Vries ◽  
Otto B. van Leeuwen ◽  
Alix P. M. Matton ◽  
Masato Fujiyoshi ◽  
Vincent E. de Meijer ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Oxygen Carrier ◽  
Viable Alternative ◽  
Ex Situ ◽  
Machine Perfusion ◽  
Normothermic Machine Perfusion

scholarly journals The effect of blood cells retained in rat livers during static cold storage on viability outcomes during normothermic machine perfusion

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Omar Haque ◽  
Casie A. Pendexter ◽  
Benjamin T. Wilks ◽  
Ehab O. A. Hafiz ◽  
James F. Markmann ◽  
...  
Keyword(s):  
Cold Storage ◽  
Blood Cells ◽  
Energy Charge ◽  
Ischemic Injury ◽  
Machine Perfusion ◽  
University Of Wisconsin ◽  
Normothermic Machine Perfusion ◽  
Preservation Solutions ◽  
Positive Correlations ◽  
Rat Livers

AbstractIn transplantation, livers are transported to recipients using static cold storage (SCS), whereby livers are exposed to cold ischemic injury that contribute to post-transplant risk factors. We hypothesized that flushing organs during procurement with cold preservation solutions could influence the number of donor blood cells retained in the allograft thereby exacerbating cold ischemic injury. We present the results of rat livers that underwent 24 h SCS after being flushed with a cold University of Wisconsin (UW) solution versus room temperature (RT) lactated ringers (LR) solution. These results were compared to livers that were not flushed prior to SCS and thoroughly flushed livers without SCS. We used viability and injury metrics collected during normothermic machine perfusion (NMP) and the number of retained peripheral cells (RPCs) measured by histology to compare outcomes. Compared to the cold UW flush group, livers flushed with RT LR had lower resistance, lactate, AST, and ALT at 6 h of NMP. The number of RPCs also had significant positive correlations with resistance, lactate, and potassium levels and a negative correlation with energy charge. In conclusion, livers exposed to cold UW flush prior to SCS appear to perform worse during NMP, compared to RT LR flush.

Visualization of Endothelial Cell Structure During Machine Perfusion Preservation of Livers

2002 ◽  
Author(s):  
Shailendra Jain ◽  
Saurin Purohit ◽  
Jian Zhang ◽  
Mark G. Clemens ◽  
Charles Y. Lee
Keyword(s):  
Confocal Microscopy ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Cell Structure ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Machine Perfusion ◽  
Success Rates ◽  
University Of Wisconsin ◽  
Heart Beating

The development of machine perfusion preservation (MPP) of kidney has led to significant improvements and greater success rates in kidney transplantation by providing superior preserved tissue and viable non-heart-beating donor tissue. However, machine perfusion of livers has not been successful in improving preservation. Currently, the major cause of damage associated with MPP of livers remains unclear. Previous studies showed increased vascular resistance and blockages during and after 24hrs MPP but no direct evidence existed. Utilizing a novel two colors fluorophores labeling, an intravital microscopic study was conducted to obtain real time images and confocal microscopy to get detailed images in order to correlate fluorescent-tagged endothelial cells (ECs) with red cell stasis. Fluorescein isothiocynate (FITC) was used to label red blood cells (RBCs) and DiI acetylated low-density lipoprotein (DiI acLDL) was used to mark ECs. Structure of ECs was recorded and assessed during 24hrs MPP with University of Wisconsin (UW) solution at 4°C with a flow rate of 4ml/min. Images recorded from intravital microscopy and confocal microscopy show ECs rounding over a period of 24 hrs and subsequent red blood cells stasis after 24hrs MPP and during rewarming.

scholarly journals Oxygen Transport during Ex Situ Machine Perfusion of Donor Livers Using Red Blood Cells or Artificial Oxygen Carriers

2020 ◽  
Vol 22 (1) ◽  
pp. 235
Author(s):  
Silke B. Bodewes ◽  
Otto B. van Leeuwen ◽  
Adam M. Thorne ◽  
Bianca Lascaris ◽  
Rinse Ubbink ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Oxygen Transport ◽  
Oxygen Delivery ◽  
Blood Cells ◽  
Marine Invertebrate ◽  
Oxygen Carrier ◽  
Ex Situ ◽  
Machine Perfusion ◽  
Oxygen Carriers ◽  
Advantages And Disadvantages

Oxygenated ex situ machine perfusion of donor livers is an alternative for static cold preservation that can be performed at temperatures from 0 °C to 37 °C. Organ metabolism depends on oxygen to produce adenosine triphosphate and temperatures below 37 °C reduce the metabolic rate and oxygen requirements. The transport and delivery of oxygen in machine perfusion are key determinants in preserving organ viability and cellular function. Oxygen delivery is more challenging than carbon dioxide removal, and oxygenation of the perfusion fluid is temperature dependent. The maximal oxygen content of water-based solutions is inversely related to the temperature, while cellular oxygen demand correlates positively with temperature. Machine perfusion above 20 °C will therefore require an oxygen carrier to enable sufficient oxygen delivery to the liver. Human red blood cells are the most physiological oxygen carriers. Alternative artificial oxygen transporters are hemoglobin-based oxygen carriers, perfluorocarbons, and an extracellular oxygen carrier derived from a marine invertebrate. We describe the principles of oxygen transport, delivery, and consumption in machine perfusion for donor livers using different oxygen carrier-based perfusion solutions and we discuss the properties, advantages, and disadvantages of these carriers and their use.

scholarly journals The effect of blood cells retained in rat livers during static cold storage on viability outcomes during normothermic machine perfusion

2021 ◽  
Author(s):  
Omar Haque ◽  
Casie A. Pendexter ◽  
Benjamin T. Wilks ◽  
Ehab O. A. Hafiz ◽  
James F. Markmann ◽  
...  
Keyword(s):  
Cold Storage ◽  
Blood Cells ◽  
Energy Charge ◽  
Ischemic Injury ◽  
Machine Perfusion ◽  
University Of Wisconsin ◽  
Normothermic Machine Perfusion ◽  
Preservation Solutions ◽  
Positive Correlations ◽  
Rat Livers

Abstract In transplantation, livers are transported to recipients using static cold storage (SCS), whereby livers are exposed to cold ischemic injury that contribute to post-transplant risk factors. We hypothesized that flushing organs during procurement with cold preservation solutions could influence the number of donor blood cells retained in the allograft thereby exacerbating cold ischemic injury. We present the results of rat livers that underwent 24h SCS after being flushed with a cold University of Wisconsin (UW) solution versus room temperature (RT) lactated ringers (LR) solution. These results were compared to livers that were not flushed prior to SCS and thoroughly flushed livers without SCS. We used viability and injury metrics collected during normothermic machine perfusion (NMP) and the number of retained peripheral cells (RPCs) measured by histology to compare outcomes. Compared to the cold UW flush group, livers flushed with RT LR had lower resistance, lactate, AST, and ALT at 6 hours of NMP. The number of RPCs also had significant positive correlations with resistance, lactate, and potassium levels and a negative correlation with energy charge. In conclusion, livers exposed to cold UW flush prior to SCS appear to perform worse during NMP, compared to RT LR flush.

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