scholarly journals Liposomes in tissue engineering and regenerative medicine

2014 ◽  
Vol 11 (101) ◽  
pp. 20140459 ◽  
Author(s):  
Nelson Monteiro ◽  
Albino Martins ◽  
Rui L. Reis ◽  
Nuno M. Neves
Keyword(s):  
Tissue Engineering ◽  
Regenerative Medicine ◽  
Potential Role ◽  
Lipid Membrane ◽  
Cultured Cells ◽  
Cell Types ◽  
Biomaterial Scaffolds ◽  
Bioactive Agents ◽  
The 1960S

Liposomes are vesicular structures made of lipids that are formed in aqueous solutions. Structurally, they resemble the lipid membrane of living cells. Therefore, they have been widely investigated, since the 1960s, as models to study the cell membrane, and as carriers for protection and/or delivery of bioactive agents. They have been used in different areas of research including vaccines, imaging, applications in cosmetics and tissue engineering. Tissue engineering is defined as a strategy for promoting the regeneration of tissues for the human body. This strategy may involve the coordinated application of defined cell types with structured biomaterial scaffolds to produce living structures. To create a new tissue, based on this strategy, a controlled stimulation of cultured cells is needed, through a systematic combination of bioactive agents and mechanical signals. In this review, we highlight the potential role of liposomes as a platform for the sustained and local delivery of bioactive agents for tissue engineering and regenerative medicine approaches.

The potential role of telocytes in Tissue Engineering and Regenerative Medicine

2016 ◽  
Vol 55 ◽  
pp. 70-78 ◽  
Author(s):  
Anja M. Boos ◽  
Annika Weigand ◽  
Rebekka Brodbeck ◽  
Justus P. Beier ◽  
Andreas Arkudas ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Regenerative Medicine ◽  
Potential Role

The Potential Role of Telocytes for Tissue Engineering and Regenerative Medicine

2016 ◽  
pp. 139-147 ◽  
Author(s):  
Raymund E. Horch ◽  
Annika Weigand ◽  
Justus P. Beier ◽  
Andreas Arkudas ◽  
Anja M. Boos
Keyword(s):  
Tissue Engineering ◽  
Regenerative Medicine ◽  
Potential Role

The Potential of Mesothelial Cells in Tissue Engineering and Regenerative Medicine Applications

2007 ◽  
Vol 30 (6) ◽  
pp. 527-540 ◽  
Author(s):  
S.E. Herrick ◽  
S.E. Mutsaers
Keyword(s):  
Tissue Engineering ◽  
Regenerative Medicine ◽  
Progenitor Cells ◽  
Adhesion Formation ◽  
Cell Types ◽  
Mesothelial Cells ◽  
Formation Mechanisms ◽  
And Migration ◽  
Proliferation And Migration

Injury to the serosa through injurious agents such as radiation, surgery, infection and disease results in the loss of the protective surface mesothelium and often leads to fibrous adhesion formation. Mechanisms that increase the rate of mesothialisation are therefore actively being investigated in order to reduce the formation of adhesions. These include intraperitoneal delivery of cultured mesothelial cells as well as administration of factors that are known to increase mesothelial proliferation and migration. An exciting alternative that has only recently received attention, is the possible role of mesothelial progenitor cells in the repair and regeneration of denuded serosal areas. Accumulating evidence suggests that such a population exists and under certain conditions is able to form a number of defined cell types indicating a degree of plasticity. Such properties may explain the extensive use of mesothelial cells in various tissue engineering applications including the development of vascular conduits and peripheral nerve replacements. It is likely that with the rapid explosion in the fields of tissue engineering and regenerative medicine, a greater understanding of the potential of mesothelial progenitor cells to repair, replace and possibly regenerate damaged or defective tissue will be uncovered.

scholarly journals Evolution of Diploid Progenitor Lung Cell Applications: From Optimized Biotechnological Substrates to Potential Active Pharmaceutical Ingredients in Respiratory Tract Regenerative Medicine

Cells ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2526
Author(s):  
Alexis Laurent ◽  
Philippe Abdel-Sayed ◽  
Nathalie Hirt-Burri ◽  
Corinne Scaletta ◽  
Murielle Michetti ◽  
...  
Keyword(s):  
Regenerative Medicine ◽  
Respiratory Tract ◽  
Lung Tissue ◽  
Cell Types ◽  
Active Pharmaceutical Ingredients ◽  
Vaccine Production ◽  
Diploid Progenitor ◽  
Pharmaceutical Ingredients ◽  
The 1960S ◽  
Pharmacopoeial Requirements

The objective of this review is to describe the evolution of lung tissue-derived diploid progenitor cell applications, ranging from historical biotechnological substrate functions for vaccine production and testing to current investigations around potential therapeutic use in respiratory tract regenerative medicine. Such cell types (e.g., MRC-5 or WI-38 sources) were extensively studied since the 1960s and have been continuously used over five decades as safe and sustainable industrial vaccine substrates. Recent research and development efforts around diploid progenitor lung cells (e.g., FE002-Lu or Walvax-2 sources) consist in qualification for potential use as optimal and renewed vaccine production substrates and, alternatively, for potential therapeutic applications in respiratory tract regenerative medicine. Potentially effective, safe, and sustainable cell therapy approaches for the management of inflammatory lung diseases or affections and related symptoms (e.g., COVID-19 patients and burn patient severe inhalation syndrome) using local homologous allogeneic cell-based or cell-derived product administrations are considered. Overall, lung tissue-derived progenitor cells isolated and produced under good manufacturing practices (GMP) may be used with high versatility. They can either act as key industrial platforms optimally conforming to specific pharmacopoeial requirements or as active pharmaceutical ingredients (API) for potentially effective promotion of lung tissue repair or regeneration.

scholarly journals Rethinking Development Strategy –The Importance of the Rural Non Farm Economy in Growth and Poverty Reduction in Pakistan

2008 ◽  
Vol 13 (Special Edition) ◽  
pp. 189-204 ◽  
Author(s):  
Sohail Jehangir Malik
Keyword(s):  
Development Strategy ◽  
Poverty Reduction ◽  
Potential Role ◽  
Development Theory ◽  
Investment Climate ◽  
Agricultural Growth ◽  
Services Sector ◽  
Agriculture Sector ◽  
The 1960S

The structural transformation of Pakistan’s economy has not been accompanied by a concomitant decline in the proportion of labor employed in agriculture. While this transformation has resulted in a non-farm sector that is large and growing it has not lead to the rapid absorption of the pool of relatively low productivity labor away from the agriculture sector, as predicted by conventional development theory embodied in the models of the 1960s. Despite the obvious importance of the role of a vibrant rural non-farm economy (RNFE), and in particular, a vibrant non-farm services sector to address the challenges of poverty, food security, agricultural growth and rural development, this sector has received inadequate attention in the debate in Pakistan. Based on a review of literature and data from two large surveys – the Rural Investment Climate Survey of Pakistan 2005 and the Surveys of Domestic Commerce 2007 – this paper attempts to analyze the factors underlying the low level of development of the rural non farm economy and the potential role it can play in Pakistan’s economic development.

scholarly journals Detachment of cultured cells from the substratum induced by the neutrophil-derived oxidant NH2Cl: synergistic role of phosphotyrosine and intracellular Ca2+ concentration.

1995 ◽  
Vol 131 (2) ◽  
pp. 509-524 ◽  
Author(s):  
T Y Nakamura ◽  
I Yamamoto ◽  
H Nishitani ◽  
T Matozaki ◽  
T Suzuki ◽  
...  
Keyword(s):  
Cardiac Myocytes ◽  
Cultured Cells ◽  
Tyrosine Phosphatase ◽  
Cell Types ◽  
Human Neutrophils ◽  
Atpase Inhibitor ◽  
Culture Dish ◽  
Protein Tyrosine Phosphatase Inhibitor ◽  
Peeling Off

The neutrophil-derived, membrane-permeating oxidant, NH2Cl, (but not the non-membrane-permeating chloramine, taurine-NHCl) induced detachment of fetal mouse cardiac myocytes and other cell types (fibroblasts, epithelial cells, and endothelial cells) from the culture dish, concomitant with cell shrinkage ("peeling off"). Stimulated human neutrophils also induced peeling off of cultured mouse cardiac myocytes when the latter were pretreated with inhibitors of .OH and elastase. Immunofluorescence microscopy revealed that the NH2Cl-induced peeling off of WI-38 fibroblasts is accompanied by disorganization of integrin alpha 5 beta 1, vinculin, stress fibers, and phosphotyrosine (p-Tyr)-containing proteins. Decrease in the content of the p-Tyr-containing proteins of the NH2Cl-treated cells was analyzed by immunoblotting techniques. Coating of fibronectin on the culture dish prevented both NH2Cl-induced peeling off and a decrease in p-Tyr content. Preincubation with a protein-tyrosine phosphatase inhibitor, sodium orthovanadate (Na3VO4), also prevented NH2Cl-induced peeling off, suggesting that dephosphorylation of p-Tyr is necessary for peeling off. NH2Cl-induced peeling off was accompanied by an increase in intracellular Ca2+ concentration ([Ca2+]i) in mouse cardiac myocytes and WI-38 fibroblasts. The absence of extracellular Ca2+ prevented both NH2Cl-induced peeling off and increased [Ca2+]i, both of which did occur on subsequent incubation of the cells in Ca2+-containing medium. These observations suggest that an increase in [Ca2+]i is also necessary for peeling off. Depletion of microsomal and cytosolic Ca2+ by incubation with the microsomal Ca2+-ATPase inhibitor 2',5'-di(tert-butyl)-1,4-benzohydroquinone (BHQ) plus EGTA prevented both NH2Cl-induced increases in [Ca2+]i and peeling off. Direct inhibition of microsomal Ca2+ pump activity by NH2Cl may participate in the NH2Cl-induced [Ca2+]i increment. A combination of p-Tyr dephosphorylation by genistein (an inhibitor of tyrosine kinase) and an increase in [Ca2+]i by BHQ could also induce peeling off. All these observations suggest a synergism between p-Tyr dephosphorylation and increased [Ca2+]i in NH2Cl-induced peeling off.

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