Tissue Engineering, Regenerative Medicine, and Rejuvenation in 2010: The Role of Adipose-Derived Stem Cells

AbstractTissue engineering and regenerative medicine has become the treatment of choice for several degenerative diseases. It involves the repairing or replacing of diseased or damaged cells or tissues. Stem cells have a key role to play in this multidisciplinary science because of their capacity to differentiate into several lineages. Adipose derived stem cells (ADSCs) are adult mesenchymal stem cells that are easily harvested and have the capacity to differentiate into cartilage, bone, smooth muscle, fat, liver and nerve cells. ADSCs have been found to differentiate into smooth muscle cells which play major roles in diseases such as asthma, hypertension, cancer and arteriosclerosis. Low Intensity Laser Irradiation (LILI), which involves the application of monochromatic light, has been found to increase viability, proliferation and differentiation in several types of cells including ADSCs. This review discusses the role of ADSCs, smooth muscle cells and LILI in the science of tissue engineering and regenerative medicine.

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Fat on sale: role of adipose-derived stem cells as anti-fibrosis agent in regenerative medicine

Stem Cell Research & Therapy ◽

10.1186/s13287-015-0246-3 ◽

2015 ◽

Vol 6 (1) ◽

Cited By ~ 8

Author(s):

Manoj K. Gupta ◽

Amrendra Kumar Ajay

Keyword(s):

Stem Cells ◽

Regenerative Medicine ◽

Adipose Derived Stem Cells

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Adipose Derived Stem Cells: Current State of the Art and Prospective Role in Regenerative Medicine and Tissue Engineering

Regenerative Medicine and Tissue Engineering ◽

10.5772/55924 ◽

2013 ◽

Author(s):

Vincenzo Vindigni ◽

Giorgio Giatsidis ◽

Francesco Reho ◽

Erica Dalla Venezia ◽

Marco Mammana ◽

...

Keyword(s):

Tissue Engineering ◽

Stem Cells ◽

Regenerative Medicine ◽

State Of The Art ◽

Adipose Derived Stem Cells ◽

Current State

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Stem Cells in Equine Veterinary Practice—Current Trends, Risks, and Perspectives

Journal of Clinical Medicine ◽

10.3390/jcm8050675 ◽

2019 ◽

Vol 8 (5) ◽

pp. 675

Author(s):

Katarzyna Kornicka ◽

Florian Geburek ◽

Michael Röcken ◽

Krzysztof Marycz

Keyword(s):

Tissue Engineering ◽

Stem Cells ◽

Regenerative Medicine ◽

Clinical Application ◽

Scientific Community ◽

Adipose Derived Stem Cells ◽

Special Issue ◽

Veterinary Practice ◽

Current Trends

With this Editorial, we introduce the Special Issue “Adipose-Derived Stem Cells and Their Extracellular Microvesicles (ExMVs) for Tissue Engineering and Regenerative Medicine Applications” to the scientific community. In this issue, we focus on regenerative medicine, stem cells, and their clinical application.

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Generation of an azide-modified extracellular matrix by adipose-derived stem cells using metabolic glycoengineering

Current Directions in Biomedical Engineering ◽

10.1515/cdbme-2019-0099 ◽

2019 ◽

Vol 5 (1) ◽

pp. 393-395 ◽

Cited By ~ 3

Author(s):

Svenja Nellinger ◽

Silke Keller ◽

Alexander Southan ◽

Valentin Wittmann ◽

Ann-Cathrin Volz ◽

...

Keyword(s):

Tissue Engineering ◽

Stem Cells ◽

Extracellular Matrix ◽

Regenerative Medicine ◽

Functional Groups ◽

Click Reaction ◽

Fluorescent Dye ◽

Adipose Derived Stem Cells ◽

Metabolic Glycoengineering

AbstractNatural extracellular matrix (ECM) represents an ideal biomaterial for tissue engineering and regenerative medicine approaches. For further functionalization, there is a need for specific addressable functional groups within this biomaterial. Metabolic glycoengineering (MGE) provides a technique to incorporate modified monosaccharide derivatives into the ECM during their assembly, which was shown by us earlier for the production of a modified fibroblast-derived dermal ECM. In this study, adipose-derived stem cells (ASCs) were treated with the azide-modified monosaccharide derivate 1,3,4,6-tetra-O-acetyl-N-azidoacetylgalactosamine (Ac4GalNAz). Toxicity and viability assays after 24 h and 72 h incubation revealed high biocompatibility of Ac4GalNAz in contact with ASCs. The successful incorporation of the functional azide groups into the glycocalyx and the ECM of the ASCs was proven by conjugation with a fluorescent dye via a copper-catalyzed click reaction. Thus, Ac4GalNAz in combination with ASCs was confirmed to achieve an azidemodified ECM as a multifunctional biomaterial for further applications.

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Adipose-Derived Stem Cells for Tissue Engineering and Regenerative Medicine Applications

Stem Cells International ◽

10.1155/2016/6737345 ◽

2016 ◽

Vol 2016 ◽

pp. 1-19 ◽

Cited By ~ 80

Author(s):

Ru Dai ◽

Zongjie Wang ◽

Roya Samanipour ◽

Kyo-in Koo ◽

Keekyoung Kim

Keyword(s):

Tissue Engineering ◽

Stem Cells ◽

Regenerative Medicine ◽

Three Dimensional ◽

Adipose Derived Stem Cells ◽

Tissue Engineering Scaffolds ◽

Stem Cell Source ◽

Proliferation And Differentiation ◽

Mesenchymal Stem Cell Source

Adipose-derived stem cells (ASCs) are a mesenchymal stem cell source with properties of self-renewal and multipotential differentiation. Compared to bone marrow-derived stem cells (BMSCs), ASCs can be derived from more sources and are harvested more easily. Three-dimensional (3D) tissue engineering scaffolds are better able to mimic thein vivocellular microenvironment, which benefits the localization, attachment, proliferation, and differentiation of ASCs. Therefore, tissue-engineered ASCs are recognized as an attractive substitute for tissue and organ transplantation. In this paper, we review the characteristics of ASCs, as well as the biomaterials and tissue engineering methods used to proliferate and differentiate ASCs in a 3D environment. Clinical applications of tissue-engineered ASCs are also discussed to reveal the potential and feasibility of using tissue-engineered ASCs in regenerative medicine.

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In vitro Three Dimensional Scaffold-free Construct of Human Adipose-derived Stem Cells in Coculture with Endothelial Cells and Fibroblasts

Revista de Chimie ◽

10.37358/rc.17.6.5670 ◽

2017 ◽

Vol 68 (6) ◽

pp. 1341-1344

Author(s):

Grigore Berea ◽

Gheorghe Gh. Balan ◽

Vasile Sandru ◽

Paul Dan Sirbu

Keyword(s):

Tissue Engineering ◽

Stem Cells ◽

Endothelial Cells ◽

Single Cell ◽

Cell Line ◽

Bone Repair ◽

Umbilical Vein ◽

Human Fibroblasts ◽

Three Dimensional ◽

Adipose Derived Stem Cells

Complex interactions between stem cells, vascular cells and fibroblasts represent the substrate of building microenvironment-embedded 3D structures that can be grafted or added to bone substitute scaffolds in tissue engineering or clinical bone repair. Human Adipose-derived Stem Cells (hASCs), human umbilical vein endothelial cells (HUVECs) and normal dermal human fibroblasts (NDHF) can be mixed together in three dimensional scaffold free constructs and their behaviour will emphasize their potential use as seeding points in bone tissue engineering. Various combinations of the aforementioned cell lines were compared to single cell line culture in terms of size, viability and cell proliferation. At 5 weeks, viability dropped for single cell line spheroids while addition of NDHF to hASC maintained the viability at the same level at 5 weeks Fibroblasts addition to the 3D construct of stem cells and endothelial cells improves viability and reduces proliferation as a marker of cell differentiation toward osteogenic line.

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