scholarly journals Denitrification performance of Pseudomonas fluorescens Z03 immobilized by graphene oxide-modified polyvinyl-alcohol and sodium alginate gel beads at low temperature

2020 ◽  
Vol 7 (3) ◽  
pp. 191542 ◽  
Author(s):  
Meizhen Tang ◽  
Jie Jiang ◽  
Qilin Lv ◽  
Bin Yang ◽  
Mingna Zheng ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Low Temperature ◽  
Polyvinyl Alcohol ◽  
Pseudomonas Fluorescens ◽  
Sodium Alginate ◽  
Removal Efficiency ◽  
Nitrogen Removal ◽  
Extracellular Polymeric Substances ◽  
Aerobic Denitrification ◽  
Gel Beads

Improving the effect of microbial denitrification under low-temperature conditions has been a popular focus of research in recent years. In this study, graphene oxide (GO)-modified polyvinyl-alcohol (PVA) and sodium alginate (SA) (GO/PVA–SA) gel beads were used as a heterotrophic nitrification–aerobic denitrification (HN–AD) bacteria ( Pseudomonas fluorescens Z03) carrier to enhance nitrogen removal efficiency levels at low temperatures (6–8°C). The removal efficiency of N H 4     + -N and N O 3       − -N and the variations in concentrations of extracellular polymeric substances (EPS) under different GO doses (0.03–0.15 g l −1 ) were studied. The results indicated that the addition of GO can improve the efficiency of nitrogen removal, and the highest removal efficiency level and highest carbohydrate, protein, and total EPS content levels (50.28 mg, 132.78 mg and 183.06 mg (g GO/PVA–SA gel) −1 , respectively) were obtained with 0.15 g l −1 GO. The simplified Monod model accurately predicted the nitrogen removal efficiency level. These findings suggested that the application of GO serves as an effective means to enhance nitrogen removal by stimulating the activity of HN–AD bacteria.

Anaerobic ammonium oxidation in polyvinyl alcohol and sodium alginate immobilized biomass system: a potential tool to maintain anammox biomass in application

2013 ◽  
Vol 69 (4) ◽  
pp. 718-726 ◽  
Author(s):  
Gang-Li Zhu ◽  
Jia Yan ◽  
Yong-You Hu
Keyword(s):  
Polyvinyl Alcohol ◽  
Sodium Alginate ◽  
Nitrogen Removal ◽  
Enrichment Culture ◽  
Batch Reactor ◽  
Anaerobic Ammonium Oxidation ◽  
Anammox Bacteria ◽  
Ammonium Oxidation ◽  
Gel Beads ◽  
Key Factor

Anaerobic ammonium oxidation (anammox) has been proved to be a promising nitrogen removal method for treating ammonium-rich wastewater. However, because of the low-growth rate of anammox bacteria, maintenance of a sufficient amount of anammox biomass in reactor became a key factor in application. Gel immobilization is an efficient method to prevent biomass from being washed out and to promote hyper-concentrated cultures. This study focused on a nitrogen removal process by anammox enrichment culture immobilized in polyvinyl alcohol and sodium alginate (PVA-SA) gel beads. The rapid startup of reactor demonstrated that gel entrapment was supposed to be a highly effective technique for immobilizing anammox bacteria. The anammox bacteria present in the enrichment were identified to be Jettenia-like species (>98%). Moreover, the effect of hydraulic retention time (HRT), pH, and temperature on immobilized anammox processes were investigated. The effect of pH and temperature on the anammox process was evidently weakened in PVA-SA immobilized gel beads, however, the effect of HRT on the anammox reaction was enhanced. Therefore, a stable operated reactor could be obtained in an anaerobic sequencing batch reactor, which proved gel immobilization was an excellent method to maintain the biomass in anammox reactor for application.

scholarly journals Effect of biomass immobilization and reduced graphene oxide on the microbial community changes and nitrogen removal at low temperatures

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Anna Banach-Wiśniewska ◽  
Mariusz Tomaszewski ◽  
Mohamed S. Hellal ◽  
Aleksandra Ziembińska-Buczyńska
Keyword(s):  
Graphene Oxide ◽  
Microbial Community ◽  
Reduced Graphene Oxide ◽  
Sodium Alginate ◽  
Nitrogen Removal ◽  
Process Conditions ◽  
Ammonium Oxidation ◽  
Gel Beads ◽  
Reduced Graphene ◽  
Community Changes

AbstractThe slow growth rate and high optimal temperatures for the anaerobic ammonium oxidation (anammox) bacteria are significant limitations of the anammox processes application in the treatment of mainstream of wastewater entering wastewater treatment plant (WWTP). In this study, we investigate the nitrogen removal and microbial community changes in sodium alginate (SA) and sodium alginate–reduced graphene oxide (SA-RGO) carriers, depending on the process temperature, with a particular emphasis on the temperature close to the mainstream of wastewater entering the WWTP. The RGO addition to the SA matrix causes suppression of the beads swelling, which intern modifies the mechanical properties of the gel beads. The effect of the temperature drop on the nitrogen removal rate was reduced for biomass entrapped in SA and SA-RGO gel beads in comparison to non-immobilized biomass, this suggests a ‘‘protective” effect caused by immobilization. However, analyses performed using next-generation sequencing (NGS) and qPCR revealed that the microbial community composition and relative gene abundance changed significantly, after the implementation of the new process conditions. The microbial community inside the gel beads was completely remodelled, in comparison with inoculum, and denitrification contributed to the nitrogen transformation inside the beads.

Removal of methylene blue and Lead (II) via PVA/SA double cross-linked network gel beads loaded Fe3O4@KHA nanoparticles

2021 ◽  
Author(s):  
Niu Yuhua ◽  
Han Xingxing ◽  
Song Jie ◽  
Huang Liangxian
Keyword(s):  
Methylene Blue ◽  
Polyvinyl Alcohol ◽  
Sodium Alginate ◽  
Potassium Humate ◽  
Gel Beads ◽  
Ferroferric Oxide ◽  
Double Cross

Novel magnetic gel beads were successfully fabricated via polyvinyl alcohol (PVA) and sodium alginate (SA) double cross-linked network loaded ferroferric oxide@potassium humate (Fe3O4@KHA) nanoparticles. PVA/SA/Fe3O4@KHA gel beads were found to...

scholarly journals Start-up Strategies for Anaerobic Ammonia Oxidation (Anammox) in In-Situ Nitrogen Removal from Polluted Groundwater in Rare Earth Mining Areas

2021 ◽  
Vol 13 (8) ◽  
pp. 4591
Author(s):  
Shuanglei Huang ◽  
Daishe Wu
Keyword(s):  
Rare Earth ◽  
Low Temperature ◽  
Removal Efficiency ◽  
Nitrogen Removal ◽  
Ex Situ ◽  
High Concentration ◽  
Low Concentration ◽  
Start Up ◽  
Anammox Activity

The tremendous input of ammonium and rare earth element (REE) ions released by the enormous consumption of (NH4)2SO4 in in situ leaching for ion-adsorption RE mining caused serious ground and surface water contamination. Anaerobic ammonium oxidation (anammox) was a sustainable in situ technology that can reduce this nitrogen pollution. In this research, in situ, semi in situ, and ex situ method of inoculation that included low-concentration (0.02 mg·L−1) and high-concentration (0.10 mg·L−1) lanthanum (La)(III) were adopted to explore effective start-up strategies for starting up anammox reactors seeded with activated sludge and anammox sludge. The reactors were refrigerated for 30 days at 4 °C to investigate the effects of La(III) during a period of low-temperature. The results showed that the in situ and semi in situ enrichment strategies with the addition of La(III) at a low-concentration La(III) addition (0.02 mg·L−1) reduced the length of time required to reactivate the sludge until it reached a state of stable anammox activity and high nitrogen removal efficiency by 60–71 days. The addition of La(III) promoted the formation of sludge floc with a compact structure that enabled it to resist the adverse effects of low temperature and so to maintain a high abundance of AnAOB and microbacterial community diversity of sludge during refrigeration period. The addition of La(III) at a high concentration caused the cellular percentage of AnAOB to decrease from 54.60 ± 6.19% to 17.35 ± 6.69% during the enrichment and reduced nitrogen removal efficiency to an unrecoverable level to post-refrigeration.

scholarly journals Evaluation of a novel low-carbon to nitrogen- and temperature-tolerant simultaneously nitrifying–denitrifying bacterium and its use in the treatment of river water

RSC Advances ◽  
2018 ◽  
Vol 8 (48) ◽  
pp. 27417-27422 ◽  
Author(s):  
Peng Jin ◽  
Yinyan Chen ◽  
Zhanwang Zheng ◽  
Qizhen Du
Keyword(s):  
Low Temperature ◽  
River Water ◽  
Removal Efficiency ◽  
Nitrogen Removal ◽  
Simultaneous Nitrification And Denitrification ◽  
Low Carbon ◽  
High Nitrogen ◽  
Denitrifying Bacterium ◽  
Nitrification And Denitrification

A novel simultaneous nitrification and denitrification Klebsiella sp. exhibits high nitrogen removal efficiency under low-temperature and low C/N wastewater.

scholarly journals Characterization of extracellular polymeric substances produced during nitrate removal by a thermophilic bacterium Chelatococcus daeguensis TAD1 in batch cultures

RSC Advances ◽  
2017 ◽  
Vol 7 (70) ◽  
pp. 44265-44271 ◽  
Author(s):  
Zhendong Wei ◽  
Shaobin Huang ◽  
Yongqing Zhang ◽  
Han Li ◽  
Shaofeng Zhou
Keyword(s):  
Removal Efficiency ◽  
Extracellular Polymeric Substances ◽  
Nitrate Removal ◽  
Thermophilic Bacterium ◽  
Aerobic Denitrification ◽  
Batch Cultures ◽  
Positive Correlation

Positive correlation was observed between EPS production and nitrate removal efficiency during aerobic denitrification byChelatococcus daeguensisTAD1.

Fabrication of 3D Porous Polyvinyl Alcohol/Sodium Alginate/Graphene Oxide Spherical Composites for the Adsorption of Methylene Blue

2020 ◽  
Vol 20 (4) ◽  
pp. 2205-2213 ◽  
Author(s):  
Ying-Xia Ma ◽  
Xin Li ◽  
Wen-Jie Shao ◽  
Ya-Lan Kou ◽  
Hai-Peng Yang ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Graphene Oxide ◽  
Polyvinyl Alcohol ◽  
Sodium Alginate

Synthesis and characterization of crosslinked membranes based on sodium alginate/polyvinyl alcohol/graphene oxide for ultrafiltration applications

2021 ◽  
Vol 230 ◽  
pp. 204-218
Author(s):  
Asma Rhimi ◽  
Khira Zlaoui ◽  
Bart Van der Bruggen ◽  
Karima Horchani-Naifer ◽  
Dorra Jellouli Ennigrou
Keyword(s):  
Graphene Oxide ◽  
Polyvinyl Alcohol ◽  
Sodium Alginate ◽  
Synthesis And Characterization
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