scholarly journals Multi-photon excitation in uncaging the small molecule bioregulator nitric oxide

2013 ◽  
Vol 371 (1995) ◽  
pp. 20120129 ◽  
Author(s):  
John V. Garcia ◽  
Fan Zhang ◽  
Peter C. Ford
Keyword(s):  
Nitric Oxide ◽  
Cross Sections ◽  
Near Infrared ◽  
Single Photon ◽  
Photon Absorption ◽  
Two Photon Absorption ◽  
Two Photon ◽  
Nir Light ◽  
Photon Excitation ◽  
Ultraviolet Wavelength

Multi-photon excitation allows one to use tissue transmitting near-infrared (NIR) light to access excited states with energies corresponding to single-photon excitation in the visible or ultraviolet wavelength ranges. Here, we present an overview of the application of both simultaneous and sequential multi-photon excitation in studies directed towards the photochemical delivery (‘uncaging’) of bioactive small molecules such as nitric oxide (NO) to physiological targets. Particular focus will be directed towards the use of dyes with high two-photon absorption cross sections and lanthanide ion-doped upconverting nanoparticles as sensitizers to facilitate the uncaging of NO using NIR excitation.

scholarly journals Two-photon excited deep-red and near-infrared emissive organic co-crystals

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Yu Wang ◽  
Huang Wu ◽  
Penghao Li ◽  
Su Chen ◽  
Leighton O. Jones ◽  
...  
Keyword(s):  
Cross Sections ◽  
Optical Materials ◽  
Near Infrared ◽  
Infrared Emission ◽  
Photon Absorption ◽  
Electromagnetic Spectrum ◽  
Absorption Bands ◽  
Two Photon Absorption ◽  
Two Photon ◽  
Near Infrared Emission

Abstract Two-photon excited near-infrared fluorescence materials have garnered considerable attention because of their superior optical penetration, higher spatial resolution, and lower optical scattering compared with other optical materials. Herein, a convenient and efficient supramolecular approach is used to synthesize a two-photon excited near-infrared emissive co-crystalline material. A naphthalenediimide-based triangular macrocycle and coronene form selectively two co-crystals. The triangle-shaped co-crystal emits deep-red fluorescence, while the quadrangle-shaped co-crystal displays deep-red and near-infrared emission centered on 668 nm, which represents a 162 nm red-shift compared with its precursors. Benefiting from intermolecular charge transfer interactions, the two co-crystals possess higher calculated two-photon absorption cross-sections than those of their individual constituents. Their two-photon absorption bands reach into the NIR-II region of the electromagnetic spectrum. The quadrangle-shaped co-crystal constitutes a unique material that exhibits two-photon absorption and near-infrared emission simultaneously. This co-crystallization strategy holds considerable promise for the future design and synthesis of more advanced optical materials.

Highly Conjugated, Fused-Ring, Quadrupolar Organic Chromophores with Large Two-Photon Absorption Cross-Sections in the Near-Infrared

2020 ◽  
Vol 124 (22) ◽  
pp. 4367-4378 ◽  
Author(s):  
Taylor G. Allen ◽  
Sepehr Benis ◽  
Natalia Munera ◽  
Junxiang Zhang ◽  
Shuixing Dai ◽  
...  
Keyword(s):  
Cross Sections ◽  
Near Infrared ◽  
Photon Absorption ◽  
Absorption Cross ◽  
Two Photon Absorption ◽  
Organic Chromophores ◽  
Two Photon ◽  
Fused Ring

scholarly journals Linear and nonlinear luminescence properties of thiophene based materials

2005 ◽  
Vol 70 (2) ◽  
pp. 201-208 ◽  
Author(s):  
L.U. Ping ◽  
Ming Xia
Keyword(s):  
Cross Sections ◽  
Single Photon ◽  
Photon Absorption ◽  
Luminescence Properties ◽  
Quantum Yields ◽  
Two Photon Absorption ◽  
Two Photon ◽  
Fs Laser ◽  
Thiophene Derivatives ◽  
Linear And Nonlinear

Three new thiophene based organic luminescence compounds, i.e., 2,5-bis(4?-N,N-dimethylaminostyryl) thiophene (BMST), 2,5-bis(4?-N,N-diethylaminostyryl)thiophene (BEST) and 2,5-bis(4?-N-cyclobutylaminostyryl)thiophene (BBST), were synthesized. All of their measured single-photon excited fluorescence (SPEF) are located in the range of ? 530 nm with quantum yields of around 40 %, and the corresponding lifetime was ? 1 ns. The examined compounds show strong solvatochromism in their SPEF spectra. Excited by a fs laser at 800 nm, a strong up-converted fluorescence of the examined compounds was detected. The profile of two-proton excited fluorescence (TPEF) was similar to that of SPEF. The two-photon absorption (TPA) cross sections of the compounds were determined by the TPEF method. All the compounds showed large TPA cross sections. Hence these thiophene derivatives may have good TPA properties.

scholarly journals A Series of Imidazole Derivatives: Synthesis, Two-Photon Absorption, and Application for Bioimaging

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Yingzhong Zhu ◽  
Lufei Xiao ◽  
Meng Zhao ◽  
Jiazheng Zhou ◽  
Qiong Zhang ◽  
...  
Keyword(s):  
Cross Sections ◽  
Water Solubility ◽  
Single Photon ◽  
Photon Absorption ◽  
Formation Reaction ◽  
Two Photon Absorption ◽  
Imidazole Derivatives ◽  
Two Photon ◽  
Photon Fluorescence ◽  
Imidazole Compound

A new series of D-π-A type imidazole derivatives have been synthesized and characterized. Two corresponding imidazolium salts (iodine and hexafluorophosphate) were prepared from the imidazole compound. Their electron-withdrawing ability can be largely tunable by salt formation reaction or ion exchange. UV-vis absorption and single-photon fluorescence spectra have been systematically investigated in different solvents. The two-photon cross sectionsδ2PAof the imidazole derivatives are measured by two-photon excited fluorescence (2PEF) method. Compared with those ofT-1(107 GM) andT-3(96 GM),T-2(imidazolium iodine salt) has a large two-photon absorption (2PA) cross section value of 276 GM. Furthermore, the cytotoxicity and applications in bioimaging for the imidazole derivatives were carried out. The results showed thatT-1can be used as a lysosomal tracker with high stability and water solubility within pHs of 4–6, whileT-2andT-3can be used as probes for cell cytoplasm.

scholarly journals Single cell in vivo brain optogenetic stimulation by two-photon excitation fluorescence transfer

2020 ◽  
Author(s):  
Lei Tong ◽  
Peng Yuan ◽  
Minggang Chen ◽  
Fuyi Chen ◽  
Joerg Bewersdorf ◽  
...  
Keyword(s):  
Single Cell ◽  
Cross Sections ◽  
Single Photon ◽  
Photon Absorption ◽  
Two Photon ◽  
Photon Excitation ◽  
All Optical ◽  
Stimulation Efficiency ◽  
And Control

AbstractOptogenetics at single-cell resolution can be achieved by two-photon stimulation; however, this requires intense or holographic illumination. We markedly improve stimulation efficiency by positioning fluorophores with high two-photon cross-sections adjacent to opsins. The two-photon-excited fluorescence matches the opsin absorbance and can stimulate opsins in a highly localized manner through efficient single-photon absorption. This indirect fluorescence transfer illumination allows experiments difficult to implement in the live brain such as all-optical neural interrogation and control of regional cerebral blood flow.

scholarly journals Rational design of pyrrole derivatives with aggregation-induced phosphorescence characteristics for time-resolved and two-photon luminescence imaging

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Jianhui Yang ◽  
Yahui Zhang ◽  
Xinghui Wu ◽  
Wenbo Dai ◽  
Dan Chen ◽  
...  
Keyword(s):  
Rational Design ◽  
Near Infrared ◽  
Photon Absorption ◽  
Pyrrole Derivatives ◽  
Time Resolved ◽  
Two Photon Absorption ◽  
Two Photon ◽  
Nir Light ◽  
Emission Lifetime ◽  
Good Biocompatibility

AbstractPure organic room-temperature phosphorescent (RTP) materials have been suggested to be promising bioimaging materials due to their good biocompatibility and long emission lifetime. Herein, we report a class of RTP materials. These materials are developed through the simple introduction of an aromatic carbonyl to a tetraphenylpyrrole molecule and also exhibit aggregation-induced emission (AIE) properties. These molecules show non-emission in solution and purely phosphorescent emission in the aggregated state, which are desirable properties for biological imaging. Highly crystalline nanoparticles can be easily fabricated with a long emission lifetime (20 μs), which eliminate background fluorescence interference from cells and tissues. The prepared nanoparticles demonstrate two-photon absorption characteristics and can be excited by near infrared (NIR) light, making them promising materials for deep-tissue optical imaging. This integrated aggregation-induced phosphorescence (AIP) strategy diversifies the existing pool of bioimaging agents to inspire the development of bioprobes in the future.

Two-photon absorption spectra of a near-infrared 2-azaazulene polymethine dye: solvation and ground-state symmetry breaking

2013 ◽  
Vol 15 (20) ◽  
pp. 7666 ◽  
Author(s):  
Honghua Hu ◽  
Olga V. Przhonska ◽  
Francesca Terenziani ◽  
Anna Painelli ◽  
Dmitry Fishman ◽  
...  
Keyword(s):  
Symmetry Breaking ◽  
Absorption Spectra ◽  
Ground State ◽  
Near Infrared ◽  
Photon Absorption ◽  
Two Photon Absorption ◽  
Two Photon ◽  
Polymethine Dye

Theoretical studies on the one- and two-photon absorption of tetrabenzoporphyrins and phthalocyanines

2004 ◽  
Vol 82 (1) ◽  
pp. 19-26 ◽  
Author(s):  
Xin Zhou ◽  
Ai-Min Ren ◽  
Ji-Kang Feng ◽  
Xiao-Juan Liu
Keyword(s):  
Dipole Moment ◽  
Cross Sections ◽  
Density Functional ◽  
Transition Dipole Moment ◽  
Photon Absorption ◽  
Transition Dipole ◽  
Two Photon Absorption ◽  
Two Photon ◽  
Order Of Magnitude ◽  
The One

The one-photon absorption (OPA) properties of tetrabenzoporphyrins (TBPs) and phthalocyanines (Pcs) were studied using the semiempirical ZINDO method and time-dependent density functional theory (TDDFT), respectively. The compared results confirmed that the semiempirical ZINDO method was reasonably reliable when calculating the OPA of tetrabenzoporphyrins and phthalocyanines. On the basis of the OPA properties obtained from the ZINDO method, two-photon absorption (TPA) properties of two series of molecules were investigated, using ZINDO and sum-over-states (SOS) methods. The results showed that the TPA cross-sections of all molecules were in the range of 220.6 × 10–50 – 345.9 × 10–50 cm4·s·photon–1, which were in the same order of magnitude as the values reported in the literature. The relatively larger δ(ω) value for Pcs with respect to that for corresponding TBPs originates from larger intramolecular charge transfer, which can be characterized by the difference of dipole moment between S0 and S1 and the transition dipole moment between S1 and S5.Key words: two-photon absorption, ZINDO, sum-over-states, tetrabenzoporphyrin, phthalocyanines.

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