Single cell in vivo brain optogenetic stimulation by two-photon excitation fluorescence transfer
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AbstractOptogenetics at single-cell resolution can be achieved by two-photon stimulation; however, this requires intense or holographic illumination. We markedly improve stimulation efficiency by positioning fluorophores with high two-photon cross-sections adjacent to opsins. The two-photon-excited fluorescence matches the opsin absorbance and can stimulate opsins in a highly localized manner through efficient single-photon absorption. This indirect fluorescence transfer illumination allows experiments difficult to implement in the live brain such as all-optical neural interrogation and control of regional cerebral blood flow.
2013 ◽
Vol 371
(1995)
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pp. 20120129
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2000 ◽
2005 ◽
Vol 70
(2)
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pp. 201-208
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