Control of photosynthetic carbon fixation and partitioning: how can use of genetically manipulated plants improve the nature and quality of information about regulation?

1993 ◽  
Vol 342 (1301) ◽  
pp. 225-233 ◽  

The study of regulation has previously involved indirect, and largely correlative, approaches. In the following contribution I illustrate the uses and limitations of these traditional approaches, and then discuss how molecular genetics provide a new tool to test directly ideas about regulation in vivo in the intact organism.

Author(s):  
Gunnel Karlsson ◽  
Jan-Olov Bovin ◽  
Michael Bosma

RuBisCO (D-ribulose-l,5-biphosphate carboxylase/oxygenase) is the most aboundant enzyme in the plant cell and it catalyses the key carboxylation reaction of photosynthetic carbon fixation, but also the competing oxygenase reaction of photorespiation. In vitro crystallized RuBisCO has been studied earlier but this investigation concerns in vivo existance of RuBisCO crystals in anthers and leaves ofsugarbeets. For the identification of in vivo protein crystals it is important to be able to determinethe unit cell of cytochemically identified crystals in the same image. In order to obtain the best combination of optimal contrast and resolution we have studied different staining and electron accelerating voltages. It is known that embedding and sectioning can cause deformation and obscure the unit cell parameters.


Taxon ◽  
1975 ◽  
Vol 24 (1) ◽  
pp. 27-33 ◽  
Author(s):  
Grady L. Webster ◽  
Walter V. Brown ◽  
Bruce N. Smith

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