scholarly journals Notes: Listeria welshimeri sp. nov. and Listeria seeligeri sp. nov.

1983 ◽  
Vol 33 (4) ◽  
pp. 866-869 ◽  
Author(s):  
J. ROCOURT ◽  
P. A. D. GRIMONT
Keyword(s):  
Listeria Seeligeri ◽  
Listeria Welshimeri

Virulence of Listeria monocytogenes Serovars and Listeria spp. in Experimental Infection of Mice

1991 ◽  
Vol 54 (12) ◽  
pp. 917-921 ◽  
Author(s):  
ALAIN MENUDIER ◽  
CLAUDINE BOSIRAUD ◽  
JEAN-ALBERT NICOLAS
Keyword(s):  
Listeria Monocytogenes ◽  
Peritoneal Lavage ◽  
Lavage Fluid ◽  
Fixed Number ◽  
Peritoneal Lavage Fluid ◽  
Listeria Ivanovii ◽  
Wild Strains ◽  
Camp Test ◽  
Listeria Seeligeri ◽  
Listeria Welshimeri

Wild strains of Listeria monocytogenes, Listeria ivanovii, Listeria seeligeri, Listeria innocua, and Listeria welshimeri were isolated from infected animals and foodstuffs. Their virulence was tested in Swiss mice after intraperitoneal injection of a fixed number of organisms. The presence of hemolysin was determined using the CAMP test. Bacteria were enumerated in peritoneal lavage fluid, liver, and spleen. Spleen weights were measured, and the presence of L. monocytogenes in the brain was also investigated. L. innocua, L. seeligeri, and L. welshimeri were not found to be pathogenic for mice. L. ivanovii was detected in liver, spleen, and peritoneal lavage fluid but at lower levels than L. monocytogenes (p<0.001). The pathogenic capabilities of four different serovars of L. monocytogenes (4b, 1/2a, 1/2b, 1/2c) were compared. Serovars l/2b and l/2c, which are frequently isolated from foodstuffs, were found to colonize the liver and spleen to a lesser extent than serovar 4b (p<0.01 and <0.001 respectively). The behavior of serovar l/2a, the most commonly isolated from foodstuffs, was strain dependent. Two out of the four strains tested were strongly hemolytic and were as virulent as strains of serovar 4b, while the other two were weakly hemolytic, and avirulent like L. innocua. These results could account for the relatively small number of human Listeria infections due to L. monocytogenes serogroup 1/2, despite the very frequent occurrence of this serovar in foodstuffs.

Listeria monocytogenes and Listeria spp. Contamination Patterns in Retail Delicatessen Establishments in Three U.S. States

2014 ◽  
Vol 77 (11) ◽  
pp. 1929-1939 ◽  
Author(s):  
COURTENAY SIMMONS ◽  
MATTHEW J. STASIEWICZ ◽  
EMILY WRIGHT ◽  
STEVEN WARCHOCKI ◽  
SHERRY ROOF ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Phase I ◽  
Phase Ii ◽  
Control Strategies ◽  
Food Contact ◽  
Processing Plants ◽  
Contact Surfaces ◽  
Listeria Seeligeri ◽  
Listeria Welshimeri ◽  
Further Development

Postprocessing contamination in processing plants has historically been a significant source of Listeria monocytogenes in ready-to-eat delicatessen meats, and therefore a major cause of human listeriosis cases and outbreaks. Recent risk assessments suggest that a majority of human listeriosis cases linked to consumption of contaminated deli meats may be due to L. monocytogenes contamination that occurs at the retail level. To better understand the ecology and transmission of Listeria spp. in retail delicatessens, food and nonfood contact surfaces were tested for L. monocytogenes and other Listeria spp. in a longitudinal study conducted in 30 retail delis in three U.S. states. In phase I of the study, seven sponge samples were collected monthly for 3 months in 15 delis (5 delis per state) prior to start of daily operation; in phase II, 28 food contact and nonfood contact sites were sampled in each of 30 delis during daily operation for 6 months. Among the 314 samples collected during phase I, 6.8% were positive for L. monocytogenes. Among 4,503 samples collected during phase II, 9.5% were positive for L. monocytogenes; 9 of 30 delis showed low L. monocytogenes prevalence (<1%) for all surfaces. A total of 245 Listeria spp. isolates, including 184 Listeria innocua, 48 Listeria seeligeri, and 13 Listeria welshimeri were characterized. Pulsed-field gel electrophoresis (PFGE) was used to characterize 446 L. monocytogenes isolates. PFGE showed that for 12 of 30 delis, one or more PFGE types were isolated on at least three separate occasions, providing evidence for persistence of a given L. monocytogenes subtype in the delis. For some delis, PFGE patterns for isolates from nonfood contact surfaces were distinct from patterns for occasional food contact surface isolates, suggesting limited cross-contamination between these sites in some delis. This study provides longitudinal data on L. monocytogenes contamination patterns in retail delis, which should facilitate further development of control strategies in retail delis.

Monoclonal Antibodies Directed Against the Flagellar Antigens of Listeria Species and Their Potential in EIA-Based Methods

1987 ◽  
Vol 50 (6) ◽  
pp. 479-484 ◽  
Author(s):  
JEFFREY M. FARBER ◽  
JOAN I. SPEIRS
Keyword(s):  
Staphylococcus Aureus ◽  
Monoclonal Antibodies ◽  
Listeria Monocytogenes ◽  
Enzyme Immunoassay ◽  
Listeria Innocua ◽  
Milk Samples ◽  
Listeria Ivanovii ◽  
Flagellar Antigen ◽  
Listeria Seeligeri ◽  
Listeria Welshimeri

Monoclonal antibodies directed against antigens of Listeria spp. were produced. Three main classes of immunoglobulins were found that reacted with Listeria strains containing either the A, B, or C flagellar antigen. These antibodies reacted with Listeria monocytogenes, Listeria welshimeri, Listeria seeligeri, Listeria ivanovii and Listeria innocua, but not Listeria grayi, Listeria murrayi or Listeria denitrificans. The monoclones tested did not cross-react with any of the 30 non-Listeria cultures examined, including Staphylococcus aureus and Streptococcus faecalis. Cheese and milk samples naturally-contaminated with L. monocytogenes were found to be positive for Listeria within two working days after initiation by using the monoclonal antibodies in an enzyme immunoassay.

Listeria Species in Raw and Ready-to-Eat Foods from Restaurants

2001 ◽  
Vol 64 (4) ◽  
pp. 551-553 ◽  
Author(s):  
J. M. SORIANO ◽  
H. RICO ◽  
J. C. MOLTÓ ◽  
J. MAÑES
Keyword(s):  
Listeria Monocytogenes ◽  
Listeria Innocua ◽  
Listeria Species ◽  
Listeria Ivanovii ◽  
Listeria Seeligeri ◽  
Listeria Welshimeri

From September 1999 to March 2000, meat (pork, beef, and chicken), fish (salmon, hake, and sole), vegetable (lettuce and spinach), and Spanish potato omelette samples obtained at restaurants were collected and tested for the occurrence of Listeria spp. Listeria monocytogenes was isolated from 3 (2.9%) out of 103 studied samples. Other species isolated were Listeria grayi (13.6%), Listeria innocua (1.9%), Listeria ivanovii (5.8%), Listeria seeligeri (3.9%), and Listeria welshimeri (1.9%). Listeria was neither isolated from beef nor any type of fish.

Isolation de micro-organismes du genre Listeria à partir de lait cru destiné à la consommation humaine

1985 ◽  
Vol 31 (10) ◽  
pp. 938-941 ◽  
Author(s):  
Lucas Domínguez Rodriguez ◽  
Jose F. Fernández Garayzabal ◽  
Jose A. Vazquez Boland ◽  
Elias Rodriguez Ferri ◽  
Guillermo Suarez Fernández
Keyword(s):  
Listeria Monocytogenes ◽  
Raw Milk ◽  
Listeria Innocua ◽  
Central Spain ◽  
Listeria Ivanovii ◽  
Lait Cru ◽  
Listeria Seeligeri ◽  
Listeria Welshimeri

Refrigerated mixtures of raw milk provided by a dairy which was supplied by farms from west and central Spain were tested for the presence of Listeria microorganisms. A total of 95 samples were taken at regular intervals over a 16-month period. Listeria grayi was isolated from 89.5% of the samples, Listeria monocytogenes s. str. from 45.3%, Listeria innocua from 15.8%, Listeria welshimeri from 3.1%, and Listeria seeligeri from 1.05%. Listeria ivanovii, Listeria murrayi, and Listeria denitrificans were not isolated.[Translated by the journal]

scholarly journals Isolation and molecular characterization of Listeria spp. from animals, food and environmental samples

2011 ◽  
Vol 56 (No. 8) ◽  
pp. 386-394 ◽  
Author(s):  
E. Atil ◽  
HB Ertas ◽  
G. Ozbey
Keyword(s):  
Restriction Enzymes ◽  
Feed Water ◽  
Listeria Ivanovii ◽  
Pcr Rflp ◽  
Bulk Tank ◽  
Listeria Seeligeri ◽  
Listeria Welshimeri ◽  
Pcr Techniques ◽  
Rflp Typing

A total of 46 Listeria spp. were isolated from 719 samples (milk, bulk tank swabs, cheese, feed, water, faeces and the environment) collected from 415 cattle and 304 sheep over 12 months (from February 2007 to January 2008). These isolates were identified by conventional and PCR techniques as belonging to L. monocytogenes (17.4%), to Listeria innocua (39.1%), to Listeria seeligeri 17.4%), to Listeria grayi (15.2%) and to Listeria welshimeri (11%). No Listeria ivanovii were isolated from any of the samples. Listeria spp. were not isolated from cheese and bulk tank swabs. With regard to seasonal variations most Listeria spp. were isolated in the spring and winter seasons. The eight L. monocytogenes isolates were characterized by PCR-RFLP with AluI and Tsp509I. RFLP typing of the isolates revealed two different profiles with both restriction enzymes. Four and six different profiles were produced in the examination of L. monocytogenes isolates with RAPD analysis with HLWL74 and HLWL85 primers, respectively. This is the first report on the genotyping of L. monocytogenes isolates from various sources in Turkey. This study has highlighted the need for improved control and epidemiologic strategies to prevent the transmission of Listeria spp. to animals and humans.

Incidence of Listeria monocytogenes and other Listeria spp. in Ewes' Raw Milk

1994 ◽  
Vol 57 (7) ◽  
pp. 571-575 ◽  
Author(s):  
JOSE L. RODRIGUEZ ◽  
PILAR GAVA ◽  
MARGARITA MEDINA ◽  
MANUEL NUÑEZ
Keyword(s):  
Listeria Monocytogenes ◽  
Sampling Period ◽  
Raw Milk ◽  
Seasonal Influence ◽  
Listeria Ivanovii ◽  
Milk Contamination ◽  
One Year ◽  
The One ◽  
Listeria Seeligeri ◽  
Listeria Welshimeri

Ewes' milk samples from 287 farm bulk tanks and 17 transport tankers were analyzed for Listeria over a one-year period. Listeria monocytogenes and Listeria innocua were detected in 2.19% and 2.00% of 1052 farm samples, and in 18.38% and 11.76% of 136 tanker samples, respectively. Incidence of Listeria grayi, Listeria ivanovii, Listeria seeligeri and Listeria welshimeri was under 0.4% in farm samples and under 1% in tanker samples. Most farms (93.38%) produced milk free from L. monocytogenes throughout the one-year sampling period. No seasonal influence on milk contamination by Listeria was found. However, ewes' milk contamination by L. monocytogenes and other Listeria spp. was significantly higher in farms where cows were also reared than in farms where only ewes were present.

Prevalence and Contamination Levels of Listeria monocytogenes in Smoked Fish and Pâté Sold in Spain

2001 ◽  
Vol 64 (12) ◽  
pp. 2075-2077 ◽  
Author(s):  
C. DOMINGUEZ ◽  
I. GOMEZ ◽  
J. ZUMALACARREGUI
Keyword(s):  
Listeria Monocytogenes ◽  
Listeria Innocua ◽  
Smoked Fish ◽  
Listeria Ivanovii ◽  
Retail Outlets ◽  
Contamination Levels ◽  
Listeria Seeligeri ◽  
Listeria Welshimeri

From March to November 2000, 170 samples of smoked fish and 182 samples of pâté for sale in retail outlets and supermarkets in the nine provinces of Castilla and León (Spain) were analyzed for the prevalence of Listeria monocytogenes and other Listeria spp. L. monocytogenes was isolated from 38 (22.3%) of the 170 samples of smoked fish analyzed. Twenty of these positive samples contained L. monocytogenes at >100 CFU/g. Other Listeria spp., such as Listeria innocua (26 isolates), Listeria grayi (9), Listeria welshimeri (3), Listeria seeligeri (3), and Listeria ivanovii (2), were also detected. L. monocytogenes was isolated from 5.4% of the 182 samples of pâté. Only 1 of the 10 positive samples harbored >100 L. monocytogenes CFU/g. Two other species of Listeria were observed in pâté: L. innocua (12 isolates) and L. grayi (2).

Prevalence and Antimicrobial Resistance of Listeria Species Isolated from Different Types of Raw Meat in Iran

2012 ◽  
Vol 75 (12) ◽  
pp. 2223-2227 ◽  
Author(s):  
EBRAHIM RAHIMI ◽  
FARZAD YAZDI ◽  
HUSSEIN FARZINEZHADIZADEH
Keyword(s):  
Foodborne Pathogens ◽  
The Elderly ◽  
Common Species ◽  
Antimicrobial Drugs ◽  
Raw Meat ◽  
Disk Diffusion Assay ◽  
Meat Samples ◽  
Listeria Seeligeri ◽  
Listeria Welshimeri ◽  
Diffusion Assay

Listeria and particularly Listeria monocytogenes are important foodborne pathogens that can cause listeriosis and severe complications in immunocompromised individuals, children, pregnant women, and the elderly. The objective of this study was to determine the prevalence of Listeria spp. in raw meat in Iran. From July 2010 to November 2011, a total of 1,107 samples of various raw meats were obtained from randomly selected retail butcher shops. The results of conventional bacteriologic and PCR methods revealed that 141 samples (12.7%) were positive for Listeria spp. The highest prevalence of Listeria was found in raw buffalo meat samples (7 of 24 samples; 29.2%) followed by quail meat (26 of 116 samples; 22.4%), partridge meat (13 of 74 samples; 17.6%), and chicken meat (27 of 160 samples; 16.9%). The most common species recovered was Listeria innocua (98 of 141 strains; 75.9%); the remaining isolates were L. monocytogenes (19.1% of strains), Listeria welshimeri (6.4% of strains), Listeria seeligeri (3.5% of strains), and Listeria grayi (1.4% of strains). Susceptibilities of the 141 strains to 11 antimicrobial drugs were determined using the disk diffusion assay. Overall, 104 (73.8%) of the Listeria isolates were resistant to one or more antimicrobials, and 17.0% of the isolates were resistant to three or more antimicrobials. The present study provides the first baseline data on the prevalence of Listeria in raw meat derived from sheep, goat, buffalo, quail, partridge, chicken, and ostrich in Iran and the susceptibility of these isolates to antimicrobials.

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