Variation in the Bluetongue Virus Neutralization Protein VP2

Using antigens prepared from cell cultures infected by bluetongue (BLU) virus type 20 (BLU-20), and sera from cattle which had recovered from experimental infection by that virus, two distinct precipitin reactions were demonstrated by immunodiffusion. Two distinct gel diffusion precipitin tests were developed based on these reactions. The antigen of one was common to BLU-20 and two other Australian BLU isolates, CSIRO 154 (BLU-21) and CSIRO 156 (BLU-l). It was therefore concluded to be a group-specific test. The antigen of the second appeared to be unique to BLU-20. The test based on this antigen correlated well with the virus neutralization test for BLU-20 and it was therefore concluded to be type-specific.

Download Full-text

Identification of bluetongue virus type 17 genome segments coding for polypeptides associated with virus neutralization and intergroup reactivity

Virology ◽

10.1016/0042-6822(83)90503-2 ◽

1983 ◽

Vol 131 (2) ◽

pp. 355-366 ◽

Cited By ~ 53

Author(s):

Marvin J. Grubman ◽

Judith A. Appleton ◽

Geoffrey J. Letchworth

Keyword(s):

Virus Type ◽

Bluetongue Virus ◽

Virus Neutralization

Download Full-text

Exposure of protein VP7 on the surface of bluetongue virus

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100160558 ◽

1990 ◽

Vol 48 (3) ◽

pp. 600-601

Author(s):

A.D. Hyatt

Keyword(s):

Bluetongue Virus ◽

Structural Proteins ◽

Major Constituent ◽

Outer Coat ◽

Virus Particles ◽

Antibody Recognition ◽

The Core ◽

The Family ◽

Electron Microscopical ◽

Physical Accessibility

Bluetongue virus (BTV) is the type species os the genus orbivirus in the family Reoviridae. The virus has a fibrillar outer coat containing two major structural proteins VP2 and VP5 which surround an icosahedral core. The core contains two major proteins VP3 and VP7 and three minor proteins VP1, VP4 and VP6. Recent evidence has indicated that the core comprises a neucleoprotein center which is surrounded by two protein layers; VP7, a major constituent of capsomeres comprises the outer and VP3 the inner layer of the core . Antibodies to VP7 are currently used in enzyme-linked immunosorbant assays and immuno-electron microscopical (JEM) tests for the detection of BTV. The tests involve the antibody recognition of VP7 on virus particles. In an attempt to understand how complete viruses can interact with antibodies to VP7 various antibody types and methodologies were utilized to determine the physical accessibility of the core to the external environment.

Download Full-text

Umatilla Virus Association with Fibrils and Tubules in Cultured Cells

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s1431927600002063 ◽

1980 ◽

Vol 38 ◽

pp. 476-477

Author(s):

Neil M. Foster ◽

Ruth D. Breckon

Keyword(s):

Bluetongue Virus ◽

Linear Array ◽

Cultured Cells ◽

Intimate Association ◽

Infected Cells ◽

Dark Staining ◽

In Cells

Macrotubules have been described1 in cells infected with Umatilla virus (UMAV), an orbivirus for which bluetongue virus (BTV) is the protype. Macrotubules, often in linear array, were observed in the cytoplasm and in intimate association with viroplasms of infected cells. Macrotubules had outside and inside diameters of 20 and 15 nm and many had dark-staining centers with diameters similar to the interiors of the tubules. UMAV was 60 nm and the RNA core was 30 nm in diameter. This report describes the association of UMAV with macrotubules and two types of microtubules.

Download Full-text