scholarly journals In vitro decapsidation of turnip yellow mosaic virus investigated by cryo-electron microscopy: a model for the decapsidation of a small isometric virus

1992 ◽  
Vol 73 (8) ◽  
pp. 2079-2083 ◽  
Author(s):  
M. Adrian ◽  
P. A. Timmins ◽  
J. Witz
Keyword(s):  
Electron Microscopy ◽  
Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Turnip Yellow Mosaic Virus ◽  
Cryo Electron Microscopy

A note on the electron microscopy of the turnip yellow mosaic virus

Parasitology ◽  
1953 ◽  
Vol 43 (3-4) ◽  
pp. 191-192 ◽  
Author(s):  
Kenneth M. Smith
Keyword(s):  
Electron Microscopy ◽  
Electron Microscope ◽  
Mosaic Virus ◽  
Chinese Cabbage ◽  
Hollow Spheres ◽  
Yellow Mosaic Virus ◽  
Turnip Yellow Mosaic Virus ◽  
Hollow Particles ◽  
Brassica Chinensis ◽  
Virus Particles

An attempt to differentiate by means of the electron microscope between particles of the top and bottom components of the turnip yellow mosaic virus is described. Since the particles of the top component are thought to be hollow spheres, it might be expected that they would collapse after drying and thus throw a smaller shadow than the particles of the bottom component which are thought to have a more solid centre. No difference could be perceived between the two types of particle, so that the apparently hollow particles can withstand the effect of drying without collapsing.Sections were cut of leaves of Chinese cabbage (Brassica chinensis) and particles were observed inside the cells which could conceivably be virus particles.

scholarly journals ELECTRON MICROSCOPY OF STRUCTURAL DETAIL IN FROZEN BIOLOGICAL SPECIMENS

1957 ◽  
Vol 3 (1) ◽  
pp. 45-60 ◽  
Author(s):  
Russell L. Steere
Keyword(s):  
Electron Microscopy ◽  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Internal Structure ◽  
Plant Virus ◽  
Yellow Mosaic Virus ◽  
Turnip Yellow Mosaic Virus ◽  
Structural Detail ◽  
Hexagonal Packing

A procedure is described whereby preshadowed replicas can be obtained from frozen biological specimens which have been cut and then etched by sublimation of the ice from their surfaces. Electron micrographs showing details of the internal structure of plant virus crystals are presented to demonstrate the values of the procedure. Crystals of purified tobacco ringspot virus and squash mosaic virus and some portions of turnip yellow mosaic virus crystals have been shown to exhibit hexagonal packing. Sections through in situ crystals of tobacco mosaic virus show the rods to be parallel within each layer and arranged in a square net as viewed end on. Individual rods in each layer of the latter measure 300 mµ in length and are somewhat tilted with respect to the rods of adjacent layers. This results in the formation of a herring-bone appearance when a crystal is cut perpendicular to its hexagonal face. It is suggested that the procedure outlined here might well serve to supplement other procedures for the preparation of many cytological specimens for electron microscopy.

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