scholarly journals Common evolutionary origin of aquareoviruses and orthoreoviruses revealed by genome characterization of Golden shiner reovirus, Grass carp reovirus, Striped bass reovirus and golden ide reovirus (genus Aquareovirus, family Reoviridae)

2002 ◽  
Vol 83 (8) ◽  
pp. 1941-1951 ◽  
Author(s):  
Houssam Attoui ◽  
Qin Fang ◽  
Fauziah Mohd Jaafar ◽  
Jean-François Cantaloube ◽  
Philippe Biagini ◽  
...  
Keyword(s):  
Striped Bass ◽  
Grass Carp ◽  
Genetic Relatedness ◽  
Amino Acid Identity ◽  
Evolutionary Origin ◽  
Antigenic Relationship ◽  
Grass Carp Reovirus ◽  
Common Evolutionary Origin ◽  
Golden Shiner ◽  
The Status

Full-length and partial genome sequences of four members of the genus Aquareovirus, family Reoviridae (Golden shiner reovirus, Grass carp reovirus, Striped bass reovirus and golden ide reovirus) were characterized. Based on sequence comparison, the unclassified Grass carp reovirus was shown to be a member of the species Aquareovirus C. The status of golden ide reovirus, another unclassified aquareovirus, was also examined. Sequence analysis showed that it did not belong to the species Aquareovirus A or C, but assessment of its relationship to the species Aquareovirus B, D, E and F was hampered by the absence of genetic data from these species. In agreement with previous reports of ultrastructural resemblance between aquareoviruses and orthoreoviruses, genetic analysis revealed homology in the genes of the two groups. This homology concerned eight of the 11 segments of the aquareovirus genome (amino acid identity 17–42%), and similar genetic organization was observed in two other segments. The conserved terminal sequences in the genomes of members of the two groups were also similar. These data are undoubtedly an indication of the common evolutionary origin of these viruses. This clear genetic relatedness between members of distinct genera is unique within the family Reoviridae. Such a genetic relationship is usually observed between members of a single genus. However, the current taxonomic classification of aquareoviruses and orthoreoviruses in two different genera is supported by a number of characteristics, including their distinct G+C contents, unequal numbers of genome segments, absence of an antigenic relationship, different cytopathic effects and specific econiches.

scholarly journals Identification, Virulence, and Molecular Characterization of a Recombinant Isolate of Grass Carp Reovirus Genotype I

Viruses ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 807
Author(s):  
Weiwei Zeng ◽  
Sven M. Bergmannc ◽  
Hanxu Dong ◽  
Ying Yang ◽  
Minglin Wu ◽  
...  
Keyword(s):  
Grass Carp ◽  
Genomic Sequence ◽  
Electrophoretic Pattern ◽  
Amino Acid Identity ◽  
The Body ◽  
Hemorrhagic Disease ◽  
Isolation And Identification ◽  
Genotype I ◽  
Grass Carp Reovirus ◽  
Rare Minnow

The hemorrhagic disease of grass carp (HDGC) caused by grass carp reovirus (GCRV) still poses a great threat to the grass carp industry. Isolation and identification of the GCRV genotype I (GCRV-I) has been rarely reported in the past decade. In this study, a new GCRV was isolated from diseased fish with severe symptoms of enteritis and mild hemorrhages on the body surface. The isolate was further identified by cell culture, transmission electron, indirect immunofluorescence, and SDS-PAGE electrophoretic pattern analysis of genomic RNA. The results were consistent with the new isolate as a GCRV-I member and tentatively named GCRV-GZ1208. Both grass carp and rare minnow infected by the GCRV-GZ1208 have no obvious hemorrhagic symptoms, and the final mortality rate was ≤10%, indicating that it may be a low virulent isolate. GZ1208 possessed highest genomic homology to 873/GCHV (GCRV-I) and golden shiner reovirus (GSRV). Additionally, it was found a 90.7–98.3% nucleotide identity, a 96.4–100% amino acid identity, and <50% identity with GCRV-II and III genotypes. Interestingly, the sequences of some segments of GZ1208 were similar to GCRV-8733/GCHV, whereas the remaining segments were more closely related to GSRV, suggesting that a recombination event had occurred. Bootscan analysis of the complete genomic sequence confirmed this hypothesis, and recombination events between 873/GCHV and other GSRV-like viruses were also accompanied by gene mutations.

RESISTANCE OF SIGCRV TRANSGENIC RARE MINNOW(GOBIOCYPRIS RARUS)TO GRASS CARP REOVIRUS

2010 ◽  
Vol 36 (4) ◽  
pp. 837-842 ◽  
Author(s):  
Sha LIAO ◽  
Yun CHEN ◽  
Fu-Kuan DU ◽  
Ya-Ping WANG ◽  
Lan-Jie LIAO ◽  
...  
Keyword(s):  
Grass Carp ◽  
Gobiocypris Rarus ◽  
Grass Carp Reovirus ◽  
Rare Minnow

Establishment of multiplex PCR for detection of grass carp reovirus and its application

2013 ◽  
Vol 20 (2) ◽  
pp. 419-426 ◽  
Author(s):  
Weiwei ZENG ◽  
Qing WANG ◽  
Yingying WANG ◽  
Lesheng ZHANG ◽  
Baoqin LIU ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Grass Carp ◽  
Grass Carp Reovirus

Isolation and identification of grass carp reovirus strain JX-0902

2013 ◽  
Vol 18 (5) ◽  
pp. 1077-1083 ◽  
Author(s):  
Yongkui LIU ◽  
Qing WANG ◽  
Weiwei ZENG ◽  
Cunbin SHI ◽  
Chao ZHANG ◽  
...  
Keyword(s):  
Grass Carp ◽  
Isolation And Identification ◽  
Grass Carp Reovirus

Preparation and identification of monoclonal antibody against VP4 protein of grass carp reovirus HZ08 strain

2013 ◽  
Vol 37 (3) ◽  
pp. 450 ◽  
Author(s):  
Weiwei ZENG ◽  
Qing WANG ◽  
Yingying WANG ◽  
Cunbin SHI ◽  
Shuqin WU
Keyword(s):  
Monoclonal Antibody ◽  
Grass Carp ◽  
Grass Carp Reovirus

Development and efficacy of a grass carp reovirus (GCRV) outer capsid protein VP7 subunit vaccine

2012 ◽  
Vol 37 (6) ◽  
pp. 659-664 ◽  
Author(s):  
Shi-ying XU ◽  
Jing-hui LI ◽  
Yong ZOU ◽  
Lin LIU ◽  
Cheng-liang GONG ◽  
...  
Keyword(s):  
Capsid Protein ◽  
Grass Carp ◽  
Subunit Vaccine ◽  
Outer Capsid Protein ◽  
Grass Carp Reovirus ◽  
Outer Capsid

scholarly journals Oral Vaccination of Grass Carp (Ctenopharyngodon idella) with Baculovirus-Expressed Grass Carp Reovirus (GCRV) Proteins Induces Protective Immunity against GCRV Infection

Vaccines ◽  
2021 ◽  
Vol 9 (1) ◽  
pp. 41
Author(s):  
Changyong Mu ◽  
Qiwang Zhong ◽  
Yan Meng ◽  
Yong Zhou ◽  
Nan Jiang ◽  
...  
Keyword(s):  
Survival Rate ◽  
Grass Carp ◽  
Oral Vaccine ◽  
Serum Antibody ◽  
Ctenopharyngodon Idella ◽  
Control Group ◽  
Oral Vaccination ◽  
Grass Carp Reovirus ◽  
Silkworm Pupae ◽  
Grass Carp Ctenopharyngodon Idella

The grass carp reovirus (GCRV) causes severe hemorrhagic disease with high mortality and leads to serious economic losses in the grass carp (Ctenopharyngodon idella) industry in China. Oral vaccine has been proven to be an effective method to provide protection against fish viruses. In this study, a recombinant baculovirus BmNPV-VP35-VP4 was generated to express VP35 and VP4 proteins from GCRV type Ⅱ via Bac-to-Bac baculovirus expression system. The expression of recombinant VP35-VP4 protein (rVP35-VP4) in Bombyx mori embryo cells (BmE) and silkworm pupae was confirmed by Western blotting and immunofluorescence assay (IFA) after infection with BmNPV-VP35-VP4. To vaccinate the grass carp by oral route, the silkworm pupae expressing the rVP35-VP4 proteins were converted into a powder after freeze-drying, added to artificial feed at 5% and fed to grass carp (18 ± 1.5 g) for six weeks, and the immune response and protective efficacy in grass carp after oral vaccination trial was thoroughly investigated. This included blood cell counting and classification, serum antibody titer detection, immune-related gene expression and the relative percent survival rate in immunized grass carp. The results of blood cell counts show that the number of white blood cells in the peripheral blood of immunized grass carp increased significantly from 14 to 28 days post-immunization (dpi). The differential leukocyte count of neutrophils and monocytes were significantly higher than those in the control group at 14 dpi. Additionally, the number of lymphocytes increased significantly and reached a peak at 28 dpi. The serum antibody levels were significantly increased at Day 14 and continued until 42 days post-vaccination. The mRNA expression levels of immune-related genes (IFN-1, TLR22, IL-1β, MHC I, Mx and IgM) were significantly upregulated in liver, spleen, kidney and hindgut after immunization. Four weeks post-immunization, fish were challenged with virulent GCRV by intraperitoneal injection. The results of this challenge study show that orally immunized group exhibited a survival rate of 60% and relative percent survival (RPS) of 56%, whereas the control group had a survival rate of 13% and RPS of 4%. Taken together, our results demonstrate that the silkworm pupae powder containing baculovirus-expressed VP35-VP4 proteins could induce both non-specific and specific immune responses and protect grass carp against GCRV infection, suggesting it could be used as an oral vaccine.

scholarly journals Grass Carp Reovirus (GCRV) Giving Its All to Suppress IFN Production by Countering MAVS Signaling Transduction

2020 ◽  
Vol 11 ◽  
Author(s):  
Long-Feng Lu ◽  
Zhuo-Cong Li ◽  
Can Zhang ◽  
Xiao-Yu Zhou ◽  
Yu Zhou ◽  
...  
Keyword(s):  
Grass Carp ◽  
Signaling Transduction ◽  
Grass Carp Reovirus
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