scholarly journals Identification, Virulence, and Molecular Characterization of a Recombinant Isolate of Grass Carp Reovirus Genotype I

Viruses ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 807
Author(s):  
Weiwei Zeng ◽  
Sven M. Bergmannc ◽  
Hanxu Dong ◽  
Ying Yang ◽  
Minglin Wu ◽  
...  
Keyword(s):  
Grass Carp ◽  
Genomic Sequence ◽  
Electrophoretic Pattern ◽  
Amino Acid Identity ◽  
The Body ◽  
Hemorrhagic Disease ◽  
Isolation And Identification ◽  
Genotype I ◽  
Grass Carp Reovirus ◽  
Rare Minnow

The hemorrhagic disease of grass carp (HDGC) caused by grass carp reovirus (GCRV) still poses a great threat to the grass carp industry. Isolation and identification of the GCRV genotype I (GCRV-I) has been rarely reported in the past decade. In this study, a new GCRV was isolated from diseased fish with severe symptoms of enteritis and mild hemorrhages on the body surface. The isolate was further identified by cell culture, transmission electron, indirect immunofluorescence, and SDS-PAGE electrophoretic pattern analysis of genomic RNA. The results were consistent with the new isolate as a GCRV-I member and tentatively named GCRV-GZ1208. Both grass carp and rare minnow infected by the GCRV-GZ1208 have no obvious hemorrhagic symptoms, and the final mortality rate was ≤10%, indicating that it may be a low virulent isolate. GZ1208 possessed highest genomic homology to 873/GCHV (GCRV-I) and golden shiner reovirus (GSRV). Additionally, it was found a 90.7–98.3% nucleotide identity, a 96.4–100% amino acid identity, and <50% identity with GCRV-II and III genotypes. Interestingly, the sequences of some segments of GZ1208 were similar to GCRV-8733/GCHV, whereas the remaining segments were more closely related to GSRV, suggesting that a recombination event had occurred. Bootscan analysis of the complete genomic sequence confirmed this hypothesis, and recombination events between 873/GCHV and other GSRV-like viruses were also accompanied by gene mutations.

scholarly journals A Novel Subunit Vaccine Based on Outer Capsid Proteins of Grass Carp Reovirus (GCRV) Provides Protective Immunity against GCRV Infection in Rare Minnow (Gobiocypris rarus)

Pathogens ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 945
Author(s):  
Changyong Mu ◽  
Vikram N. Vakharia ◽  
Yong Zhou ◽  
Nan Jiang ◽  
Wenzhi Liu ◽  
...  
Keyword(s):  
Grass Carp ◽  
Subunit Vaccine ◽  
Capsid Proteins ◽  
Economic Losses ◽  
Hemorrhagic Disease ◽  
Gobiocypris Rarus ◽  
Grass Carp Reovirus ◽  
Expression Levels ◽  
Rare Minnow ◽  
Outer Capsid

The grass carp hemorrhagic disease, caused by the grass carp reovirus (GCRV), has resulted in severe economic losses in the aquaculture industry in China. VP4 and VP35 are outer capsid proteins of GCRV and can induce an immune response in the host. Here, three recombinant baculoviruses, AcMNPV-VP35, AcMNPV-VP4, and AcMNPV-VP35-VP4, were generated to express recombinant VP4 and VP35 proteins from GCRV type II in insect cells by using the Bac-to-Bac baculovirus expression system to create a novel subunit vaccine. The expression of recombinant VP35, VP4, and VP35-VP4 proteins in Sf-9 cells were confirmed by Western blotting and immunofluorescence. Recombinant VP35, VP4, and VP35-VP4 were purified from baculovirus-infected cell lysates and injected intraperitoneally (3 μg/fish) into the model rare minnow, Gobiocypris rarus. After 21 days, the immunized fish were challenged with virulent GCRV. Liver, spleen, and kidney samples were collected at different time intervals to evaluate the protective efficacy of the subunit vaccines. The mRNA expression levels of some immune-related genes detected by using quantitative real-time PCR (qRT-PCR) were significantly upregulated in the liver, spleen, and kidney, with higher expression levels in the VP35-VP4 group. The nonvaccinated fish group showed 100% mortality, whereas the VP35-VP4, VP4, and VP35 groups exhibited 67%, 60%, and 33% survival, respectively. In conclusion, our results revealed that recombinant VP35 and VP4 can induce immunity and protect against GCRV infection, with their combined use providing the best effect. Therefore, VP35 and VP4 proteins can be used as a novel subunit vaccine against GCRV infection.

RESISTANCE OF SIGCRV TRANSGENIC RARE MINNOW(GOBIOCYPRIS RARUS)TO GRASS CARP REOVIRUS

2010 ◽  
Vol 36 (4) ◽  
pp. 837-842 ◽  
Author(s):  
Sha LIAO ◽  
Yun CHEN ◽  
Fu-Kuan DU ◽  
Ya-Ping WANG ◽  
Lan-Jie LIAO ◽  
...  
Keyword(s):  
Grass Carp ◽  
Gobiocypris Rarus ◽  
Grass Carp Reovirus ◽  
Rare Minnow

Isolation and identification of grass carp reovirus strain JX-0902

2013 ◽  
Vol 18 (5) ◽  
pp. 1077-1083 ◽  
Author(s):  
Yongkui LIU ◽  
Qing WANG ◽  
Weiwei ZENG ◽  
Cunbin SHI ◽  
Chao ZHANG ◽  
...  
Keyword(s):  
Grass Carp ◽  
Isolation And Identification ◽  
Grass Carp Reovirus

Establishment of a cell line from egg of rare minnow Gobiocypris rarus for propagation of grass carp reovirus genotype II

2019 ◽  
Vol 136 ◽  
pp. 103715 ◽  
Author(s):  
Shixu Liu ◽  
Yingying Wang ◽  
Jiaming Chen ◽  
Qing Wang ◽  
Ouqin Chang ◽  
...  
Keyword(s):  
Cell Line ◽  
Grass Carp ◽  
Gobiocypris Rarus ◽  
Grass Carp Reovirus ◽  
Rare Minnow ◽  
A Cell ◽  
Genotype Ii

Transcriptome analysis of rare minnow (Gobiocypris rarus) infected by the grass carp reovirus

2019 ◽  
Vol 89 ◽  
pp. 337-344 ◽  
Author(s):  
Yusheng Lin ◽  
Bing Wang ◽  
Nenghan Wang ◽  
Gang Ouyang ◽  
Hong Cao
Keyword(s):  
Transcriptome Analysis ◽  
Grass Carp ◽  
Gobiocypris Rarus ◽  
Grass Carp Reovirus ◽  
Rare Minnow

Grass carp reovirus activates RNAi pathway in rare minnow, Gobiocypris rarus

Aquaculture ◽  
2009 ◽  
Vol 289 (1-2) ◽  
pp. 1-5 ◽  
Author(s):  
Jianguo Su ◽  
Zuoyan Zhu ◽  
Yaping Wang ◽  
Jun Zou ◽  
Na Wang ◽  
...  
Keyword(s):  
Grass Carp ◽  
Rnai Pathway ◽  
Gobiocypris Rarus ◽  
Grass Carp Reovirus ◽  
Rare Minnow

scholarly journals Grass Carp Reovirus VP41 Targets Fish MITA To Abrogate the Interferon Response

2017 ◽  
Vol 91 (14) ◽  
Author(s):  
Long-Feng Lu ◽  
Shun Li ◽  
Zhao-Xi Wang ◽  
Si-Qi Du ◽  
Dan-Dan Chen ◽  
...  
Keyword(s):  
Grass Carp ◽  
Critical Role ◽  
Adaptor Protein ◽  
Interferon Response ◽  
Hemorrhagic Disease ◽  
Grass Carp Reovirus ◽  
Antiviral Responses ◽  
Mitochondrial Antiviral Signaling ◽  
First Time ◽  
Irf3 Activation

ABSTRACT Although fish possess an efficient interferon (IFN) system to defend against aquatic virus infection, grass carp reovirus (GCRV) still causes hemorrhagic disease in grass carp. To date, GCRV's strategy for evading the fish IFN response is still unknown. Here, we report that GCRV VP41 inhibits fish IFN production by suppressing the phosphorylation of mediator of IFN regulatory factor 3 (IRF3) activation (MITA). First, the activation of the IFN promoter (IFNpro) stimulated by mitochondrial antiviral signaling protein (MAVS) and MITA was decreased by the overexpression of VP41, whereas such activation induced by TANK-binding kinase 1 (TBK1) was not affected. Second, VP41 was colocalized in the cellular endoplasmic reticulum (ER) and associated with MITA. Furthermore, as a phosphorylation substrate of TBK1, VP41 significantly decreased the phosphorylation of MITA. Truncation assays indicated that the transmembrane (TM) region of VP41 was indispensable for the suppression of IFNpro activity. Finally, after infection with GCRV, VP41 blunted the transcription of host IFN and facilitated viral RNA synthesis. Taken together, our findings suggest that GCRV VP41 prevents the fish IFN response by attenuating the phosphorylation of MITA for viral evasion. IMPORTANCE MITA is thought to act as an adaptor protein to facilitate the phosphorylation of IRF3 by TBK1 upon viral infection, and it plays a critical role in innate antiviral responses. Here, we report that GCRV VP41 colocalizes with MITA at the ER and reduces MITA phosphorylation by acting as a decoy substrate of TBK1, thus inhibiting IFN production. These findings reveal GCRV's strategy for evading the host IFN response for the first time.

scholarly journals Common evolutionary origin of aquareoviruses and orthoreoviruses revealed by genome characterization of Golden shiner reovirus, Grass carp reovirus, Striped bass reovirus and golden ide reovirus (genus Aquareovirus, family Reoviridae)

2002 ◽  
Vol 83 (8) ◽  
pp. 1941-1951 ◽  
Author(s):  
Houssam Attoui ◽  
Qin Fang ◽  
Fauziah Mohd Jaafar ◽  
Jean-François Cantaloube ◽  
Philippe Biagini ◽  
...  
Keyword(s):  
Striped Bass ◽  
Grass Carp ◽  
Genetic Relatedness ◽  
Amino Acid Identity ◽  
Evolutionary Origin ◽  
Antigenic Relationship ◽  
Grass Carp Reovirus ◽  
Common Evolutionary Origin ◽  
Golden Shiner ◽  
The Status

Full-length and partial genome sequences of four members of the genus Aquareovirus, family Reoviridae (Golden shiner reovirus, Grass carp reovirus, Striped bass reovirus and golden ide reovirus) were characterized. Based on sequence comparison, the unclassified Grass carp reovirus was shown to be a member of the species Aquareovirus C. The status of golden ide reovirus, another unclassified aquareovirus, was also examined. Sequence analysis showed that it did not belong to the species Aquareovirus A or C, but assessment of its relationship to the species Aquareovirus B, D, E and F was hampered by the absence of genetic data from these species. In agreement with previous reports of ultrastructural resemblance between aquareoviruses and orthoreoviruses, genetic analysis revealed homology in the genes of the two groups. This homology concerned eight of the 11 segments of the aquareovirus genome (amino acid identity 17–42%), and similar genetic organization was observed in two other segments. The conserved terminal sequences in the genomes of members of the two groups were also similar. These data are undoubtedly an indication of the common evolutionary origin of these viruses. This clear genetic relatedness between members of distinct genera is unique within the family Reoviridae. Such a genetic relationship is usually observed between members of a single genus. However, the current taxonomic classification of aquareoviruses and orthoreoviruses in two different genera is supported by a number of characteristics, including their distinct G+C contents, unequal numbers of genome segments, absence of an antigenic relationship, different cytopathic effects and specific econiches.

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