Mutants of Bacillus subtilis 168 Thermosensitive for Growth and Wall Teichoic Acid Synthesis

Microbiology ◽  
1989 ◽  
Vol 135 (5) ◽  
pp. 1325-1334 ◽  
Author(s):  
M. Briehl ◽  
H. M. Pooley ◽  
D. Karamata
Keyword(s):  
Bacillus Subtilis ◽  
Teichoic Acid ◽  
Acid Synthesis ◽  
Wall Teichoic Acid ◽  
Bacillus Subtilis 168

scholarly journals Control of teichoic acid and teichuronic acid biosynthesis in chemostat cultures of Bacillus subtilis var. niger

1969 ◽  
Vol 111 (1) ◽  
pp. 1-5 ◽  
Author(s):  
D C Ellwood ◽  
D. W. Tempest
Keyword(s):  
Cell Wall ◽  
Bacillus Subtilis ◽  
Teichoic Acid ◽  
Extracellular Fluid ◽  
Acid Synthesis ◽  
Growth Cycle ◽  
Wall Material ◽  
Wall Teichoic Acid ◽  
Anionic Polymers ◽  
Teichuronic Acid

1. Quantitative determination of the anionic polymers present in the walls of Bacillus subtilis var. niger organisms undergoing transition, in a chemostat culture, from either Mg2+-limitation to PO43−-limitation or K+-limitation to PO43−-limitation showed that teichuronic acid synthesis started immediately the culture became PO43−-limited and proceeded at a rate substantially faster than the rate of biomass synthesis. 2. Simultaneously, the cell-wall teichoic acid content diminished at a rate greater than that due to dilution by newly synthesized wall material, and fragments of teichoic acid and mucopeptide accumulated in the culture extracellular fluid. 3. Equally rapid reverse changes occurred when a PO43−-limited B. subtilis var. niger culture was returned to being Mg2+-limited. 4. It is concluded that in this organism both teichoic acid and teichuronic acid syntheses are expressions of a single genotype, and a mechanism for the control of synthesis of both polymers is suggested. 5. These results are discussed with reference to the constantly changing environmental conditions that obtain in a batch culture and the variation in bacterial cell-wall composition that is reported to occur throughout the growth cycle.

scholarly journals Analysis of Bacillus subtilis tagAB andtagDEF Expression during Phosphate Starvation Identifies a Repressor Role for PhoP∼P

1998 ◽  
Vol 180 (3) ◽  
pp. 753-758 ◽  
Author(s):  
Wei Liu ◽  
Stephen Eder ◽  
F. Marion Hulett
Keyword(s):  
Cell Wall ◽  
Bacillus Subtilis ◽  
Teichoic Acid ◽  
Phosphate Starvation ◽  
Acid Synthesis ◽  
Pho Regulon ◽  
Wall Teichoic Acid ◽  
Negative Role ◽  
First Time ◽  
Starvation Conditions

ABSTRACT The tagAB and tagDEF operons, which are adjacent and divergently transcribed, encode genes responsible for cell wall teichoic acid synthesis in Bacillus subtilis. TheBacillus data presented here suggest that PhoP and PhoR are required for direct repression of transcription of the two operons under phosphate starvation conditions but have no regulatory role under phosphate-replete conditions. These data identify for the first time that PhoP∼P has a negative role in Pho regulon gene regulation.

Expression of heterologous genes for wall teichoic acid in Bacillus subtilis 168

1987 ◽  
Vol 207 (1) ◽  
pp. 73-81 ◽  
Author(s):  
Dimitri Karamata ◽  
Harold M. Pooley ◽  
Michel Monod
Keyword(s):  
Bacillus Subtilis ◽  
Teichoic Acid ◽  
Wall Teichoic Acid ◽  
Bacillus Subtilis 168

scholarly journals Wall Teichoic Acid Polymers Are Dispensable for Cell Viability in Bacillus subtilis

2006 ◽  
Vol 188 (23) ◽  
pp. 8313-8316 ◽  
Author(s):  
Michael A. D'Elia ◽  
Kathryn E. Millar ◽  
Terry J. Beveridge ◽  
Eric D. Brown
Keyword(s):  
Staphylococcus Aureus ◽  
Cell Wall ◽  
Bacillus Subtilis ◽  
Cell Viability ◽  
Teichoic Acid ◽  
Acid Synthesis ◽  
Extensive Literature ◽  
Wall Teichoic Acid ◽  
First Time ◽  
Similar Gene

ABSTRACT An extensive literature has established that the synthesis of wall teichoic acid in Bacillus subtilis is essential for cell viability. Paradoxically, we have recently shown that wall teichoic acid biogenesis is dispensable in Staphylococcus aureus (M. A. D'Elia, M. P. Pereira, Y. S. Chung, W. Zhao, A. Chau, T. J. Kenney, M. C. Sulavik, T. A. Black, and E. D. Brown, J. Bacteriol. 188:4183-4189, 2006). A complex pattern of teichoic acid gene dispensability was seen in S. aureus where the first gene (tarO) was dispensable and later acting genes showed an indispensable phenotype. Here we show, for the first time, that wall teichoic acid synthesis is also dispensable in B. subtilis and that a similar gene dispensability pattern is seen where later acting enzymes display an essential phenotype, while the gene tagO, whose product catalyzes the first step in the pathway, could be deleted to yield viable mutants devoid of teichoic acid in the cell wall.

scholarly journals The Amino Terminus of Bacillus subtilis TagB Possesses Separable Localization and Functional Properties

2007 ◽  
Vol 189 (19) ◽  
pp. 6816-6823 ◽  
Author(s):  
Amit P. Bhavsar ◽  
Michael A. D'Elia ◽  
Tiffany D. Sahakian ◽  
Eric D. Brown
Keyword(s):  
Bacillus Subtilis ◽  
Protein Function ◽  
Teichoic Acid ◽  
Acid Synthesis ◽  
Amino Terminus ◽  
Membrane Localization ◽  
Membrane Targeting ◽  
Glycerol Phosphate ◽  
Wall Teichoic Acid ◽  
Necessary And Sufficient

ABSTRACT The function(s) of gram-positive wall teichoic acid is emerging with recent findings that it is an important virulence factor in the pathogen Staphylococcus aureus and that it is crucial to proper rod-shaped cell morphology of Bacillus subtilis. Despite its importance, our understanding of teichoic acid biosynthesis remains incomplete. The TagB protein has been implicated in the priming step of poly(glycerol phosphate) wall teichoic acid synthesis in B. subtilis. Work to date indicates that the TagB protein is localized to the membrane, where it adds a single glycerol phosphate residue to the nonreducing end of the undecaprenol-phosphate-linked N-acetylmannosamine-β(1,4)-N-acetylglucosamine-1-phosphate. Thus, membrane association is critical to TagB function. In this work we elucidate the mechanism of TagB membrane localization. We report the identification of a membrane targeting determinant at the amino terminus of TagB that is necessary and sufficient for membrane localization. The putative amphipathicity of this membrane targeting determinant was characterized and shown to be required for TagB function but not localization. This work shows for the first time that the amino terminus of TagB mediates membrane targeting and protein function.

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