scholarly journals The Genetic Analysis of Carbohydrate Utilization in Aspergillus nidulans

1963 ◽  
Vol 31 (1) ◽  
pp. 45-58 ◽  
Author(s):  
C. F. Roberts
Genetics ◽  
1972 ◽  
Vol 72 (4) ◽  
pp. 595-605
Author(s):  
Gordon L Dorn

ABSTRACT A computer system for the storage and processing of microbial meiotic data has been developed. Based on the language Fortran 4, the program retrieves relevant data and determines the order, map distances, and coefficient of coincidence for any three-gene group. Meiotic data from Aspergillus nidulans were used to test the program. A total of 61 three-gene sequences were processed, and the results were found to be compatible with the published values. The advantages and disadvantages of computer analysis for genetic analysis are discussed.


1965 ◽  
Vol 6 (1) ◽  
pp. 13-26 ◽  
Author(s):  
G. Dorn

Summary1. A histochemical method has been applied to the detection of alkaline and acid phosphatase mutants in single colonies of Aspergillus nidulans.2. With the above method it has been possible to isolate mutants in which the alkaline and acid phosphatase activities are affected either separately or simultaneously.3. Crude extracts of wild-type A. nidulans contain four electrophoretically distinct phosphatase components, two with activity at alkaline pH and two with activity at acid pH. Genes affecting three of the four components have been identified.4. Two suppressor mutants of an alkaline phosphataseless mutant (palB7) have been isolated. In a strain carrying palB7 and one of these suppressors, the restoration of an alkaline phosphatase component is accompanied by loss of the faster acid phosphatase component. In a similar strain carrying the other suppressor, the partial restoration of the alkaline phosphatase component goes with an electrophoretic alteration of the slower acid phosphatase component.5. Genetic analysis of twenty-seven mutants has resulted in the identification of fifteen loci affecting the phosphatases. All these loci have been assigned to linkage groups, and twelve of them were also mapped meiotically in relation to other loci.6. One possible model (based on heteropolymeric proteins) has been proposed to account for the electrophoretic and genetic data on the various phosphatase and suppressor mutations.


MedChemComm ◽  
2012 ◽  
Vol 3 (8) ◽  
pp. 997 ◽  
Author(s):  
James F. Sanchez ◽  
Ruth Entwistle ◽  
David Corcoran ◽  
Berl R. Oakley ◽  
Clay C. C. Wang

Genetics ◽  
1990 ◽  
Vol 126 (4) ◽  
pp. 869-874 ◽  
Author(s):  
J L Mooney ◽  
D E Hassett ◽  
L N Yager

Abstract Light-dependent conidiation in the filamentous ascomycete, Aspergillus nidulans, is contingent on the allelic state of the velvet (veA) gene. Light dependence is abolished by a mutation in this gene (veA1), which allows conidiation to occur in the absence of light. We have isolated and characterized six extragenic suppressors of veA1 that restore the light-dependent conidiation phenotype. Alleles of four genes, defined by complementation tests, were subjected to extensive genetic and phenotypic analysis. The results of light-dark shifting experiments and the phenotypes of double mutant combinations are consistent with the possibility that the expression of the light-dependent phenotype is regulated by specific interactions of the suppressor gene products with the velvet gene product and with each other.


2010 ◽  
Vol 6 (3) ◽  
pp. 587-593 ◽  
Author(s):  
James F. Sanchez ◽  
Yi-Ming Chiang ◽  
Edyta Szewczyk ◽  
Ashley D. Davidson ◽  
Manmeet Ahuja ◽  
...  

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