scholarly journals BK polyomavirus genotypes and progression of BKV disease in renal transplant recipients

2020 ◽  
Vol 2 (7A) ◽  
Author(s):  
Nafraa Fallatah
Keyword(s):  
Renal Transplant ◽  
Bk Virus ◽  
Renal Transplant Recipients ◽  
Vp1 Gene ◽  
Transplant Recipients ◽  
Viral Loads ◽  
Acute Graft Rejection ◽  
Bk Polyomavirus ◽  
Genotype Ii ◽  
Acute Graft

Background. BK virus (BKV) reactivation is a significant cause of BK Polyomavirus associated nephropathy (BKPyVN) resulting in acute graft rejection in 1-10% of post-renal transplant recipients. However, the association of BKV genotypes with development of BKPyVN is poorly understood. Here we aimed to determine the prevalence of BKV genotypes in post-renal transplant recipients, and its association with BKPyVN disease progression. Method. Two methods were utilised to genotype BKV. A 800bp fragment of virus VP1 antigen region was amplified using nested (PCR) followed by sequencing. The genotypes were determined according to a previously developed algorithm based on analysing 100bp region of the VP1 gene. Furthermore, the logarithm results were validated with the constructed phylogenetic tree. The results were correlated with patient viral loads and development of BKPyVN. Results. BK virus DNA was detected in 32 (69%) of 46 post-renal transplant recipients with BK viremia, while BKPyVN was only reported in two (4.3%)patients. 30 out of 32 samples were successfully genotyped (93.7%) with 23 (76.6%) belonging to the BKV Ib-2 subtype and seven (23.3%) belonging to the BKV Ib-1 subtype and with no cases representing genotype II, III and IV. All cases with confirmed BKPyVN matched to the BKV Ib-2 genotype. Additionally, no significance differences were observed between BKV genotypes in regards to viral loads, development of viremia, HLA mismatch, age or sex. Conclusion. The results indicate no correlations between BKV genotypes and the development of BKPyVN. Furthermore,a high distribution of BKV genotype Ib-2 was found among BKV infected patients within this cohort.

scholarly journals Viruria of Human BK Virus and John Cunningham Virus among Renal Transplant Recipients and Healthy Control in Southeast of Caspian Sea

Intervirology ◽  
2021 ◽  
pp. 1-8
Author(s):  
Fereshteh Safaei ◽  
Alireza Mohebbi ◽  
Mina Hassanpour ◽  
Hadi Razavi Nikoo ◽  
Alijan Tabarraei
Keyword(s):  
Renal Transplant ◽  
Bk Virus ◽  
Control Group ◽  
Renal Transplant Recipients ◽  
Transplant Recipients ◽  
Genotype I ◽  
The North ◽  
North Of Iran ◽  
Healthy Control ◽  
Genotype Ii

Background: Members of the Polyomaviridae family, BK virus (BKV), and John Cunningham virus (JCV) are linked to polyomavirus-associated nephropathy-associated transplant rejection in immunodeficient patients. Objective: The aim of the study was to evaluate the prevalence of BKV and JCV in immunocompetent individuals in the north of Iran. Methods: Ninety-one urine samples were obtained from renal transplant recipients with a mean age of 39.78 ± 11.19 years. A healthy control group of 65 volunteers with an average age of 40.32 ± 10.7 years also contributed. After DNA extraction, positive cases were detected through PCR. Genotyping was done by alignment and phylogenetic tree construction of the VP1 region against all known JCV and BKV genotypes. Results: The prevalence of BKV and JCV was 15.38 and 19.78%, respectively. JCV was detected in 7.69% of the control group. The prevalence of the BKV between the case and control groups was significant (p < 0.0001). There was no significant association between BKV and JCV and duration of dialysis (p > 0.05). Overall, 62.16% of JCV cases were genotype I. Besides, genotype II was dominant within patients with BKV-positive patients. Discussion: The results obtained here show a relatively lower prevalence of BKV and JCV in immunocompromised renal transplant receivers and healthy control than those reported from other areas in Iran. JCV genotyping was evaluated for the first time in Iran. Genotype I for JCV and genotype II for BKV were dominant genotypes in the north of Iran.

scholarly journals BK Virus-Specific Antibodies and BKV DNA in Renal Transplant Recipients with BKV Nephritis

2005 ◽  
Vol 5 (11) ◽  
pp. 2719-2724 ◽  
Author(s):  
Sundaram Hariharan ◽  
Eric P. Cohen ◽  
Brahm Vasudev ◽  
Rimas Orentas ◽  
Raphael P. Viscidi ◽  
...  
Keyword(s):  
Renal Transplant ◽  
Bk Virus ◽  
Renal Transplant Recipients ◽  
Transplant Recipients ◽  
Specific Antibodies

Human BK Polyomavirus Nephropathy (BKVN) In Non- Kidney-Transplant Patients

2020 ◽  
Vol 154 (Supplement_1) ◽  
pp. S118-S118
Author(s):  
Y Chen Wongworawat ◽  
C Zuppan
Keyword(s):  
Renal Transplant ◽  
Kidney Transplant ◽  
Graft Loss ◽  
Renal Transplant Recipients ◽  
Transplant Recipients ◽  
Transplant Patients ◽  
Bk Polyomavirus ◽  
Pathologic Features ◽  
Renal Biopsies ◽  
Renal Transplant Patients

Abstract Introduction/Objective Human BK polyomavirus nephropathy (BKVN) occurs in up to 10% of renal transplant recipients, and can result in graft loss. Transplant biopsy is the gold standard to diagnose BKVN, and SV40 immunohistochemical (IHC) staining is helpful in confirming the diagnosis. BKVN is uncommon outside the setting of renal transplantation. To understand more about its occurrence in other contexts, we reviewed our renal biopsies files for cases of BKVN. Methods Our renal biopsy files for the past 20 years were reviewed for all cases with a diagnosis of BKVN or polyoma virus infection, and the clinical characteristics of the affected patients noted. Results Evidence of BKVN was found in 44 renal biopsies, of which 39 (86%) were renal transplant patients. Of the remaining five patients (14%), two had undergone heart transplantation, one lung transplantation, one was undergoing chemotherapy for acute lymphoblastic leukemia, and one patient had active HIV infection. All patients had elevated serum creatinine, and four out of five patients had documented BK viremia. Four of the five biopsies showed typical tubular injury with viral nuclear cytopathic changes (inclusions). In the lung transplant patient, the biopsy showed advanced chronic tubulointerstitial injury without distinct viral inclusions, but SV40 staining confirmed the presence of BK virus antigen. Conclusion The BKVN is distinctly uncommon outside the context of kidney transplantation. In our series, 14% of patients with BKVN were not kidney transplant recipients, but all were immune compromised in some fashion. The pathologic features of BKVN appear similar, regardless of whether the host is a renal transplant recipient or not. Although uncommon, it is important to consider the possibility of BKVN in non-renal transplant patients with persistent or progressive renal dysfunction.

MO998EARLY VERSUS LATE ACUTE GRAFT PYELONEPHRITIS: A RETROSPECTIVE ANALYSIS OF GRAFT AND PATIENT OUTCOMES

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
Anupma Kaul ◽  
Thomas Mathews
Keyword(s):  
Renal Transplant ◽  
Graft Survival ◽  
Patient Survival ◽  
Univariate Analysis ◽  
Graft Loss ◽  
Renal Transplant Recipients ◽  
Transplant Recipients ◽  
Kaplan Meier ◽  
Baseline Characteristics ◽  
Acute Graft

Abstract Background and Aims Acute graft pyelonephritis(AGPN) is thought to affect graft and patient survival among renal transplant recipients. Our objective was to compare these outcomes in those having early AGPN(&lt;6 months from transplant) versus those having late AGPN(&gt;6months from transplant) Method This retrospective study analyzed 150 patients who had AGPN over a period of 8 years from 2005 to 2013. They were divided into early AGPN group and late AGPN group. Their baseline characteristics were compared. Predictors of graft loss and mortality were compared using logistic regression analysis. Graft survival and patient survival were analyzed using Kaplan-Meyer survival plots Results A total of 150 patients with AGPN were analyzed. Of these 55.3% (n=83) had early AGPN and 44.7% (n=67) had late AGPN. These two groups were comparable regarding baseline characteristics and immunosuppression. In early AGPN group, 13.3% (n=11) patients had CMV disease during follow up compared to 3% (n=2) in late AGPN group(p&lt;0.05). In the early AGPN group, 26.5% (n=22) had prolonged Foley’s catheterization (&gt;5days) following transplant surgery compared to 7.5% (n=5) in late AGPN group (p&lt;0.05). In the early AGPN group, 38.6% (n=32) had prolonged DJ stent in-situ (&gt;2weeks) following transplant surgery compared to 19.4% (n=13) in the late AGPN group (p&lt;0.05). Recurrent GPN was more common in the late AGPN group than the early AGPN group - 35.8% (n=24) versus 18.1% (n=15). Predictors for graft loss were assessed in patients with AGPN and the presence of renal abscess was predictive of graft loss in univariate analysis (HR-6.129, 95% CI 1.776–21.154, p-0.004). There were no significant predictors of mortality in univariate analysis. Kaplan Meier survival analysis showed decreased death censored graft survival in the early AGPN group (p-0.035). There was no Conclusion Occurrence of early AGPN had a significant impact on long term graft survival in renal transplant recipients with no significant effect on patient survival. This study underlines the paramount importance of the prevention of UTIs in renal transplant recipients.

BK virus replication in renal transplant recipients: Analysis of potential risk factors may contribute in reactivation

2017 ◽  
Vol 96 ◽  
pp. 7-11 ◽  
Author(s):  
Mohammad Shenagari ◽  
Ali Monfared ◽  
Hadise Eghtedari ◽  
Aydin Pourkazemi ◽  
Tolou Hasandokht ◽  
...  
Keyword(s):  
Risk Factors ◽  
Renal Transplant ◽  
Virus Replication ◽  
Potential Risk ◽  
Bk Virus ◽  
Renal Transplant Recipients ◽  
Transplant Recipients ◽  
Potential Risk Factors

Nucleic Acid Tests for BK Polyomavirus Is Critical in Renal Transplant Recipients

2018 ◽  
Vol 50 (8) ◽  
pp. 2489-2492
Author(s):  
Y.-L. Chang ◽  
Y.-T. Cheng ◽  
T.-C. Chen ◽  
Y.-S. Chien ◽  
W.-C. Lee ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Renal Transplant ◽  
Renal Transplant Recipients ◽  
Transplant Recipients ◽  
Bk Polyomavirus ◽  
Nucleic Acid Tests
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