scholarly journals Dethiosulfovibrio salsuginis sp. nov., an anaerobic, slightly halophilic bacterium isolated from a saline spring

2010 ◽  
Vol 60 (4) ◽  
pp. 850-853 ◽  
Author(s):  
C. Díaz-Cárdenas ◽  
G. López ◽  
B. K. C. Patel ◽  
S. Baena
Keyword(s):  
Halophilic Bacterium ◽  
Rrna Gene ◽  
Strictly Anaerobic ◽  
Chromosomal Dna ◽  
Colombian Andes ◽  
Gene Sequence Analysis ◽  
Curved Rods ◽  
Slightly Halophilic Bacterium ◽  
Nitrate And Nitrite ◽  
Saline Spring

A mesophilic, strictly anaerobic, slightly halophilic bacterium, designated strain USBA 82T, was isolated from a terrestrial saline spring in the Colombian Andes. The non-spore-forming curved rods (5–7×1.3 μm) with pointed or rounded ends, stained Gram-negative and were motile by means of laterally inserted flagella. The strain grew optimally at 30 °C (growth range 20–40 °C), pH 7.3 (growth range pH 5.5–8.5) and 2 % (w/v) NaCl (growth range 0.1–7 % NaCl). The strain fermented peptides, amino acids and a few organic acids, but growth was not observed on carbohydrates, alcohols or fatty acids. The strain reduced thiosulfate and sulfur to sulfide. Sulfate, sulfite, nitrate and nitrite were not used as electron acceptors. On peptone alone, acetate, succinate, propionate and traces of ethanol were formed, but in the presence of thiosulfate, acetate and succinate were formed. The G+C content of the chromosomal DNA was 52 mol% (T m).16S rRNA gene sequence analysis indicated that strain USBA 82T was affiliated to Dethiosulfovibrio peptidovorans within the phylum Synergistetes with a similarity value of approximately 93 %. Based on the differences between the new strain and the type species of the genus Dethiosulfovibrio, we suggest that strain USBA 82T represents a novel species of the genus for which the name Dethiosulfovibrio salsuginis sp. nov. is proposed. The type strain is USBA 82T (=DSM 21565T=KCTC 5659T).

scholarly journals Tistlia consotensis gen. nov., sp. nov., an aerobic, chemoheterotrophic, free-living, nitrogen-fixing alphaproteobacterium, isolated from a Colombian saline spring

2010 ◽  
Vol 60 (6) ◽  
pp. 1437-1443 ◽  
Author(s):  
C. Díaz-Cárdenas ◽  
B. K. C. Patel ◽  
S. Baena
Keyword(s):  
Gene Sequence ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Nitrogen Fixing ◽  
Chromosomal Dna ◽  
Colombian Andes ◽  
Gene Sequence Analysis ◽  
Novel Genus And Species ◽  
Curved Rods ◽  
Saline Spring

A Gram-negative, aerobic, mesophilic, non-spore-forming, chemotrophic, chlorophyll-lacking, nitrogen-fixing bacterium, designated strain USBA 355T, was isolated from the saline spring ‘Salado de Consotá’ situated in the Colombian Andes. The non-flagellated cells of strain USBA 355T were straight to slightly curved rods (0.6–0.7 × 3.0–3.5 μm). Growth occurred optimally at 30 °C (growth temperature range between 20 and 40 °C), at pH 6.5–6.7 (pH growth range between 5.0 and 8.0) and at 0.5 % NaCl (w/v) (range between 0 and 4 %). The major quinone present was Q-10 and the predominant fatty acids identified were C19 : 0 cyclo ω8c, C18 : 1 ω7c and C18 : 0. The G+C content of the chromosomal DNA was 71±1 mol%. 16S rRNA gene sequence analysis indicated that strain USBA 355T formed a distant phylogenetic line of descent with members of the genus Thalassobaculum, family Rhodospirillaceae, class Alphaproteobacteria (90 % gene sequence similarity). Comparison of the phylogenetic, chemotaxonomic and physiological features of strain USBA 355T with all other members of the family Rhodospirillaceae suggested that it represents a novel genus and species for which the name Tistlia consotensis gen. nov., sp. nov. is proposed. The type strain of the type species is USBA 355T (=JCM 15529T=KCTC 22406T).

Cyclobacterium halophilum sp. nov., a marine bacterium isolated from a coastal-marine wetland

2014 ◽  
Vol 64 (Pt_3) ◽  
pp. 1000-1005 ◽  
Author(s):  
Azadeh Shahinpei ◽  
Mohammad Ali Amoozegar ◽  
Abbas Akhavan Sepahy ◽  
Peter Schumann ◽  
Antonio Ventosa
Keyword(s):  
Marine Bacterium ◽  
Halophilic Bacterium ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Content Type ◽  
Link Type ◽  
Coastal Marine ◽  
Gene Sequence Analysis ◽  
Lipid Pattern ◽  
Slightly Halophilic Bacterium

A novel Gram-stain-negative, slightly halophilic bacterium, designated strain GASx41T, was isolated from soil of the coastal–marine wetland Gomishan in Iran. Cells of strain GASx41T were curved, ring-like or horseshoe-shaped rods and non-motile. Strain GASx41T was strictly aerobic, and catalase- and oxidase-positive. The strain was able to grow at NaCl concentrations of 1–10 % (w/v), with optimum growth occurring at 2.5–3 % (w/v) NaCl. The optimum temperature and pH for growth were 25–30 °C and pH 7.5–8.0. On the basis of 16S rRNA gene sequence analysis, strain GASx41T was shown to belong to the genus Cyclobacterium within the phylum Bacteroidetes and showed closest phylogenetic similarity to ‘Cyclobacterium jeungdonense’ HMD3055 (98.0 %). The DNA G+C content of strain GASx41T was 48.1 mol%. The major cellular fatty acids of strain GASx41T were iso-C15 : 0, summed feature 4 (iso-C15 : 0 2-OH and/or C16 : 1ω7c), anteiso-C15 : 0 2-OH, anteiso-C15 : 0 and iso-C17 : 0 3-OH, and its polar lipid pattern consisted of phosphatidylethanolamine, phosphatidylcholine and 12 unknown lipids. The only quinone present was menaquinone 7 (MK-7). All these features confirmed the placement of isolate GASx41T within the genus Cyclobacterium . On the basis of evidence from this study, a novel species of the genus Cyclobacterium , Cyclobacterium halophilum sp. nov., is proposed, with strain GASx41T ( = IBRC-M 10761T = CECT 8341T) as the type strain.

scholarly journals Biodegradation of the pyrethroid pesticide cyfluthrin by the halophilic bacterium Photobacterium ganghwense isolated from coral reef ecosystem

2020 ◽  
Vol 67 (4) ◽  
Author(s):  
V.S. Jayasree ◽  
K. S. Sobhana ◽  
Priyanka Poulose ◽  
Keerthi R. Babu ◽  
S. Jasmine ◽  
...  
Keyword(s):  
Morphological Changes ◽  
Halophilic Bacterium ◽  
Culture Conditions ◽  
Mineral Medium ◽  
Fish Cell ◽  
Rrna Gene ◽  
Gene Sequence Analysis ◽  
Pyrethroid Pesticide ◽  
Ph Conditions ◽  
Epinephelus Malabaricus

A halophilic bacterial strain T14 isolated from the mucus of coral Acropora sp. was found to be highly effective in degrading the pyrethroid pesticide, cyfluthrin. T14 was identified as Photobacterium ganghwense (GenBank Accession No. MT360254) based on phenotypic and biochemical characteristics as well as by 16S rRNA gene sequence analysis. The pyrethroid degrading efficiency of P. ganghwense T14 strain was examined under different culture conditions. It was observed that P. ganghwense T14 was able to utilise cyfluthrin as a sole carbon source and was found to grow on mineral medium with pesticide concentrations ranging from 10 to 100 mg l-1. Optimal temperature and pH conditions for efficient cyfluthrin degradation by P. ganghwense T14 were determined as 30° C and 8 respectively. Degradation of cyfluthrin by P. ganghwense T14 was quantitated by gas chromatography-tandem mass spectrometry (GC-MS/MS). Mineral medium supplemented with 100 mg l-1 cyfluthrin and inoculated with P. ganghwense T14 (106 cells ml-1) recorded 92.13% pesticide decomposition within 120 h. Cytotoxicity assay on a fish cell line EM4SpEx derived from the grouper Epinephelus malabaricus, revealed a drastic reduction in cyfluthrin toxicity as evidenced by reduction in the intensity of cell destruction as well as morphological changes when exposed to P. ganghwense T14 treated filtrate, in comparison with that of parent cyfluthrin filtrate. Results of the study clearly indicated potential bioremediative use of P. ganghwense T14 in cyfluthrin contaminated sites.

scholarly journals Methanobacterium aggregans sp. nov., a hydrogenotrophic methanogenic archaeon isolated from an anaerobic digester

2015 ◽  
Vol 65 (Pt_6) ◽  
pp. 1975-1980 ◽  
Author(s):  
Tobias Kern ◽  
Mary Linge ◽  
Michael Rother
Keyword(s):  
16S Rrna ◽  
Novel Species ◽  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Optimal Growth ◽  
Maximum Growth ◽  
Maximum Growth Rate ◽  
Rrna Gene ◽  
Strictly Anaerobic ◽  
Curved Rods

A novel, strictly anaerobic, hydrogenotrophic methanogen, strain E09F.3T, was isolated from a commercial biogas plant in Germany. Cells of E09F.3T were Gram-stain-negative, non-motile, slightly curved rods, long chains of which formed large aggregates consisting of intertwined bundles of chains. Cells utilized H2+CO2 and, to a lesser extent, formate as substrates for growth and methanogenesis. The optimal growth temperature was around 40 °C; maximum growth rate was obtained at pH around 7.0 with approximately 6.8 mM NaCl. The DNA G+C content of strain E09F.3T was 39.1 mol%. Phylogenetic analyses based on 16S rRNA and mcrA gene sequences placed strain E09F.3T within the genus Methanobacterium. On the basis of 16S rRNA gene sequence similarity, strain E09F.3T was closely related to Methanobacterium congolense CT but morphological, physiological and genomic characteristics indicated that strain E09F.3T represents a novel species. The name Methanobacterium aggregans sp. nov. is proposed for this novel species, with strain E09F.3T ( = DSM 29428T = JCM 30569T) as the type strain.

scholarly journals Cobetia crustatorum sp. nov., a novel slightly halophilic bacterium isolated from traditional fermented seafood in Korea

2010 ◽  
Vol 60 (3) ◽  
pp. 620-626 ◽  
Author(s):  
Min-Soo Kim ◽  
Seong Woon Roh ◽  
Jin-Woo Bae
Keyword(s):  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Nitrogen Sources ◽  
Optimal Growth ◽  
Halophilic Bacterium ◽  
Rrna Gene ◽  
Carbon And Nitrogen ◽  
Cobetia Marina ◽  
Slightly Halophilic Bacterium ◽  
Straight Rod

A slightly halophilic, Gram-stain-negative, straight-rod-shaped aerobe, strain JO1T, was isolated from jeotgal, a traditional Korean fermented seafood. Cells were observed singly or in pairs and had 2–5 peritrichous flagella. Optimal growth occurred at 25 °C, in 6.5 % (w/v) salts and at pH 5.0–6.0. Strain JO1T was oxidase-negative and catalase-positive. Cells did not reduce fumarate, nitrate or nitrite on respiration. Acid was produced from several carbohydrates and the strain utilized many sugars and amino acids as carbon and nitrogen sources. The main fatty acids were C12 : 0 3-OH, C16 : 0, C17 : 0 cyclo and summed feature 3 (C16 : 1 ω7c/iso-C15 : 0 2-OH). DNA–DNA hybridization experiments with strain JO1T and Cobetia marina DSM 4741T revealed 24 % relatedness, although high 16S rRNA gene sequence similarity (98.9 %) was observed between these strains. Based on phenotypic, genotypic and phylogenetic analyses, it is proposed that the isolate from jeotgal should be classified as a representative of a novel species, Cobetia crustatorum sp. nov., with strain JO1T (=KCTC 22486T=JCM 15644T) as the type strain.

scholarly journals Acetobacteroides hydrogenigenes gen. nov., sp. nov., an anaerobic hydrogen-producing bacterium in the family Rikenellaceae isolated from a reed swamp

2014 ◽  
Vol 64 (Pt_9) ◽  
pp. 2986-2991 ◽  
Author(s):  
Xiao-Li Su ◽  
Qi Tian ◽  
Jie Zhang ◽  
Xian-Zheng Yuan ◽  
Xiao-Shuang Shi ◽  
...  
Keyword(s):  
Yeast Extract ◽  
Type Species ◽  
Wastewater Sludge ◽  
Rrna Gene ◽  
Strictly Anaerobic ◽  
Content Type ◽  
Link Type ◽  
Gene Sequence Analysis ◽  
The Family ◽  
Sequence Similarities

A strictly anaerobic, mesophilic, carbohydrate-fermenting, hydrogen-producing bacterium, designated strain RL-CT, was isolated from a reed swamp in China. Cells were Gram-stain-negative, catalase-negative, non-spore-forming, non-motile rods measuring 0.7–1.0 µm in width and 3.0–8.0 µm in length. The optimum temperature for growth of strain RL-CT was 37 °C (range 25–40 °C) and pH 7.0–7.5 (range pH 5.7–8.0). The strain could grow fermentatively on yeast extract, tryptone, arabinose, glucose, galactose, mannose, maltose, lactose, glycogen, pectin and starch. The main end products of glucose fermentation were acetate, H2 and CO2. Organic acids, alcohols and amino acids were not utilized for growth. Yeast extract was not required for growth; however, it stimulated growth slightly. Nitrate, sulfate, sulfite, thiosulfate, elemental sulfur and Fe(III) nitrilotriacetate were not reduced as terminal electron acceptors. Aesculin was hydrolysed but not gelatin. Indole and H2S were produced from yeast extract. The G+C content of the genomic DNA was 51.2 mol%. The major cellular fatty acids were iso-C15 : 0, anteiso-C15 : 0 and C16 : 0. The most abundant polar lipid of strain RL-CT was phosphatidylethanolamine. 16S rRNA gene sequence analysis revealed that the isolate belongs to the uncultured Blvii28 wastewater-sludge group (http://www.arb-silva.de/) in the family Rikenellaceae of the phylum Bacteroidetes, and shared low sequence similarities with the related species Alistipes shahii WAL 8301T (81.8 %), Rikenella microfusus ATCC 29728T (81.7 %) and Anaerocella delicata WN081T (80.9 %). On the basis of these data, a novel species in a new genus of the family Rikenellaceae is proposed, Acetobacteroides hydrogenigenes gen. nov., sp. nov. The type strain of the type species is RL-CT ( = JCM 17603T = DSM 24657T = CGMCC 1.5173T).

scholarly journals Bacillus halochares sp. nov., a halophilic bacterium isolated from a solar saltern

2010 ◽  
Vol 60 (6) ◽  
pp. 1432-1436 ◽  
Author(s):  
A. Pappa ◽  
C. Sánchez-Porro ◽  
P. Lazoura ◽  
A. Kallimanis ◽  
A. Perisynakis ◽  
...  
Keyword(s):  
Gene Sequence ◽  
Novel Species ◽  
Optimal Growth ◽  
Halophilic Bacterium ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Gram Stain ◽  
Rrna Gene Sequence ◽  
Gene Sequence Analysis ◽  
Lipid Pattern

A novel halophilic bacterium, designated strain MSS4T, was isolated from the solar salterns of Mesolongi, Greece. The micro-organism, a motile, Gram-stain-positive, aerobic rod, proliferated at salinities of 1.0–4.0 M NaCl, with optimal growth at 2.5 M NaCl. Endospores were not observed. Strain MSS4T showed optimal growth at 37 °C and pH 8.0. The G+C content of its DNA was 47.2 mol%. The polar lipid pattern of strain MSS4T consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidic acid and phosphatidylethanolamine. It possessed anteiso-C15 : 0, C18 : 0, C16 : 0 and anteiso-C17 : 0 as the major fatty acids (altogether representing 84.7 % of the total). The predominant isoprenoid quinone was MK-7. The cell-wall peptidoglycan contained meso-diaminopimelic acid. 16S rRNA gene sequence analysis showed that the new isolate has 96.1 % similarity to Bacillus qingdaonensis CM1T and Bacillus aidingensis 17-5T, 95.5 % to Bacillus salarius BH169T and lower similarity to other Bacillus species. These results justify the assignment of strain MSS4T to a novel species within the genus Bacillus, for which the name Bacillus halochares sp. nov. is proposed. The type strain is MSS4T (=LMG 24571T =DSM 21373T).

scholarly journals Clostridium arbusti sp. nov., an anaerobic bacterium isolated from pear orchard soil

2010 ◽  
Vol 60 (9) ◽  
pp. 2231-2235 ◽  
Author(s):  
Min Young Jung ◽  
In-Soon Park ◽  
Wonyong Kim ◽  
Hong Lim Kim ◽  
Woon Kee Paek ◽  
...  
Keyword(s):  
Acid Production ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Gene Sequence Analysis ◽  
Orchard Soil ◽  
Pear Orchard ◽  
Curved Rods ◽  
Unique Species ◽  
Orchard Soils ◽  
Acetate Butyrate

An obligately anaerobic, Gram-positive, spore-forming bacterial strain, designated SL206T, was isolated from pear orchard soils. Strain SL206T cells were straight or slightly curved rods, with motility by peritrichate flagella. Cell walls contained meso-diaminopimelic acid; wall sugars were glucose, rhamnose and mannose. The major fatty acids were C16 : 0, C18 : 1 ω9c and summed feature 10 (containing C18 : 1 ω11c/9t/6t). API 20A reactions were negative for oxidase, catalase and acid production from l-rhamnose, sucrose, trehalose, d-xylose, melezitose, salicin and d-sorbitol, and positive for acid production from d-glucose, sucrose, maltose, d-mannose and raffinose. Glucose was fermented to acetate, butyrate, CO2, H2 and ethanol in culture. The G+C content of the genomic DNA was 31.1 mol%. Based on comparative 16S rRNA gene sequence analysis, the isolate belonged to the genus Clostridium and formed a clade with Clostridium pasteurianum. The species most closely related to strain SL206T were C. pasteurianum (98.6 % similarity) and Clostridium acidisoli (97.8 % similarity). In DNA–DNA relatedness studies, the isolate had 59.5 % relatedness with C. pasteurianum and thus represented a unique species. On the basis of these studies, strain SL206T (=KCTC 5449T =JCM 14858T) is proposed to represent the type strain of a novel species, Clostridium arbusti sp. nov.

scholarly journals Desulfosoma caldarium gen. nov., sp. nov., a thermophilic sulfate-reducing bacterium from a terrestrial hot spring

2011 ◽  
Vol 61 (4) ◽  
pp. 732-736 ◽  
Author(s):  
Sandra Baena ◽  
Natalia Perdomo ◽  
Catalina Carvajal ◽  
Carolina Díaz ◽  
Bharat K. C. Patel
Keyword(s):  
Gene Sequence ◽  
Hot Spring ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Terminal Electron Acceptor ◽  
Sulfate Reducing ◽  
Colombian Andes ◽  
Gene Sequence Analysis ◽  
Lactate Degradation ◽  
Terrestrial Hot Spring

A thermophilic, sulfate-reducing bacterium, designated strain USBA-053T, was isolated from a terrestrial hot spring located at a height of 2500 m in the Colombian Andes (5° 45′ 33.29″ N 73° 6′ 49.89″ W), Colombia. Cells of strain USBA-053T were oval- to rod-shaped, Gram-negative and motile by means of a single polar flagellum. The strain grew autotrophically with H2 as the electron donor and heterotrophically on formate, propionate, butyrate, valerate, isovalerate, lactate, pyruvate, ethanol, glycerol, serine and hexadecanoic acid in the presence of sulfate as the terminal electron acceptor. The main end products from lactate degradation, in the presence of sulfate, were acetate, CO2 and H2S. Strain USBA-053T fermented pyruvate in the absence of sulfate and grew optimally at 57 °C (growth temperature ranged from 50 °C to 62 °C) and pH 6.8 (growth pH ranged from 5.7 to 7.7). The novel strain was slightly halophilic and grew in NaCl concentrations ranging from 5 to 30 g l−1, with an optimum at 25 g l−1 NaCl. Sulfate, thiosulfate and sulfite were used as electron acceptors, but not elemental sulfur, nitrate or nitrite. The G+C content of the genomic DNA was 56±1 mol%. 16S rRNA gene sequence analysis indicated that strain USBA-053T was a member of the class Deltaproteobacteria, with Desulfacinum hydrothermale MT-96T as the closest relative (93 % gene sequence similarity). On the basis of physiological characteristics and phylogenetic analysis, it is suggested that strain USBA-053T represents a new genus and novel species for which the name Desulfosoma caldarium gen. nov., sp. nov. is proposed. The type strain of the type species is USBA-053T ( = KCTC 5670T = DSM 22027T).

Fonticella tunisiensis gen. nov., sp. nov., isolated from a hot spring

2013 ◽  
Vol 63 (Pt_6) ◽  
pp. 1947-1950 ◽  
Author(s):  
Belkis Fraj ◽  
Wajdi Ben Hania ◽  
Anne Postec ◽  
Moktar Hamdi ◽  
Bernard Ollivier ◽  
...  
Keyword(s):  
Type Species ◽  
Hot Spring ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Strictly Anaerobic ◽  
Fermentation Products ◽  
Content Type ◽  
Moderately Thermophilic ◽  
Link Type ◽  
Nitrate And Nitrite

A strictly anaerobic, moderately thermophilic, halotolerant rod, designated BELH25T, was isolated from a water sample of a Tunisian hot spring. Cells were non-motile, 2–6 µm long and 0.4–0.6 µm wide, appearing singly or in pairs. The isolate grew at 45–70 °C (optimum 55 °C), at pH 6.2–8.0 (optimum pH 7.0) and with 0–4 % NaCl (optimum 0–2.0 %). Sulfate, thiosulfate, elemental sulfur, sulfite, nitrate and nitrite were not used as terminal electron acceptors. Strain BELH25T used cellobiose, fructose, galactose, glucose, maltose, mannose, sucrose, starch and yeast extract as electron donors. The main fermentation products from glucose metabolism were formate, acetate, ethanol and CO2. The predominant cellular fatty acids were iso-C15 : 0, iso-C17 : 0 and anteiso-C15 : 0. The DNA G+C content was 37.2 mol%. Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain BELH25T was most closely related to Caloramator viterbiensis JW/MS-VS5T and Fervidicella metallireducens AeBT (92.2 and 92.1 % sequence similarity, respectively), and the isolate was positioned approximately equidistantly between these genera. Based on phenotypic, phylogenetic and chemotaxonomic characteristics, strain BELH25T is proposed to be a member of a novel species of a novel genus within the order Clostridiales , family Clostridiaceae , for which the name Fonticella tunisiensis gen. nov., sp. nov. is proposed. The type strain of the type species is BELH25T ( = DSM 24455T = JCM 17559T).

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