scholarly journals Sphingomonas changbaiensis sp. nov., isolated from forest soil

2010 ◽  
Vol 60 (4) ◽  
pp. 790-795 ◽  
Author(s):  
Jia-Yue Zhang ◽  
Xing-Yu Liu ◽  
Shuang-Jiang Liu
Keyword(s):  
Forest Soil ◽  
Novel Species ◽  
Changbai Mountains ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
16S Rrna Gene Sequences ◽  
Respiratory Quinone ◽  
Optimum Ph ◽  
Major Respiratory Quinone ◽  
Sequence Similarities

Bacterial strain V2M44T was isolated from forest soil from the Changbai Mountains, Heilongjiang Province, China. Cells of strain V2M44T were Gram-negative, rod-shaped and 0.3–0.4 μm in diameter and 1.5–2.5 μm long. The cells were strictly aerobic and were motile by means of peritrichous flagella. Growth occurred at 20–33 °C (optimum, 29–31 °C), at pH 5–8 (optimum, pH 6.0–7.0) and in the presence of 0−0.1 % (w/v) NaCl (optimum, 0.05 %). Strain V2M44T contained C17 : 1 ω6c (38.9 %) and summed feature 7 (comprising C18 : 1 ω7c, C18 : 1 ω9t and/or C18 : 1 ω12t, 31.5 %) as the major cellular fatty acids and Q-10 as the major respiratory quinone. The major polar lipids were sphingoglycolipid, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol along with two unidentified phospholipids and four unidentified glycolipids. The major component in the polyamine pattern was sym-homospermidine. The DNA G+C content was 65.8 mol%. Analysis of 16S rRNA gene sequences indicated that strain V2M44T was phylogenetically related to members of the genus Sphingomonas, with sequence similarities ranging from 92.0–95.8 %. Based on these results, it is concluded that strain V2M44T represents a novel species of the genus Sphingomonas, for which the name Sphingomonas changbaiensis sp. nov. is proposed. The type strain is V2M44T (=CGMCC 1.7057T=NBRC 104936T).

Phycicoccus cremeus sp. nov., isolated from forest soil, and emended description of the genus Phycicoccus

2011 ◽  
Vol 61 (1) ◽  
pp. 71-75 ◽  
Author(s):  
Jia-Yue Zhang ◽  
Xing-Yu Liu ◽  
Shuang-Jiang Liu
Keyword(s):  
Forest Soil ◽  
Diaminopimelic Acid ◽  
Changbai Mountains ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Respiratory Quinone ◽  
Gene Sequence Analysis ◽  
Emended Description ◽  
Major Respiratory Quinone ◽  
Sequence Similarities

A bacterial strain, designated V2M29T, was isolated from forest soil collected in the Changbai Mountains, Heilongjiang Province, China. Cells of strain V2M29T were Gram-positive, rod-shaped (0.5–0.8 μm in diameter and 1.5–2.0 μm in length), strictly aerobic and non-motile. Colonies produced on R2A agar plates were creamy, smooth, circular and 0.3–0.8 mm in diameter. Strain V2M29T grew at 14–35 °C (optimum, 29 °C), at pH 4.1–10.0 (optimum, pH 7.0–8.0) and in the presence of 0–7.0 % (w/v) NaCl (optimum, 0–2.0 %). Strain V2M29T contained MK-8(H4) as the major respiratory quinone and iso-C15 : 0 (20.9 %), iso-C16 : 0 (18.5 %), C17 : 1 ω8c (16.7 %) and 10-methyl-C17 : 0 (9.2 %) as the major cellular fatty acids. The cell-wall peptidoglycan type was based on meso-2,6-diaminopimelic acid and the acyl type of the muramic acid was acetyl. Diphosphatidylglycerol, phosphatidylinositol and six unknown glycolipids were the major polar lipids. The G+C content of the DNA was 72.0 mol% (T m). 16S rRNA gene sequence analysis indicated that strain V2M29T was phylogenetically related to members of the genus Phycicoccus, with sequence similarities ranging from 96.6–97.8 %. The DNA–DNA relatedness values of strain V2M29T to Phycicoccus dokdonensis DS-8T and Phycicoccus bigeumensis DSM 19264T were 32.5±3.3 % and 29.2±2.3 %, respectively. Based on these results, it is concluded that strain V2M29T represents a novel species of the genus Phycicoccus, for which the name Phycicoccus cremeus sp. nov. is proposed, with V2M29T (=CGMCC 1.6963T =NBRC 104261T) as the type strain.

scholarly journals Algoriphagus jejuensis sp. nov., isolated from seawater

2012 ◽  
Vol 62 (2) ◽  
pp. 409-413 ◽  
Author(s):  
Dong-Heon Lee ◽  
Hyung-Yeel Kahng ◽  
Sun Bok Lee
Keyword(s):  
Novel Species ◽  
Sequence Similarity ◽  
Polar Lipids ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
16S Rrna Gene Sequences ◽  
High Sequence Similarity ◽  
Respiratory Quinone ◽  
Distinct Lineage ◽  
Major Respiratory Quinone

A Gram-negative, pink-pigmented, non-motile, strictly aerobic rod, designated CNU040T, was isolated from seawater from the coast of Jeju Island in Korea. The temperature, pH and NaCl ranges for growth were 4–30 °C, pH 5.5–10.0 and 0–5.0 % NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CNU040T belonged to a distinct lineage in the genus Algoriphagus and exhibited high sequence similarity with Algoriphagus terrigena DS-44T (98.3 %) and Algoriphagus alkaliphilus AC-74T (96.6 %) and lower sequence similarity (<96.0 %) with all other members of the genus Algoriphagus. DNA–DNA relatedness between strain CNU040T and A. terrigena KCTC 12545T was 44.5 %. The DNA G+C content of the isolate was 48.5 mol% and the major respiratory quinone was menaquinone-7. The major cellular fatty acids were iso-C15 : 0 (28.6 %) and summed feature 3 (consisting of iso-C15 : 0 2-OH and/or C16 : 1ω7c; 24.0 %). The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, one unknown amino lipid, one unknown aminophospholipid and three unknown polar lipids. On the basis of phenotypic, phylogenetic and genotypic data, strain CNU040T represents a novel species within the genus Algoriphagus, for which the name Algoriphagus jejuensis sp. nov. is proposed. The type strain is CNU040T ( = KCTC 22647T  = JCM 16112T).

scholarly journals Frateuria terrea sp. nov., isolated from forest soil, and emended description of the genus Frateuria

2011 ◽  
Vol 61 (2) ◽  
pp. 443-447 ◽  
Author(s):  
Jia-Yue Zhang ◽  
Xing-Yu Liu ◽  
Shuang-Jiang Liu
Keyword(s):  
Forest Soil ◽  
Novel Species ◽  
Sequence Similarity ◽  
Changbai Mountains ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Respiratory Quinone ◽  
Cellular Fatty Acids ◽  
Emended Description ◽  
Negative Growth

A bacterial strain, designated VA24T, was isolated from forest soil of the Changbai Mountains, Heilongjiang province, China. Cells of strain VA24T were Gram-reaction-negative, aerobic, non-spore-forming, long rods, 0.3–0.5×2.0–3.0 μm in size and were motile by means of a subpolar flagellum. Strain VA24T was oxidase-positive and catalase-negative. Growth occurred at 21–36 °C, pH 5–10 and in 0–2 % (w/v) NaCl but did not occur at 37 °C. The predominant respiratory quinone was Q-8, the major polar lipids were phosphatidylethanolamine and phosphatidylmonomethylethanolamine and the major cellular fatty acids were iso-C15 : 0 (14.9 %), iso-C17 : 1 ω9c (14.1 %), iso-C17 : 0 (10.8 %) and iso-C16 : 0 (10.3 %). The DNA G+C content was 67.4 mol% (T m). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain VA24T was closely related to Frateuria aurantia IFO 13300T with 96.9 % sequence similarity. DNA–DNA relatedness of strain VA24T to F. aurantia DSM 6220T was 15.8 %. Based on its phenotypic and genotypic features, strain VA24T represents a novel species of the genus Frateuria, for which the name Frateuria terrea sp. nov. is proposed. The type strain is VA24T (=CGMCC 1.7053T =NBRC 104236T).

scholarly journals Youhaiella tibetensis gen. nov., sp. nov., isolated from subsurface sediment

2015 ◽  
Vol 65 (Pt_7) ◽  
pp. 2048-2055 ◽  
Author(s):  
Yun-xiang Wang ◽  
Fa-qi Huang ◽  
Yuichi Nogi ◽  
Shou-ji Pang ◽  
Ping-kang Wang ◽  
...  
Keyword(s):  
New Genus ◽  
Novel Species ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Respiratory Quinone ◽  
Subsurface Sediment ◽  
Qiangtang Basin ◽  
The Family ◽  
Major Respiratory Quinone ◽  
Sequence Similarities

A Gram-reaction-negative bacterial strain, designated fig4T, was isolated from a subsurface sediment core of Qiangtang Basin permafrost in China. Cells were catalase- and oxidase-positive and rods. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain fig4T was a member of the family Hyphomicrobiaceae and was most closely related to members of the genera Pelagibacterium, Vasilyevaea and Devosia with 93.8–96.2 % sequence similarities. The major cellular fatty acids were C16 : 0, C18 : 0, 11-methyl C18 : 1ω7c, C19 : 0 cyclo ω8c and summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c). The major respiratory quinone was Q-10 and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and two unknown glycolipids. The DNA G+C content was 60.7 mol%. Based on the phenotypic, phylogenetic and genotypic data, strain fig4T is considered to represent a novel species of a new genus in the family Hyphomicrobiaceae, for which the name Youhaiella tibetensis gen. nov., sp. nov. is proposed. The type strain is fig4T ( = CGMCC 1.12719T = JCM 19854T).

scholarly journals Chryseobacterium vietnamense sp. nov., isolated from forest soil

2012 ◽  
Vol 62 (Pt_4) ◽  
pp. 827-831 ◽  
Author(s):  
Zhikun Li ◽  
Honghui Zhu
Keyword(s):  
Forest Soil ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Properties ◽  
Respiratory Quinone ◽  
Link Type ◽  
Distinct Lineage

A Gram-reaction-negative bacterial strain, designated GIMN1.005T, was isolated from a forest soil sample in Vietnam. The isolate was yellow-pigmented, strictly aerobic, and unable to grow below 5 °C or above 37 °C and in the presence of more than 2.0 % NaCl. Cells were non-motile, non-gliding and non-spore-forming. The yellow pigment was of the flexirubin type, non-diffusible and non-fluorescent. Analysis of 16S rRNA gene sequences showed that strain GIMN1.005T occupied a distinct lineage within the genus Chryseobacterium , with sequence similarity values of 98.6, 98.5 and 98.3 % to Chryseobacterium aquifrigidense KCTC 12894T, Chryseobacterium jejuense KACC 12501T and Chryseobacterium indologenes GIFU 1347T, respectively. The level of DNA–DNA relatedness between strain GIMN1.005T and C. jejuense KACC 12501T was <30 %. The DNA G+C content of strain GIMN1.005T was 42.1 mol%. The predominant cellular fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c); menaquinone 6 (MK-6) was the sole respiratory quinone. On the basis of phenotypic properties and phylogenetic distinctiveness, strain GIMN1.005T is considered to represent a novel species of the genus Chryseobacterium , for which the name Chryseobacterium vietnamense sp. nov. is proposed. The type strain is GIMN1.005T ( = CCTCC M 209230T = NRRL B-59550T).

scholarly journals Litorimicrobium taeanense gen. nov., sp. nov., isolated from a sandy beach

2011 ◽  
Vol 61 (6) ◽  
pp. 1392-1396 ◽  
Author(s):  
Hyun Mi Jin ◽  
Hyo Jung Lee ◽  
Jeong Myeong Kim ◽  
Moon Su Park ◽  
Kangseok Lee ◽  
...  
Keyword(s):  
Sandy Beach ◽  
Novel Species ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
16S Rrna Gene Sequences ◽  
Unidentified Phospholipid ◽  
The Family ◽  
Positive Growth ◽  
Optimum Ph ◽  
Sequence Similarities

A Gram-reaction-negative, strictly aerobic, non-motile bacterium, designated strain G4T, was isolated from a sandy beach of Taean in South Korea. Cells were ovoid rods and were catalase- and oxidase-positive. Growth of strain G4T was determined at 15–35 °C (optimum 25–30 °C) and pH 6–8 (optimum pH 6.5–7.5). Strain G4T contained Q-10 as the predominant isoprenoid quinone and C18 : 1ω7c (59.0 %), C18 : 1 ω7c 11-methyl (11.3 %) and C12 : 1 3-OH (9.8 %) as the major fatty acids. The major cellular polar lipids were identified as phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine, an unidentified amino lipid, an unidentified phospholipid and an unidentified lipid. The DNA G+C content was 62.4 mol%. Phylogenetic and comparative analysis based on 16S rRNA gene sequences indicated that strain G4T fell within the family Rhodobacteraceae of Alphaproteobacteria and was most closely related to members of the genera Marinovum, Leisingera and Phaeobacter with 95.5–96.4 % sequence similarities. On the basis of phenotypic, chemotaxonomic and molecular properties, strain G4T represents a novel species of a novel genus within the family Rhodobacteraceae, for which the name Litorimicrobium taeanense gen. nov., sp. nov. is proposed. The type strain is G4T ( = KACC 13706T  = DSM 22007T).

scholarly journals Muriicola jejuensis gen. nov., sp. nov., isolated from seawater

2010 ◽  
Vol 60 (7) ◽  
pp. 1644-1648 ◽  
Author(s):  
Hyung-Yeel Kahng ◽  
Sang-Suk Lee ◽  
Jeong Myeong Kim ◽  
Ji Young Jung ◽  
Mee Young Lee ◽  
...  
Keyword(s):  
Eastern Coast ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Respiratory Quinone ◽  
Gram Staining ◽  
The Family ◽  
Optimum Ph ◽  
Yellow Orange ◽  
Major Respiratory Quinone ◽  
Sequence Similarities

An aerobic, yellow–orange-pigmented, Gram-staining-negative bacterium, designated strain EM44T, was isolated from seawater on the eastern coast of Jeju Island, Korea. Growth was observed at 15–35 °C (optimum 25–30 °C), pH 6.5–9.0 (optimum pH 7.0–8.5) and between 1 and 5 % NaCl (w/v) (optimum 2–4 %). Cells of strain EM44T were non-motile, straight rods and showed catalase and oxidase activities. The G+C content of the genomic DNA was 47.9 mol% and the major respiratory quinone was MK-6. The major fatty acids were iso-C15 : 0 G, iso-C15 : 0, iso-C17 : 0 3-OH and iso-C16 : 0 3-OH. Strain EM44T contained phosphatidylethanolamine as a major polar phospholipid. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain EM44T fell within the family Flavobacteriaceae in the phylum Bacteroidetes and was most closely related to members of the genera Eudoraea, Zeaxanthinibacter and Robiginitalea with 92–94.5 % gene sequence similarities. On the basis of chemotaxonomic data and molecular properties, it is clear that strain EM44T represents a novel genus within the family Flavobacteriaceae, for which the name Muriicola jejuensis gen. nov., sp. nov. is proposed. The type strain of the type species is EM44T (=KCTC 22299T=DSM 21206T).

scholarly journals Cesiribacter roseus sp. nov., a pink-pigmented bacterium isolated from desert sand

2012 ◽  
Vol 62 (1) ◽  
pp. 96-99 ◽  
Author(s):  
Ming Liu ◽  
Huan Qi ◽  
Xuesong Luo ◽  
Jun Dai ◽  
Fang Peng ◽  
...  
Keyword(s):  
Novel Species ◽  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Aerobic Bacterium ◽  
16S Rrna Gene Sequences ◽  
Respiratory Quinone ◽  
Cellular Fatty Acids ◽  
Desert Sand ◽  
Major Respiratory Quinone

A pink-pigmented, Gram-negative, rod-shaped, motile, strictly aerobic bacterium, designated strain 311T, was isolated from desert sand in Xinjiang, China. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain 311T was related closely to Cesiribacter andamanensis AMV16T (94.6 % similarity). The DNA G+C content of strain 311T was 47.1 mol% and the major respiratory quinone was menaquinone 7 (MK-7). The main cellular fatty acids were C16 : 1ω5c (29.9 %), iso-C15 : 0 (21.9 %), iso-C17 : 0 3-OH (13.3 %) and summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B; 13.0 %). Based on phenotypic and chemotaxonomic data and phylogenetic analysis, strain 311T is considered to represent a novel species of the genus Cesiribacter, for which the name Cesiribacter roseus sp. nov. is proposed. The type strain is 311T ( = CCTCC AB 207142T  = KACC 15456T).

Idiomarina indica sp. nov., isolated from seawater

2013 ◽  
Vol 63 (Pt_7) ◽  
pp. 2497-2500 ◽  
Author(s):  
Lei Song ◽  
Fei Ren ◽  
Ying Huang ◽  
Xin Dai ◽  
Yuguang Zhou
Keyword(s):  
Type Species ◽  
Novel Species ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Respiratory Quinone ◽  
Content Type ◽  
Link Type ◽  
Optimum Ph ◽  
Major Respiratory Quinone ◽  
The Indian Ocean

Two Gram-negative, aerobic, rod-shaped bacteria, designated strains SW104T and X07, were isolated from a seawater sample collected from the Indian Ocean. The strains grew at a temperature range of 12–50 °C (optimum, 35–37 °C), and at pH 6.0–9.0 (optimum pH 7.0–7.5). The predominant cellular fatty acids of strain SW104T were iso-C15 : 0 (41.2 %), iso-C17 : 1ω9c (15.2 %) and iso-C17 : 0 (11.1 %). The major respiratory quinone was ubiquinone 8 (Q-8). The major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. The genomic DNA G+C contents of strains SW104T and X07 were 49.8 and 49.5 mol%, respectively. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the new isolates were related to members of the genus Idiomarina , showing the highest similarity with Idiomarina taiwanensis PIT1T and Idiomarina maritima 908087T (96.1 and 95.9 %, respectively). On the basis of phenotypic, genotypic and phylogenetic characteristics, it is proposed that strains SW104T and X07 should be described as representatives of a novel species of the genus Idiomarina , for which the name Idiomarina indica sp. nov. is proposed. The type strain is SW104T ( = CGMCC 1.10824T = JCM 18138T).

scholarly journals Flavobacterium procerum sp. nov., isolated from freshwater

2015 ◽  
Vol 65 (Pt_8) ◽  
pp. 2702-2708 ◽  
Author(s):  
Qingqing Feng ◽  
Lu Han ◽  
Xin Yuan ◽  
Xu Tan ◽  
Yuan Gao ◽  
...  
Keyword(s):  
Gene Sequence ◽  
Novel Species ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
The Novel ◽  
Respiratory Quinone ◽  
West China ◽  
Optimum Ph ◽  
Chishui River ◽  
Major Respiratory Quinone

A Gram-reaction-negative, strictly aerobic, yellow-pigmented, rod-shaped bacterium, designated strain T3T, was isolated from freshwater of Chishui River flowing through Maotai town, Guizhou, south-west China. Analysis of the16S rRNA gene sequence indicated that strain T3T was a member of the genus Flavobacterium and closely related to Flavobacterium resistens DSM 19382T (96.8  %). The novel strain was able to grow at 10–34 °C (optimum 28 °C), pH 6.0–11.0 (optimum pH 8.0–9.0) and with 0–2.0  % (w/v) NaCl (optimum 0  %). The major polar lipids were phosphatidylethanolamine, two unknown glycolipids, five unknown aminolipids and four unidentified lipids, and the major respiratory quinone was MK-6. The predominant fatty acids were C16  :  1ω7c and/or C16  :  1ω6c and iso-C15  :  0. The DNA G+C content of the strain was 36 mol  %. Based on these data, strain T3T represents a novel species of the genus Flavobacterium, for which the name Flavobacterium procerum sp. nov. is proposed. The type strain is T3T ( = CGMCC 1.12926T = JCM 30113T).

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