scholarly journals Actinoplanes liguriensis sp. nov. and Actinoplanes teichomyceticus sp. nov.

2006 ◽  
Vol 56 (9) ◽  
pp. 2125-2130 ◽  
Author(s):  
Joachim M. Wink ◽  
Reiner M. Kroppenstedt ◽  
Peter Schumann ◽  
Gerhard Seibert ◽  
Erko Stackebrandt
Keyword(s):  
16S Rrna ◽  
Type Strain ◽  
Novel Species ◽  
Gene Tree ◽  
Taxonomic Status ◽  
Rrna Gene ◽  
Actinoplanes Teichomyceticus ◽  
Physiological Properties ◽  
Phylogenetic Lineages ◽  
The 16S Rrna Gene

The taxonomic status of ‘Actinoplanes liguriae’ A/6353 and ‘Actinoplanes teichomyceticus’ AB8327 was established by using a polyphasic approach. Strains A/6353 and AB8327 form distinct phylogenetic lineages in the 16S rRNA gene tree of members of the genus Actinoplanes and are related moderately and closely to Actinoplanes rectilineatus and Actinoplanes cyaneus, respectively. Morphological, cultural and physiological properties indicated that strains A/6353 and AB8327 represent separate, novel species of the genus Actinoplanes, Actinoplanes liguriensis sp. nov. (type strain A/6353T=FH 2244T=DSM 43865T=ATCC 31048T=BCRC 12121T=CBS 355.75T=IMSNU 22127T=JCM 3250T=KCTC 9536T=KCC A-0250T=NBRC 13997T=NCIMB 12636T=NRRL B-16723T=SANK 62178T) and Actinoplanes teichomyceticus sp. nov. (type strain AB8327T=FH 2149T=DSM 43866T=ATCC 31121T=BCRC 12106T=FERM P-3462T=IMSNU 20043T=IMET 9254T=JCM 3252T=KCC A-0252T=KCTC 9543T=NBRC 13999T=NCIMB 12640T=NRRL B-16726T=SANK 60479T).

scholarly journals Reclassification of Streptomyces hygroscopicus strains as Streptomyces aldersoniae sp. nov., Streptomyces angustmyceticus sp. nov., comb. nov., Streptomyces ascomycinicus sp. nov., Streptomyces decoyicus sp. nov., comb. nov., Streptomyces milbemycinicus sp. nov. and Streptomyces wellingtoniae sp. nov.

2010 ◽  
Vol 60 (4) ◽  
pp. 769-775 ◽  
Author(s):  
Yashawant Kumar ◽  
Michael Goodfellow
Keyword(s):  
16S Rrna ◽  
Type Strain ◽  
Novel Species ◽  
Gene Tree ◽  
Taxonomic Status ◽  
Streptomyces Hygroscopicus ◽  
Morphological Properties ◽  
Rrna Gene ◽  
Phenotypic Properties ◽  
Phenotypic Data

A polyphasic study was undertaken to determine the taxonomic status of six strains received as Streptomyces hygroscopicus. The strains had chemotaxonomic and morphological properties typical of members of the genus Streptomyces and formed distinct phyletic lines in the Streptomyces 16S rRNA gene tree. These strains were distinguished from one another and from phylogenetically close neighbours using a combination of phenotypic properties. The combined genotypic and phenotypic data showed that all six strains form distinct centres of taxonomic variation within the genus Streptomyces. The following novel species are proposed to accommodate the strains: Streptomyces aldersoniae sp. nov. (type strain DSM 41909T =NRRL 18513T), Streptomyces angustmyceticus sp. nov., comb. nov. (type strain DSM 41683T=NRRL B-2347T), Streptomyces ascomycinicus sp. nov. (type strain DSM 40822T =NBRC 13981T), Streptomyces decoyicus sp. nov., comb. nov. (type strain DSM 41427T =NRRL 2666T), Streptomyces milbemycinicus sp. nov. (type strain DSM 41911T =NRRL 5739T) and Streptomyces wellingtoniae sp. nov. (type strain DSM 40632T =NRRL B-1503T).

Taxonomic status of the species Clostridium methoxybenzovorans Mechichi et al. 1999

2021 ◽  
Vol 71 (8) ◽  
Author(s):  
Hisami Kobayashi ◽  
Yasuhiro Tanizawa ◽  
Mitsuo Sakamoto ◽  
Moriya Ohkuma ◽  
Masanori Tohno
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Strain ◽  
Type Species ◽  
Taxonomic Status ◽  
Rrna Gene ◽  
Whole Genome ◽  
Content Type ◽  
Link Type ◽  
The 16S Rrna Gene

The taxonomic status of the species Clostridium methoxybenzovorans was assessed. The 16S rRNA gene sequence, whole-genome sequence and phenotypic characterizations suggested that the type strain deposited in the American Type Culture Collection ( C. methoxybenzovorans ATCC 700855T) is a member of the species Eubacterium callanderi . Hence, C. methoxybenzovorans ATCC 700855T cannot be used as a reference for taxonomic study. The type strain deposited in the German Collection of Microorganism and Cell Cultures GmbH (DSM 12182T) is no longer listed in its online catalogue. Also, both the 16S rRNA gene and the whole-genome sequences of the original strain SR3T showed high sequence identity with those of Lacrimispora indolis (recently reclassified from Clostridium indolis ) as the most closely related species. Analysis of the two genomes showed average nucleotide identity based on blast and digital DNA–DNA hybridization values of 98.3 and 87.9 %, respectively. Based on these results, C. methoxybenzovorans SR3T was considered to be a member of L. indolis .

scholarly journals Kocuria marina sp. nov., a novel actinobacterium isolated from marine sediment

2004 ◽  
Vol 54 (5) ◽  
pp. 1617-1620 ◽  
Author(s):  
Seung Bum Kim ◽  
Olga I. Nedashkovskaya ◽  
Valery V. Mikhailov ◽  
Suk Kyun Han ◽  
Kyoung-Ok Kim ◽  
...  
Keyword(s):  
Marine Sediment ◽  
Type Strain ◽  
Novel Species ◽  
Gene Tree ◽  
Growth Media ◽  
Rrna Gene ◽  
Phenotypic Characteristics ◽  
Peter The Great ◽  
East Siberian Sea ◽  
The 16S Rrna Gene

An actinobacterial strain was isolated from marine sediment taken from the Troitsa Bay of the Gulf of Peter the Great, East Siberian Sea, and subjected to a taxonomic investigation. The isolate, designated KMM 3905T, was Gram-positive, aerobic, non-motile and coccoid. The strain tolerated up to 15 % NaCl in growth media, although its presence was not essential for growth. Chemotaxonomic and phylogenetic properties of the strain were consistent with its classification in the genus Kocuria. The strain formed an independent phylogenetic line in the 16S rRNA gene tree encompassed by members of Kocuria. Comparison of phenotypic characteristics also supported its assignment to a novel species within Kocuria. The name Kocuria marina sp. nov. (type strain KMM 3905T=KCTC 9943T) is proposed for this halotolerant actinobacterium from the marine environment.

scholarly journals Williamsia marianensis sp. nov., a novel actinomycete isolated from the Mariana Trench

2006 ◽  
Vol 56 (5) ◽  
pp. 1123-1126 ◽  
Author(s):  
Wasu Pathom-aree ◽  
Yuichi Nogi ◽  
Iain C. Sutcliffe ◽  
Alan C. Ward ◽  
Koki Horikoshi ◽  
...  
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Gene Tree ◽  
Taxonomic Status ◽  
Morphological Properties ◽  
Rrna Gene ◽  
Hexadecenoic Acid ◽  
Phenotypic Data ◽  
Mariana Trench ◽  
Phyletic Line

The taxonomic status of an actinomycete isolated from sediment collected from the Mariana Trench was established using a combination of genotypic and phenotypic data. Isolate MT8T had chemotaxonomic and morphological properties consistent with its classification in the genus Williamsia, and formed a distinct phyletic line in the 16S rRNA gene tree together with the type strain of Williamsia muralis. The isolate was readily distinguished from the latter, and from representatives of other Williamsia species, using DNA–DNA relatedness and phenotypic criteria. Predominant cellular fatty acids were oleic, palmitic and tuberculostearic acids and a hexadecenoic acid. The DNA G+C content was 65.2 mol%. It is apparent that the isolate belongs to a novel species of Williamsia. Strain MT8T (=DSM 44944T=NCIMB 14085T) was thus considered to be the type strain of a novel species in the genus Williamsia, for which the name Williamsia marianensis sp. nov. is proposed.

scholarly journals Neutrotolerant acidophilic Streptomyces species isolated from acidic soils in China: Streptomyces guanduensis sp. nov., Streptomyces paucisporeus sp. nov., Streptomyces rubidus sp. nov. and Streptomyces yanglinensis sp. nov.

2006 ◽  
Vol 56 (5) ◽  
pp. 1109-1115 ◽  
Author(s):  
Chunguang Xu ◽  
Liming Wang ◽  
Qingfeng Cui ◽  
Ying Huang ◽  
Zhiheng Liu ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Strain ◽  
Phylogenetic Trees ◽  
Gene Tree ◽  
Taxonomic Status ◽  
Rrna Gene ◽  
Acidic Soils ◽  
Phenotypic Data ◽  
Streptomyces Species

The taxonomic status of six neutrotolerant acidophilic streptomycetes isolated from acidic soils in Yunnan Province, China, was established using a polyphasic approach. The morphological and chemotaxonomic characteristics revealed that the isolates belong to the genus Streptomyces. Almost complete 16S rRNA gene sequences of the isolates were determined and aligned with available corresponding sequences of representatives of the family Streptomycetaceae; phylogenetic trees were inferred using four tree-making algorithms. The isolates formed a distinct, albeit heterogeneous, subclade in the Streptomyces 16S rRNA gene tree together with the type strain of Streptomyces yeochonensis, but were readily distinguishable from the latter using DNA–DNA hybridization and phenotypic data. It was evident from the genotypic and phenotypic data that the isolates belonged to four novel Streptomyces species, for which the following names are proposed: Streptomyces guanduensis sp. nov. (type strain 701T=CGMCC 4.2022T=JCM 13274T), Streptomyces paucisporeus sp. nov. (type strain 1413T=CGMCC 4.2025T=JCM 13276T), Streptomyces rubidus sp. nov. (type strain 13c15T=CGMCC 4.2026T=JCM 13277T) and Streptomyces yanglinensis sp. nov. (type strain 1307T=CGMCC 4.2023T=JCM 13275T); isolates 317 and 913 belong to this latter species.

scholarly journals Glycomyces sambucus sp. nov., an endophytic actinomycete isolated from the stem of Sambucus adnata Wall

2007 ◽  
Vol 57 (9) ◽  
pp. 1995-1998 ◽  
Author(s):  
Qiang Gu ◽  
Wen Zheng ◽  
Ying Huang
Keyword(s):  
Type Strain ◽  
Gene Sequence ◽  
Novel Species ◽  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Physiological Properties ◽  
Phyletic Line ◽  
The 16S Rrna Gene ◽  
Chinese Medicinal Plant

An actinomycete, designated strain E71T, was isolated from the stem of Sambucus adnata Wall, a Chinese medicinal plant, and subjected to a polyphasic taxonomic study. Phylogenetic analyses based on the 16S rRNA gene sequence showed that the organism was a member of the genus Glycomyces, and formed a distinct phyletic line distantly related to recognized species of the genus Glycomyces. Morphological and chemotaxonomic data supported the affiliation of strain E71T to the genus Glycomyces. A number of physiological properties and a unique menaquinone profile allowed differentiation of the strain from related Glycomyces species. It is therefore proposed that strain E71T represents a novel species of the genus Glycomyces, for which the name Glycomyces sambucus sp. nov. is proposed. The type strain is E71T (=CGMCC 4.3147T=DSM 45047T).

scholarly journals Reclassification of Paralactobacillus selangorensis Leisner et al. 2000 as Lactobacillus selangorensis comb. nov.

2011 ◽  
Vol 61 (12) ◽  
pp. 2979-2983 ◽  
Author(s):  
Monique Haakensen ◽  
Vanessa Pittet ◽  
Barry Ziola
Keyword(s):  
Cell Division ◽  
Sequence Analysis ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Strain ◽  
Taxonomic Status ◽  
Multilocus Sequence Analysis ◽  
Rrna Gene ◽  
Phenotypic Information ◽  
The 16S Rrna Gene

The taxonomic status of Paralactobacillus selangorensis is described and, based on evidence presented, transfer of the species to the genus Lactobacillus with the name Lactobacillus selangorensis comb. nov. is proposed. This reclassification is supported by multilocus sequence analysis of the 16S rRNA gene and portions of the cpn60, pheS and rpoA genes. Mode of cell division and existing phenotypic information also show that P. selangorensis cannot be differentiated from the genus Lactobacillus. The type strain of Lactobacillus selangorensis comb. nov. is ATCC BAA-66T ( = LMG 17710T  = CIP 106482T).

scholarly journals Haloferax profundi sp. nov. and Haloferax marisrubri sp. nov., Isolated from the Discovery Deep Brine-Seawater Interface in the Red Sea

2020 ◽  
Vol 8 (10) ◽  
pp. 1475
Author(s):  
Guishan Zhang ◽  
Xiaoyan Dong ◽  
Yingjiao Sun ◽  
André Antunes ◽  
Tyas Hikmawan ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Red Sea ◽  
Type Strain ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Gram Negative ◽  
The 16S Rrna Gene

Two extremely halophilic archaeal strains, designated SB29T and SB3T, were isolated from the brine-seawater interface of Discovery Deep in the Red Sea. Cells of both strains were pleomorphic (irregular polyhedrals, ovals, and rods) and stained Gram-negative; colonies were pigmented pink. The sequence similarity of the 16S rRNA gene of strain SB29T with that of its most closely related validly described species (Hfx. sulfurifontis DSM 16227T) and that of strain SB3T with its closest validly described relative (Hfx. denitrificans ATCC 35960T) was 98.1% and 98.6%, respectively. The incomplete draft genomes of SB29T and SB3T are 3,871,125 bp and 3,904,985 bp in size, respectively, and their DNA G + C contents are 60.75% and 65.64%, respectively. The highest ANI values between the genomes of SB29T and SB3T and the most closely related genomes in GenBank were determined as 82.6% (Hfx. sulfurifontis ATCC BAA-897T, GenBank accession no. GCA_000337835.1) and 92.6% (Haloferax denitrificans ATCC 35960T, GenBank accession no. GCA_000337795.1), respectively. These data indicate that the two new isolates cannot be classified into any recognized species of the genus Haloferax, and, therefore, two novel species of the genus Haloferax are proposed: Haloferax profundi sp. nov. (type strain SB29T = JCM 19567T = CGMCC 1.14960T) and Haloferax marisrubri sp. nov. (type strain SB3T = JCM 19566T = CGMCC 1.14958T).

scholarly journals Williamsia faeni sp. nov., an actinomycete isolated from a hay meadow

2010 ◽  
Vol 60 (11) ◽  
pp. 2548-2551 ◽  
Author(s):  
Amanda L. Jones ◽  
Gail D. Payne ◽  
Michael Goodfellow
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Gene Tree ◽  
Taxonomic Status ◽  
Rrna Gene ◽  
Phenotypic Properties ◽  
Phyletic Line ◽  
Type Strains ◽  
The 16S Rrna Gene ◽  
Sequence Similarities

The taxonomic status of an actinomycete isolated from soil collected from a hay meadow was determined using a polyphasic approach. The strain, designated N1350T, had morphological and chemotaxonomic properties consistent with its classification in the genus Williamsia and formed a distinct phyletic line within the clade comprising the type strains of species of the genus Williamsia in the 16S rRNA gene tree. Strain N1350T shared highest 16S rRNA gene sequence similarities with Williamsia marianensis MT8T (98.1 %) and Williamsia muralis MA140-96T (98.3 %). However, strain N1350T was readily distinguished from the type strains of Williamsia species using a combination of phenotypic properties. On the basis of these data, strain N1350T is considered to represent a novel species of the genus Williamsia. The name proposed for this taxon is Williamsia faeni sp. nov., with the type strain N1350T (=DSM 45372T =NCIMB 14575T =NRRL B-24794T).

Streptococcus vicugnae sp. nov., isolated from faeces of alpacas (Vicugna pacos) and cattle (Bos taurus), Streptococcus zalophi sp. nov., and Streptococcus pacificus sp. nov., isolated from respiratory tract of California sea lions (Zalophus californianus)

2021 ◽  
Vol 71 (5) ◽  
Author(s):  
Dmitriy V. Volokhov ◽  
Tatiana A. Zagorodnyaya ◽  
Zhenyu Shen ◽  
Jochen Blom ◽  
Vyacheslav A. Furtak ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Strain ◽  
Type Species ◽  
Novel Species ◽  
Rrna Gene ◽  
California Sea Lions ◽  
Content Type ◽  
Link Type ◽  
The 16S Rrna Gene

Four novel independent strains of Streptococcus spp. were isolated from faeces of alpaca (SL1232T), cattle (KCJ4950), and from respiratory tract of wild California sea lions (CSL7508T, CSL7591T). The strains were indole-, oxidase- and catalase-negative, non-spore-forming, non-motile Gram-positive cocci in short and long chains, facultative anaerobes. The 16S rRNA gene of SL1232T and KCJ4950 shared 99.40–99.60% nucleotide similarity to strains of S. equinus, S. lutetiensis, S. infantarius, and the 16S rRNA gene of CSL7508T and CSL7591T demonstrated 98.72 and 98.92% similarity, respectively, to S. marimammalium . All other known Streptococcus species had the 16S rRNA gene sequence similarities of ≤95%. The genomes were sequenced for the novel strains. Average nucleotide identity (ANI) analysis for strains SL1232T and KCJ4950, showed the highest similarity to S. equinus, S. lutetiensis, and S. infantarius with 85.21, 87.17, 88.47, 85.54, 87.47 and 88.89%, respectively, and strains CSL7508T and CSL7591T to S. marimammalium with 87.16 and 83.97%, respectively. Results of ANI were confirmed by pairwise digital DNA–DNA hybridization and phylogeny, which also revealed that the strains belong to three novel species of the genus Streptococcus . Phenotypical features of the novel species were in congruence with closely related members of the genus Streptococcus and gave negative reactions with the tested Lancefield serological groups (A-D, F and G). MALDI-TOF mass spectrometry supported identification of the species. Based on these data, we propose three novel species of the genus Streptococcus , for which the name Streptococcus vicugnae sp. nov. is proposed with the type strain SL1232T (=NCTC 14341T=DSM 110741T=CCUG 74371T), Streptococcus zalophi sp. nov. is proposed with the type strain CSL7508T (=NCTC 14410T=DSM 110742T=CCUG 74374T) and Streptococcus pacificus sp. nov. is proposed with the type strain CSL7591T (=NCTC 14455T=DSM 111148T=CCUG 74655T). The genome G+C content is 36.89, 34.85, and 35.34 % and draft genome sizes are 1906993, 1581094 and 1656080 bp for strains SL1232T, CSL7508T, and CSL7591T, respectively.

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