scholarly journals Haloplanus vescus sp. nov., an extremely halophilic archaeon from a marine solar saltern, and emended description of the genus Haloplanus

2010 ◽  
Vol 60 (8) ◽  
pp. 1824-1827 ◽  
Author(s):  
Heng-Lin Cui ◽  
Xia Gao ◽  
Xin-Yi Li ◽  
Xue-Wei Xu ◽  
Yu-Guang Zhou ◽  
...  
Keyword(s):  
Surface Strain ◽  
Rrna Gene ◽  
Solar Saltern ◽  
Halophilic Archaeon ◽  
Marine Solar Saltern ◽  
Gene Sequence Analysis ◽  
Nacl Concentration ◽  
Optimum Ph ◽  
Emended Description ◽  
Extremely Halophilic Archaeon

An extremely halophilic archaeon, strain RO5-8T, was isolated from a disused marine solar saltern in China. The cells were pleomorphic and flat. In static liquid medium, cells floated to the surface. Strain RO5-8T stained Gram-negative and colonies were pink-pigmented. It was able to grow at 30–50 °C (optimum 40 °C), at 2.6–4.3 M NaCl (optimum 3.1 M NaCl), at 0.03–0.5 M MgCl2 (optimum 0.03 M MgCl2) and at pH 5.5–7.5 (optimum pH 6.0–6.5). Cells lysed in distilled water and the minimal NaCl concentration to prevent cell lysis was 12 % (w/v). The major polar lipids of strain RO5-8T were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and one major glycolipid chromatographically identical to the sulfated mannosyl glucosyl diether S-DGD-1. On the basis of 16S rRNA gene sequence analysis, strain RO5-8T was closely related to three strains of Haloplanus natans with similarities of 97.3–97.6 %. The DNA G+C content of strain RO5-8T was 62.1 mol%. The DNA–DNA hybridization value between strain RO5-8T and Haloplanus natans JCM 14081T was 51.6 %. It was concluded that strain RO5-8T represents a novel species of the genus Haloplanus, for which the name Haloplanus vescus sp. nov. is proposed. The type strain is RO5-8T (=CGMCC 1.8712T =JCM 16055T).

scholarly journals Halogeometricum rufum sp. nov., a halophilic archaeon from a marine solar saltern, and emended description of the genus Halogeometricum

2010 ◽  
Vol 60 (11) ◽  
pp. 2613-2617 ◽  
Author(s):  
Heng-Lin Cui ◽  
Xin Yang ◽  
Xia Gao ◽  
Xin-Yi Li ◽  
Xue-Wei Xu ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Halophilic Archaea ◽  
Rrna Gene ◽  
Solar Saltern ◽  
Marine Solar Saltern ◽  
Gene Sequence Analysis ◽  
Nacl Concentration ◽  
Optimum Ph ◽  
Emended Description ◽  
Halogeometricum Borinquense

Two halophilic archaea, strains RO1-4T and RO1-64, were isolated from a marine solar saltern in Jiangsu, China. Cells of the two strains were pleomorphic, motile, and stained Gram-negative. Colonies were red-pigmented. Strains RO1-4T and RO1-64 were able to grow at 25–55 °C (optimum 40–42 °C), at 2.1–5.1 M NaCl (optimum 3.9 M NaCl), at 0.05–0.7 M MgCl2 (optimum 0.3 M MgCl2) and at pH 6.0–8.5 (optimum pH 7.0). Cells lyse in distilled water and the minimal NaCl concentration to prevent cell lysis is 12 % (w/v). On the basis of 16S rRNA gene sequence analysis, strains RO1-4T and RO1-64 were closely related to Halogeometricum borinquense PR3T (98.0 and 98.2 % similarity, respectively) and Halosarcina pallida BZ256T (97.8 and 97.9 %). The major polar lipids of the two strains were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and two glycolipids (S-DGD-1 and DGD-1). The DNA G+C contents of strains RO1-4T and RO1-64 are 64.9 and 62.4 mol%, respectively. The DNA–DNA hybridization value between strains RO1-4T and RO1-64 was 83.0 % and both strains showed low DNA–DNA relatedness with Halogeometricum borinquense PR3T (42.5 and 50.1 % relatedness, respectively) and Halosarcina pallida BZ256T (37.6 and 42.1 % relatedness). It was concluded that strains RO1-4T and RO1-64 represent a novel species of the genus Halogeometricum, for which the name Halogeometricum rufum sp. nov. is proposed. The type strain is RO1-4T (=CGMCC 1.7736T =JCM 15770T).

scholarly journals Haloplanus aerogenes sp. nov., an extremely halophilic archaeon from a marine solar saltern

2011 ◽  
Vol 61 (4) ◽  
pp. 965-968 ◽  
Author(s):  
Heng-Lin Cui ◽  
Xia Gao ◽  
Xin Yang ◽  
Xue-Wei Xu
Keyword(s):  
Dna Hybridization ◽  
Gene Sequence ◽  
Rrna Gene ◽  
Solar Saltern ◽  
Gas Vesicles ◽  
Marine Solar Saltern ◽  
Gene Sequence Analysis ◽  
Nacl Concentration ◽  
Optimum Ph ◽  
Extremely Halophilic Archaeon

Halophilic archaeal strain TBN37T was isolated from Taibei marine solar saltern near Lianyungang city of Jiangsu province, China. Cells were pleomorphic, flat and contained gas vesicles. Cells of strain TBN37T stained Gram-negative and the colonies were pink-pigmented. The strain was able to grow at 25–50 °C (optimum, 37–40 °C), with 1.4–5.1 M NaCl (optimum, 2.1 M NaCl), with 0–1.0 M MgCl2 (optimum, 0.01 M MgCl2) and at pH 6.0–9.0 (optimum, pH 7.5). Cells lysed in distilled water and the minimal NaCl concentration to prevent cell lysis was 8 % (w/v). The major polar lipids of strain TBN37T were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and one major glycolipid chromatographically identical to sulfated mannosyl glucosyl diether (S-DGD-1). On the basis of 16S rRNA gene sequence analysis, strain TBN37T was closely related to Haloplanus natans and Haloplanus vescus, with the same similarity of 97.4 %. The DNA G+C content of strain TBN37T is 64.1 mol%. DNA–DNA hybridization values between strain TBN37T and Haloplanus natans JCM 14081T and between strain TBN37T and Haloplanus vescus RO5-8T were 37.6 % and 42.1 %, respectively. It was concluded that strain TBN37T represents a novel species of the genus Haloplanus, for which the name Haloplanus aerogenes sp. nov. is proposed. The type strain is TBN37T ( = CGMCC 1.10124T  = JCM 16430T).

scholarly journals Halorubrum litoreum sp. nov., an extremely halophilic archaeon from a solar saltern

2007 ◽  
Vol 57 (10) ◽  
pp. 2204-2206 ◽  
Author(s):  
Heng-Lin Cui ◽  
Ze-Ying Lin ◽  
Ying Dong ◽  
Pei-Jin Zhou ◽  
Shuang-Jiang Liu
Keyword(s):  
Dna Hybridization ◽  
Gene Sequence ◽  
Novel Species ◽  
Rrna Gene ◽  
Solar Saltern ◽  
Halophilic Archaeon ◽  
Marine Solar Saltern ◽  
Gene Sequence Analysis ◽  
Extremely Halophilic Archaeon ◽  
Sequence Similarities

An extremely halophilic archaeon, strain Fa-1T, was isolated from a marine solar saltern in Fujian, China. Strain Fa-1T required Mg2+ and at least 2.0 M NaCl for growth. It was able to grow at pH 6.5–9.0 (optimally at pH 7.0–7.5) and at 20–55 °C (optimally at 37–42 °C). The major polar lipids of strain Fa-1T were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and a sulfated diglycosyl diether. On the basis of a 16S rRNA gene sequence analysis, strain Fa-1T was closely related to nine species of the genus Halorubrum, showing sequence similarities of 97.4–98.4 %. The G+C content of the DNA of strain Fa-1T is 64.9 mol% (T m). DNA–DNA hybridization values between strain Fa-1T and the most closely related members of the genus Halorubrum were below 51 %. On the basis of the data from this study, strain Fa-1T represents a novel species of the genus Halorubrum, for which the name Halorubrum litoreum sp. nov. is proposed. The type strain is Fa-1T (=CGMCC 1.5336T =JCM 13561T).

scholarly journals Haladaptatus litoreus sp. nov., an extremely halophilic archaeon from a marine solar saltern, and emended description of the genus Haladaptatus

2010 ◽  
Vol 60 (5) ◽  
pp. 1085-1089 ◽  
Author(s):  
Heng-Lin Cui ◽  
Fang-Fang Sun ◽  
Xia Gao ◽  
Ying Dong ◽  
Xue-Wei Xu ◽  
...  
Keyword(s):  
Halophilic Archaea ◽  
Single Species ◽  
Rrna Gene ◽  
Solar Saltern ◽  
Marine Solar Saltern ◽  
Gene Sequence Analysis ◽  
Emended Description ◽  
Extremely Halophilic Archaeon ◽  
Low Levels ◽  
Ph Range

Two extremely halophilic archaea, strains RO1-28T and RO1-22, were isolated from a marine solar saltern in Jiangsu, China. Both strains required at least 0.05 M Mg2+ and 1.7 M NaCl for growth. They were able to grow over a pH range of 6.0–8.5 and a temperature range of 25–55 °C, with optimal pH of 7.0 and optimal temperature of 37–40 °C. Based on 16S rRNA gene sequence analysis, strains RO1-28T and RO1-22 were closely related to Haladaptatus paucihalophilus, the single species of the genus Haladaptatus, with similarities of 94.0–95.2 %. The major polar lipids of the two strains were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and three glycolipids chromatographically identical to the glycolipids of Haladaptatus paucihalophilus JCM 13897T. Both strains RO1-28T and RO1-22 had a DNA G+C content of 54.0 mol% (HPLC). The DNA–DNA hybridization value between the two strains was more than 70 % (92 %) and both strains showed low levels of DNA–DNA relatedness (32 % and 33 %) with Haladaptatus paucihalophilus JCM 13897T. It was concluded that strains RO1-28T and RO1-22 represent a novel species of the genus Haladaptatus, for which the name Haladaptatus litoreus sp. nov. is proposed. The type strain is RO1-28T (=CGMCC 1.7737T =JCM 15771T).

scholarly journals Halogranum gelatinilyticum sp. nov. and Halogranum amylolyticum sp. nov., isolated from a marine solar saltern, and emended description of the genus Halogranum

2011 ◽  
Vol 61 (4) ◽  
pp. 911-915 ◽  
Author(s):  
Heng-Lin Cui ◽  
Xin Yang ◽  
Xia Gao ◽  
Xue-Wei Xu
Keyword(s):  
Type Strain ◽  
Gene Sequence ◽  
Novel Species ◽  
Rrna Gene ◽  
Solar Saltern ◽  
Marine Solar Saltern ◽  
Dna Relatedness ◽  
Gene Sequence Analysis ◽  
Optimum Ph ◽  
Emended Description

Two extremely halophilic archaeal strains, designated TNN44T and TNN58T, were isolated from Tainan marine solar saltern near Lianyungang city, Jiangsu province, China. Cells of the two strains were pleomorphic and Gram-stain-negative; colonies were red-pigmented. Strains TNN44T and TNN58T were able to grow at 20–50 °C (optimum 37 °C for both), in the presence of 1.4–5.1 M NaCl (optimum 3.4–3.9 M NaCl) and at pH 5.5–9.0 (optimum pH 6.5–7.0); neither strain required Mg2+ for growth. Cells lysed in distilled water. On the basis of 16S rRNA gene sequence analysis, strains TNN44T and TNN58T were related closely to Halogranum rubrum RO2-11T (96.2 and 97.2 % similarity, respectively). The polar lipids of the two strains were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate (trace), and one major glycolipid and one minor glycolipid chromatographically identical to sulfated mannosyl glucosyl diether and mannosyl glucosyl diether, respectively; other trace unidentified lipids were also detected. The DNA G+C content of strains TNN44T and TNN58T was 64.0 and 62.0 mol%, respectively. The level of DNA–DNA relatedness between strains TNN44T and TNN58T was 37.2 %, and these two strains showed a low level of DNA–DNA relatedness with Halogranum rubrum RO2-11T (40.6 and 44.4 %, respectively). Two novel species of the genus Halogranum are proposed to accommodate these two strains, Halogranum gelatinilyticum sp. nov. (type strain TNN44T  = CGMCC 1.10119T  = JCM 16426T) and Halogranum amylolyticum sp. nov. (type strain TNN58T  = CGMCC 1.10121T  = JCM 16428T).

scholarly journals Halosarcina limi sp. nov., a halophilic archaeon from a marine solar saltern, and emended description of the genus Halosarcina

2010 ◽  
Vol 60 (10) ◽  
pp. 2462-3466 ◽  
Author(s):  
Heng-Lin Cui ◽  
Xia Gao ◽  
Xin-Yi Li ◽  
Xue-Wei Xu ◽  
Yu-Guang Zhou ◽  
...  
Keyword(s):  
Eastern China ◽  
Phenotypic Characterization ◽  
Rrna Gene ◽  
Solar Saltern ◽  
Halophilic Archaeon ◽  
Marine Solar Saltern ◽  
The Family ◽  
Nacl Concentration ◽  
Type Strains ◽  
The 16S Rrna Gene

A halophilic archaeon, strain RO1-6T, was isolated from a marine solar saltern in eastern China. Cells of strain RO1-6T were pleomorphic and motile and stained Gram-negative. Strain RO1-6T grew well on complex medium and colonies were red-pigmented. It was able to grow at 20–50 °C (optimum 37 °C), in 2.1–5.1 M NaCl (optimum 3.9 M NaCl), in 0.05–0.70 M MgCl2 (optimum 0.30 M MgCl2) and at pH 6.5–8.0 (optimum pH 7.0). Cells lysed in distilled water and the minimal NaCl concentration to prevent cell lysis was 12 % (w/v). The major polar lipids of strain RO1-6T were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and two glycolipids that were chromatographically identical to S-DGD-1 and S2-DGD. The 16S rRNA gene sequence of strain RO1-6T showed similarities of 96.9 and 96.4 % to those of the type strains of Halosarcina pallida and Halogeometricum borinquense, respectively, members of the most closely related recognized genera within the family Halobacteriaceae. The DNA G+C content of strain RO1-6T was 61.2 mol%. Phenotypic characterization and phylogenetic analysis revealed that strain RO1-6T is related to Halosarcina pallida and represents a novel species of the genus Halosarcina, for which the name Halosarcina limi sp. nov. is proposed; the type strain is RO1-6T (=CGMCC 1.8711T =JCM 16054T).

scholarly journals Natronorubrum aibiense sp. nov., an extremely halophilic archaeon isolated from Aibi salt lake in Xin-Jiang, China, and emended description of the genus Natronorubrum

2006 ◽  
Vol 56 (7) ◽  
pp. 1515-1517 ◽  
Author(s):  
Heng-Lin Cui ◽  
Dilbr Tohty ◽  
Jie Feng ◽  
Pei-Jin Zhou ◽  
Shuang-Jiang Liu
Keyword(s):  
Gene Sequence ◽  
Agar Medium ◽  
Novel Species ◽  
Salt Lake ◽  
Rrna Gene ◽  
Halophilic Archaeon ◽  
Optimum Ph ◽  
Emended Description ◽  
Extremely Halophilic Archaeon ◽  
Sequence Similarities

An extremely halophilic archaeon (strain 7-3T) was isolated from Aibi salt lake in Xin-Jiang, China. It formed small (<1.0 mm), red, elevated colonies on salt-milk agar medium. Strain 7-3T was able to grow at pH 6.5–9.5 (optimum pH 8.0) and at 20–50 °C (optimum 45 °C). Analysis of its 16S rRNA gene sequence indicated that strain 7-3T was phylogenetically related to members of the genus Natronorubrum, with sequence similarities of 97.0 % to Natronorubrum bangense A33T and 95.2 % to Natronorubrum tibetense GA33T. The G+C content of its DNA was 61.2 mol% (T m). Levels of DNA–DNA relatedness to Nrr. bangense and Nrr. tibetense were 45 and 37 %, respectively. It is concluded that strain 7-3T (=CGMCC 1.4299T=JCM 13488T) should be classified as the type strain of a novel species of the genus Natronorubrum, for which the name Natronorubrum aibiense sp. nov. is proposed. Based on the properties of Nrr. aibiense given here and of those of Nrr. bangense and Nrr. tibetense described previously, an emended description of the genus Natronorubrum is presented.

scholarly journals Halopelagius inordinatus gen. nov., sp. nov., a new member of the family Halobacteriaceae isolated from a marine solar saltern

2010 ◽  
Vol 60 (9) ◽  
pp. 2089-2093 ◽  
Author(s):  
Heng-Lin Cui ◽  
Xin-Yi Li ◽  
Xia Gao ◽  
Xue-Wei Xu ◽  
Yu-Guang Zhou ◽  
...  
Keyword(s):  
Halophilic Archaea ◽  
Rrna Gene ◽  
Solar Saltern ◽  
16S Rrna Gene Sequences ◽  
Marine Solar Saltern ◽  
The Family ◽  
Nacl Concentration ◽  
Optimum Ph ◽  
Extremely Halophilic Archaea ◽  
The 16S Rrna Gene

Two extremely halophilic archaea, strains RO5-2T and RO5-14, were isolated from Rudong marine solar saltern in Jiangsu, China. Cells of the two strains were pleomorphic, motile and stained Gram-negative. Colonies were red-pigmented. Strains RO5-2T and RO5-14 were able to grow at 20–50 °C (optimum 37 °C), at 2.6–4.8 M NaCl (optimum 3.4–3.9 M NaCl), at 0.03–0.7 M MgCl2 (optimum 0.5 M MgCl2) and at pH 5.5–8.0 (optimum pH 6.5–7.0). Cells lyse in distilled water and the minimal NaCl concentration to prevent cell lysis was 12 % (w/v). The major polar lipids of the two strains were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and two major glycolipids chromatographically identical to sulfated mannosyl glucosyl diether (S-DGD-1) and mannosyl glucosyl diether (DGD-1). The 16S rRNA gene sequences of strains RO5-2T and RO5-14 showed 93.4–93.8 % similarity to the closest cultivated relative, Halosarcina pallida. The DNA G+C content of strains RO5-2T and RO5-14 was 61.0 mol% and 59.9 mol%, respectively. The DNA–DNA relatedness between strains RO5-2T and RO5-14 was 86.0 %. The phenotypic, chemotaxonomic and phylogenetic properties suggest that strains RO5-2T and RO5-14 represent a novel species in a new genus within the family Halobacteriaceae, for which the name Halopelagius inordinatus gen. nov., sp. nov. is proposed. The type strain is RO5-2T (=CGMCC 1.7739T =JCM 15773T).

scholarly journals Planococcus salinarum sp. nov., isolated from a marine solar saltern, and emended description of the genus Planococcus

2010 ◽  
Vol 60 (4) ◽  
pp. 754-758 ◽  
Author(s):  
Jung-Hoon Yoon ◽  
So-Jung Kang ◽  
Soo-Young Lee ◽  
Ki-Hoon Oh ◽  
Tae-Kwang Oh
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Gene Sequence ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Solar Saltern ◽  
Rrna Gene Sequence ◽  
Marine Solar Saltern ◽  
Emended Description ◽  
Type Strains

A Gram-positive, non-motile and coccoid-, short rod- or rod-shaped bacterial strain, ISL-16T, was isolated from a marine solar saltern in Korea and its taxonomic position was investigated using a polyphasic taxonomic approach. Strain ISL-16T grew optimally at pH 7.0–8.0, at 30 °C and in the presence of 2 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain ISL-16T joined the cluster comprising species of the genus Planococcus. Its 16S rRNA gene sequence contained the same signature nucleotides as those defined for the genus Planococcus. Strain ISL-16T exhibited 16S rRNA gene sequence similarity values of 96.9–98.2 % to the type strains of species of the genus Planococcus. Strain ISL-16T contained MK-8 and MK-7 as the predominant menaquinones and anteiso-C15 : 0, C16 : 1 ω7c alcohol and anteiso-C17 : 0 as the major fatty acids. The DNA G+C content was 48.3 mol%. DNA–DNA relatedness values between strain ISL-16T and the type strains of species of the genus Planococcus were 15–28 %. Differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, enabled strain ISL-16T to be differentiated from recognized species of the genus Planococcus. On the basis of the data presented, strain ISL-16T is considered to represent a novel species of the genus Planococcus, for which the name Planococcus salinarum sp. nov. is proposed. The type strain is ISL-16T (=KCTC 13584T=CCUG 57753T). An emended description of the genus Planococcus is also given.

Haloplanus salinus sp. nov., an extremely halophilic archaeon from a Chinese marine solar saltern

2013 ◽  
Vol 195 (12) ◽  
pp. 799-803 ◽  
Author(s):  
Xing–Xing Qiu ◽  
Mei-Lin Zhao ◽  
Dong Han ◽  
Wen-Jiao Zhang ◽  
Heng-Lin Cui
Keyword(s):  
Solar Saltern ◽  
Halophilic Archaeon ◽  
Marine Solar Saltern ◽  
Extremely Halophilic Archaeon ◽  
Chinese Marine
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