Salipiger mucescens gen. nov., sp. nov., a moderately halophilic, exopolysaccharide-producing bacterium isolated from hypersaline soil, belonging to the α-Proteobacteria

Salipiger mucescens gen. nov., sp. nov. is a moderately halophilic, exopolysaccharide-producing, Gram-negative rod isolated from a hypersaline habitat in Murcia in south-eastern Spain. The bacterium is chemoheterotrophic and strictly aerobic (i.e. unable to grow under anaerobic conditions either by fermentation or by nitrate or fumarate respiration). It does not synthesize bacteriochlorophyll a. Catalase and phosphatase are positive. It does not produce acids from carbohydrates. It cannot grow with carbohydrates or amino acids as sole sources of carbon and energy. It grows best at 9–10 % w/v NaCl and requires the presence of Na+ but not Mg2+ or K+, although they do stimulate its growth somewhat when present. Its major fatty-acid component is 18 : 1ω7c (78·0 %). The predominant respiratory lipoquinone found in strain A3T is ubiquinone with ten isoprene units. The G+C content is 64·5 mol%. Phylogenetic analyses strongly indicate that this strain forms a distinct line within a clade containing the genus Roseivivax in the subclass α-Proteobacteria. The similarity value with Roseivivax halodurans and Roseivivax halotolerans is 94 %. In the light of the polyphasic evidence gathered in this study it is proposed that the isolate be classified as representing a new genus and species, Salipiger mucescens gen. nov., sp. nov. The proposed type strain is strain A3T (=CECT 5855T=LMG 22090T=DSM 16094T).

Download Full-text

Molecular and morphological evidence reveal a new genus and species in Auriculariales from tropical China

MycoKeys ◽

10.3897/mycokeys.35.25271 ◽

2018 ◽

Vol 35 ◽

pp. 27-39 ◽

Cited By ~ 3

Author(s):

Hai-Sheng Yuan ◽

Xu Lu ◽

Cony Decock

Keyword(s):

New Species ◽

Dna Sequences ◽

Type Species ◽

New Genus ◽

Phylogenetic Analyses ◽

Strong Support ◽

Morphological Characteristics ◽

Morphological Evidence ◽

New Genus And Species ◽

Tropical China

Grammatuslabyrinthinusgen. et sp. nov. is proposed based on DNA sequences data and morphological characteristics. It is known so far from southern, tropical China. The new species is characterised by an annual, resupinate basidiocarp with a shallow, subporoid hymenophore, a hymenium restricted to the bottom of the tubes, a dimitic hyphal system, presence of encrusted skeletocystidia and dendrohyphidia, longitudinally septate basidia and smooth, oblong-ellipsoid to cylindrical, acyanophilous basidiospores. Phylogenetic analyses based on ITS + nLSU DNA sequences data indicate that G.labyrinthinus belongs to Auriculariaceae in which it has an isolated position. Phylogenetic inferences show G.labyrinthinus to be related to Heteroradulum. However, the ITS sequences similarity between G.labyrinthinus and H.kmetii, the type species of Heteroradulum, were 89.84% and support the establishment of the new genus. Inversely, Heteroradulumsemis clustered with G.labyrinthinus with strong support and it is transferred to Grammatus.

Download Full-text

Kinneretia asaccharophila gen. nov., sp. nov., isolated from a freshwater lake, a member of the Rubrivivax branch of the family Comamonadaceae

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY ◽

10.1099/ijs.0.011478-0 ◽

2010 ◽

Vol 60 (4) ◽

pp. 809-814 ◽

Cited By ~ 16

Author(s):

Margarita Gomila ◽

Jarone Pinhassi ◽

Enevold Falsen ◽

Edward R. B. Moore ◽

Jorge Lalucat

Keyword(s):

New Genus ◽

Cellular Fatty Acid ◽

Fatty Acid Analysis ◽

Strictly Aerobic ◽

Negative Bacterium ◽

New Genus And Species ◽

Biochemical Tests ◽

The Family ◽

Bacterium Strain ◽

Physiological And Biochemical

A strictly aerobic, Gram-negative bacterium, strain KIN192T, isolated from fresh water from Lake Kinneret, Israel, was examined using a polyphasic approach to characterize and clarify its phylogenetic and taxonomic position. Sequences of the 16S rRNA and gyrB genes and ITS1 revealed close relationships to species of the genera Pelomonas, Mitsuaria and Roseateles, in the Rubrivivax branch of the family Comamonadaceae of the Betaproteobacteria. Physiological and biochemical tests, cellular fatty acid analysis and DNA–DNA hybridizations indicated that this strain should be assigned to a new genus and species in the Rubrivivax phylogenetic branch, for which the name Kinneretia asaccharophila gen. nov., sp. nov., is proposed. The type strain of Kinneretia asaccharophila is strain KIN192T (=CCUG 53117T =CECT 7319T).

Download Full-text

A new genus and species of natricine snake from northeast India

Zootaxa ◽

10.11646/zootaxa.4603.2.2 ◽

2019 ◽

Vol 4603 (2) ◽

pp. 241 ◽

Cited By ~ 1

Author(s):

VARAD B. GIRI ◽

DAVID J. GOWER ◽

ABHIJIT DAS ◽

H.T. LALREMSANGA ◽

SAMUEL LALRONUNGA ◽

...

Keyword(s):

New World ◽

New Genus ◽

Phylogenetic Analyses ◽

East Asian ◽

Northeast India ◽

Molecular Variation ◽

New Genus And Species ◽

Indian Populations ◽

Western Palearctic ◽

Molecular Phylogenetic

Based on the first molecular phylogenetic analyses of samples from northeast India, specimens referred to Rhabdops from this region are more closely related to the southeast and east Asian natricine genera Opisthotropis Günther, 1872 and Sinonatrix Rossman & Eberle, 1977 (as well as to New World and western Palearctic natricines) than to peninsular Indian (true) Rhabdops. Morphologically, these northeast Indian populations differ from other natricines by having a single (‘fused’ or unpaired) internasal shield and a single prefrontal shield. Given the morphological and phylogenetic distinctiveness of these northeast Indian populations, we refer them to a new genus, Smithophis gen. nov., and transfer Rhabdops bicolor (Blyth, 1854) to Smithophis bicolor comb. nov. Based on morphological and molecular variation within our northeast Indian sample, we additionally describe Smithophis atemporalis sp. nov. from the state of Mizoram.

Download Full-text

Pinnule-less polyps: a new genus and new species of Indo-Pacific Clavulariidae and validation of the soft coral genus Acrossota and the family Acrossotidae (Coelenterata: Octocorallia)

Zootaxa ◽

10.11646/zootaxa.1400.2 ◽

2007 ◽

Vol 1400 (1) ◽

pp. 27 ◽

Cited By ~ 4

Author(s):

PHILP ALDERSLADE ◽

CATHERINE S. McFADDEN

Keyword(s):

New Genus ◽

Soft Coral ◽

Phylogenetic Analyses ◽

Mitochondrial Genes ◽

New Combination ◽

New Genus And Species ◽

Coral Genus ◽

The Family ◽

Lateral Margins ◽

Number Of Authors

Clavularia amboinensis Burchardt, a species described as possessing simple, pinnule-less tentacles (a fact refuted by later authors) is confirmed to be as described and is transferred as a new combination to Acrossota Bourne — a genus dismissed until now by a number of authors. The species is compared to recently collected material with live photographs. A second new genus and species, Knopia octocontacanalis, is also described. This taxon resembles Acrossota in general form, but has tentacles where the pinnules appear as though they are fused side to side along the tentacles’ lateral margins. Preliminary phylogenetic analyses of two mitochondrial genes support placement of Knopia in Clavulariidae and retention of genus Acrossota in Bourne’s unrecognised family Acrossotidae.

Download Full-text

Flavivirga jejuensis gen. nov., sp. nov., and Flavivirga amylovorans sp. nov., new members of the family Flavobacteriaceae isolated from seawater, and emended descriptions of the genera Psychroserpens and Lacinutrix

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY ◽

10.1099/ijs.0.033605-0 ◽

2012 ◽

Vol 62 (Pt_5) ◽

pp. 1061-1068 ◽

Cited By ~ 22

Author(s):

Hana Yi ◽

Jang-Cheon Cho ◽

Jongsik Chun

Keyword(s):

Type Strain ◽

New Genus ◽

Phylogenetic Analyses ◽

Gliding Motility ◽

Phenotypic Traits ◽

Rrna Gene ◽

Strictly Aerobic ◽

Content Type ◽

Link Type ◽

The Family

Two yellow, rod-shaped, Gram-reaction-negative, strictly aerobic bacterial strains, designated JC2682T and JC2681T, were isolated from a seawater sample of Jeju Island, Korea. The isolates required sea salts for growth and showed gliding motility. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strains JC2682T and JC2681T shared 96.9 % similarity and formed a robust monophyletic group within the radiation encompassing the family Flavobacteriaceae . This distinct clade, which had varying branch positions depending on the tree-making algorithm used, represents a new genus within the family Flavobacteriaceae . The predominant isoprenoid quinone (menaquinone 6, MK-6) and DNA G+C content (27–33 mol%) were consistent with its assignment to the family Flavobacteriaceae , but the polar lipid profile, fatty acid composition and overall phenotypic traits demonstrated that the test strains were not closely affiliated to any recognized genera. Based on data from the present polyphasic taxonomic study, strains JC2682T and JC2681T are considered to represent two novel species of a new genus in the family Flavobacteriaceae , for which the names Flavivirga jejuensis gen. nov., sp. nov. and Flavivirga amylovorans sp. nov. are proposed, respectively. The type strain of Flavivirga jejuensis is JC2682T ( = KACC 14158T = JCM 17113T). The type strain of Flavivirga amylovorans is JC2681T ( = KACC 14157T = JCM 17112T). Amended descriptions of the genera Psychroserpens and Lacinutrix are provided.

Download Full-text

Halobacillus sediminis sp. nov., a moderately halophilic bacterium isolated from a solar saltern sediment

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY ◽

10.1099/ijsem.0.000595 ◽

2015 ◽

Vol 65 (Pt_12) ◽

pp. 4434-4440 ◽

Cited By ~ 10

Author(s):

Su-Jin Kim ◽

Jae-Chan Lee ◽

Song-Ih Han ◽

Kyung-Sook Whang

Keyword(s):

Sequence Similarity ◽

Phylogenetic Analyses ◽

Halophilic Bacterium ◽

Rrna Gene ◽

Strictly Aerobic ◽

Solar Saltern ◽

Moderately Halophilic ◽

Gram Staining ◽

Moderately Halophilic Bacterium ◽

Type Strains

A Gram-staining-positive, moderately halophilic bacterium, designated strain NGS-2T, was isolated from sediment of a solar saltern pond located in Shinan, Korea. Strain NGS-2T was a strictly aerobic, non-motile rod that grew at pH 5.0–10.0 (optimum, pH 8.0), at 10–30 °C (optimum, 28 °C) and in the presence of 1–20 % (w/v) NaCl (optimum, 10 % NaCl). Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain NGS-2T belonged to the genus Halobacillus, with sequence similarity of 98.4–95.8 % to existing type strains, showing the highest sequence similarity to Halobacillus dabanensis D-8T (98.4 %), H. litoralis SL-4T (98.4 %), H. trueperi SL-5T (98.2 %), H. faecis IGA7-4T (98.2 %), H. profundi IS-Hb4T (98.1 %) and H. mangrovi MS10T (98.0 %). The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidyl-N-methylethanolamine and an unknown glycolipid. The cell-wall peptidoglycan was based on l-Orn–d-Asp, the predominant isoprenoid quinone was menaquinone 7 (MK-7) and the major fatty acids were anteiso-C15: 0 and anteiso-C17: 0. The DNA G+C content of the novel isolate was 45.0 mol%. Levels of DNA–DNA relatedness between strain NGS-2T and the type strains of 12 other species of the genus ranged from 32 to 3 %. On the basis of the polyphasic analysis conducted in this study, strain NGS-2T represents a novel species of the genus Halobacillus, for which the name Halobacillus sediminis sp. nov. is proposed. The type strain is NGS-2T ( = KACC 18263T = NBRC 110639T).

Download Full-text

The earliest mustelid in North America

Zoological Journal of the Linnean Society ◽

10.1093/zoolinnean/zlz091 ◽

2019 ◽

Cited By ~ 1

Author(s):

Ryan Paterson ◽

Joshua X Samuels ◽

Natalia Rybczynski ◽

Michael J Ryan ◽

Hillary C Maddin

Keyword(s):

New Species ◽

North America ◽

New Genus ◽

Phylogenetic Analyses ◽

Morphological Data ◽

Close Relative ◽

Late Oligocene ◽

New Genus And Species ◽

Holotype Specimen ◽

John Day

Abstract Until now, the pre-Miocene fossil record of mustelids in North America has been restricted to specimens attributable to oligobunine taxa and isolated remains tentatively allocated to the genus Plesictis. In the present study, we report on a nearly complete cranium and a referred dentary of a new genus and species of mustelid. The specimens were recovered from the Turtle Cove and Kimberly Members of the John Day Formation, Oregon, USA. These excellently preserved specimens more confidently confirm the presence of mustelids in the Early and Late Oligocene (Early and Late Arikareean) of North America. Like the holotype specimen of ‘Plesictis’ julieni, the new species lacks an alisphenoid canal and a postprotocrista on the M1 (synapomorphies of Mustelidae), but retains a dorsally deep suprameatal fossa (a feature occasionally suggested to be unique to Procyonidae). Phylogenetic analyses, applying parsimony and Bayesian inference to combined molecular (five genes totalling 5490 bp) and morphological data, recover this new species of mustelid as sister-species to ‘Plesictis’ julieni. The results of these analyses reveal that the new genus is a close relative of other species of Plesictis and several taxa traditionally allied with Oligobuninae, thereby rendering Oligobuninae paraphyletic. We further discuss the significance of the relatively small size of this new mustelid as it relates to predictions based on increased aridification of the palaeoclimate and the expansion of open habitats in the Oligocene.

Download Full-text

A new genus and species in the Sporolithales (Corallinophycidae, Rhodophyta) from northern New Zealand: Roseapetra farriae sp. nov.

Phytotaxa ◽

10.11646/phytotaxa.490.1.3 ◽

2021 ◽

Vol 490 (1) ◽

pp. 35-46

Author(s):

WENDY A. NELSON ◽

KATE F. NEILL ◽

BRENTON A. TWIST ◽

JUDY E. SUTHERLAND

Keyword(s):

New Zealand ◽

Intertidal Zone ◽

New Genus ◽

Sequence Data ◽

Phylogenetic Analyses ◽

New Genus And Species ◽

Diagnostic Features ◽

Rocky Reefs ◽

Anatomical Characters

A new genus in the order Sporolithales (Corallinophycidae, Rhodophyta), Roseapetra farriae gen. et sp. nov. is described, based on material from northern New Zealand. Previously placed in the genus Heydrichia on the basis of morpho-anatomical characters, phylogenetic analyses have shown that this northern New Zealand taxon is not resolved with either Heydrichia or Sporolithon, the two genera currently recognized in this order. Roseapetra displays the key diagnostic features of the order, namely the production of cruciately divided tetrasporangia singly within calcified sporangial compartments. In Roseapetra the tetrasporangia are surrounded by an involucre, and mature tetrasporangia are subtended by up to three stalk cells. The tetrasporangial compartments are shed once spores have been released. Gametangial conceptacles are not shed but become buried, with secondary meristem producing lens-like areas of regrowth. While there are several morpho-anatomical characters that separate Roseapetra and Sporolithon, there are none that can be used to distinguish Roseapetra from Heydrichia: recognition of Roseapetra requires sequence data. Roseapetra farriae is found growing in the low intertidal zone on rocky reefs in shaded, understorey habitats.

Download Full-text

A new thylacosmilid (Mammalia, Metatheria, Sparassodonta) from the Miocene of Patagonia, Argentina

Zootaxa ◽

10.11646/zootaxa.2552.1.3 ◽

2010 ◽

Vol 2552 (1) ◽

pp. 55 ◽

Cited By ~ 15

Author(s):

ANALÍA M. FORASIEPI ◽

ALFREDO A. CARLINI

Keyword(s):

Middle Miocene ◽

New Genus ◽

Phylogenetic Analyses ◽

Dental Morphology ◽

New Taxon ◽

Single Specimen ◽

New Genus And Species ◽

The West ◽

Rio Negro ◽

The Family

A new genus and species, Patagosmilus goini, of the family Thylacosmilidae (Mammalia, Metatheria, Sparassodonta) is described here. The new taxon is based on a single specimen collected from the west margin of the Río Chico, in Río Negro Province, Argentina, from the middle Miocene Colloncuran SALMA. Until now, two formally recognized species were encompassed in the family Thylacosmilidae: Thylacosmilus atrox, from the late Miocene-late Pliocene Huayquerian to Chapadmalalan SALMAof Argentina and probably Uruguay; and Anachlysictis gracilis, from the middle Miocene Laventan SALMA of Colombia. Recognition of the Patagonian taxon, Patagosmilus, provides new anatomical data, likely to be included in future phylogenetic analyses. The overall morphology of Patagosmilus suggests that it has a more generalized anatomy than Thylacosmilus. The dental morphology suggests the new Patagonian taxon was probably closer to Thylacosmilus than Anachlysictis. Saber-tooth thylacosmilids have several autapomorphic features in the skull that differentiate them from other sparassodonts, including the delayed replacement or non-replacement of the deciduous last premolar.

Download Full-text

Robiginitomaculum antarcticum gen. nov., sp. nov., a member of the family Hyphomonadaceae, from Antarctic seawater

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY ◽

10.1099/ijs.0.65274-0 ◽

2007 ◽

Vol 57 (11) ◽

pp. 2595-2599 ◽

Cited By ~ 35

Author(s):

Kiyoung Lee ◽

Hong Kum Lee ◽

Tae-Hwan Choi ◽

Jang-Cheon Cho

Keyword(s):

New Genus ◽

Phylogenetic Analyses ◽

Fatty Acid Content ◽

Salinity Range ◽

Rrna Gene ◽

Strictly Aerobic ◽

The Novel ◽

Sequence Comparisons ◽

The Family ◽

The Antarctic

A seawater bacterium, designated IMCC3195T, was isolated from the Antarctic coast. Cells of the novel strain were Gram-negative, rusty-coloured, strictly aerobic, chemoheterotrophic, non-budding and non-motile rods or vibrioids that possessed a thin prostheca. Based on 16S rRNA gene sequence comparisons, the novel strain was most closely related to the genera Hyphomonas (89.4–90.9 %), Maricaulis (90.1–90.4 %), Hirschia (89.0 %) and Oceanicaulis (87.9 %) of the family Hyphomonadaceae. Phylogenetic analyses also showed the Antarctic isolate to be only distantly related to the genera of stalked bacteria of marine origin in the family Hyphomonadaceae. The DNA G+C content of the novel strain was 60.3 mol% and the predominant cellular fatty acids were C18 : 1 ω7c (41.9 %), C17 : 1 ω8c (21.4 %) and C17 : 0 (14.3 %). The major quinone was Q-10. Several phenotypic and chemotaxonomic characteristics, including optimum temperature and salinity range for growth, cell morphology, pigmentation and fatty acid content, differentiated the novel strain from other related genera in the family Hyphomonadaceae. From the taxonomic evidence collected in this study, it is suggested that strain IMCC3195T (=KCCM 42687T=NBRC 103098T) represents a new genus and novel species in the family Hyphomonadaceae, for which the name Robiginitomaculum antarcticum gen. nov., sp. nov. is proposed.

Download Full-text