scholarly journals Candida bromeliacearum sp. nov. and Candida ubatubensis sp. nov., two yeast species isolated from the water tanks of Canistropsis seidelii (Bromeliaceae)

2005 ◽  
Vol 55 (5) ◽  
pp. 2213-2217 ◽  
Author(s):  
Carla C. C. Ruivo ◽  
Marc-André Lachance ◽  
Carlos A. Rosa ◽  
Maurício Bacci ◽  
Fernando C. Pagnocca
Keyword(s):  
Yeast Species ◽  
Large Subunit ◽  
Sequence Divergence ◽  
Small Subunit ◽  
Eastern Brazil ◽  
Small Subunit Rdna ◽  
Large Subunit Rdna ◽  
Ascomycetous Yeasts ◽  
Water Tanks ◽  
Type Strains

Strains belonging to two novel yeast species, Candida bromeliacearum and Candida ubatubensis, were isolated from the bromeliad tank of Canistropsis seidelii (Bromeliaceae) in a sandy coastal plain (restinga) ecosystem site in an Atlantic rainforest of south-eastern Brazil. These species were genetically distinct from all other currently accepted ascomycetous yeasts, based on sequence divergence in the D1/D2 domains of the large-subunit rDNA and in the small-subunit rDNA. The species occupy basal positions in the Metschnikowiaceae clade. The type strains are Candida bromeliacearum UNESP 00-103T (=CBS 10002T=NRRL Y-27811T) and Candida ubatubensis UNESP 01-247RT (=CBS 10003T=NRRL Y-27812T).

scholarly journals Candida heliconiae sp. nov., Candida picinguabensis sp. nov. and Candida saopaulonensis sp. nov., three ascomycetous yeasts from Heliconia velloziana (Heliconiaceae)

2006 ◽  
Vol 56 (5) ◽  
pp. 1147-1151 ◽  
Author(s):  
Carla C. C. Ruivo ◽  
Marc-André Lachance ◽  
Carlos A. Rosa ◽  
Maurício Bacci ◽  
Fernando C. Pagnocca
Keyword(s):  
Forest Ecosystem ◽  
Yeast Species ◽  
Large Subunit ◽  
Sequence Divergence ◽  
Eastern Brazil ◽  
D2 Domain ◽  
Large Subunit Rdna ◽  
Ascomycetous Yeasts ◽  
Year 2000 ◽  
Type Strains

Strains belonging to three novel yeast species, Candida heliconiae (four isolates), Candida picinguabensis (three isolates) and Candida saopaulonensis (two isolates), were recovered in the year 2000 from water of flower bracts of Heliconia velloziana L. Emigd. (Heliconiaceae) found in a forest ecosystem site in an Atlantic rainforest of south-eastern Brazil. C. picinguabensis and C. saopaulonensis were nearly identical in morphology and physiology, but sequence divergence in the D1/D2 domain of the large-subunit rDNA indicated that they should be regarded as different species. They belong to the Metschnikowiaceae clade. C. heliconiae had affinities to Pichia mexicana and related species, but was genetically isolated from all currently accepted species in that group. The type strains are C. heliconiae UNESP 00-91C1T (=CBS 10000T=NRRL Y-27813T), C. picinguabensis UNESP 00-89T (=CBS 9999T=NRRL Y-27814T) and C. saopaulonensis UNESP 00-99T (=CBS 10001T=NRRL Y-27815T).

scholarly journals Blastobotrys americana sp. nov., Blastobotrys illinoisensis sp. nov., Blastobotrys malaysiensis sp. nov., Blastobotrys muscicola sp. nov., Blastobotrys peoriensis sp. nov. and Blastobotrys raffinosifermentans sp. nov., novel anamorphic yeast species

2007 ◽  
Vol 57 (5) ◽  
pp. 1154-1162 ◽  
Author(s):  
Cletus P. Kurtzman
Keyword(s):  
Type Strain ◽  
Yeast Species ◽  
Novel Species ◽  
Large Subunit ◽  
Small Subunit ◽  
Its2 Rdna ◽  
Small Subunit Rdna ◽  
Anamorphic Yeast ◽  
Large Subunit Rdna ◽  
Cytochrome Oxidase Ii

The genus Blastobotrys, which now includes species previously assigned to the synonymous genera Arxula and Sympodiomyces, represents the anamorph of the ascosporogenous genus Trichomonascus. Six novel species are proposed for assignment to Blastobotrys. They were detected from their unique nucleotide sequences in large-subunit rDNA, ITS1–5.8S–ITS2 rDNA, mitochondrial small-subunit rDNA and the cytochrome oxidase II gene. The proposed novel species are Blastobotrys americana sp. nov. (type strain NRRL Y-6844T=CBS 10337T; substrate unknown; Kansas, USA), Blastobotrys illinoisensis sp. nov. (type strain NRRL YB-1343T=CBS 10339T; from forest debris; Illinois, USA), Blastobotrys malaysiensis sp. nov. (type strain NRRL Y-6417T=CBS 10336T; from soil; Malaysia), Blastobotrys muscicola sp. nov. (type strain NRRL Y-7993T=CBS 10338T; from moss; Louisiana, USA), Blastobotrys peoriensis sp. nov. (type strain NRRL YB-2290T=CBS 10340T; from a fungus; Peoria, IL, USA) and Blastobotrys raffinosifermentans sp. nov. (type strain NRRL Y-27150T=CBS 6800T; substrate unknown).

On the phylogeny of Ophiostoma, Ceratocystis s.s., and Microascus, and relationships within Ophiostoma based on partial ribosomal DNA sequences

1993 ◽  
Vol 71 (9) ◽  
pp. 1249-1265 ◽  
Author(s):  
G. Hausner ◽  
J. Reid ◽  
G. R. Klassen
Keyword(s):  
Dna Sequences ◽  
Sequence Data ◽  
Large Subunit ◽  
Small Subunit ◽  
Data Set ◽  
Rdna Sequences ◽  
Morphological Criteria ◽  
Small Subunit Rdna ◽  
Rdna Sequence Data ◽  
Large Subunit Rdna

Phylogenetic analysis of partial rDNA sequences suggests that Ophiostoma should remain the sole genus of the Ophiostomataceae, and this should be the sole family within the Ophiostomatales, whereas Ceratocystis s.s. would be best disposed within the Microascales. Although morphological criteria suggest that the genus Ophiostoma is heterogeneous, analysis of partial small subunit rDNA sequence data shows that Ophiostoma (excluding O. roraimense) represents a monophyletic taxon. Analysis of a partial large subunit rDNA data set, which included sequences from 55 species assignable to Ophiostoma, failed to support the strict subdivision of the genus based on either ascospore characters or the nature of the anamorph. Key words: Ceratocystis, Microascus, Ophiostoma, partial rDNA sequences, phylogeny.

Candida ficus sp. nov., a novel yeast species from the gut of Apriona germari larvae

2012 ◽  
Vol 62 (Pt_11) ◽  
pp. 2805-2809 ◽  
Author(s):  
Feng-Li Hui ◽  
Qiu-Hong Niu ◽  
Tao Ke ◽  
Zheng Liu
Keyword(s):  
Type Strain ◽  
Yeast Species ◽  
Large Subunit ◽  
Sequence Divergence ◽  
Central China ◽  
Rrna Gene ◽  
The Novel ◽  
Ascomycetous Yeasts ◽  
Large Subunit Rrna ◽  
Wood Boring

A novel yeast species is described based on three strains from the gut of wood-boring larvae collected in a tree trunk of Ficus carica cultivated in parks near Nanyang, central China. Phylogenetic analysis based on sequences of the D1/D2 domains of the large subunit rRNA gene showed that these strains occurred in a separate clade that was genetically distinct from all known ascomycetous yeasts. In terms of pairwise sequence divergence, the novel strains differed by 15.3 % divergence from the type strain of Pichia terricola, and by 15.8 % divergence from the type strains of Pichia exigua and Candida rugopelliculosa in the D1/D2 domains. All three are ascomycetous yeasts in the Pichia clade. Unlike P. terricola, P. exigua and C. rugopelliculosa, the novel isolates did not ferment glucose. The name Candida ficus sp. nov. is proposed to accommodate these highly divergent organisms, with STN-8T ( = CICC 1980T = CBS 12638T) as the type strain.

Revisiting the phylogeny of Ocellularieae, the second largest tribe within Graphidaceae (lichenized Ascomycota: Ostropales)

Phytotaxa ◽  
2014 ◽  
Vol 189 (1) ◽  
pp. 52 ◽  
Author(s):  
Ekaphan Kraichak ◽  
Sittiporn Parnmen ◽  
Robert Lücking ◽  
EIMY RIVAS PLATA ◽  
André Aptroot ◽  
...  
Keyword(s):  
Molecular Phylogeny ◽  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Large Subunit ◽  
Small Subunit ◽  
New Combinations ◽  
New Name ◽  
Small Subunit Rdna ◽  
Large Subunit Rdna ◽  
First Time

We present an updated 3-locus molecular phylogeny of tribe Ocellularieae, the second largest tribe within subfamily Graphidoideae in the Graphidaceae. Adding 165 newly generated sequences from the mitochondrial small subunit rDNA (mtSSU), the nuclear large subunit rDNA (nuLSU), and the second largest subunit of the DNA-directed RNA polymerase II (RPB2), we currently distinguish 218 species among the sequenced material, including the outgroup. This corresponds to almost half the species at this point recognized within this tribe. The newly generated sequences include 23 newly described species and one newly described genus published elsewhere in this volume. For the first time, Sarcographina cyclospora Müll. Arg., in spite of its distinctly lirellate ascomata, is shown to belong in tribe Ocellularieae, as strongly supported sister to Ocellularia inturgescens (Müll. Arg.) Mangold. The following six new combinations are proposed: Melanotrema lynceodes (Nyl.) Rivas Plata, Lücking & Lumbsch, Ocellularia curranii (Vain.) Kraichak, Lücking & Lumbsch, O. khasiana (Patw. & Nagarkar) Kraichak, Lücking & Lumbsch, O. cinerea (Müll. Arg.) Kraichak, Lücking & Lumbsch, O. erodens (R. C. Harris) Kraichak, Lücking & Lumbsch, and O. laeviuscula (Nyl) Kraichak, Lücking & Lumbsch. Further, the new name Ocellularia hernandeziana Kraichak, Lücking & Lumbsch is introduced for Myriotrema ecorticatum. The nomenclatural status of the name Ocellularia microstoma is clarified.

Isolation and in vitro cultivation of the aphid pathogenic fungusEntomophthora planchoniana

2001 ◽  
Vol 47 (12) ◽  
pp. 1082-1087 ◽  
Author(s):  
Florian M Freimoser ◽  
Annette B Jensen ◽  
Urs Tuor ◽  
Markus Aebi ◽  
Jørgen Eilenberg
Keyword(s):  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Large Subunit ◽  
Small Subunit ◽  
In Vitro Cultivation ◽  
Small Subunit Rdna ◽  
Fetal Bovine ◽  
Large Subunit Rdna ◽  
First Time

Entomophthora planchoniana is an important fungal pathogen of aphids. Although Entomophthora chromaphidis has been considered a synonym for E. planchoniana, the two species are now separated, and E. planchoniana is reported not to grow in vitro. In this paper, we describe for the first time the isolation and cultivation of this species. Entomophthora planchoniana was isolated from a population of Ovatus crataegarius (Homoptera, Aphididae), which was infected by E. planchoniana only. The isolates did not sporulate, but the sequence of the small subunit rDNA and the restriction fragment length polymorphism patterns of the first part of the large subunit rDNA and the ITS II region confirm that the isolates were E. planchoniana. The isolated fungus grew in a medium consisting of Grace's insect cell culture medium supplemented with lactalbumin hydrolysate, yeastolate, and 10% fetal bovine serum or in GLEN medium with 10% fetal bovine serum. Vegetative cells of E. planchoniana were long and club-shaped and did not stain with Calcofluor, thus suggesting that they were protoplasts.Key words: biological control, entomopathogen, Entomophthorales, protoplast, Zygomycetes.

Novel contributions to the peritrich family Vaginicolidae (Protista: Ciliophora), with morphological and phylogenetic analyses of poorly known species of Pyxicola, Cothurnia and Vaginicola

2019 ◽  
Vol 187 (1) ◽  
pp. 1-30 ◽  
Author(s):  
Borong Lu ◽  
Lifang Li ◽  
Xiaozhong Hu ◽  
Daode Ji ◽  
Khaled A S Al-Rasheid ◽  
...  
Keyword(s):  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Small Subunit ◽  
Its2 Region ◽  
5.8S Rdna ◽  
Small Subunit Rdna ◽  
Distinct Lineage ◽  
Large Subunit Rdna ◽  
First Time

Abstract The classification of loricate peritrich ciliates is difficult because of an accumulation of several taxonomic problems. In the present work, three poorly described vaginicolids, Pyxicola pusilla, Cothurnia ceramicola and Vaginicola tincta, were isolated from the surface of two freshwater/marine algae in China. In our study, the ciliature of Pyxicola and Vaginicola is revealed for the first time, demonstrating the taxonomic value of infundibular polykineties. The small subunit rDNA, ITS1-5.8S rDNA-ITS2 region and large subunit rDNA of the above species were sequenced for the first time. Phylogenetic analyses based on these genes indicated that Pyxicola and Cothurnia are closely related. The present study suggested that the loricate species probably represent a distinct lineage in peritrich evolution and both genera Cothurnia and Thuricola are monophyletic. Pyxicola pusilla, Cothurnia ceramicola and Vaginicola tincta are recircumscribed.

Morphological and molecular characterisation of Xiphinema zagrosense sp. n. (Dorylaimida: Longidoridae) from the Zagros Mountains, Iran

Nematology ◽  
2012 ◽  
Vol 14 (4) ◽  
pp. 445-455 ◽  
Author(s):  
Razieh Ghaemi ◽  
Ebrahim Pourjam ◽  
Majid Pedram ◽  
Robert T. Robbins ◽  
Weimin Ye ◽  
...  
Keyword(s):  
New Species ◽  
Large Subunit ◽  
Close Relation ◽  
Small Subunit ◽  
Zagros Mountains ◽  
Rdna Gene ◽  
Small Subunit Rdna ◽  
Group 6 ◽  
Juvenile Stages ◽  
Large Subunit Rdna

Xiphinema zagrosense sp. n. is described based on morphological and molecular characters. The new species belongs to morphospecies group 6 characterised by having two equally developed female genital branches with spines in uterus and a short, rounded tail. The new species is characterised by having spines in the tubular part of the uterus, body length 3.8-5.0 mm, odontostyle length 151-169 μm, odontophore 94-105 μm, conical, dorsally convex tail, four juvenile stages and absence of males. The new species appears closely related to the members of X. pyrenaicum group characterised by a rounded tail with or without an inconspicuous digitate end and a uterus devoid of Z-differentiation but showing spiniform structures. It is compared with X. aceri, X. adenohystherum, X. iranicum, X. mazandaranense, X. nuragicum, X. pyrenaicum, X. robbinsi, X. sphaerocephalum and X. vuittenezi. In addition to morphological and morphometric data, molecular analyses of the near-full-length small subunit rDNA gene (SSU) and large subunit rDNA gene (LSU) D2/D3 placed the new species in close relation with some species belonging to Xiphinema group 6 but differs from other species in the X. pyrenaicum complex.

scholarly journals Candida aechmeae sp. nov. and Candida vrieseae sp. nov., novel yeast species isolated from the phylloplane of bromeliads in Southern Brazil

2010 ◽  
Vol 60 (1) ◽  
pp. 244-248 ◽  
Author(s):  
Melissa Fontes Landell ◽  
Raisa Billodre ◽  
Jesus P. Ramos ◽  
Orílio Leoncini ◽  
Marilene H. Vainstein ◽  
...  
Keyword(s):  
Type Strain ◽  
Yeast Species ◽  
Novel Species ◽  
Sequence Divergence ◽  
Rrna Gene ◽  
The Novel ◽  
D2 Domain ◽  
Its Gene ◽  
Ascomycetous Yeasts ◽  
Positive Results

Two novel yeast species, Candida aechmeae sp. nov. and Candida vrieseae sp. nov., were isolated from bromeliads in Itapuã Park, Rio Grande do Sul, Brazil. These species are genetically isolated from all other currently recognized ascomycetous yeasts based on their sequence divergence in the D1/D2 domain of the LSU rRNA gene. C. aechmeae sp. nov. is phylogenetically close to Candida ubatubensis, a species also isolated from bromeliads in Brazil, but the novel species can be differentiated on the basis of differences in the D1/D2 domain and positive results for the assimilation of l-arabinose, raffinose, inulin and citrate. Candida vrieseae sp. nov. is phylogenetically placed in a clade near Candida membranifaciens that is composed of several species associated with insects, but the novel species can be differentiated from them by the D1/D2 and ITS gene sequences, positive results for the assimilation of nitrite and a negative result for the assimilation of ethylamine. The type strain for Candida aechmeae sp. nov. is BI153T (=CBS 10831T=NRRL Y-48456T) and the type strain for C. vrieseae sp. nov. is BI146T (=CBS 10829T=NRRL Y-48461T).

scholarly journals The D1/D2 domain of the large-subunit rDNA of the yeast species is unusually polymorphic

2003 ◽  
Vol 4 (3) ◽  
pp. 253-258 ◽  
Author(s):  
M LACHANCE ◽  
H DANIEL ◽  
W MEYER ◽  
G PRASAD ◽  
S GAUTAM ◽  
...  
Keyword(s):  
Yeast Species ◽  
Large Subunit ◽  
D2 Domain ◽  
Large Subunit Rdna
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