scholarly journals Classification of ‘Sarraceniospora aurea’ Furihata et al. 1989 as Actinocorallia aurea sp. nov.

2007 ◽  
Vol 57 (9) ◽  
pp. 2052-2055 ◽  
Author(s):  
Tomohiko Tamura ◽  
Kazunori Hatano ◽  
Ken-ichiro Suzuki
Keyword(s):  
Amino Acids ◽  
Phylogenetic Analysis ◽  
Dna Hybridization ◽  
Type Strain ◽  
Novel Species ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Characteristics

Phylogenetic analysis of ‘Sarraceniospora aurea’ NBRC 14752 and strain NBRC 15120, based on 16S rRNA gene sequences, revealed that these organisms are related to members of the genus Actinocorallia. These organisms contained glutamic acid, alanine and meso-diaminopimelic acid as cell-wall amino acids and the menaquinones MK-9(H4), MK-9(H6) and MK-9(H8). The chemotaxonomic characteristics of the strains were consistent with those of the genus Actinocorallia. However, DNA–DNA hybridization and phenotypic characteristics revealed that the strains differed from the recognized species of the genus Actinocorallia. Therefore, we propose that ‘Sarraceniospora aurea’ NBRC 14752 and strain NBRC 15120 be reclassified in the genus Actinocorallia as a novel species, Actinocorallia aurea sp. nov. (type strain NBRC 14752T=DSM 44434T).

scholarly journals Actinoplanes tropicalis sp. nov. and Actinoplanes cibodasensis sp. nov., isolated from leaf litter

2015 ◽  
Vol 65 (Pt_11) ◽  
pp. 3824-3829 ◽  
Author(s):  
Arif Nurkanto ◽  
Puspita Lisdiyanti ◽  
Moriyuki Hamada ◽  
Shanti Ratnakomala ◽  
Chiyo Shibata ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Leaf Litter ◽  
Dna Hybridization ◽  
Type Strain ◽  
Novel Species ◽  
Botanical Garden ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Characteristics

Two actinomycete strains, designated LIPI11-2-Ac034T and LIPI11-2-Ac042T, were isolated from leaf litter collected from Cibodas Botanical Garden, West Java, Indonesia. Phylogenetic analysis based on 16S rRNA gene sequences suggested that both isolates belong to the genus Actinoplanes. These isolates were closely related to Actinoplanes ferrugineus and Actinoplanes durhamensis with similarity values of 98.2 % and 97.7 % respectively, for strain LIPI11-2-Ac034T, and 99.0 % and 97.4–97.7 % respectively for strain LIPI11-2-Ac042T. Both isolates grew well on ISP 7 medium with brown soluble pigment production. Spores were motile and sporangia were irregular. The isolates contained meso-diaminopimelic acid in cell-wall hydrolysates, and mannose, glucose and galactose in whole-cell hydrolysates. The predominant menaquinone of strain LIPI11-2-Ac034T was MK-9(H4) while that of strain LIPI11-2-Ac042T was MK-9(H6). The major cellular fatty acids were iso-C16 : 0, iso-C15 : 0 and anteiso-C15 : 0 for strain LIPI11-2-Ac034T, and iso-C16 : 0, anteiso-C15 : 0, iso-C15 : 0 and anteiso-C17 : 0 for strain LIPI11-2-Ac042T. Phosphatidylethanolamine was detected as the diagnostic polar lipid. The DNA G+C contents of strains LIPI11-2-Ac034T and LIPI11-2-Ac042T were 71.5 and 70.7 mol%, respectively. Based on the differential phenotypic characteristics and the results of DNA–DNA hybridization and phylogenetic analysis, it is proposed that strains LIPI11-2-Ac034T and LIPI11-2-Ac042T represent two novel species of the genus Actinoplanes, for which the names Actinoplanes tropicalis sp. nov. (type strain LIPI11-2-Ac034T = InaCC A459T = NBRC 110973T) and Actinoplanes cibodasensis sp. nov. (type strain LIPI11-2-Ac042T = InaCC A458T = NBRC 110974T) are proposed.

scholarly journals Paenibacillus glacialis sp. nov., isolated from the Kafni glacier of the Himalayas, India

2010 ◽  
Vol 60 (8) ◽  
pp. 1909-1913 ◽  
Author(s):  
K. Hara Kishore ◽  
Zareena Begum ◽  
Akbar Ali Khan Pathan ◽  
S. Shivaji
Keyword(s):  
Phylogenetic Analysis ◽  
Dna Hybridization ◽  
Type Strain ◽  
Novel Species ◽  
Diaminopimelic Acid ◽  
Phenotypic Traits ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
The Status ◽  
Diamino Acid

A novel strain of the genus Paenibacillus, KFC91T, was isolated from the Kafni glacier of the Himalayas. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain KFC91T clustered with Paenibacillus antarcticus LMG 22078T (98.9 %) and Paenibacillus macquariensis LMG 6935T (98.7 %). The cell wall peptidoglycan contained meso-diaminopimelic acid as the diamino acid, anteiso-C15 : 0 as the predominant fatty acid and MK-7 as the major isoprenoid quinone. Based on the phylogenetic analysis, chemotaxonomic characteristics and other phenotypic traits, strain KFC91T was assigned to the genus Paenibacillus. DNA–DNA hybridization experiments of KFC91T with P. antarcticus and P. macquariensis showed reassociation values of 39 and 52 %, respectively. Thus, it is proposed that strain KFC91T should be assigned the status of a novel species of the genus Paenibacillus and the name proposed is Paenibacillus glacialis sp. nov., with KFC91T (=NCCB 100252T =DSM 22343T) as the type strain.

scholarly journals Nocardiopsis alkaliphila sp. nov., a novel alkaliphilic actinomycete isolated from desert soil in Egypt

2004 ◽  
Vol 54 (1) ◽  
pp. 247-252 ◽  
Author(s):  
Wael N. Hozzein ◽  
Wen-Jun Li ◽  
Mohammed Ibrahim A. Ali ◽  
Ola Hammouda ◽  
Ahmed S. Mousa ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Dna Hybridization ◽  
Type Strain ◽  
Novel Species ◽  
Eastern Desert ◽  
Diaminopimelic Acid ◽  
Phenotypic Characteristics ◽  
Optimum Ph ◽  
Actinomycete Strain ◽  
Rdna Analysis

An alkaliphilic actinomycete strain, designated YIM 80379T, was isolated from a soil sample collected from the eastern desert of Egypt and subjected to polyphasic taxonomy. The strain produced substrate and aerial mycelia on different media, with an optimum pH for growth of 9·5–10 and scarce or no growth at pH 7. Strain YIM 80379T contained meso-diaminopimelic acid, no diagnostic sugars, type PIII phospholipids and MK-10(H6) and MK-10(H8) as the predominant menaquinones. All of these characters assign isolate YIM 80379T consistently to the genus Nocardiopsis. This was confirmed by 16S rDNA analysis. It can be differentiated from all Nocardiopsis species with validly published names by phenotypic characteristics, phylogenetic analysis and DNA–DNA hybridization results. On the basis of polyphasic evidence, a novel species, Nocardiopsis alkaliphila sp. nov., is proposed. The type strain of the species is YIM 80379T (=CCTCC AA001031T=DSM 44657T).

Tistrella bauzanensis sp. nov., isolated from soil

2011 ◽  
Vol 61 (9) ◽  
pp. 2227-2230 ◽  
Author(s):  
De-Chao Zhang ◽  
Hong-Can Liu ◽  
Yu-Guang Zhou ◽  
Franz Schinner ◽  
Rosa Margesin
Keyword(s):  
Phylogenetic Analysis ◽  
Type Strain ◽  
Novel Species ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
16S Rrna Gene Sequences ◽  
Pairwise Similarity ◽  
Phenotypic Characteristics ◽  
Cellular Fatty Acids ◽  
The Family

A Gram-reaction-negative, strictly aerobic, motile, rod-shaped bacterium, designated strain BZ78T, was isolated from soil from an industrial site. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain BZ78T belonged to the family Rhodospirillaceae and formed a coherent cluster with the type strain of Tistrella mobilis (98.3 % pairwise similarity). The predominant cellular fatty acids of strain BZ78T were C18 : 1ω7c (58.3 %), C19 : 0ω8c cyclo (11.5 %), C18 : 1 2-OH (10.9 %) and C14 : 0 3-OH (6.4 %). The predominant ubiquinone was Q-10. The genomic DNA G+C content of strain BZ78T was 65.8 mol%. On the basis of phenotypic characteristics, phylogenetic analysis and DNA–DNA relatedness data, strain BZ78T is considered to represent a novel species of the genus Tistrella, for which the name Tistrella bauzanensis sp. nov. is proposed. The type strain is BZ78T ( = DSM 22817T  = CGMCC 1.10188T  = LMG 26047T).

Dyadobacter koreensis sp. nov., isolated from fresh water

2007 ◽  
Vol 57 (6) ◽  
pp. 1227-1231 ◽  
Author(s):  
Keun Sik Baik ◽  
Mi Sun Kim ◽  
Eun Mi Kim ◽  
Hyung Rak Kim ◽  
Chi Nam Seong
Keyword(s):  
Dna Hybridization ◽  
Type Strain ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Characteristics ◽  
Gram Negative ◽  
Light Yellow ◽  
Relatedness Data

A non-motile, rod-shaped, light-yellow-pigmented bacterium, designated strain WPCB159T, was isolated from freshwater samples collected from the Woopo wetland in Korea. The cells were Gram-negative, aerobic and catalase- and oxidase-positive. The major fatty acids were C16 : 1 ω7c (34.8 %), iso-C15 : 0 (24.2 %) and C16 : 0 (9.4 %). The DNA G+C content was 44 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain WPCB159T forms a lineage within the genus Dyadobacter (family ‘Flexibacteraceae’) and is closely related to Dyadobacter hamtensis HHS 11T (97.8 % sequence similarity) and to other members of the genus Dyadobacter (95.2–96.8 % sequence similarity). The phenotypic characteristics and DNA–DNA hybridization relatedness data indicate that strain WPCB159T should be distinguished from D. hamtensis HHS 11T. On the basis of the evidence presented in this study, strain WPCB159T represents a novel species of the genus Dyadobacter, for which the name Dyadobacter koreensis sp. nov. is proposed. The type strain is WPCB159T (=KCTC 12537T=NBRC 101116T).

scholarly journals Cohnella xylanilytica sp. nov. and Cohnella terrae sp. nov., xylanolytic bacteria from soil

2010 ◽  
Vol 60 (12) ◽  
pp. 2913-2917 ◽  
Author(s):  
Saowapar Khianngam ◽  
Somboon Tanasupawat ◽  
Ancharida Akaracharanya ◽  
Kwang Kyu Kim ◽  
Keun Chul Lee ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Type Strain ◽  
Novel Species ◽  
Sequence Similarity ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Physiological And Biochemical Characteristics ◽  
Degrading Bacteria ◽  
Physiological And Biochemical

Two xylan-degrading bacteria, strains MX15-2T and MX21-2T, were isolated from soils collected in Nan province, Thailand. Cells were Gram-reaction-positive, facultatively anaerobic, spore-forming and rod-shaped. They contained meso-diaminopimelic acid in the cell-wall peptidoglycan. The major menaquinone was MK-7. iso-C16 : 0 and anteiso-C15 : 0 were the predominant cellular fatty acids. Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and lysyl-phosphatidylglycerol were the major polar lipids. The genomic DNA G+C contents of strains MX15-2T and MX21-2T were 63.0 and 65.1 mol%, respectively. Phylogenetic analysis using 16S rRNA gene sequences showed that strains MX15-2T and MX21-2T were affiliated with the genus Cohnella and were closely related to Cohnella thermotolerans CCUG 47242T, with 96.5 and 95.6 % sequence similarity, respectively. The strains could be clearly distinguished from each other and from all known species of the genus Cohnella based on their physiological and biochemical characteristics as well as their phylogenetic positions and levels of DNA–DNA hybridization. Therefore, these two strains represent novel species of the genus Cohnella, for which the names Cohnella xylanilytica sp. nov. (type strain MX15-2T =KCTC 22294T =PCU 309T =TISTR 1891T) and Cohnella terrae sp. nov. (type strain MX21-2T =KCTC 22295T =PCU 310T =TISTR 1892T) are proposed.

scholarly journals Paenibacillus yunnanensis sp. nov., isolated from Pu'er tea

2015 ◽  
Vol 65 (Pt_11) ◽  
pp. 3806-3811 ◽  
Author(s):  
Lili Niu ◽  
Tianyi Tang ◽  
Zhongliang Ma ◽  
Lei Song ◽  
Kegui Zhang ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
16S Rrna ◽  
Dna Hybridization ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Characteristics ◽  
Hybridization Data ◽  
Gram Staining ◽  
Diamino Acid

A novel Gram-staining-positive, aerobic, endospore-forming, rod-shaped bacterial strain, YN2T, was isolated from ripened Pu'er tea. Phylogenetic analysis of 16S rRNA gene sequences showed that the strain represented a novel species of the genus Paenibacillus. The strains most closely related to strain YN2T were Paenibacillus vulneris JCM 18268T and Paenibacillus rigui JCM 16352T, with 16S rRNA similarities of 98.6 and 95.5 %, respectively. Chemotaxonomic data supported the affiliation of the new isolate to the genus Paenibacillus, including MK-7 as the major menaquinone, DNA G+C content of 51 mol%, cell-wall type A1γ (meso-diaminopimelic acid as the diagnostic diamino acid) and anteiso-C15 : 0 and iso-C16 : 0 as the major fatty acids. Major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylmonomethylethanolamine and phospholipid. Strain YN2T could be differentiated from recognized species of the genus Paenibacillus based on phenotypic characteristics, chemotaxonomic differences, phylogenetic analysis and DNA–DNA hybridization data. On the basis of evidence from this polyphasic study, Paenibacillus yunnanensis sp. nov., is proposed, with strain YN2T ( = CGMCC 1.12968T = JCM 30953T) as the type strain.

scholarly journals Sporosarcina koreensis sp. nov. and Sporosarcina soli sp. nov., isolated from soil in Korea

2007 ◽  
Vol 57 (8) ◽  
pp. 1694-1698 ◽  
Author(s):  
Soon-Wo Kwon ◽  
Byung-Yong Kim ◽  
Jaekyeong Song ◽  
Hang-Yeon Weon ◽  
Peter Schumann ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Type Strain ◽  
Novel Species ◽  
Sequence Similarity ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Upland Soil ◽  
Cellular Fatty Acids ◽  
Type Strains

Two Gram-positive, aerobic, spore-forming rods, F73T and I80T, were isolated from upland soil. A phylogenetic analysis of 16S rRNA gene sequences placed both isolates within the genus Sporosarcina, and showed a sequence similarity of 98.9 % between the two strains and a similarity of approximately 94.6–97.3 % with respect to Sporosarcina species with validly published names. The values for DNA–DNA relatedness between the two isolates and related type strains of the genus Sporosarcina were below 28.0 %. For both strains, the major cellular fatty acids were anteiso-C15 : 0 and iso-C15 : 0. In both cases, the cell-wall peptidoglycan was of the A4α type (l-Lys–d-Glu) and the major menaquinone was MK-7. Diaminopimelic acid was absent from both strains. The genomic DNA G+C contents of strains F73T and I80T were 46.5 and 44.5 mol%, respectively. On the basis of the phylogenetic analysis and physiological and chemotaxonomic data, the isolates represent two novel species of the genus Sporosarcina, for which the names Sporosarcina koreensis sp. nov. (type strain F73T =KACC 11299T =DSM 16921T) and Sporosarcina soli sp. nov. (type strain I80T =KACC 11300T =DSM 16920T) are proposed.

scholarly journals Corynebacterium hansenii sp. nov., an α-glucosidase-negative bacterium related to Corynebacterium xerosis

2007 ◽  
Vol 57 (5) ◽  
pp. 1113-1116 ◽  
Author(s):  
François N. R. Renaud ◽  
Alain Le Coustumier ◽  
Nathalie Wilhem ◽  
Dominique Aubel ◽  
Philippe Riegel ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Type Strain ◽  
Novel Species ◽  
Carbon Assimilation ◽  
Rrna Gene ◽  
Negative Bacterium ◽  
16S Rrna Gene Sequences ◽  
Glucose Fermentation ◽  
Biochemical Characteristics ◽  
Hybridization Data

A novel strain, C-138T, belonging to the genus Corynebacterium was isolated from a severe thigh liposarcoma infection and its differentiation from Corynebacterium xerosis and Corynebacterium freneyi is described. Analysis of 16S rRNA gene sequences, rpoB sequences and the PCR profile of the 16S–23S spacer regions was not conclusive enough to differentiate strain C-138T from C. xerosis and C. freneyi. However, according to DNA–DNA hybridization data, strain C-138T constitutes a member of a distinct novel species. It can be differentiated from strains of C. xerosis and C. freneyi by colony morphology, the absence of α-glucosidase and some biochemical characteristics such as glucose fermentation at 42 °C and carbon assimilation substrates. The name Corynebacterium hansenii sp. nov. is proposed for this novel species; the type strain is C-138T (=CIP 108444T=CCUG 53252T).

scholarly journals Description of Sphingobium fuliginis sp. nov., a phenanthrene-degrading bacterium from a fly ash dumping site, and reclassification of Sphingomonas cloacae as Sphingobium cloacae comb. nov.

2006 ◽  
Vol 56 (9) ◽  
pp. 2147-2152 ◽  
Author(s):  
Om Prakash ◽  
Rup Lal
Keyword(s):  
Phylogenetic Analysis ◽  
Fatty Acid ◽  
Fly Ash ◽  
16S Rrna ◽  
Dna Hybridization ◽  
Type Strain ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Dumping Site ◽  
Degrading Bacterium

A phenanthrene-degrading bacterium, strain TKPT, was isolated from a fly ash dumping site of the thermal power plant in Panki, Kanpur, India, by an enrichment culture method using phenanthrene as the sole source of carbon and energy. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain belonged to the genus Sphingobium, as it showed highest sequence similarity to Sphingobium herbicidovorans DSM 11019T (97.3 %) and Sphingomonas cloacae JCM 10874T (96.5 %), compared with only 91–93 % similarity to members of other genera such as Sphingomonas sensu stricto, Novosphingobium, Sphingopyxis and Sphingosinicella. In DNA–DNA hybridization experiments with strains that were closely related phylogenetically and in terms of 16S rRNA gene sequences, i.e. Sphingobium herbicidovorans DSM 11019T and Sphingomonas cloacae JCM 10874T, strain TKPT showed less than 70 % relatedness. Strain TKPT contained sphingoglycolipids SGL-1 and SGL-2 and 18 : 1ω7c as the predominant fatty acid, with 16 : 0 as a minor component and 14 : 0 2-OH as the major 2-hydroxy fatty acid. Thus, phylogenetic analysis, DNA–DNA hybridization, fatty acid and polar lipid profiles and differences in physiological and morphological features from the most closely related members of the Sphingobium group showed that strain TKPT represents a distinct species of Sphingobium. The name Sphingobium fuliginis sp. nov. is proposed, with the type strain TKPT (=MTCC 7295T=CCM 7327T). Sphingomonas cloacae JCM 10874T formed a coherent cluster with members of Sphingobium, did not reduce nitrate to nitrite and had a fatty acid profile similar to those of Sphingobium species; hence Sphingomonas cloacae should be transferred to the genus Sphingobium as Sphingobium cloacae comb. nov., with the type strain JCM 10874T (=DSM 14926T).

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