scholarly journals Description of Sphingosinicella xenopeptidilytica sp. nov., a β-peptide-degrading species, and emended descriptions of the genus Sphingosinicella and the species Sphingosinicella microcystinivorans

2007 ◽  
Vol 57 (1) ◽  
pp. 107-113 ◽  
Author(s):  
Birgit Geueke ◽  
Hans-Jürgen Busse ◽  
Thomas Fleischmann ◽  
Peter Kämpfer ◽  
Hans-Peter E. Kohler
Keyword(s):  
Dna Hybridization ◽  
Gene Sequence ◽  
Treatment Plant ◽  
Pcr Analysis ◽  
Aminopeptidase Activity ◽  
Aeration Tank ◽  
Rrna Gene ◽  
Gene Sequence Analysis ◽  
Bacterium Strain ◽  
Type Strains

A Gram-negative, rod-shaped bacterium, strain 3-2W4T, was isolated from the aeration tank of a wastewater treatment plant in Zurich and was found to have the exceptional capacity to degrade synthetic β-peptides. 16S rRNA gene sequence analysis showed that strain 3-2W4T is closely related to Sphingosinicella microcystinivorans Y2T, but DNA–DNA hybridization experiments between these two strains revealed that they belong to two different species. The two strains displayed different fingerprints after PCR analysis using the repetitive primers BOX, ERIC and REP. Strain 3-2W4T did not degrade microcystin, which is a characteristic trait of Sphingosinicella microcystinivorans Y2T. Like Sphingosinicella microcystinivorans Y2T, strain 3-2W4T had the following characteristics: fatty acids comprising mainly C18 : 1 ω7c, summed feature 3 (C16 : 1 ω7c and/or iso-C15 : 0 2-OH) and C16 : 0, the presence of ubiquinone Q-10 and sym-homospermidine as the predominant polyamine compound. The polar lipid profiles of the two strains were almost identical, consisting of phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol and sphingoglycolipid. Strain 3-2W4T and Sphingosinicella microcystinivorans Y2T utilized the β-peptides H-βhVal-βhAla-βhLeu-OH and H-βhAla-βhLeu-OH as sole carbon and energy sources and shared β-peptidyl aminopeptidase activity in common, which distinguishes them from Sphingomonas and Sphingopyxis type strains. On the basis of these results, strain 3-2W4T represents a novel species of the genus Sphingosinicella, for which the name Sphingosinicella xenopeptidilytica sp. nov. is proposed. The type strain is 3-2W4T (=DSM 17130T=CCUG 52537T). The descriptions of the genus Sphingosinicella and the species Sphingosinicella microcystinivorans are emended.

scholarly journals Novosphingobium gossypii sp. nov., isolated from Gossypium hirsutum

2015 ◽  
Vol 65 (Pt_9) ◽  
pp. 2831-2837 ◽  
Author(s):  
Peter Kämpfer ◽  
Karin Martin ◽  
John A. McInroy ◽  
Stefanie P. Glaeser
Keyword(s):  
Gossypium Hirsutum ◽  
Gene Sequence ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
High Sequence Similarity ◽  
Polar Lipid Profile ◽  
Gene Sequence Analysis ◽  
Bacterium Strain ◽  
Type Strains ◽  
Sequence Similarities

A Gram-stain-negative, rod-shaped, non-spore-forming bacterium (strain JM-1396T) producing a yellow pigment, was isolated from the healthy internal stem tissue of post-harvest cotton (Gossypium hirsutum, cultivar ‘DES-119’) grown at the Plant Breeding Unit at the E. V. Smith Research Center in Tallassee (Macon county), AL, USA. 16S rRNA gene sequence analysis of strain JM-1396T showed high sequence similarity values to the type strains of Novosphingobium mathurense, Novosphingobium panipatense (both 98.6 %) and Novosphingobium barchaimii (98.5 %); sequence similarities to all other type strains of species of the genus Novosphingobium were below 98.3 %. DNA–DNA pairing experiments of the DNA of strain JM-1396T and N. mathurense SM117T, N. panipatense SM16T and N. barchaimii DSM 25411T showed low relatedness values of 8 % (reciprocal 7 %), 24 % (reciprocal 26 %) and 19 % (reciprocal 25 %), respectively. Ubiquinone Q-10 was detected as the dominant quinone; the fatty acids C18 : 1ω7c (71.0 %) and the typical 2-hydroxy fatty acid, C14 : 0 2-OH (11.7 %), were detected as typical components. The polar lipid profile contained the diagnostic lipids diphosphatidylglycerol, phosphatidylethanolamine, sphingoglycolipid and phosphatidylcholine. The polyamine pattern contained the major compound spermidine and only minor amounts of other polyamines. All these data revealed that strain JM-1396T represents a novel species of the genus Novosphingobium. For this reason we propose the name Novosphingobium gossypii sp. nov. with the type strain JM-1396T ( = LMG 28605T = CCM 8569T = CIP 110884T).

Terrabacter carboxydivorans sp. nov., a carbon monoxide-oxidizing actinomycete

2011 ◽  
Vol 61 (3) ◽  
pp. 482-486 ◽  
Author(s):  
Sung M. Kim ◽  
Sae W. Park ◽  
Sang T. Park ◽  
Young M. Kim
Keyword(s):  
Carbon Monoxide ◽  
Dna Hybridization ◽  
Gene Sequence ◽  
Phylogenetic Analyses ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Polar Lipid Profile ◽  
Gene Sequence Analysis ◽  
Diamino Acid ◽  
Type Strains

A bacterial strain, PY2T, capable of oxidizing carbon monoxide, was isolated from a soil sample collected from a roadside at Yonsei University, Seoul, Korea. On the basis of 16S rRNA gene sequence analysis, strain PY2T was shown to belong to the genus Terrabacter and was most closely related to Terrabacter lapilli LR-26T (99.1 % similarity). Strain PY2T was characterized chemotaxonomically as having iso-C15 : 0 as the predominant fatty acid, MK-8(H4) as the major menaquinone, ll-diaminopimelic acid as the diagnostic diamino acid of the cell wall, as possessing a polar lipid profile that included diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and unknown amino-containing phosphoglycolipids, and having a DNA G+C content of 75.6 mol%. DNA–DNA relatedness values between strain PY2T and the type strains of T. lapilli, Terrabacter tumescens, Terrabacter terrae and Terrabacter aerolatus were 20.0 %, 22.9 %, 35.9 % and 64.5 %, respectively. Based on the combined evidence from the phylogenetic analyses, chemotaxonomic data and DNA–DNA hybridization experiments, it is proposed that strain PY2T represents a novel species for which the name Terrabacter carboxydivorans sp. nov. is proposed. The type strain is PY2T (=KCCM 42922T=JCM 16259T).

Blastochloris gulmargensis sp. nov., isolated from an epilithic phototrophic biofilm

2011 ◽  
Vol 61 (8) ◽  
pp. 1811-1816 ◽  
Author(s):  
V. Venkata Ramana ◽  
Shivali Kapoor ◽  
E. Shobha ◽  
E. V. V. Ramprasad ◽  
Ch. Sasikala ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Dna Hybridization ◽  
Gene Sequence ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Phototrophic Biofilm ◽  
Gene Sequence Analysis ◽  
Bacterium Strain ◽  
Blastochloris Viridis

A novel Gram-negative, motile, bacteriochlorophyll b-containing purple non-sulfur bacterium, strain JA248T, was isolated from phototrophic enrichments of a yellow–green epilithic biofilm sample collected from Gulmarg, India. The genomic DNA G+C content of strain JA248T was 63.8 mol%. A phylogenetic tree based on 16S rRNA gene sequence analysis showed that strain JA248T had highest similarity to members of the genus Blastochloris and was closely related to Blastochloris sulfoviridis DSM 729T (98.5 % sequence similarity) and Blastochloris viridis DSM 133T (98.4 %) of the class Alphaproteobacteria. Strain JA248T was characterized based on polyphasic taxonomy, and distinct phenotypic and molecular differences based on DNA–DNA hybridization (relatedness of <46.5 % with the two species of the genus Blastochloris), multilocus sequence analysis, and phenotypic and chemotaxonomic evidence separated strain JA248T from other species of the genus Blastochloris. Strain JA248T therefore represents a novel species in the genus Blastochloris, for which the name Blastochloris gulmargensis sp. nov. is proposed. The type strain is JA248T ( = JCM 14795T  = DSM 19786T).

scholarly journals Paenibacillus chungangensis sp. nov., isolated from a tidal-flat sediment

2011 ◽  
Vol 61 (2) ◽  
pp. 281-285 ◽  
Author(s):  
Mi-Hak Park ◽  
Jitsopin Traiwan ◽  
Min Young Jung ◽  
Yun Sung Nam ◽  
Ji Hoon Jeong ◽  
...  
Keyword(s):  
Tidal Flat ◽  
Gene Sequence ◽  
Sequence Similarity ◽  
Cellular Fatty Acid ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Tidal Flat Sediment ◽  
Gene Sequence Analysis ◽  
Bacterium Strain ◽  
Type Strains

A Gram-stain-positive, rod-shaped, endospore-forming bacterium, strain CAU 9038T, was isolated from a tidal-flat sediment of DaeYiJac Island, Republic of Korea, and its taxonomic position was investigated using a polyphasic approach. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol, the major isoprenoid quinone was MK-7 and the dominant cellular fatty acid was anteiso-C15 : 0. The DNA G+C content was 51.6 mol%. 16S rRNA gene sequence analysis showed that the strain belonged to the genus Paenibacillus, with <96.1 % sequence similarity to type strains of Paenibacillus species with validly published names. The most closely related type strains to CAU 9038T were Paenibacillus thailandensis S3-4AT (96.1 % similarity) and Paenibacillus agaridevorans DSM 1355T (95.3 %). The phenotypic, chemotaxonomic and genotypic data clearly indicated that strain CAU 9038T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus chungangensis sp. nov. is proposed. The type strain is CAU 9038T (=KCTC 13717T =CCUG 59129T).

scholarly journals Proposal of Lentzea deserti (Okoro et al. 2010) Nouioui et al. 2018 as a later heterotypic synonym of Lentzea atacamensis (Okoro et al. 2010) Nouioui et al. 2018 and an emended description of Lentzea atacamensis

PLoS ONE ◽  
2021 ◽  
Vol 16 (2) ◽  
pp. e0246533
Author(s):  
Mo Ping ◽  
Zhao Yun-Lin ◽  
Liu Jun ◽  
Gao Jian ◽  
Xu Zheng-Gang
Keyword(s):  
Dna Hybridization ◽  
Gene Sequence ◽  
Sequence Similarity ◽  
Bacterial Species ◽  
Rrna Gene ◽  
Gene Sequence Analysis ◽  
Emended Description ◽  
Relationship Of ◽  
Type Strains ◽  
The 16S Rrna Gene

The taxonomic relationship of Lentzea atacamensis and Lentzea deserti were re-evaluated using comparative genome analysis. The 16S rRNA gene sequence analysis indicated that the type strains of L. atacamensis and L. deserti shared 99.7% sequence similarity. The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between the genomes of two type strains were 88.6% and 98.8%, respectively, greater than the two recognized thresholds values of 70% dDDH and 95–96% ANI for bacterial species delineation. These results suggested that L. atacamensis and L. deserti should share the same taxonomic position. And this conclusion was further supported by similar phenotypic and chemotaxonomic features between them. Therefore, we propose that L. deserti is a later heterotypic synonym of L. atacamensis.

scholarly journals Brachybacterium zhongshanense sp. nov., a cellulose-decomposing bacterium from sediment along the Qijiang River, Zhongshan City, China

2007 ◽  
Vol 57 (11) ◽  
pp. 2519-2524 ◽  
Author(s):  
Guoxia Zhang ◽  
Guoqu Zeng ◽  
Xiaowei Cai ◽  
Suier Deng ◽  
Huidong Luo ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Insertion Sequence ◽  
Gene Sequence ◽  
Rrna Gene ◽  
Ratio Analysis ◽  
Polyphasic Analysis ◽  
Hybridization Data ◽  
Dna Base ◽  
Gene Sequence Analysis ◽  
Bacterium Strain

A cellulose-decomposing bacterium, strain JBT, was isolated from sediments along the Qijiang River, Zhongshan City, China. Results of morphological, biochemical and chemotaxonomic characterization and 16S rRNA gene sequence analysis revealed that strain JBT belonged to the genus Brachybacterium. Insertion sequence-PCR fingerprinting patterns, DNA base ratio analysis and DNA–DNA hybridization data showed that strain JBT differed from recognized species of the genus Brachybacterium. Based on polyphasic analysis, strain JBT represents a novel species of the genus Brachybacterium, for which the name Brachybacterium zhongshanense sp. nov. is proposed. The type strain is JBT (=LMG 23926T=CGMCC 1.6508T=DSM 18832T).

scholarly journals Chromohalobacter sarecensis sp. nov., a psychrotolerant moderate halophile isolated from the saline Andean region of Bolivia

2004 ◽  
Vol 54 (6) ◽  
pp. 1921-1926 ◽  
Author(s):  
Jorge Quillaguamán ◽  
Osvaldo Delgado ◽  
Bo Mattiasson ◽  
Rajni Hatti-Kaul
Keyword(s):  
Gene Sequence ◽  
Rrna Gene ◽  
Moderate Halophile ◽  
Bolivian Andes ◽  
Gene Sequence Analysis ◽  
Nutritional Characteristics ◽  
Laguna Verde ◽  
Bacterium Strain ◽  
Ph Range ◽  
Type Strains

A moderately halophilic, aerobic, motile, Gram-negative, rod-shaped bacterium (strain LV4T) was isolated from saline soil around the lake Laguna Verde in the Bolivian Andes. The organism is a heterotroph, able to utilize various carbohydrates as a carbon source. It showed tryptophan deaminase, oxidase and catalase activity, but was unable to produce indole or H2S; nitrate was not reduced. The G+C content of the genomic DNA was 56·1 mol%. The pH range for growth was 5–10, temperature range was 0–45 °C and the range of NaCl concentrations was 0–25 % (w/v). On the basis of 16S rRNA gene sequence analysis, strain LV4T was found to be closely related to Chromohalobacter canadensis DSM 6769T and Pseudomonas beijerinckii DSM 7218T; however, its DNA–DNA relatedness with these type strains was low. Strain LV4T resembled other Chromohalobacter species with respect to various physiological, biochemical and nutritional characteristics but also exhibited differences. Thus, a novel species, Chromohalobacter sarecensis sp. nov., is proposed, with LV4T (=CCUG 47987T=ATCC BAA-761T) as the type strain.

scholarly journals Thermomonas koreensis sp. nov., a mesophilic bacterium isolated from a ginseng field

2006 ◽  
Vol 56 (7) ◽  
pp. 1615-1619 ◽  
Author(s):  
Myung Kyum Kim ◽  
Wan-Taek Im ◽  
Jun-Gyo In ◽  
Sung-Hoon Kim ◽  
Deok-Chun Yang
Keyword(s):  
Dna Hybridization ◽  
Gene Sequence ◽  
Sequence Similarity ◽  
Distinct Species ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
Gene Sequence Analysis ◽  
Mesophilic Bacterium ◽  
Bacterium Strain ◽  
Physiological And Biochemical

A Gram-negative, non-spore-forming, rod-shaped, motile bacterium, strain Ko06T, was isolated from soil from a ginseng field in South Korea and was characterized in order to determine its taxonomic position. 16S rRNA gene sequence analysis revealed that strain Ko06T belongs to the Gammaproteobacteria, and the highest levels of sequence similarity were with Thermomonas brevis LMG 21746T (98.4 %), Thermomonas fusca LMG 21737T (97.7 %), Thermomonas haemolytica A50-7-3T (96.5 %) and Thermomonas hydrothermalis SGM-6T (95.8 %). Chemotaxonomic data revealed that strain Ko06T possesses ubiquinone Q-8 and that the predominant fatty acids are C15 : 0 iso, C11 : 0 iso and C11 : 0 iso 3-OH, all of which corroborated assignment of the strain to the genus Thermomonas. The results of DNA–DNA hybridization and physiological and biochemical tests clearly demonstrated that strain Ko06T represents a distinct species. On the basis of these data, strain Ko06T (=KCTC 12540T=NBRC 101155T) should be classified as the type strain of a novel Thermomonas species, for which the name Thermomonas koreensis sp. nov. is proposed.

Williamsia sterculiae sp. nov., isolated from a Chinese medicinal plant

2013 ◽  
Vol 63 (Pt_11) ◽  
pp. 4158-4162 ◽  
Author(s):  
Xiao-Mei Fang ◽  
Jing Su ◽  
Hao Wang ◽  
Yu-Zhen Wei ◽  
Tao Zhang ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Gene Sequence ◽  
Novel Species ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Content Type ◽  
Link Type ◽  
Gene Sequence Analysis ◽  
Type Strains ◽  
Chinese Medicinal Plant

Two actinobacterial strains, CPCC 203464T and CPCC 203448, isolated from surface-sterilized stems of medicinal plants were subjected to a polyphasic taxonomic study. These two aerobic organisms formed pale yellow colonies on tryptic soy agar (TSA). Cells were Gram-stain-positive, non-acid-fast, non-motile, rod- or coccoid-like elements. Comparative 16S rRNA gene sequence analysis indicated that strains CPCC 203464T and CPCC 203448 were most closely related to the type strains of the species of the genus Williamsia . Chemotaxonomic properties such as containing meso-diaminopimelic acid in the cell wall, arabinose, galactose and ribose being the whole-cell hydrolysate sugars, phosphatidylethanolamine (PE), diphosphatidylglycerol (DPG), phosphatidylglycerol (PG) and phosphatidylinositol (PI) as the phospholipids, and C16 : 0, 10-methyl C18 : 0, C18 : 1ω9c, C16 : 1ω7c and/or iso-C15 : 0 2-OH as major fatty acids supported the affiliation of strains CPCC 203464T and CPCC 203448 to the genus Williamsia . The DNA–DNA hybridization values in combination with differentiating chemotaxonomic and physiological characteristics strongly suggested that these two isolates should be classified as representatives of a novel species of the genus Williamsia . The name Williamsia sterculiae sp. nov. is proposed, with strain CPCC 203464T ( = DSM 45741T = KCTC 29118T) as the type strain.

scholarly journals Variovorax soli sp. nov., isolated from greenhouse soil

2006 ◽  
Vol 56 (12) ◽  
pp. 2899-2901 ◽  
Author(s):  
Byung-Yong Kim ◽  
Hang-Yeon Weon ◽  
Seung-Hee Yoo ◽  
Seon-Young Lee ◽  
Soon-Wo Kwon ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Gene Sequence ◽  
Novel Species ◽  
Rrna Gene ◽  
The Novel ◽  
Greenhouse Soil ◽  
Variovorax Paradoxus ◽  
Gene Sequence Analysis ◽  
Bacterium Strain ◽  
Sequence Similarities

A Gram-negative, rod-shaped, non-spore-forming bacterium, strain GH9-3T, isolated from greenhouse soil, was investigated in a polyphasic study. The novel organism grew at 10–35 °C, 0–3 % NaCl and pH 5–9. It had ubiquinone 8 (Q-8) as the predominant isoprenoid quinone and possessed C16 : 0, summed feature 3, C17 : 0 cyclo and C18 : 1 ω7c as the major fatty acids (together representing 87.4 % of the total). The DNA G+C content was 67.1 mol%. 16S rRNA gene sequence analysis of strain GH9-3T showed that it grouped within the Variovorax cluster, with highest sequence similarities to Variovorax paradoxus IAM 12373T (98.3 %) and Variovorax dokdonensis DS-43T (98.0 %). DNA–DNA hybridization values between strain GH9-3T and V. paradoxus DSM 30034T and V. dokdonensis DS-43T were 38 and 29 %, respectively. Based on phenotypic, chemotaxonomic and phylogenetic features, it is proposed that strain GH9-3T represents a novel species of the genus Variovorax with the name Variovorax soli sp. nov. The type strain is GH9-3T (=KACC 11579T=DSM 18216T).

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