scholarly journals Wenxinia marina gen. nov., sp. nov., a novel member of the Roseobacter clade isolated from oilfield sediments of the South China Sea

2007 ◽  
Vol 57 (8) ◽  
pp. 1711-1716 ◽  
Author(s):  
Jiao-Yan Ying ◽  
Bao-Jun Wang ◽  
Xin Dai ◽  
Su-Sheng Yang ◽  
Shuang-Jiang Liu ◽  
...  
Keyword(s):  
South China Sea ◽  
South China ◽  
The South China Sea ◽  
Rrna Gene ◽  
The South ◽  
China Sea ◽  
The Family ◽  
Dinoroseobacter Shibae ◽  
Novel Genus And Species ◽  
Sequence Similarities

An aerobic and heterotrophic, Gram-negative bacterial isolate, strain HY34T, was isolated from sediment of an oilfield in the South China Sea, China. The taxonomy of strain HY34T was studied by phenotypic and phylogenetic methods. Strain HY34T formed faint-pink colonies on marine agar 2216. Cells of strain HY34T were non-motile, ovoid or short rods. Strain HY34T was positive for catalase and oxidase, and nitrate was reduced to nitrite. The nearly complete 16S rRNA gene sequence of strain HY34T was obtained and sequence analysis showed that it, together with the genus Rubellimicrobium, formed a distinct clade close to some members of the Roseobacter clade in the family Rhodobacteraceae, and it showed highest sequence similarities to Oceanicola granulosus HTCC2516T (93.8 %), Silicibacter lacuscaerulensis ITI-1157T (93.3 %), Dinoroseobacter shibae DFL 12T (93.3 %) and Rubellimicrobium thermophilum C-lvk-R2A-2T (92.2 %). Bacteriochlorophyll a was not detected. The ubiquinone system was Q-10. The major polar lipids were phosphatidylglycerol, phosphatidylcholine and an unidentified glycolipid. The major fatty acids (>10 %) were C18 : 1 ω7c and C16 : 0. The DNA G+C content of this strain was 69.4 mol%. A polyphasic analysis supported the conclusion that this strain represents a novel genus and species, which we designated Wenxinia marina gen. nov., sp. nov. The type strain of Wenxinia marina is HY34T (=CGMCC 1.6105T =JCM 14017T).

Erythrobacter nanhaisediminis sp. nov., isolated from marine sediment of the South China Sea

2010 ◽  
Vol 60 (9) ◽  
pp. 2215-2220 ◽  
Author(s):  
Mingshuang Xu ◽  
Yuhua Xin ◽  
Yong Yu ◽  
Jianli Zhang ◽  
Yuguang Zhou ◽  
...  
Keyword(s):  
South China Sea ◽  
South China ◽  
Gene Sequence ◽  
The South China Sea ◽  
Rrna Gene ◽  
The South ◽  
China Sea ◽  
Bacterium Strain ◽  
Ubiquinone 10 ◽  
Sequence Similarities

A novel Gram-negative, orange-pigmented, slightly halophilic, rod-shaped bacterium, strain T30T, was isolated from sediment from the South China Sea. Phylogenetic analysis showed that strain T30T was a member of the genus Erythrobacter, sharing highest 16S rRNA gene sequence similarities with Erythrobacter aquimaris JCM 12189T (99.5 %) and Erythrobacter vulgaris DSM 17792T (99.0 %). Levels of DNA–DNA relatedness between strain T30T and closely related strains of Erythrobacter species ranged from 14.5 to 56.9 %.The isolate lacked bacteriochlorophyll a and contained ubiquinone-10 as the predominant respiratory lipoquinone. The major fatty acids of this strain were C18 : 1 ω7c (38.2 %) and C16 : 1 ω7c /C16 : 1 ω6c (17.4 %). The major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylcholine and phosphatidylglycerol. The DNA G+C content of strain T30T was 59.5 mol%. On the basis of phenotypic and phylogenetic data, a novel species, Erythrobacter nanhaisediminis sp. nov., is proposed; the type strain is T30T (=CGMCC 1.7715T=JCM 16125T).

scholarly journals Oceanicola nanhaiensis sp. nov., isolated from sediments of the South China Sea

2007 ◽  
Vol 57 (1) ◽  
pp. 157-160 ◽  
Author(s):  
Jun Gu ◽  
Bin Guo ◽  
Ya-Nan Wang ◽  
Su-Lin Yu ◽  
Ryuhei Inamori ◽  
...  
Keyword(s):  
South China Sea ◽  
South China ◽  
The South China Sea ◽  
Rrna Gene ◽  
The South ◽  
Respiratory Quinone ◽  
China Sea ◽  
Major Respiratory Quinone ◽  
Bacterium Strain ◽  
Sequence Similarities

A Gram-negative, non-motile, rod-shaped bacterium, strain SS011B1-20T, was isolated from sediments of the South China Sea. Growth occurred at NaCl concentrations between 0 and 10 % and at temperatures between 10 and 37 °C. Strain SS011B1-20T contained Q-10 as the major respiratory quinone and C18 : 1 ω7c (81.2 %), C16 : 0 (7.0 %) and C18 : 1 methyl (4.3 %) as the predominant fatty acids. The G+C content of the genomic DNA was 64.7 mol%. A phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain SS011B1-20T belonged to a clade within the genus Oceanicola in the Alphaproteobacteria, the highest sequence similarities being found with respect to Oceanicola batsensis (96.3 %) and with Oceanicola granulosus (94.9 %). Strain SS011B1-20T could be clearly distinguished from other Oceanicola species on the basis of the genotypic, phenotypic and phylogenetic data. Thus, it is proposed that strain SS011B1-20T represents a novel species of the genus Oceanicola, with the name Oceanicola nanhaiensis sp. nov. The type strain is SS011B1-20T (=LMG 23508T=CGMCC 1.6293T).

scholarly journals Cyclobacterium lianum sp. nov., a marine bacterium isolated from sediment of an oilfield in the South China Sea, and emended description of the genus Cyclobacterium

2006 ◽  
Vol 56 (12) ◽  
pp. 2927-2930 ◽  
Author(s):  
Jiao-Yan Ying ◽  
Bao-Jun Wang ◽  
Su-Sheng Yang ◽  
Shuang-Jiang Liu
Keyword(s):  
South China Sea ◽  
South China ◽  
The South China Sea ◽  
Rrna Gene ◽  
The South ◽  
Phenotypic Properties ◽  
China Sea ◽  
Emended Description ◽  
The 16S Rrna Gene ◽  
Sequence Similarities

The marine bacterial strain HY9T was isolated from sediment from the South China Sea. Strain HY9T is aerobic, heterotrophic and rose-pigmented. The cells are non-motile and curved, i.e. ring-like or horseshoe-shaped. The 16S rRNA gene sequence of strain HY9T was determined and blast searches revealed that it possessed significant sequence similarities with respect to Cyclobacterium species (92.8–93.6 %). Phylogenetic analysis confirmed that strain HY9T was tightly clustered with members of the genus Cyclobacterium. The cellular morphology and chemotaxonomic and phenotypic properties of strain HY9T showed that it should be classified as a member of the genus Cyclobacterium. Significant evolutionary distances and a range of phenotypic features distinguished strain HY9T from previously described Cyclobacterium species. Hence, strain HY9T represents a novel species in the genus Cyclobacterium, for which the name Cyclobacterium lianum sp. nov. is proposed. The type strain is HY9T (=CGMCC 1.6102T=JCM 14011T). On the basis of this study and previously described properties of Cyclobacterium species, an emended description of the genus Cyclobacterium is proposed.

scholarly journals Salegentibacter catena sp. nov., isolated from sediment of the South China Sea, and emended description of the genus Salegentibacter

2007 ◽  
Vol 57 (2) ◽  
pp. 219-222 ◽  
Author(s):  
Jiao-Yan Ying ◽  
Zhi-Pei Liu ◽  
Bao-Jun Wang ◽  
Xin Dai ◽  
Su-Sheng Yang ◽  
...  
Keyword(s):  
Fatty Acids ◽  
South China Sea ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
South China ◽  
The South China Sea ◽  
Rrna Gene ◽  
The South ◽  
China Sea ◽  
Emended Description

A novel marine bacterial strain, HY1T, was isolated from sediment of the South China Sea. The strain was aerobic and heterotrophic and formed saffron yellow-pigmented colonies on marine agar 2216. Cells were non-motile, Gram-negative rods, frequently occurring in chains. blastn searches revealed that the 16S rRNA gene sequence of strain HY1T showed high similarity with those of members of the genera Gillisia (91.7–93.8 %) and Salegentibacter (92.6–93.5 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain clustered with members of both Salegentibacter and Gillisia and phylogenetic trees constructed using three different methods (neighbour-joining, maximum-parsimony and minimum-evolution) indicated that strain HY1T clustered more frequently with members of the genus Salegentibacter. The DNA G+C content of strain HY1T was 44.4 mol% and its major cellular fatty acids (⩾5 % of the total fatty acids) were iso-15 : 1 (5.0 %), iso-15 : 0 (6.8 %), anteiso-15 : 0 (6.4 %), 15 : 0 (10.4 %), iso-16 : 0 (13.5 %), summed feature 3 (comprising iso-15 : 0 2-OH and/or 16 : 1ω7c; 6.3 %), iso-17 : 0 3-OH (5.2 %) and 17 : 0 2-OH (5.0 %). Cells contained menaquinone 6. Based on the phylogenetic and phenotypic analyses, strain HY1T should be classified as representing a novel species within the genus Salegentibacter, for which the name Salegentibacter catena sp. nov. is proposed. The type strain is HY1T (=CGMCC 1.6101T=JCM 14015T). Based on this study and on previously described Salegentibacter species, an emended description of the genus Salegentibacter is given.

scholarly journals Arenibacter nanhaiticus sp. nov., isolated from marine sediment of the South China Sea

2010 ◽  
Vol 60 (1) ◽  
pp. 78-83 ◽  
Author(s):  
Fengqin Sun ◽  
Baojiang Wang ◽  
Yaping Du ◽  
Xiupian Liu ◽  
Qiliang Lai ◽  
...  
Keyword(s):  
South China Sea ◽  
South China ◽  
Sequence Similarity ◽  
Optimal Growth ◽  
The South China Sea ◽  
Rrna Gene ◽  
Sandy Sediment ◽  
The South ◽  
China Sea ◽  
Gene Sequence Analysis

An aerobic, Gram-stain-negative, rod-shaped bacterial isolate, strain NH36AT, was isolated from a sandy sediment sample from the South China Sea. Colonies of the isolate were dark orange on M2 agar. Optimal growth was observed at pH 7.0–8.5, 30 °C and in the presence of 0.5–4.0 % (w/v) NaCl. The major fatty acids were C15 : 0, iso-C15 : 0, anteiso-C15 : 0, iso-C15 : 1, iso-C15 : 0 3-OH, iso-C17 : 0 3-OH and summed feature 3 (comprising iso-C15 : 0 2-OH and/or C16 : 1 ω7c). The DNA G+C content was 38.9 mol%. 16S rRNA gene sequence analysis revealed that strain NH36AT was most closely related to members of the genus Arenibacter, exhibiting 94.3–96.2 % sequence similarity to the type strains of Arenibacter species. On the basis of phenotypic, chemotaxonomic and phylogenetic data, this organism should be classified as a representative of a novel species in the genus Arenibacter. The name Arenibacter nanhaiticus sp. nov. is proposed and the type strain is NH36AT (=LMG 24842T=CCTCC AB 208315T=MCCC 1A04137T).

One new species and one new record of the species of the family Comesomatidae (Nematoda: Chromadorida) from the South China Sea

Zootaxa ◽  
2018 ◽  
Vol 4504 (1) ◽  
pp. 119 ◽  
Author(s):  
MIAN HUANG ◽  
SUSU JIA ◽  
YONG HUANG
Keyword(s):  
New Species ◽  
South China Sea ◽  
South China ◽  
The South China Sea ◽  
The East China Sea ◽  
The South ◽  
China Sea ◽  
The Family ◽  
Males And Females ◽  
One New Species

One new and one known species of the family Comesomatidae were collected from marine sediments in the South China Sea. Cervonema longispicula sp. nov. has stout cephalic setae, elongated and curved spicules with cephalated proximal end, and gubernaculum with hook-like dorsal apophysis that make the new species easily recognizable from the other 18 recorded Cervonema species. Paracomesoma zhangi Huang & Huang, 2018 was redescribed based on more specimens of males and females. These specimens are in accordance with the characteristics of holotype from the East China Sea except having more precloacal supplements (30–39), males slightly smaller (not longer than 1710 µm) and with shorter cephalic setae (not longer than 10 μm). 

scholarly journals Marinobacter segnicrescens sp. nov., a moderate halophile isolated from benthic sediment of the South China Sea

2007 ◽  
Vol 57 (9) ◽  
pp. 1970-1974 ◽  
Author(s):  
Bin Guo ◽  
Jun Gu ◽  
Yu-Guang Ye ◽  
Yue-Qin Tang ◽  
Kenji Kida ◽  
...  
Keyword(s):  
South China Sea ◽  
South China ◽  
Carbon Sources ◽  
The South China Sea ◽  
Rrna Gene ◽  
Organic Substrates ◽  
The South ◽  
Respiratory Quinone ◽  
Moderate Halophile ◽  
China Sea

A Gram-negative, motile, non-spore-forming and moderately halophilic ellipsoid-shaped marine coccobacillus, designated strain SS011B1-4T, was isolated from benthic sediment of the South China Sea. Optimum growth occurred at 30–37 °C, pH 7.5–8.0 and 4–8 % (w/v) NaCl. Strain SS011B1-4T utilized a variety of organic substrates as sole carbon sources, but did not utilize toluene, n-tetradecane or crude oil. Strain SS011B1-4T had ubiquinone-9 as the major respiratory quinone and C18 : 1 ω9c, C16 : 0 and C12 : 0 3-OH as the predominant fatty acids. The genomic DNA G+C content was 62.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain SS011B1-4T belonged to the genus Marinobacter of the Gammaproteobacteria. The results of the phenotypic, phylogenetic and genomic analyses revealed that strain SS011B1-4T represents a novel species of the genus Marinobacter. The name Marinobacter segnicrescens sp. nov. is therefore proposed, with strain SS011B1-4T (=LMG 23928T=CGMCC 1.6489T) as the type strain.

scholarly journals Zhouia amylolytica gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from sediment of the South China Sea

2006 ◽  
Vol 56 (12) ◽  
pp. 2825-2829 ◽  
Author(s):  
Zhi-Pei Liu ◽  
Bao-Jun Wang ◽  
Xin Dai ◽  
Xing-Yu Liu ◽  
Shuang-Jiang Liu
Keyword(s):  
South China Sea ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
South China ◽  
The South China Sea ◽  
Rrna Gene ◽  
Gene Sequences ◽  
16S Rrna Gene Sequences ◽  
China Sea ◽  
The Family

Three Gram-negative, non-spore-forming strains were isolated from sediment from the South China Sea, China, and their taxonomic positions were investigated using a polyphasic approach. Strains HN-171T, HN-172 and HN-181 grew optimally at 30 °C, in the presence of 4.5–5.0 NaCl % (w/v) and at pH 7.2–7.4. They contained MK-6 as the predominant respiratory quinone and contained iso-C15 : 1 G, iso-C15 : 0, summed feature 4 (iso-C15 : 0 2-OH and/or C16 : 1 ω7c/t) and C15 : 0 as the major fatty acids. The DNA G+C content of strain HN-171T was 34.5 mol%. Phylogenetic analyses based on 16S rRNA gene sequences demonstrated that strain HN-171T, together with strains HN-172 and HN-181, formed a distinct evolutionary lineage within the family Flavobacteriaceae. The 16S rRNA gene sequences of strains HN-171T, HN-172 and HN-181 shared 99.8–100 % similarity with each other, and the sequence of strain HN-171T exhibited similarity values below 90.2 % with those of other members of the family Flavobacteriaceae. The closest relative of HN-171T was Coenonia anatina LMG 14382T (90.2 %). On the basis of their phenotypic and phylogenetic properties, the three isolates represent a novel genus and a novel species, for which the name Zhouia amylolytica gen. nov., sp. nov. is proposed. The type strain is HN-171T (=CGMCC 1.6114T=JCM 14016T).

scholarly journals Puteibacter caeruleilacunae gen. nov., sp. nov., a facultatively anaerobic bacterium isolated from Yongle Blue Hole in the South China Sea

2020 ◽  
Vol 70 (3) ◽  
pp. 1623-1629 ◽  
Author(s):  
Wen Sun ◽  
Tianyu Fu ◽  
Chao Jia ◽  
Liang Fu ◽  
Shun Zhou ◽  
...  
Keyword(s):  
South China Sea ◽  
South China ◽  
The South China Sea ◽  
The South ◽  
Content Type ◽  
China Sea ◽  
Link Type ◽  
Facultatively Anaerobic ◽  
Blue Hole ◽  
The Family

Blue holes are unique geomorphological units characterized by steep redox and biogeochemical gradients. Yongle Blue Hole is located on the largest atoll (Yongle Atoll) of the western Xisha Islands in the South China Sea. A Gram-stain-negative, facultatively anaerobic, non-motile, non-flagellated marine bacterium with creamy white colonies, designated JC036T, was isolated from Yongle Blue Hole. Cells were short-rod-shaped and catalase-negative. 16S rRNA gene sequence analysis showed that sequence similarities were lower than 91.6 % against all validly named species in the family Prolixibacteraceae ; a reconstructed phylogenetic tree indicated that strain JC036T formed a lineage with strains in the family Prolixibacteraceae . Growth occurred at 4–37 °C (optimum, 28 °C), at pH 5.0–9.0 (optimum, 7.0) and in the presence of 2–6 % (w/v) NaCl (optimum, 3 %). The prevalent isoprenoid quinone of strain JC036T was menaquinone-7 (MK-7). Iso-C15 : 0 and iso-C17 : 0 3-OH were the predominant fatty acids. The major polar lipids included a phospholipid, phosphatidylethanolamine, an aminophospholipid and four unidentified lipids. The genomic DNA G+C content of strain JC036T was 37.8 mol%. Based on physiological and biochemical characteristics and whole genome comparisons, we propose a new genus and species, Puteibacter caeruleilacunae gen. nov., sp. nov., within the family Prolixibacteraceae . The type strain of Puteibacter caeruleilacunae is JC036T (=JCM 33128T=MCCC 1K03579T). From this study, a deeper understanding of the community of the microorganism and their roles in biogeochemical cycles, especially anaerobic bacteria, is provided.

scholarly journals Oceanicola nitratireducens sp. nov., a marine alphaproteobacterium isolated from the South China Sea

2010 ◽  
Vol 60 (7) ◽  
pp. 1655-1659 ◽  
Author(s):  
Qiang Zheng ◽  
Chuang Chen ◽  
Ya-Nan Wang ◽  
Nianzhi Jiao
Keyword(s):  
South China Sea ◽  
South China ◽  
Phylogenetic Analyses ◽  
The South China Sea ◽  
Rrna Gene ◽  
Beibu Gulf ◽  
16S Rrna Gene Sequences ◽  
The South ◽  
Phenotypic Data ◽  
China Sea

A Gram-negative, non-motile, short-rod-shaped bacterial strain (JLT1210T) that accumulates poly-β-hydroxybutyrate granules was isolated from the Beibu Gulf in the South China Sea. Cells have polar or subpolar flagella. Phylogenetic analyses based on 16S rRNA gene sequences showed that the strain belongs to the genus Oceanicola in the order Rhodobacterales, class Alphaproteobacteria. The closest neighbours were Oceanicola nanhaiensis SS011B1-20T (96.5 % similarity) and Oceanicola batsensis HTCC2597T (96.4 %). The predominant respiratory ubiquinone of strain JLT1210T was Q-10 and the DNA G+C content was 72.8 mol%. Evidence from genotypic, chemotaxonomic and phenotypic data shows that strain JLT1210T represents a novel species of the genus Oceanicola, for which the name Oceanicola nitratireducens sp. nov. is proposed; the type strain is JLT1210T (=LMG 24663T=CGMCC 1.7292T).

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